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CALY Gene

protein-coding   GIFtS: 50

GC10M134989
calcyon neuron-specific vesicular protein
(Previous name: dopamine receptor D1 interacting protein )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: DRD1IP)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
CALCYON 1
DRD1IP 2, 3
NSG3 1, 2
OTTHUMP00000020793 2
Descriptions
D1 dopamine receptor-interacting protein 2
calcyon D1 dopamine receptor-interacting protein (CALCYON) 2
calcyon neuron-specific vesicular protein 2
calcyon protein 2
dopamine receptor D1 interacting protein 1, 2
External Ids
HGNC: 179381
Entrez Gene: 506322
UniProtKB: Q9NYX43
Ensembl: ENSG000001306437
Search outside databases for aliases for CALY gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for CALY:
The protein encoded by this gene is a type II single transmembrane protein. It is required for
maximal stimulated calcium release after stimulation of purinergic or muscarinic but not
beta-adrenergic receptors. The encoded protein interacts with D1 dopamine receptor and may
interact with other DA receptor subtypes and/or GPCRs. [provided by RefSeq]

UniProtKB/Swiss-Prot: CALY_HUMAN, Q9NYX4
Function: Interacts with clathrin light chain A and stimulates clathrin self-assembly and
clathrin-mediated endocytosis

Gene Wiki entry for CALY (DRD1IP)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10q26.3   Ensembl cytogenetic band:  10q26.3   HGNC cytogenetic band: 10q26.3

CALY Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10M134989:     (about GC identifiers)

Start:
134,988,922 bp from pter
End:
135,000,429 bp from pter
Size:
11,508 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000010.9  NT_017795.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: CALY_HUMAN, Q9NYX4 (See protein sequence)
Recommended Name: Neuron-specific vesicular protein calcyon  
Size: 217 amino acids; 23434 Da
Subunit: Interacts with CLTA
Subcellular location: Cytoplasmic vesicle membrane; Single-pass membrane protein. Cell membrane;
Single-pass membrane protein
Caution: Was originally (PubMed:10698743) thought to interact with the D1 dopamine receptor (DRD1)
and to play a role in potentiating calcium ion-dependent signaling but this work was later
retracted
Secondary accessions: Q5VWX3 Q5VWY5 Q5VWY6
Alternative splicing: 3 isoforms:  Q9NYX4-1   Q9NYX4-2   Q9NYX4-3   

Post-translational modifications:

  • Glycosylated (Probable)1


  • REFSEQ proteins: NP_056537.1  

    ENSEMBL proteins: 
    ENSP00000252939 ENSP00000357544 ENSP00000357546 ENSP00000357543 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Calcyon)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886 plasma membrane IEA--
    GO:0005887 integral to plasma membrane IDA14534309
    GO:0030659 cytoplasmic vesicle membrane IEA--
    GO:0031410 cytoplasmic vesicle IDA14534309 16595675
    About this table

    Antibodies for CALY: 
    Browse Antibodies Central at Invitrogen
    Millipore Mono- and Polyclonal Antibodies for the study of CALY
    Sigma-Aldrich Antibodies for CALY
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Calcyon), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for CALY

    Assays for CALY: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR009431 D1-dopamine_rcpt_interact


       GeneDecks  CALY for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9NYX4

    ProtoNet protein and cluster: Q9NYX4

    1 Blocks protein family: IPB009431 D1 dopamine receptor-interacting

    UniProtKB/Swiss-Prot: CALY_HUMAN, Q9NYX4
    Similarity: Belongs to the NSG family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (CALY)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015722

                  Applied Biosystems Silencer® siRNAs for CALY

                  Sigma-Aldrich siRNA for CALY  
                         Sigma-Aldrich shRNA for CALY  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_015722
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_015722
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_015722 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_015722

    UniProtKB/Swiss-Prot: CALY_HUMAN, Q9NYX4
    Function: Interacts with clathrin light chain A and stimulates clathrin self-assembly and
    clathrin-mediated endocytosis

    2 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Caly):

    nervous systemnormal

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0032051 clathrin light chain binding IDA16595675
    GO:0050780 dopamine receptor binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  CALY  (Your Favorite Gene powered by Ingenuity) 
     Dopamine Receptor Signaling

       GeneDecks  CALY for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  CALY 


    3 Interacting proteins for CALY (ENSP000002529393) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DRD1ENSP000003276523STRING (score=.969)
    CLTCENSP000002691223STRING (score=.754)
    CLTCL1ENSP000002632003STRING (score=.754)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007212 dopamine receptor signaling pathway IEA--
    GO:0045807 positive regulation of endocytosis IMP16595675
    GO:0048268 clathrin coat assembly IDA16595675
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for CALY
    1 Novoseek chemical compound relationship for CALY gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    calcium 13.31 1 16172615 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (CALY)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015722

                  Sigma-Aldrich siRNA for CALY  
                         Sigma-Aldrich shRNA for CALY  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_015722  

    REFSEQ mRNAs for CALY gene: 

    NM_015722.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_015722  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_015722
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_015722
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_015722 

    Additional cDNA sequence: 

