CASP3 Gene
protein-coding GIFtS: 73
GCID: GC04M185548
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|
caspase 3, apoptosis-related cysteine peptidase(Previous names: caspase 3, apoptosis-related cysteine protease )
| |
Aliases
for CASP3 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Caspase 3, Apoptosis-Related Cysteine Peptidase1 2 | | CPP-322 3 | | CPP321 2 3 5 | | SCA-12 3 | | CPP32B1 2 | | SREBP Cleavage Activity 12 3 | | Yama1 2 | | EC 3.4.22.563 8 | | Apopain3 | | Caspase-31 | | Caspase 3, Apoptosis-Related Cysteine Protease1 2 | | PARP Cleavage Protease2 | | Cysteine Protease CPP322 3 | | Procaspase31 | | Protein Yama2 3 | | Apopain3 | | CASP-32 3 | | EC 3.4.228 |
Export aliases for CASP3 gene to outside databasesPrevious GC identifers: GC04M186576 GC04M186228 GC04M186133 GC04M186244 GC04M185924 GC04M185785 GC04M181303 |
Summaries
for CASP3 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for CASP3:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequentialactivation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactiveproenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large andsmall, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and theprotein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage ofamyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternativesplicing of this gene results in two transcript variants that encode the same protein. (provided by RefSeq, Jul 2008)
UniProtKB/Swiss-Prot: CASP3_HUMAN, P42574Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosisit proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activatessterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and themembrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.Triggers cell adhesion in sympathetic neurons through RET cleavage
 summary
for CASP3: Caspases (short for 'cysteinyl aspartate proteases') are involved in the signal transduction pathways of
apoptosis, necrosis and inflammation. These enzymes can be divided into two major classes - initiators and
effectors. The initiator isoforms (caspases-1,-4,-5,-8,-9,-10,-11,-12) are activated by, and interact with,
upstream adaptor molecules through protein-protein interaction domains known as CARD and DED. Effector
caspases (-3,-6,-7) are responsible for cleaving downstream substrates and are sometimes referred to as the
executioner caspases. More than 400 caspase substrates have so far been identified (see The Caspase
Substrate Database). Initiator caspases, such as caspase 8, may be directly activated by death receptors
such as FasR. Caspases can also be found intracellularly as part of large multiprotein complexes. For
example, caspase 9 is recruited to the apoptosome formed during apoptosis, whilst caspases-1 and 5 can form
part of the inflammasome, a key part of cytokine processing during inflammation. Caspases are regulated by
inhibitors of apoptosis and by dominant negative isoforms. They have been implicated in the pathogenesis of
many disorders including stroke, Alzheimer's disease, myocardial infarction, cancer, and inflammatory
disease.
Gene Wiki entry for CASP3 (Caspase 3)
|
Genomic Views
for CASP3 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000004.11 NC_018915.1 NT_016354.19
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the CASP3 gene promoter:
PPAR-alpha AP-1 ATF-2 c-Jun FOXO1
Other transcription factors
Search SABiosciences Chromatin IP Primers for CASP3
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CASP3 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 4q34 Ensembl cytogenetic band: 4q35.1 HGNC cytogenetic band: 4q34CASP3 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 4 GeneLoc Exon Structure GeneLoc location for GC04M185548: view genomic region
(about GC identifiers)
Start:
|
185,548,850 bp from pter |
End:
|
185,570,663 bp from pter |
Size:
|
21,814 bases |
Orientation:
|
minus strand |
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Proteins
for CASP3 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: CASP3_HUMAN, P42574 (See
protein sequence)Recommended Name: Caspase-3 precursor Size: 277 amino acids; 31608 Da
Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) anda 12 kDa (p12) subunit. Interacts with BIRC6/bruce
Subcellular location: Cytoplasm
6/65 PDB 3D structures from  and Proteopedia  for CASP3 (see all 65):1CP3 (3D)
1GFW (3D)
1I3O (3D)
1NME (3D)
1NMQ (3D)
1NMS (3D)
Secondary accessions: A8K5M2 D3DP53 Q96AN1 Q96KP2Explore the universe of human proteins at neXtProt for CASP3: NX_P42574
Post-translational modifications:
