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Aliases for CAPN2 Gene

Aliases for CAPN2 Gene

  • Calpain 2, (M/II) Large Subunit 2 3
  • Calcium-Activated Neutral Proteinase 2 3 4
  • Calpain Large Polypeptide L2 3 4
  • Calpain-2 Large Subunit 3 4
  • Millimolar-Calpain 3 4
  • Calpain M-Type 3 4
  • EC 3.4.22.53 4 64
  • M-Calpain 3 4
  • CANP 2 3 4
  • CANPL2 3 4
  • Calpain 2, Large [Catalytic] Subunit 3
  • Calpain, Large Polypeptide L2 3
  • Calpain-2 Catalytic Subunit 3
  • Calpain 2, Large Subunit 3
  • EC 3.4.22 64
  • CANPml 3
  • CANP2 3
  • MCANP 3

External Ids for CAPN2 Gene

Summaries for CAPN2 Gene

Entrez Gene Summary for CAPN2 Gene

  • The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

GeneCards Summary for CAPN2 Gene

CAPN2 (Calpain 2, (M/II) Large Subunit) is a Protein Coding gene. Among its related pathways are ERK Signaling and Apoptosis Pathway. GO annotations related to this gene include calcium ion binding and cysteine-type peptidase activity. An important paralog of this gene is CAPN9.

UniProtKB/Swiss-Prot for CAPN2 Gene

  • Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at Arg-226 (PubMed:17650508).

Tocris Summary for CAPN2 Gene

  • Calpains are a group of calcium-sensitive cysteine proteases that are ubiquitously expressed in mammals. This family contains 14 members with mu-calpain (calpain 1) and m-calpain (calpain 2) being the most well-characterized. Structurally, calpains contain two subunits; an 80 kDa catalytic subunit and a 28 kDa regulatory subunit that functions as a chaperone to stabilize the 80 kDa structure. Calpains are regulated by Ca2+ concentration, phosphorylation, calpastatin and probably by altering their subcellular localization (limiting access to substrate). These endopeptidases have numerous functions including, but not limited to, remodeling of cytoskeletal attachments to the plasma membrane during cell fusion and cell motility, proteolytic modification of molecules in signal transduction pathways, degradation of enzymes controlling progression through the cell cycle, regulation of gene expression, substrate degradation in some apoptotic pathways, and an involvement in long-term potentiation. Perturbations in calpain activity have been associated in pathophysiological processes contributing to type II diabetes (calpain 10), Alzheimers disease (calpain 1), gastric cancer (calpain 9) and muscular dystrophy (calpain 3).

Gene Wiki entry for CAPN2 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CAPN2 Gene

Genomics for CAPN2 Gene

Genomic Location for CAPN2 Gene

Start:
223,701,593 bp from pter
End:
223,776,018 bp from pter
Size:
74,426 bases
Orientation:
Plus strand

Genomic View for CAPN2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for CAPN2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CAPN2 Gene

Regulatory Elements for CAPN2 Gene

Proteins for CAPN2 Gene

  • Protein details for CAPN2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P17655-CAN2_HUMAN
    Recommended name:
    Calpain-2 catalytic subunit
    Protein Accession:
    P17655
    Secondary Accessions:
    • A6NDG7
    • B7ZA96
    • E7ES58
    • Q16738
    • Q6PJT3
    • Q8WU26
    • Q9HBB1

    Protein attributes for CAPN2 Gene

    Size:
    700 amino acids
    Molecular mass:
    79995 Da
    Cofactor:
    Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 7 Ca(2+) ions.;
    Quaternary structure:
    • Forms a heterodimer with a small (regulatory) subunit (CAPNS1)
    SequenceCaution:
    • Sequence=AAH07686.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305}; Sequence=AAH11828.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for CAPN2 Gene

    Alternative splice isoforms for CAPN2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CAPN2 Gene

