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Aliases for CASP3 Gene

Aliases for CASP3 Gene

  • Caspase 3 2 3 5
  • Caspase 3, Apoptosis-Related Cysteine Peptidase 2 3
  • Caspase 3, Apoptosis-Related Cysteine Protease 2 3
  • SREBP Cleavage Activity 1 3 4
  • Cysteine Protease CPP32 3 4
  • Protein Yama 3 4
  • EC 3.4.22.56 4 63
  • Apopain 3 4
  • CASP-3 3 4
  • CPP-32 3 4
  • CPP32 3 4
  • SCA-1 3 4
  • PARP Cleavage Protease 3
  • Procaspase3 3
  • EC 3.4.22 63
  • CPP32B 3

External Ids for CASP3 Gene

Previous GeneCards Identifiers for CASP3 Gene

  • GC04M186576
  • GC04M186228
  • GC04M186133
  • GC04M186244
  • GC04M185924
  • GC04M185785
  • GC04M185548
  • GC04M181303

Summaries for CASP3 Gene

Entrez Gene Summary for CASP3 Gene

  • This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

GeneCards Summary for CASP3 Gene

CASP3 (Caspase 3) is a Protein Coding gene. Diseases associated with CASP3 include monocytic leukemia and colon adenocarcinoma. Among its related pathways are AGE-RAGE signaling pathway in diabetic complications and Alzheimer's disease. GO annotations related to this gene include peptidase activity and cysteine-type peptidase activity. An important paralog of this gene is CASP1.

UniProtKB/Swiss-Prot for CASP3 Gene

  • Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a 216-Asp- -Gly-217 bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

Tocris Summary for CASP3 Gene

  • Caspases (cysteinyl aspartate proteases) are involved in the signaling pathways of apoptosis, necrosis and inflammation. These enzymes can be divided into initiators and effectors. The initiator isoforms are activated by, and interact with, upstream adaptor molecules.

Gene Wiki entry for CASP3 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CASP3 Gene

Genomics for CASP3 Gene

Regulatory Elements for CASP3 Gene

Enhancers for CASP3 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around CASP3 on UCSC Golden Path with GeneCards custom track

Promoters for CASP3 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around CASP3 on UCSC Golden Path with GeneCards custom track

Genomic Location for CASP3 Gene

Chromosome:
4
Start:
184,627,696 bp from pter
End:
184,649,509 bp from pter
Size:
21,814 bases
Orientation:
Minus strand

Genomic View for CASP3 Gene

Genes around CASP3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CASP3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CASP3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CASP3 Gene

Proteins for CASP3 Gene

  • Protein details for CASP3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P42574-CASP3_HUMAN
    Recommended name:
    Caspase-3
    Protein Accession:
    P42574
    Secondary Accessions:
    • A8K5M2
    • D3DP53
    • Q96AN1
    • Q96KP2

    Protein attributes for CASP3 Gene

    Size:
    277 amino acids
    Molecular mass:
    31608 Da
    Quaternary structure:
    • Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce.

    Three dimensional structures from OCA and Proteopedia for CASP3 Gene

neXtProt entry for CASP3 Gene

Proteomics data for CASP3 Gene at MOPED

Post-translational modifications for CASP3 Gene

  • Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
  • S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Ubiquitination at Lys 229
  • Modification sites at PhosphoSitePlus

Other Protein References for CASP3 Gene

Assay Products

  • Enzo Life Sciences assays for CASP3
  • Cloud-Clone Corp Assay Kits for CASP3

Domains & Families for CASP3 Gene

Gene Families for CASP3 Gene

HGNC:
IUPHAR :

Suggested Antigen Peptide Sequences for CASP3 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P42574

UniProtKB/Swiss-Prot:

CASP3_HUMAN :
  • Belongs to the peptidase C14A family.
Family:
  • Belongs to the peptidase C14A family.
genes like me logo Genes that share domains with CASP3: view

