CASS4 Gene
protein-coding GIFtS: 48
GCID: GC20P054987
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Cas scaffolding protein family member 4(Previous name: chromosome 20 open reading frame 32 )
(Previous symbol: C20orf32)
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Aliases
for CASS4 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Cas Scaffolding Protein Family Member 41 2 | | HEF-Like Protein2 3 | | C20orf321 2 3 | | HEF1-EFS-P130Cas-Like Protein2 3 | | HEFL1 2 3 | | Chromosome 20 Open Reading Frame 321 | | HEPL1 2 3 | | CAS42 |
Export aliases for CASS4 gene to outside databasesPrevious GC identifers: GC20P054421 GC20P051772 |
Summaries
for CASS4 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: CASS4_HUMAN, Q9NQ75Function: Docking protein that plays a role in tyrosine kinase-based signaling related to cell adhesion and cellspreading. Regulates PTK2/FAK1 activity, focal adhesion integrity, and cell spreading
|
Genomic Views
for CASS4 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000020.10 NC_018931.1 NT_011362.10
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the CASS4 gene promoter:
AML1a GCNF Lmo2 Meis-1b FAC1 GCNF-1 Meis-1a GCNF-2 Meis-1
Other transcription factors
Search SABiosciences Chromatin IP Primers for CASS4
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CASS4 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 20q13.31 Ensembl cytogenetic band: 20q13.2 HGNC cytogenetic band: 20q13.31CASS4 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 20 GeneLoc Exon Structure GeneLoc location for GC20P054987: view genomic region
(about GC identifiers)
Start:
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54,987,168 bp from pter |
End:
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55,034,396 bp from pter |
Size:
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47,229 bases |
Orientation:
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plus strand |
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Proteins
for CASS4 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: CASS4_HUMAN, Q9NQ75 (See
protein sequence)Recommended Name: Cas scaffolding protein family member 4 Size: 786 amino acids; 87144 Da
Subunit: Interacts (via SH3 domain) with PTK2/FAK1 (via C-terminus)
Subcellular location: Cytoplasm, cytoskeleton. Cell junction, focal adhesion
Sequence caution: Sequence=CAC00655.1; Type=Frameshift; Positions=336, 342;
1 PDB 3D structure from  and Proteopedia  for CASS4:2CRE (3D)
Secondary accessions: E1P5Z8 Q5QPD6 Q96K09 Q9BYL5Alternative splicing: 3 isoforms: Q9NQ75-1 Q9NQ75-2 Q9NQ75-3 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for CASS4: NX_Q9NQ75
Post-translational modifications:
Phosphorylated on tyrosines by SRC1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9NQ75
CASS4 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (4 alternative transcripts):
NP_001157586.1 NP_001157587.1 NP_001157588.1 NP_065089.2
ENSEMBL proteins: ENSP00000353462 ENSP00000410027 ENSP00000360387
Human Recombinant Protein Products:
Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table
CASS4 for ontologies About GeneDecksing
CASS4 Antibody Products:
Assay Products for CASS4: |
Protein
Domains /
Families
for CASS4 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
CASS4 for domains About GeneDecksing
4 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q9NQ75ProtoNet protein and cluster: Q9NQ75 1 Blocks protein family: IPB001452 SH3 domain signature
UniProtKB/Swiss-Prot: CASS4_HUMAN, Q9NQ75Similarity: Belongs to the CAS familySimilarity: Contains 1 SH3 domain |
Function
for CASS4 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: CASS4_HUMAN, Q9NQ75Function: Docking protein that plays a role in tyrosine kinase-based signaling related to cell adhesion and cellspreading. Regulates PTK2/FAK1 activity, focal adhesion integrity, and cell spreading
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CASS4 (see all 6)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CASS4 (see all 4)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 4): CASS4 (NM_020356) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CASS4 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CASS4 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CASS4 |
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0000155 | phosphorelay sensor kinase activity |
IEA | -- |
CASS4 for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for CASS4:
|
Pathways & Interactions
for CASS4 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CASS4
STRING Interaction
Network Preview (showing 1 interactants - click image to see more details)
1 Interacting protein for CASS4 (Q9NQ753 ENSP000003534624) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0007155 | cell adhesion |
IEA | -- |
CASS4 for ontologies About GeneDecksing
|
Drugs & Compounds
for CASS4 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for CASS4
Search CenterWatch for drugs/clinical trials and news about CASS4
|
Transcripts
for CASS4 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for CASS4 gene (4 alternative transcripts): NM_001164114.1 NM_001164115.1 NM_001164116.1 NM_020356.3 Unigene Cluster for CASS4: Cas scaffolding protein family member 4 Hs.473144 [show with all ESTs]Unigene Representative Sequence: NM_0011641164 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000360314 ENST00000434344 ENST00000497244(uc002xxq.4) ENST00000371336(uc002xxp.2 uc010zze.1 uc002xxr.2 uc010gio.2)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CASS4 (see all 6)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CASS4 (see all 4)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 4): CASS4 (NM_020356) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CASS4 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CASS4 |