Proteomics data for CAPN2 Gene at MOPED

Post-translational modifications for CAPN2 Gene

  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys26, Lys260, Lys476, and Lys674

Other Protein References for CAPN2 Gene

Domains for CAPN2 Gene

Gene Families for CAPN2 Gene

HGNC:
  • EFHAND :EF-hand domain containing

UniProtKB/Swiss-Prot:

CAN2_HUMAN
Domain:
  • Contains 1 calpain catalytic domain.:
    • P17655
  • Contains 3 EF-hand domains.:
    • P17655
Family:
  • Belongs to the peptidase C2 family.:
    • P17655
genes like me logo Genes that share domains with CAPN2: view

Function for CAPN2 Gene

Molecular function for CAPN2 Gene

GENATLAS Biochemistry: calpain,class A,large polypeptide L2,calcium dependent neutral cysteine proteinase,papain superfamily,m type
UniProtKB/Swiss-Prot CatalyticActivity: Broad endopeptidase specificity
UniProtKB/Swiss-Prot EnzymeRegulation: Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin
UniProtKB/Swiss-Prot Function: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at Arg-226 (PubMed:17650508).

Enzyme Numbers (IUBMB) for CAPN2 Gene

Gene Ontology (GO) - Molecular Function for CAPN2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004198 calcium-dependent cysteine-type endopeptidase activity ISS --
GO:0005509 calcium ion binding IEA --
GO:0005515 protein binding IPI 10639123
GO:0008092 cytoskeletal protein binding NAS 12150984
GO:0008233 peptidase activity --
genes like me logo Genes that share ontologies with CAPN2: view
genes like me logo Genes that share phenotypes with CAPN2: view

Animal Models for CAPN2 Gene

MGI Knock Outs for CAPN2:

miRNA for CAPN2 Gene

miRTarBase miRNAs that target CAPN2

No data available for Transcription Factor Targeting and HOMER Transcription for CAPN2 Gene

Localization for CAPN2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CAPN2 Gene

Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for CAPN2 Gene COMPARTMENTS Subcellular localization image for CAPN2 gene
Compartment Confidence
plasma membrane 5
cytosol 3
cytoskeleton 2
endoplasmic reticulum 2
mitochondrion 2
nucleus 2
extracellular 1

Gene Ontology (GO) - Cellular Components for CAPN2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000785 chromatin IEA --
GO:0005622 intracellular --
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm ISS --
GO:0005783 endoplasmic reticulum IDA 14559243
genes like me logo Genes that share ontologies with CAPN2: view

Pathways for CAPN2 Gene

genes like me logo Genes that share pathways with CAPN2: view

Pathways by source for CAPN2 Gene

1 Tocris pathway for CAPN2 Gene
1 GeneGo (Thomson Reuters Life Sciences Research) pathway for CAPN2 Gene

Gene Ontology (GO) - Biological Process for CAPN2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001666 response to hypoxia IEA --
GO:0001824 blastocyst development IEA --
GO:0006508 proteolysis ISS --
GO:0007520 myoblast fusion IEA --
GO:0016540 protein autoprocessing IEA --
genes like me logo Genes that share ontologies with CAPN2: view

Compounds for CAPN2 Gene

(9) ApexBio Compounds for CAPN2 Gene

Compound Action Cas Number
Acetyl-Calpastatin (184-210) (human) Selective calpain inhibitor. [123714-50-1]
Calpain Inhibitor I, ALLN A cell-permeable inhibitor of calpain I, calpain II, cathepsin B, and cathepsin L. [110044-82-1]
Calpain Inhibitor II, ALLM A cell-permeable inhibitor of calpain 1 and calpain 2. [136632-32-1]
Calpeptin Potent cathepsin L inhibitor [117591-20-5]
E-64 Irriversible cysteine protease inhibitor [66701-25-5]
MDL 28170 Potent, selective inhibitor of calpain and cathepsin B. [88191-84-8]
MG-132 Proteasome and calpain inhibitor. [133407-82-6]
PD 150606 Selective, cell-permeable non-peptide calpain inhibitor. [179528-45-1]
Z-FA-FMK An inhibitor of cysteine proteases, such as cathepsin B.

