CAPN14 Gene
protein-coding GIFtS: 43
GCID: GC02M031370
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calpain 14
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Aliases
for CAPN14 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Calpain 141 2 | | Calcium-Activated Neutral Proteinase 142 3 | | CANP 142 3 | | Calpain-141 | | EC 3.4.22.-3 |
Export aliases for CAPN14 gene to outside databasesPrevious GC identifers: GC02U990305 GC02M031249 GC02M031133 |
Summaries
for CAPN14 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for CAPN14:
Calpains are a family of cytosolic calcium-activated cysteine proteases involved in a variety of cellular processesincluding apoptosis, cell division, modulation of integrin-cytoskeletal interactions, and synaptic plasticity (Dear etal., 2000 (PubMed 10964513)). CAPN14 belongs to the calpain large subunit family.(supplied by OMIM, Mar 2008)
UniProtKB/Swiss-Prot: CAN14_HUMAN, A8MX76Function: Calcium-regulated non-lysosomal thiol-protease (By similarity)
 summary
for CAPN14: Calpains are a group of calcium-sensitive cysteine proteases that are ubiquitously expressed in mammals.
This family contains 14 members with mu-calpain (calpain 1) and m-calpain (calpain 2) being the most
well-characterized. Structurally, calpains contain two subunits; an 80 kDa catalytic subunit and a 28 kDa
regulatory subunit that functions as a chaperone to stabilize the 80 kDa structure. Calpains are regulated
by Ca2+ concentration, phosphorylation, calpastatin and probably by altering their subcellular localization
(limiting access to substrate). These endopeptidases have numerous functions including, but not limited to,
remodeling of cytoskeletal attachments to the plasma membrane during cell fusion and cell motility,
proteolytic modification of molecules in signal transduction pathways, degradation of enzymes controlling
progression through the cell cycle, regulation of gene expression, substrate degradation in some apoptotic
pathways, and an involvement in long-term potentiation. Perturbations in calpain activity have been
associated in pathophysiological processes contributing to type II diabetes (calpain 10), Alzheimer's
disease (calpain 1), gastric cancer (calpain 9) and muscular dystrophy (calpain 3).
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Genomic Views
for CAPN14 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000002.11 NC_018913.1 NT_022184.15
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the CAPN14 gene promoter:
ISGF-3 AML1a SRF (504 AA) Lmo2 STAT3 AP-4 RelA NF-kappaB NF-kappaB1 C/EBPalpha
Other transcription factors
Search SABiosciences Chromatin IP Primers for CAPN14
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CAPN14 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 2p23.1-p21 Ensembl cytogenetic band: 2p23.1 HGNC cytogenetic band: 2p23.1-p21CAPN14 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 2 GeneLoc Exon Structure GeneLoc location for GC02M031370: view genomic region
(about GC identifiers)
Start:
|
31,395,922 bp from pter |
End:
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31,456,724 bp from pter |
Size:
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60,803 bases |
Orientation:
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minus strand |
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Proteins
for CAPN14 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: CAN14_HUMAN, A8MX76 (See
protein sequence)Recommended Name: Calpain-14 Size: 684 amino acids; 79568 Da
Secondary accessions: B3KRU9Alternative splicing: 2 isoforms: A8MX76-1 A8MX76-2 Explore the universe of human proteins at neXtProt for CAPN14: NX_A8MX76
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_A8MX76
CAPN14 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_001138594.1
ENSEMBL proteins: ENSP00000381805 ENSP00000385247 ENSP00000398670
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005622 | intracellular |
IEA | -- |
CAPN14 for ontologies About GeneDecksing
CAPN14 Antibody Products:
Assay Products for CAPN14: |
Protein
Domains /
Families
for CAPN14 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
CAPN14 for domains About GeneDecksing
5/8 InterPro domains/families (see all 8):Graphical View of Domain Structure for InterPro Entry A8MX76ProtoNet protein and cluster: A8MX76 UniProtKB/Swiss-Prot: CAN14_HUMAN, A8MX76Similarity: Belongs to the peptidase C2 familySimilarity: Contains 1 calpain catalytic domainSimilarity: Contains 3 EF-hand domains |
Function
for CAPN14 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: CAN14_HUMAN, A8MX76Function: Calcium-regulated non-lysosomal thiol-protease (By similarity)Enzyme Number (IUBMB): EC 3.4.22.-1
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CAPN14
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CAPN14
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: CAPN14 (NM_001145122) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CAPN14 | |  | Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat CAPN14 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CAPN14 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0004198 | calcium-dependent cysteine-type endopeptidase activity |
IEA | -- | | GO:0005509 | calcium ion binding |
IEA | -- |
CAPN14 for ontologies About GeneDecksing
|
Pathways & Interactions
for CAPN14 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Apoptosis Pathway | |
Pathway sources
See GeneCards unified pathways
Show all pathways
1 Tocris Bioscience Pathway for CAPN14
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for CAPN14
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006508 | proteolysis |
IEA | -- |
CAPN14 for ontologies About GeneDecksing
|
Drugs & Compounds
for CAPN14 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
Compounds for CAPN14 available from Tocris Bioscience About this table
| Compound | Action |
CAS
# |
|---|