Additional cDNA sequence: AJ276678.1 AK027760.1 AK295613.1 AK308057.1 AK314653.1 BC027951.1 5 DOTS entries: DT.97781524 DT.92012542 DT.120801562 DT.100646240 DT.200911 7 AceView cDNA sequences: BC027951 BX280195 AK027760 AJ276678 BI768239 CB154772 NM_020356
GeneLoc Exon Structure
5/6 Alternative Splicing Database (ASD) splice patterns (SP) for CASS4 (see all 6) About this scheme
| ExUns: | 1 | ^ | 2a | · | 2b | · | 2c | · | 2d | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6 | ^ | 7 | ^ | 8a | · | 8b | ^ | 9a | · | 9b | · | 9c | ^ | 10a | · | 10b |
|---|
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| SP1: | | | - | | - | | | | | | - | | | | | | - | | | | | | | | | | | | - | | | | | | |
| SP2: | | | | | | | | | | | - | | | | | | - | | | | | | | | - | | - | | - | | | | | | |
| SP3: | | | | | | | | | | | - | | | | | | - | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | - | | - | | | | - | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for CASS4
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Expression
for CASS4 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| CASS4 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GTGGCACGGA
About this image
See CASS4 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for CASS4
SOURCE GeneReport for Unigene cluster: Hs.473144
UniProtKB/Swiss-Prot: CASS4_HUMAN, Q9NQ75Tissue specificity: Expressed abundantly in lung and spleen. Also highly expressed in ovarian and leukemia cell lines
SABiosciences Custom PCR Arrays for CASS4
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CASS4
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat CASS4 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CASS4 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CASS4 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CASS4 |
Orthologs
for CASS4 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for CASS4 gene from 5/18 species (see all 18) About this table
ENSEMBL Gene Tree for CASS4 (if available)
TreeFam Gene Tree for CASS4 (if available) |
Paralogs
for CASS4 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for CASS4 gene
- BCAR12 NEDD92 EFS2
1 SIMAP similar gene for CASS4 using alignment to 1 protein entry: CASS4_HUMAN:SH3GL2
CASS4 for paralogs About GeneDecksing
|
Genomic Variants
for CASS4 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 20 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for CASS4 (54987168 - 55034396 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 5 variations for CASS4
2 CNVs: 90622 67715
3 Indels: 79245 90620 90621 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CASS4 |
|
Disorders
/ Diseases
for CASS4 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
CASS4 for disorders About GeneDecksing
4  diseases for CASS4: About MalaCardshypervitaminosis a estrogen resistance breast cancer leukemia
1 disease from the University of Copenhagen DISEASES database for CASS4:Hypervitaminosis A Export disorders for CASS4 gene to outside databases
|
Publications
for CASS4 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for CASS4 gene integrated from 9 sources:
(articles sorted by number of sources associating them with CASS4) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- A novel Cas family member, HEPL, regulates FAK and cell spreading. (PubMed id 18256281)1, 2 Singh M.K.... Golemis E.A. (2008)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (2001)
- Charting the molecular network of the drug target Bcr-Abl. (PubMed id 19380743)1 Brehme M....Superti-Furga G. (2009)
- Phosphoproteome of resting human platelets. (PubMed id 18088087)2 Zahedi R.P.... Sickmann A. (2008)
- Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (2006)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
- Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )2 Aoki H.... Hino O. (2000)
|
External Searches for CASS4 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing CASS4 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing CASS4 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing CASS4 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for CASS4 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for CASS4 gene:
Search GeneIP for patents involving CASS4
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for CASS4 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for CASS4 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CASS4 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CASS4 | | | OriGene Protein Over-expression Lysate for CASS4 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for CASS4 | | OriGene 3'-UTR Clone for CASS4 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CASS4 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CASS4 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for CASS4 | | OriGene Custom Protein Services for CASS4 | | | OriGene Custom Immunoassay Development | | |
| |
 |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat CASS4 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CASS4 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CASS4 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CASS4 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CASS4 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CASS4 |
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 |  |  |  | |
| | | | Search Tocris compounds for CASS4 |
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 | | | CASS4 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CASS4 |
|  |  |  | | | | Search ThermoFisher Antibodies for CASS4 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CASS4 |
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