Function for CASP3 Gene

Molecular function for CASP3 Gene

GENATLAS Biochemistry:
caspase 3,effector,cysteine containing aspartate-specific protease CPP32,activated by CASP9,cleaving specifically CDKN1A,alpha-fodrin (SPTAN1), gelsolin (GSN),stored in the mitochondrial intermembrane space and released into cytosol after appropriate apoptotic stimuli,promoting CD95/APO1/fas mediated apoptosis,homolog to C elegans Ced-3 and mammalian interleukin 1 beta convertase (IL1BC),including two isoforms alpha and beta
UniProtKB/Swiss-Prot CatalyticActivity:
Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp- - with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.
UniProtKB/Swiss-Prot EnzymeRegulation:
Inhibited by isatin sulfonamides.
UniProtKB/Swiss-Prot Function:
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a 216-Asp- -Gly-217 bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

Enzyme Numbers (IUBMB) for CASP3 Gene

Gene Ontology (GO) - Molecular Function for CASP3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004861 cyclin-dependent protein serine/threonine kinase inhibitor activity IEA --
GO:0008234 cysteine-type peptidase activity IEA --
GO:0016005 phospholipase A2 activator activity IEA --
genes like me logo Genes that share ontologies with CASP3: view
genes like me logo Genes that share phenotypes with CASP3: view

Animal Models for CASP3 Gene

MGI Knock Outs for CASP3:

Animal Model Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CASP3 Gene

Localization for CASP3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CASP3 Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for CASP3 Gene COMPARTMENTS Subcellular localization image for CASP3 gene
Compartment Confidence
cytosol 5
nucleus 5
plasma membrane 5
mitochondrion 4
cytoskeleton 3
endoplasmic reticulum 3
extracellular 3
lysosome 2
vacuole 2
endosome 1
golgi apparatus 1
peroxisome 1

No data available for Gene Ontology (GO) - Cellular Components for CASP3 Gene

Pathways & Interactions for CASP3 Gene

genes like me logo Genes that share pathways with CASP3: view

Pathways by source for CASP3 Gene

SIGNOR curated interactions for CASP3 Gene

Activates:
Inactivates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for CASP3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001666 response to hypoxia IEA --
GO:0001782 B cell homeostasis IEA --
GO:0006309 apoptotic DNA fragmentation TAS --
GO:0006508 proteolysis IEA,IDA 12888622
GO:0006915 apoptotic process IEA,TAS 7983002
genes like me logo Genes that share ontologies with CASP3: view

Drugs & Compounds for CASP3 Gene

(77) Drugs for CASP3 Gene - From: DrugBank, ApexBio, DGIdb, IUPHAR, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Cisplatin Approved Pharma Inhibits DNA synthesis,chemotherapy drug, Platinum 2569
Minocycline Approved, Investigational Pharma Target, negative modulator 210
(1S)-1-(3-chlorophenyl)-2-oxo-2-[(1,3,4-trioxo-1,2,3,4-tetrahydroisoquinolin-5-yl)amino]ethyl acetate Experimental Pharma Target 0
(1S)-2-oxo-1-phenyl-2-[(1,3,4-trioxo-1,2,3,4-tetrahydroisoquinolin-5-yl)amino]ethyl acetate Experimental Pharma Target 0
[N-(3-DIBENZYLCARBAMOYL-OXIRANECARBONYL)-HYDRAZINO]-ACETIC ACID Experimental Pharma Target 0

(82) Additional Compounds for CASP3 Gene - From: Novoseek and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Calpeptin
117591-20-5
MG 132
133407-82-6
N-Acetyl-L-leucyl-L-leucyl-L-methional
136632-32-1
PD 150606
179528-45-1

(5) Tocris Compounds for CASP3 Gene

Compound Action Cas Number
Calpeptin Calpain and cathepsin L inhibitor 117591-20-5
MDL 28170 Potent, selective calpain and cathepsin B inhibitor 88191-84-8
MG 132 Proteasome and calpain inhibitor. Inhibits NF-kappaB activation 133407-82-6
N-Acetyl-L-leucyl-L-leucyl-L-methional Cathepsin inhibitor 136632-32-1
PD 150606 Cell permeable calpain inhibitor 179528-45-1