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing ofthe propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between thesmall subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa1
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation ofthe Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activatescaspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating thedenitrosylation of its active site thiol1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_P42574
4/14 DME Specific Peptides for CASP3 (P42574) (see all 14)
CASP3 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_004337.2 NP_116786.1
ENSEMBL proteins: ENSP00000311032 ENSP00000377210 ENSP00000428929 ENSP00000428372 ENSP00000377213 ENSP00000407142 Reactome Protein details: P42574
Human Recombinant Protein Products:
Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7): About this table
CASP3 for ontologies About GeneDecksing
CASP3 Antibody Products:
Assay Products for CASP3: |
Protein
Domains /
Families
for CASP3 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
CASP3 for domains About GeneDecksing
5/7 InterPro domains/families (see all 7):Graphical View of Domain Structure for InterPro Entry P42574ProtoNet protein and cluster: P42574 2 Blocks protein families: IPB001309 Caspase-1 IPB002398 Interleukin-1B converting enzyme signature
UniProtKB/Swiss-Prot: CASP3_HUMAN, P42574Similarity: Belongs to the peptidase C14A family |
Function
for CASP3 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: CASP3_HUMAN, P42574Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosisit proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activatessterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and themembrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.Triggers cell adhesion in sympathetic neurons through RET cleavageCatalytic activity: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequenceof Asp-Xaa-Xaa-Asp- - with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, althoughVal or Ala are also accepted at this positionEnzyme regulation: Inhibited by isatin sulfonamides Genatlas biochemistry entry for CASP3:caspase 3,effector,cysteine containing aspartate-specific protease CPP32,activated by CASP9,cleaving specificallyCDKN1A,alpha-fodrin (SPTAN1), gelsolin (GSN),stored in the mitochondrial intermembrane space and released into cytosolafter appropriate apoptotic stimuli,promoting CD95/APO1/fas mediated apoptosis,homolog to C elegans Ced-3 andmammalian interleukin 1 beta convertase (IL1BC),including two isoforms alpha and beta
Enzyme Numbers (IUBMB): EC 3.4.22.561 2 EC 3.4.222
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CASP3 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CASP3 (see all 4)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): CASP3 (NM_004346) | |  | Sino Biological Human cDNA Clone for CASP3 | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CASP3 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CASP3 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CASP3 |
Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6): About this table
CASP3 for ontologies About GeneDecksing
3 GenomeRNAi human phenotypes for CASP3:
Animal Models:
Mouse knock-outs for CASP3: Casp3tm1Flv Casp3tm1Kin Casp3tm1Mak
15/23 MGI mutant phenotypes (inferred from 6 alleles ) (MGI details for Casp3) (see all 23):
CASP3 for phenotypes About GeneDecksing
|
Pathways & Interactions
for CASP3 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways - 5/78 super-pathways (see all 78) About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | TWEAK Pathway | | | 2 | Apoptosis and survival_Caspase cascade | | | 3 | Apoptotic factor-mediated response | | | 4 | G-protein signaling Regulation of RAC1 activity | | | 5 | Apoptotic Pathways in Synovial Fibroblasts | |
Pathway sources
See GeneCards unified pathways
Show all pathways
5/10 EMD Millipore Pathways for CASP3 (see all 10)
1  R&D Systems Pathway for CASP3
5/43 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for CASP3 (see all 43)
1 
Cell Signaling Technology (CST) Pathway for CASP3
2 Tocris Bioscience Pathways for CASP3
5/10  GeneGo (Thomson Reuters) Pathways for CASP3 (see all 10)
5/28  BioSystems Pathways for CASP3 (see all 28)
5/23 
Reactome Pathways for CASP3 (see all 23)
1  PharmGKB Pathway for CASP3
5/16 
Kegg Pathways (Kegg details for CASP3) (see all 16):
CASP3 for pathways About GeneDecksing
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for CASP3
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
5/238 Interacting proteins for CASP3 (P425741, 2, 3 ENSP000003110324) via UniProtKB, MINT, STRING, and/or I2D (see all 238)| Interactant | Interaction Details |