(5) Tocris Compounds for CAPN2 Gene

Compound Action Cas Number
Acetyl-Calpastatin (184-210) (human) Selective calpain inhibitor [123714-50-1]
MDL 28170 Potent, selective calpain and cathepsin B inhibitor [88191-84-8]
MG 101 Calpain inhibitor; activates p53-dependent apoptosis [110044-82-1]
MG 132 Proteasome and calpain inhibitor. Inhibits NF-kappaB activation [133407-82-6]
PD 150606 Cell permeable calpain inhibitor [179528-45-1]

(1) HMDB Compounds for CAPN2 Gene

Compound Synonyms Cas Number PubMed IDs
Calcium
  • Ca
7440-70-2

(24) Novoseek inferred chemical compound relationships for CAPN2 Gene

Compound -log(P) Hits PubMed IDs
calpeptin 84.6 5
pd150606 73.8 1
cysteine 71.1 20
e 64d 57.2 2
calcium 55.9 56
genes like me logo Genes that share compounds with CAPN2: view

Transcripts for CAPN2 Gene

Unigene Clusters for CAPN2 Gene

Calpain 2, (m/II) large subunit:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for CAPN2 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16a · 16b ^ 17a · 17b ^ 18a · 18b · 18c ^ 19a ·
SP1: - - - - -
SP2: - - -
SP3: -
SP4: -
SP5:
SP6:
SP7: - -
SP8:
SP9:
SP10:

ExUns: 19b · 19c ^ 20a · 20b
SP1: - -
SP2: - -
SP3:
SP4:
SP5:
SP6:
SP7:
SP8: - -
SP9:
SP10:

Relevant External Links for CAPN2 Gene

GeneLoc Exon Structure for
CAPN2
ECgene alternative splicing isoforms for
CAPN2

Expression for CAPN2 Gene

mRNA expression in normal human tissues for CAPN2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for CAPN2 Gene

SOURCE GeneReport for Unigene cluster for CAPN2 Gene Hs.350899

mRNA Expression by UniProt/SwissProt for CAPN2 Gene

P17655-CAN2_HUMAN
Tissue specificity: Ubiquitous
genes like me logo Genes that share expressions with CAPN2: view

Orthologs for CAPN2 Gene

This gene was present in the common ancestor of animals.

Orthologs for CAPN2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia CAPN2 36
  • 99.67 (n)
  • 100 (a)
CAPN2 37
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia CAPN2 36
  • 90.38 (n)
  • 94.71 (a)
CAPN2 37
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia CAPN2 36
  • 86.37 (n)
  • 94.04 (a)
CAPN2 37
  • 89 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Capn2 36
  • 87.67 (n)
  • 94 (a)
Capn2 16
Capn2 37
  • 94 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia CAPN2 37
  • 87 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia CAPN2 37
  • 87 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Capn2 36
  • 88.1 (n)
  • 93.86 (a)
chicken
(Gallus gallus)
Aves CAPN2 36
  • 77.95 (n)
  • 81.29 (a)
CAPN2 37
  • 81 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia CAPN2 37
  • 82 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia MGC68474 36
tropical clawed frog
(Silurana tropicalis)
Amphibia capn2.2 36
  • 71.44 (n)
  • 72.96 (a)
Str.4897 36
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.5054 36
zebrafish
(Danio rerio)
Actinopterygii -- 36
CAPN2 (3 of 4) 37
  • 69 (a)
OneToMany
capn2a 37
  • 67 (a)
OneToMany
capn2b 37
  • 65 (a)
OneToMany
capn2l 36
  • 66.1 (n)
  • 69.14 (a)
capn2l 37
  • 69 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta CalpA 38
  • 49 (a)
CalpB 38
  • 44 (a)
CalpC 38
  • 27 (a)
worm
(Caenorhabditis elegans)
Secernentea clp-7 38
  • 45 (a)
clp-6 38
  • 44 (a)
clp-3 38
  • 37 (a)
Species with no ortholog for CAPN2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for CAPN2 Gene