    AF225903.1 BC038978.1 CR606939.1 

    5 DOTS entries:

    DT.435605  DT.97861824  DT.75132223  DT.95152987  DT.95145204 

    24/77 AceView cDNA sequences (see all 77 ):

    BQ776602 BM311252 R89656 AI475073 CR606939 BM695916 BM053073 AI203942 
    BC038978 BM696026 BQ787629 BI757112 AI197769 BM503261 BM673540 BM713360 
    CD107799 BI822293 AW137855 BQ776918 BM311840 BM054860 BM728148 AF225903 

    Unigene Cluster for CALY:

    Calcyon neuron-specific vesicular protein
    Hs.148680  [show with all ESTs]
    Unigene Representative Sequence: BM808864


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for CALY

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7
    SP1:                                      -           -     -                     
    SP2:                                      -                                       
    SP3:                                      -                                       
    SP4:                                                                              
    SP5:                                                                              

    About this scheme

    ECgene alternative splicing isoforms for CALY

    4 Ensembl transcripts including schematic representations:
    ENST00000252939  ENST00000368556  ENST00000368558  ENST00000368555  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    CALY expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for CALY

    1 / 2

    3 probe-sets matching CALY gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    77176_at2 U95-E 2 0.88 0.96 1.00 1.00 -- -- -- -- --

    219896_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    219896_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  CALY for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TACAAGGCCA

    SOURCE GeneReport for Unigene cluster: Hs.148680

    UniProtKB/Swiss-Prot: CALY_HUMAN, Q9NYX4
    Tissue specificity: Expressed in the pyramidal cells of the prefrontal cortex, in hippothalamus and
    in caudate nucleus. No expression in spleen. Up-regulated in the prefrontal cortex of
    schizophrenic patients with nearly twice the levels of non-schizophrenics

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for CALY gene from 5 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    CALY1   -- calcyon neuron-specific vesicular protein 88.99(n)
    89.77(a)
    491697  XM_548818.2  XP_548818.2 
    chimpanzee
    (Pan troglodytes)
    CALY1   -- calcyon neuron-specific vesicular protein 99.23(n)
    99.08(a)
    450851  XM_508133.2  XP_508133.2 
    cow
    (Bos taurus)
    CALY1   -- calcyon neuron-specific vesicular protein 81.36(n)
    71.09(a)
    531593  NM_001075953.1  NP_001069421.1 
    rat
    (Rattus norvegicus)
    Caly1   -- calcyon neuron-specific vesicular protein 82.48(n)
    82.33(a)
    192349  NM_138915.1  NP_620270.1 
    mouse
    (Mus musculus)
    Caly1, 5 75
    calcyon neuron-specific vesicular protein1, 5 81.71(n)1
    81.86(a)1
    685661  NM_026769.31  NP_081045.21 
     AK0029795  AK0034005  (see all 8)
    About this table        Species with no ortholog for CALY

    ENSEMBL Gene Tree for CALY
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for CALY gene
    HMP192  D4S234E2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/64 NCBI SNPs in CALY are shown (see all 64 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 18)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 10 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs22981221,2
    A,C,F,H134991562(+) CAAGAT/GAGCCC 1 -- int113Minor allele frequency- G:0.19EA EU WA NA 2674
    rs22757231,2
    A,C,F134989625(+) GGAGGG/AGCCGG 1 -- int1 ese31Minor allele frequency- A:0.10EA 1500
    rs111016941,2
    C,F,H134996704(+) CTGCGT/CGGAGG 1 -- int14Minor allele frequency- C:0.19EU EA WA 418
    rs74759051,2
    F,H134990001(+) GATTTA/TAAAAA 1 -- int1 trp34Minor allele frequency- T:0.03EU EA WA 416
    --
    rs108577021,2
    A,C134995377(+) CAACAA/CTTTGG 1 -- int10--------
    --
    rs107452931,2
    C134995811(+) GAGCAA/GTGAGC 1 -- int10--------
    --
    rs108577011,2
    A134995062(+) AGCTGA/TGGAGC 1 -- int10--------
    --
    rs362130361,2
    --135001478(-) TTTAG-/CATGTA 1 -- ng310--------
    --
    rs357823301,2
    --134989315(-) CGTCT-/CCCAGC 1 -- ut310--------
    --
    rs73929891,2
    --135000852(+) acacaA/Ccacac 1 -- ng310--------
    About this table

    HapMap Linkage Disequilibrium images for CALY (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 604647

    2 Novoseek disease relationships for CALY gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    calcyon 98.58 6 16172615 (2), 12622665 (2), 14755439 (1), 10698743 (1)
    schizophrenia 35.49 2 12622665 (2)
    About this table

    Human Genome Epidemiology Navigator: CALY (0 documents)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/15 PubMed articles for CALY gene (see all 15 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 50632 HGNC: 17938 AceView: DRD1IP Ensembl:ENSG00000130643 euGenes: HUgn50632
    ECgene: CALY H-InvDB: CALY
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for CALY:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for CALY-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for CALY Antibodies for CALY
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for CALY Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Search Novus for antibodies for CALY

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