|---|
| GeneCard | External ID(s) |
|---|
| YWHAE | P622582, 3, ENSP000002643354 | MINT-18746 I2D:
score=4 STRING: ENSP00000264335 | | APAF1 | O147272, 3, ENSP000003530594 | MINT-18036 I2D:
score=3 STRING: ENSP00000353059 | | XIAP | P981702, 3, ENSP000003478584 | MINT-13839 MINT-13838 I2D:
score=7 STRING: ENSP00000347858 | | CASP9 | P552112, 3, ENSP000003302374 | MINT-18815 I2D:
score=4 STRING: ENSP00000330237 | | AKT1 | P317492, 3, ENSP000002702024 | MINT-4820691 I2D:
score=4 STRING: ENSP00000270202 |
About this table
Gene Ontology (GO): 5/33 biological process terms (GO ID links to tree view) (see all 33): About this table
CASP3 for ontologies About GeneDecksing
|
Drugs & Compounds
for CASP3 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
CASP3 for compounds About GeneDecksing
 |
Enzo Life Sciences drugs & compounds for CASP3 |
Compounds for CASP3 available from Tocris Bioscience About this table
| Compound | Action |
CAS
# |
|---|
| Ivachtin | Potent caspase-3 inhibitor | -- | | AZ 10417808 | Selective non-peptide caspase-3 inhibitor | [331645-84-2] | | Z-DQMD-FMK | Caspase-3 inhibitor | -- | | PAC 1 | Activator of procaspase-3; pro-apoptotic | [315183-21-2] | | PETCM | Activator of caspase-3 | [10129-56-3] |
2 HMDB Compounds for CASP3 About this table
10 DrugBank Compounds for CASP3 About this table
| Compound | Synonyms |
CAS
# | Type | Actions | PubMed Ids |
|---|
| Minocycline | -- | 10118-90-8 | target | negative modulator | 15304547 18977395 12112047 10888929 14588118 14568022 | | 5-[4-(1-Carboxymethyl-2-Oxo-Propylcarbamoyl)-Benzylsulfamoyl]-2-Hydroxy-Benzoic Acid | -- | -- | target | -- | 17139284 17016423 10592235 | | (1S)-1-(3-chlorophenyl)-2-oxo-2-[(1,3,4-trioxo-1,2,3,4-tetrahydroisoquinolin-5-yl)amino]ethyl acetate | -- | -- | target | -- | 10592235 | | (1S)-2-oxo-1-phenyl-2-[(1,3,4-trioxo-1,2,3,4-tetrahydroisoquinolin-5-yl)amino]ethyl acetate | -- | -- | target | -- | 10592235 | | 1-METHYL-5-(2-PHENOXYMETHYL-PYRROLIDINE-1-SULFONYL)-1H-INDOLE-2,3-DIONE | -- | -- | target | -- | 10592235 | | 2-HYDROXY-5-(2-MERCAPTO-ETHYLSULFAMOYL)-BENZOIC ACID | -- | -- | target | -- | 10592235 | | 4-[5-(2-CARBOXY-1-FORMYL-ETHYLCARBAMOYL)-PYRIDIN-3-YL]-BENZOIC ACID | -- | -- | target | -- | 10592235 | | N-[3-(2-fluoroethoxy)phenyl]-N'-(1,3,4-trioxo-1,2,3,4-tetrahydroisoquinolin-6-yl)butanediamide | -- | -- | target | -- | 10592235 | | [N-(3-DIBENZYLCARBAMOYL-OXIRANECARBONYL)-HYDRAZINO]-ACETIC ACID | -- | -- | target | -- | 10592235 | | methyl (3S)-3-[(tert-butoxycarbonyl)amino]-4-oxopentanoate | -- | -- | target | -- | 10592235 |
10/130 Novoseek chemical compound relationships for CASP3 gene (see all 130) About this table
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| adpribose |
87.7 |
299 |
11118097 (2), 14966908 (2), 11239917 (2), 19121316 (2) (see all 99) |
| phosphatidylserine |
82.2 |
225 |
18492369 (6), 18441683 (3), 18155705 (3), 18925930 (2) (see all 99) |
| dutp |
81.3 |
79 |
12393527 (1), 15122710 (1), 15517578 (1), 16881058 (1) (see all 71) |
| sp 600125 |
77.4 |
30 |
15130758 (2), 14766793 (1), 16923120 (1), 19248828 (1) (see all 27) |
| 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide |
77 |
21 |
10655438 (1), 14970883 (1), 17850429 (1), 18199664 (1) (see all 15) |
| n acetylcysteine |
76.7 |
101 |
10428064 (2), 17603285 (2), 14726501 (2), 15816534 (2) (see all 76) |
| z-vad-fmk |
73.3 |
10 |
10229803 (1), 16326984 (1), 14999810 (1), 11389070 (1) (see all 9) |
| ly294002 |
73.1 |
42 |
10658194 (2), 20010439 (2), 12606820 (2), 11804874 (1) (see all 35) |
| oxygen |
72.9 |
315 |
10388534 (4), 10998365 (3), 12853978 (3), 16310184 (2) (see all 99) |
| sb 203580 |
72.7 |
42 |
18640003 (2), 10233770 (2), 10026138 (1), 12369731 (1) (see all 34) |
Search CenterWatch for drugs/clinical trials and news about CASP3
|
Transcripts
for CASP3 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for CASP3 gene (2 alternative transcripts): NM_004346.3 NM_032991.2 Unigene Cluster for CASP3: Caspase 3, apoptosis-related cysteine peptidase Hs.141125 [show with all ESTs]Unigene Representative Sequence: NM_0043466 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000308394(uc003iwh.3) ENST00000393585(uc003iwg.3) ENST00000523916(uc003iwi.3)
ENST00000517513 ENST00000393588 ENST00000447121
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CASP3 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CASP3 (see all 4)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): CASP3 (NM_004346) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CASP3 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CASP3 |