ENSEMBL:
Gene Tree for CAPN2 (if available)
TreeFam:
Gene Tree for CAPN2 (if available)

Paralogs for CAPN2 Gene

Selected SIMAP similar genes for CAPN2 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with CAPN2: view

Variants for CAPN2 Gene

Sequence variations from dbSNP and Humsavar for CAPN2 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type MAF
rs7331 -- 223,775,766(+) CATTA(C/T)AGAAA utr-variant-3-prime
rs10961 -- 223,775,227(-) GAGAG(C/T)GACTC utr-variant-3-prime
rs13341 -- 223,775,565(+) GAACC(A/G)GTATG utr-variant-3-prime
rs17596 -- 223,717,830(+) GCCCT(A/G)GGTAA synonymous-codon, reference
rs17597 -- 223,745,359(+) CCCAC(C/T)AAGGA reference, synonymous-codon

Structural Variations from Database of Genomic Variants (DGV) for CAPN2 Gene

Variant ID Type Subtype PubMed ID
nsv832681 CNV Gain 17160897
esv34082 CNV Loss 18971310
nsv873213 CNV Gain 21882294
nsv437346 CNV Loss 16327808
esv274539 CNV Insertion 20981092
esv271309 CNV Insertion 20981092
nsv520609 CNV Loss 19592680
nsv521758 CNV Loss 19592680
essv335 CNV CNV 17122850
dgv182e1 CNV Complex 17122850
nsv4698 CNV Insertion 18451855

Relevant External Links for CAPN2 Gene

HapMap Linkage Disequilibrium report
CAPN2

Disorders for CAPN2 Gene

(20) Novoseek inferred disease relationships for CAPN2 Gene

Disease -log(P) Hits PubMed IDs
lgmd2a 67.3 1
muscular dystrophies 45.2 1
cataract 30.4 1
muscular dystrophy duchenne 18.2 2
atrophy 14.6 1

Relevant External Links for CAPN2

Genetic Association Database (GAD)
CAPN2
Human Genome Epidemiology (HuGE) Navigator
CAPN2
genes like me logo Genes that share disorders with CAPN2: view

No data available for UniProtKB/Swiss-Prot for CAPN2 Gene

Publications for CAPN2 Gene

  1. The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium. (PMID: 10639123) Strobl S. … Bode W. (Proc. Natl. Acad. Sci. U.S.A. 2000) 3 4 23
  2. Tandemly reiterated negative enhancer-like elements regulate transcription of a human gene for the large subunit of calcium- dependent protease. (PMID: 2539381) Hata A. … Suzuki K. (J. Biol. Chem. 1989) 2 3 4
  3. Molecular cloning of the cDNA for the large subunit of the high-Ca2+- requiring form of human Ca2+-activated neutral protease. (PMID: 2852952) Imajoh S. … Suzuki K. (Biochemistry 1988) 2 3 4
  4. G1-G2 aggrecan product that can be generated by M-calpain on truncation at Ala709-Ala710 is present abundantly in human articular cartilage. (PMID: 17261541) Maehara H. … Shimizu K. (J. Biochem. 2007) 3 23
  5. m-Calpain antagonizes RhoA overactivation and endothelial barrier dysfunction under disturbed shear conditions. (PMID: 19752040) Miyazaki T. … Ohata H. (Cardiovasc. Res. 2010) 3 23

Products for CAPN2 Gene

  • antibodies-online proteins for CAPN2
  • antibodies-online antibodies for CAPN2
  • antibodies-online kits for CAPN2

Sources for CAPN2 Gene

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