(11) ApexBio Compounds for CASP3 Gene

Compound Action Cas Number
Apoptosis Activator 2 Indoledione caspase activator, cell-permeable 79183-19-0
Apoptosis Inhibitor Associate with caspase-3 inhibition 54135-60-3
Boc-D-FMK Pan-caspase inhibitor 187389-53-3, 634911-80-1
Caspase-3/7 Inhibitor I Caspase-3/7 inhibitor 220509-74-0
Cisplatin Inhibits DNA synthesis,chemotherapy drug 15663-27-1
Gambogic Acid Caspase activator and apoptosis inducer 2752-65-0
PAC-1 Procaspase-3 activator 315183-21-2
Q-VD-OPh hydrate Cell-permeable, irreversible pan-caspase inhibitor 1135695-98-5
Tasisulam 519055-62-0
Z-DEVD-FMK Caspase-3 inhibitor 210344-95-9
Z-VAD-FMK Cell-permeable, irreversible pan-caspase inhibitor 187389-52-2
genes like me logo Genes that share compounds with CASP3: view

Drug Products

Transcripts for CASP3 Gene

Unigene Clusters for CASP3 Gene

Caspase 3, apoptosis-related cysteine peptidase:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for CASP3 Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7 ^ 8a · 8b
SP1: -
SP2: - - -
SP3: -
SP4: -

Relevant External Links for CASP3 Gene

GeneLoc Exon Structure for
CASP3
ECgene alternative splicing isoforms for
CASP3

Expression for CASP3 Gene

mRNA expression in normal human tissues for CASP3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for CASP3 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (12.2) and Nasal epithelium (11.9).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for CASP3 Gene



SOURCE GeneReport for Unigene cluster for CASP3 Gene Hs.141125

mRNA Expression by UniProt/SwissProt for CASP3 Gene

P42574-CASP3_HUMAN
Tissue specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
genes like me logo Genes that share expression patterns with CASP3: view

Protein tissue co-expression partners for CASP3 Gene

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for CASP3 Gene

Orthologs for CASP3 Gene

This gene was present in the common ancestor of animals.

Orthologs for CASP3 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia CASP3 35
  • 86.3 (n)
  • 84 (a)
CASP3 36
  • 84 (a)
OneToOne
dog
(Canis familiaris)
Mammalia CASP3 35
  • 89.53 (n)
  • 89.17 (a)
CASP3 36
  • 82 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Casp3 35
  • 83.63 (n)
  • 86.64 (a)
Casp3 16
Casp3 36
  • 87 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia CASP3 35
  • 99.52 (n)
  • 99.64 (a)
CASP3 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Casp3 35
  • 84.84 (n)
  • 89.17 (a)
oppossum
(Monodelphis domestica)
Mammalia -- 36
  • 78 (a)
OneToMany
-- 36
  • 71 (a)
OneToMany
-- 36
  • 72 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia CASP3 36
  • 68 (a)
OneToOne
chicken
(Gallus gallus)
Aves CASP3 35
  • 70.76 (n)
  • 66.91 (a)
CASP3 36
  • 64 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia casp3 35
  • 66.67 (n)
  • 63.07 (a)
zebrafish
(Danio rerio)
Actinopterygii casp3 35
casp3a 36
  • 55 (a)
OneToMany
casp3b 36
  • 53 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta Dcp-1 37
  • 39 (a)
decay 37
  • 37 (a)
Ice 37
  • 42 (a)
Dcp-1 36
  • 30 (a)
ManyToMany
decay 36
  • 30 (a)
ManyToMany
Ice 36
  • 32 (a)
ManyToMany
African malaria mosquito
(Anopheles gambiae)
Insecta CASPS8 35
  • 45.94 (n)
  • 37.08 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 39 (a)
ManyToMany
-- 36
  • 33 (a)
ManyToMany
-- 36
  • 38 (a)
ManyToMany
-- 36
  • 36 (a)
ManyToMany
-- 36
  • 40 (a)
ManyToMany
-- 36
  • 34 (a)
ManyToMany
-- 36
  • 32 (a)
ManyToMany
-- 36
  • 39 (a)
ManyToMany
-- 36
  • 23 (a)
ManyToMany
-- 36
  • 47 (a)
ManyToMany
Species with no ortholog for CASP3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for CASP3 Gene