Additional cDNA sequence: AJ413269.1 AK291337.1 BC016926.2 BX647609.1 U13737.1 U13738.1 U26943.1 10 DOTS entries: DT.95168006 DT.101983465 DT.454515 DT.100798173 DT.121219812 DT.95167998 DT.97779743 DT.100798171 DT.121219810 DT.91647927 24/231 AceView cDNA sequences (see all 231): BU678741 AI341242 CA397890 AA011446 AI271950 BQ018686 BU619152 AA651804 CD703763 AI374807 Z38379 AA360736 BC016926 AU125557 CA425368 AI536860 AI858799 AI471008 AI079900 AI359475 AI917657 BM797497 U26943 AW089940
GeneLoc Exon Structure
4 Alternative Splicing Database (ASD) splice patterns (SP) for CASP3 About this scheme
| ExUns: | 1a | · | 1b | ^ | 2a | · | 2b | · | 2c | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6a | · | 6b | · | 6c | ^ | 7 | ^ | 8a | · | 8b |
|---|
|
| SP1: | | | | | - | | | | | | | | | | | | | | | | | | | | | | | | |
| SP2: | | | | | - | | - | | - | | | | | | | | | | | | | | | | | | | | |
| SP3: | | | | | - | | | | | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | - | | | | | | | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for CASP3
|
Expression
for CASP3 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| CASP3 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TAATGGTATC
About this image
See CASP3 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for CASP3
SOURCE GeneReport for Unigene cluster: Hs.141125
UniProtKB/Swiss-Prot: CASP3_HUMAN, P42574Tissue specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletalmuscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system
SABiosciences Expression via Pathway-Focused PCR Arrays including CASP3 (see all 20):
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CASP3
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat CASP3 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CASP3 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CASP3 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CASP3 |
Orthologs
for CASP3 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for CASP3 gene from 5/18 species (see all 18) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
CASP31 |
caspase 3, apoptosis-related cysteine peptidase |
70.76(n)
66.91(a) |
|
395476 NM_204725.1 NP_990056.1 |
lizard
(Anolis carolinensis) |
Reptilia |
CASP36 |
-- |
64(a) |
1 ↔ 1 |
5(113294088-113300392) |
African clawed frog
(Xenopus laevis) |
Amphibia |
D89784.12 |
-- |
75.89(n) |
|
D89784.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
casp32 |
caspase 3, apoptosis-related cysteine protease |
74.69(n) |
|
140621 AB047003.1 |
mosquito
(Anopheles gambiae) |
Insecta |
CASPS111 |
AGAP004922-PA |
54.72(n)
42.77(a) |
|
1275726 XM_315012.3 XP_315012.3 |
ENSEMBL Gene Tree for CASP3 (if available)
TreeFam Gene Tree for CASP3 (if available) |
Paralogs
for CASP3 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for CASP3 gene
- CASP162 CASP62 CASP82 CASP122 CASP92 CASP72 CASP22 CASP142
- CASP12 CASP52 CASP102 CASP42
6 SIMAP similar genes for CASP3 using alignment to 3 protein entries: CASP3_HUMAN (see all proteins):DKFZp686N1815 CASP7 CASP6 CASP8 CASP9 CASP2
CASP3 for paralogs About GeneDecksing
1 Pseudogenes.org Pseudogene for CASP3
PGOHUM00000244069
|
Genomic Variants
for CASP3 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
Human Gene Mutation Database (HGMD): CASP3
| SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CASP3 |
|
Disorders
/ Diseases
for CASP3 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database, Novoseek,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
CASP3 for disorders About GeneDecksing
OMIM gene information: 600636
OMIM disorders: --
20/308  diseases for CASP3 (see all 308): About MalaCardsspinal cord injury open-angle glaucoma dentatorubral-pallidoluysian atrophy fanconi anemia, complementation group a
alzheimer's disease sertoli cell-only syndrome spinal cord ischemia spinal muscular atrophy
diffuse large b-cell lymphoma amyotrophic lateral sclerosis human t-cell leukemia virus type 1 primary open angle glaucoma
sudden infant death syndrome lichen planus amyotrophic lateral sclerosis (als) talipes equinovarus
squamous cell carcinoma severe combined immunodeficiency supranuclear palsy dna topoisomerase i
15 diseases from the University of Copenhagen DISEASES database for CASP3:Cancer Vascular disease Toxic encephalopathy Brain disease
Alzheimer's disease Eye disease Heart disease Diabetes mellitus
Huntington's disease Amyotrophic lateral sclerosis Kidney disease Hyperglycemia
Arthritis Liver disease Lung disease 10/93 Novoseek disease relationships for CASP3 gene (see all 93) About this table
| Disease |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| necrosis |
77.4 |