| Calpeptin | Calpain and cathepsin L inhibitor | [117591-20-5] | | Acetyl-Calpastatin (184-210) (human) | Selective calpain inhibitor | [123714-50-1] | | MDL 28170 | Potent, selective calpain and cathepsin B inhibitor | [88191-84-8] | | MG 132 | Proteasome and calpain inhibitor. Inhibits NF-kappaB activation | [133407-82-6] | | MG 101 | Calpain inhibitor; activates p53-dependent apoptosis | [110044-82-1] |
1 HMDB Compound for CAPN14 About this table
Search CenterWatch for drugs/clinical trials and news about CAPN14 / CAN14
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Transcripts
for CAPN14 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for CAPN14 gene: NM_001145122.1 Unigene Cluster for CAPN14: Calpain 14 Hs.468059 [show with all ESTs]Unigene Representative Sequence: NM_0011451223 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000398824(uc002rnt.2) ENST00000403897 ENST00000444918(uc010yms.2)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CAPN14
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CAPN14
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: CAPN14 (NM_001145122) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for CAPN14 | | | Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat CAPN14 |
Additional cDNA sequence: AK092257.1 AK296910.1 1 DOTS entry: DT.202850 7 AceView cDNA sequences: AA743820 BX091474 AA883200 AA760673 AK092257 BX476834 AX747435
GeneLoc Exon Structure
|
Expression
for CAPN14 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| CAPN14 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TATTTACAGC
About this image
See CAPN14 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for CAPN14
SOURCE GeneReport for Unigene cluster: Hs.468059
UniProtKB/Swiss-Prot: CAN14_HUMAN, A8MX76Tissue specificity: Not expressed in tissues tested
SABiosciences Custom PCR Arrays for CAPN14
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CAPN14
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse / rat CAPN14 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CAPN14 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CAPN14 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CAPN14 |
Orthologs
for CAPN14 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for CAPN14 gene from 5/13 species (see all 13) About this table
ENSEMBL Gene Tree for CAPN14 (if available)
TreeFam Gene Tree for CAPN14 (if available) |
Paralogs
for CAPN14 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for CAPN14 gene
- CAPN62 CAPN82 CAPN132 CAPN52 CAPN92 CAPN12 CAPN32 CAPN22
- CAPN72 CAPN122 SOLH2 CAPN112 CAPN102
10 SIMAP similar genes for CAPN14 using alignment to 3 protein entries: CAN14_HUMAN (see all proteins):CAPN2 CAPN1 CAPN8 CAPNS1 CAPN11 CAPN9
CAPN3 CAPN13 CAPN12 CAPN5
CAPN14 for paralogs About GeneDecksing
|
Genomic Variants
for CAPN14 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 2 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for CAPN14 (31395922 - 31456724 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for CAPN14
1 CNV: 73153 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CAPN14 |
|
Disorders
/ Diseases
for CAPN14 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
CAPN14 for disorders About GeneDecksing
OMIM gene information: 610229
OMIM disorders: --
6  diseases for CAPN14: About MalaCardsmuscular dystrophy limb-girdle muscular dystrophy gastric cancer alzheimer's disease
neurodegeneration neuronitis
Export disorders for CAPN14 gene to outside databases
|
Publications
for CAPN14 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for CAPN14 gene integrated from 9 sources:
(articles sorted by number of sources associating them with CAPN14) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Identification and characterization of two novel calpain large subunit genes. (PubMed id 11675017)1, 2, 3 Dear T.N. and Boehm T. (2001)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)2 Hillier L.W....Wilson R.K. (2005)
- Gene structure, chromosomal localization, and expression pattern of Capn12, a new member of the calpain large subunit gene family. (PubMed id 10964513)1 Dear T.N....Boehm T. (2000)
|
External Searches for CAPN14 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing CAPN14 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing CAPN14 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing CAPN14 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for CAPN14 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for CAPN14 gene:
Search GeneIP for patents involving CAPN14
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for CAPN14 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for CAPN14 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for CAPN14 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for CAPN14 | | | OriGene Protein Over-expression Lysate for CAPN14 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for CAPN14 | | Browse 3'-UTR reporter clones for miRNA target validation | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for CAPN14 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for CAPN14 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for CAPN14 | | OriGene Custom Protein Services for CAPN14 | | | OriGene Custom Immunoassay Development | | |
| |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat CAPN14 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing CAPN14 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat CAPN14 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat CAPN14 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat CAPN14 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat CAPN14 |
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 | | | CAPN14 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for CAPN14 |
|  |  |  | | | | Search ThermoFisher Antibodies for CAPN14 |
| | | Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat CAPN14 |
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