ENSEMBL:
Gene Tree for CASP3 (if available)
TreeFam:
Gene Tree for CASP3 (if available)

Paralogs for CASP3 Gene

Paralogs for CASP3 Gene

(6) SIMAP similar genes for CASP3 Gene using alignment to 3 proteins:

Pseudogenes.org Pseudogenes for CASP3 Gene

genes like me logo Genes that share paralogs with CASP3: view

Variants for CASP3 Gene

Sequence variations from dbSNP and Humsavar for CASP3 Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type
rs1049210 - 184,631,071(-) GTGGA(G/T)GCCGA reference, missense
rs35578277 - 184,635,407(-) CATAC(A/G)TGGAA reference, missense
rs6948 -- 184,627,976(-) TGAGC(A/C)CTCAG utr-variant-3-prime
rs8549 -- 184,627,854(-) CTCGC(A/G)TTTGT utr-variant-3-prime
rs1049216 -- 184,628,935(-) AGTAA(C/T)GAATT utr-variant-3-prime

Structural Variations from Database of Genomic Variants (DGV) for CASP3 Gene

Variant ID Type Subtype PubMed ID
nsv830168 CNV Loss 17160897

Variation tolerance for CASP3 Gene

Residual Variation Intolerance Score: 53.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.87; 18.17% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for CASP3 Gene

HapMap Linkage Disequilibrium report
CASP3
Human Gene Mutation Database (HGMD)
CASP3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CASP3 Gene

Disorders for CASP3 Gene

MalaCards: The human disease database

(78) MalaCards diseases for CASP3 Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
monocytic leukemia
  • schilling's leukemia
colon adenocarcinoma
  • adenocarcinoma of colon
pancreatic cancer
  • pancreatic carcinoma, somatic
oral lichen planus
  • oral lichenoid lesions
spinal cord injury
  • spinal cord injuries
- elite association - COSMIC cancer census association via MalaCards
Search CASP3 in MalaCards View complete list of genes associated with diseases

Relevant External Links for CASP3

Genetic Association Database (GAD)
CASP3
Human Genome Epidemiology (HuGE) Navigator
CASP3
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
CASP3
genes like me logo Genes that share disorders with CASP3: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CASP3 Gene

Publications for CASP3 Gene

  1. Structure of recombinant human CPP32 in complex with the tetrapeptide acetyl-Asp-Val-Ala-Asp fluoromethyl ketone. (PMID: 9045680) Mittl P.R.E. … Gruetter M.G. (J. Biol. Chem. 1997) 3 4 23 67
  2. Caspase-3 and -9 are activated in human myeloid HL-60 cells by calcium signal. (PMID: 19626422) GonzA!lez D. … Pariente J.A. (Mol. Cell. Biochem. 2010) 3 23
  3. Involvement of p21Waf1/Cip1 cleavage during roscovitine-induced apoptosis in non-small cell lung cancer cells. (PMID: 19956888) Zhang T. … Li X.F. (Oncol. Rep. 2010) 3 23
  4. Constitutive activation of caspase-3 and Poly ADP ribose polymerase cleavage in fanconi anemia cells. (PMID: 20068062) Lyakhovich A. … SurrallAcs J. (Mol. Cancer Res. 2010) 3 23
  5. A novel small molecule, LLL12, inhibits STAT3 phosphorylation and activities and exhibits potent growth-suppressive activity in human cancer cells. (PMID: 20072652) Lin L. … Lin J. (Neoplasia 2010) 3 23

Products for CASP3 Gene

  • Addgene plasmids for CASP3

Sources for CASP3 Gene

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