255 |
17185628 (3), 8619857 (2), 11414470 (2), 16111481 (2) (see all 99) |
| tumors |
75.6 |
567 |
15163235 (4), 14627349 (4), 17983518 (4), 12820420 (3) (see all 99) |
| cancer |
75.5 |
147 |
12854132 (7), 10772815 (5), 11593405 (2), 17150164 (2) (see all 99) |
| leukemia |
75.1 |
141 |
9787175 (3), 15192328 (3), 16077919 (3), 17089125 (3) (see all 99) |
| promyelocytic leukemia |
71.1 |
11 |
14555976 (1), 11844698 (1), 15816559 (1), 9489995 (1) (see all 10) |
| colon cancer |
68.4 |
35 |
10855553 (3), 15494216 (2), 17805550 (2), 11894121 (1) (see all 30) |
| neurodegenerative diseases |
65.6 |
5 |
19077216 (1), 19355923 (1), 14702258 (1), 15359610 (1) (see all 5) |
| cell damage |
65.2 |
31 |
14705839 (2), 12198374 (2), 12815112 (1), 16624439 (1) (see all 26) |
| prostate cancer |
60.7 |
41 |
10344723 (2), 11593405 (1), 12168853 (1), 15492266 (1) (see all 36) |
| glioblastoma |
59.4 |
22 |
15072442 (3), 18814044 (3), 15208263 (1), 18164543 (1) (see all 17) |
Human Genome Epidemiology (HuGE) Navigator: CASP3 (40 documents)
Export disorders for CASP3 gene to outside databases
|
Publications
for CASP3 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for CASP3 gene, integrated from 9 sources (see all 5412):
(articles sorted by number of sources associating them with CASP3) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Structure of recombinant human CPP32 in complex with the tetrapeptide acetyl-Asp-Val-Ala-Asp fluoromethyl ketone. (PubMed id 9045680)1, 2, 9 Mittl P.R.E.... Gruetter M.G. (1997)
- CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. (PubMed id 7983002)1, 2, 9 Fernandes-Alnemri T.... Alnemri E.S. (1994)
- Chromosomal localization of the human genes, CPP32, Mch2, Mch3, and Ich-1, involved in cellular apoptosis. (PubMed id 8780721)1, 3, 9 Tiso N....Danieli G.A. (1996)
- In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. (PubMed id 8755496)1, 2, 9 Fernandes-Alnemri T.... Alnemri E.S. (1996)
- Cleavage of huntingtin by apopain, a proapoptotic cysteine protease, is modulated by the polyglutamine tract. (PubMed id 8696339)1, 2, 9 Goldberg Y.P.... Hayden M.R. (1996)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Dual role of BRUCE as an antiapoptotic IAP and a chimeric E2/E3 ubiquitin ligase. (PubMed id 15200957)1, 2 Bartke T....Jentsch S. (2004)
- Potent and selective nonpeptide inhibitors of caspases 3 and 7 inhibit apoptosis and maintain cell functionality. (PubMed id 10821855)1, 2 Lee D.... Nuttall M.E. (2000)
- The three-dimensional structure of apopain/CPP32, a key mediator of apoptosis. (PubMed id 8673606)1, 2 Rotonda J.... Becker J.W. (1996)
- Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. (PubMed id 7596430)1, 2 Nicholson D.W....Miller D.K. (1995)
|
External Searches for CASP3 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing CASP3 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing CASP3 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing CASP3 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for CASP3 gene:
Search GeneIP for patents involving CASP3
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for CASP3 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | OriGene Antibodies for CASP3 | | OriGene shRNA RFP for CASP3 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CASP3 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CASP3 | | | OriGene Protein Over-expression Lysate for CASP3 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for CASP3 | | OriGene 3'-UTR Clone for CASP3 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CASP3 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CASP3 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for CASP3 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for CASP3 | | OriGene Custom Protein Services for CASP3 | | | OriGene Custom Immunoassay Development | | |
| |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat CASP3 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CASP3 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CASP3 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CASP3 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CASP3 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CASP3 |
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| | Antibodies & Assays for CASP3 |
| | | | Tocris compounds for CASP3 |
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 | | | CASP3 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CASP3 |
|  |  |  | | | | ThermoFisher Antibody for CASP3 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat CASP3 |
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