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C8orf34 Gene

protein-coding   GIFtS: 40
GCID: GC08P069242

Chromosome 8 Open Reading Frame 34

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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Chromosome 8 Open Reading Frame 341 2     VEST12
Vestibule 11 2     Uncharacterized Protein C8orf342
Protein VEST-12 3     Vestibule-1 Protein2
VEST-12     

External Ids:    HGNC: 309051   Entrez Gene: 1163282   Ensembl: ENSG000001650847   UniProtKB: Q49A923   

Export aliases for C8orf34 gene to outside databases

Previous GC identifers: GC08P069406 GC08P064842


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for C8orf34 Gene:
C8orf34 (chromosome 8 open reading frame 34) is a protein-coding gene. GO annotations related to this gene include cAMP-dependent protein kinase regulator activity.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000008.10  NT_008183.20  NC_018919.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the C8orf34 gene promoter:
         USF1   SREBP-1c   MyoD   Tal-1beta   E47   Arnt   SREBP-1b   SREBP-1a   Zic1   RSRFC4   
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search Chromatin IP Primers for C8orf34

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat C8orf34


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8q13   Ensembl cytogenetic band:  8q13.2   HGNC cytogenetic band: 8q13

C8orf34 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
C8orf34 gene location

GeneLoc information about chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08P069242:  view genomic region     (about GC identifiers)

Start:
69,242,957 bp from pter      End:
69,731,258 bp from pter
Size:
488,302 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: CH034_HUMAN, Q49A92 (See protein sequence)
Recommended Name: Uncharacterized protein C8orf34  
Size: 452 amino acids; 50489 Da
Sequence caution: Sequence=AAH41961.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
Sequence=AAH41961.1; Type=Frameshift; Positions=429;
Secondary accessions: A8K5X1 G3XAM6 Q8N1X0 Q8N9M7 Q8ND19 Q96Q28
Alternative splicing: 6 isoforms:  Q49A92-1   Q49A92-2   Q49A92-3   Q49A92-4   Q49A92-5   Q49A92-6   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for C8orf34: NX_Q49A92

Explore proteomics data for C8orf34 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See C8orf34 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001182568.1  NP_443190.2  

    ENSEMBL proteins: 
     ENSP00000427820   ENSP00000429102   ENSP00000345255   ENSP00000337174   ENSP00000429081  
     ENSP00000319532   ENSP00000438159  

    C8orf34 Human Recombinant Protein Products:

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    OriGene Custom MassSpec
    OriGene Custom Protein Services for C8orf34
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    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

     
    Search eBioscience for Proteins for C8orf34 

    C8orf34 Antibody Products:

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    LSBio Antibodies in human, mouse, rat for C8orf34

    C8orf34 Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.
    Search eBioscience for ELISAs for C8orf34 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    1 InterPro protein domain:
     IPR003117 cAMP_dep_PK_reg_su_I/II_a/b

    Graphical View of Domain Structure for InterPro Entry Q49A92

    ProtoNet protein and cluster: Q49A92


    Find genes that share domains with C8orf34           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Phenotypes:
         1 GenomeRNAi human phenotype for C8orf34:
     Increased gamma-H2AX phosphory 

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for C8orf34
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       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for C8orf34
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for C8orf34

    miRNA
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    Block miRNA regulation of human, mouse, rat C8orf34 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate C8orf34 (see all 17):
    hsa-miR-142-5p hsa-miR-548j hsa-miR-548i hsa-miR-340 hsa-miR-891b hsa-miR-3662 hsa-miR-548d-5p hsa-miR-548a-5p
    SwitchGear 3'UTR luciferase reporter plasmidC8orf34 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for C8orf34
    Predesigned siRNA for gene silencing in human, mouse, rat C8orf34

    Gene Editing
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    DNA2.0 Custom Protein Engineering Service for C8orf34

    Clone
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    OriGene clones in human, mouse for C8orf34 (see all 12)
    OriGene ORF clones in mouse, rat for C8orf34
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): C8orf34 (NM_052958)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for C8orf34
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat C8orf34

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for C8orf34 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for C8orf34


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus3
    cytosol1

    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for C8orf34
    Interactions:

        Search GeneGlobe Interaction Network for C8orf34

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for C8orf34 (CH034)

    1 PharmGKB related drug/compound annotation for C8orf34 gene    About this table
    Drug/compound PharmGKB Annotation
    irinotecanCA  



    Find genes that share compounds with C8orf34           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for C8orf34 gene (2 alternative transcripts): 
    NM_001195639.1  NM_052958.2  

    Unigene Cluster for C8orf34:

    Chromosome 8 open reading frame 34
    Hs.491941  [show with all ESTs]
    Unigene Representative Sequence: NM_001195639
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000518698(uc010lyz.3) ENST00000349492 ENST00000523686(uc010lyx.2)
    ENST00000348340(uc010lyy.2) ENST00000337103(uc003xyb.3) ENST00000521406
    ENST00000518515 ENST00000325233 ENST00000539993
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat C8orf34 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate C8orf34 (see all 17):
    hsa-miR-142-5p hsa-miR-548j hsa-miR-548i hsa-miR-340 hsa-miR-891b hsa-miR-3662 hsa-miR-548d-5p hsa-miR-548a-5p
    SwitchGear 3'UTR luciferase reporter plasmidC8orf34 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for C8orf34
    Predesigned siRNA for gene silencing in human, mouse, rat C8orf34
    Clone
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    OriGene ORF clones in mouse, rat for C8orf34
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): C8orf34 (NM_052958)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for C8orf34
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat C8orf34
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for C8orf34
    OriGene qSTAR qPCR primer pairs in human, mouse for C8orf34
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat C8orf34
      QuantiTect SYBR Green Assays in human, mouse, rat C8orf34
      QuantiFast Probe-based Assays in human, mouse, rat C8orf34

    Additional mRNA sequence: 

    AB056652.1 AK094650.1 AK291436.1 AL834454.1 BC041961.1 

    8 DOTS entries:

    DT.308056  DT.95264002  DT.100731581  DT.75151443  DT.120632780  DT.91691929  DT.92019881  DT.75129924 

    Selected AceView cDNA sequences (see all 34):

    BM930891 F12291 AK094191 AB077325 T16367 AL834454 Z42198 AB056652 
    BI753762 BM684412 F09916 AI445354 NM_052958 Z38451 AL565344 BX464780 
    AK094650 BF478251 BC041961 BE504044 BI597942 BI669066 BI597413 AI167925 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    C8orf34 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: ATTATTTTCT
    C8orf34 Expression
    About this image

    C8orf34 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    C8orf34 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.491941
        Custom PCR Arrays for C8orf34
    Primer
    Products:
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    QuantiFast Probe-based Assays in human, mouse, rat C8orf34
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for C8orf34

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of chordates.

    Orthologs for C8orf34 gene from Selected species (see all 11)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia A830018L16Rik1 , 5 RIKEN cDNA A830018L16 gene1, 5 87.7(n)1
    91.11(a)1
      1 (2.57 cM)5
    3204921  NM_001160369.21  NP_001153841.11 
     114141055 
    chicken
    (Gallus gallus)
    Aves C8ORF341 chromosome 2 open reading frame, human C8orf34 79.63(n)
    81.73(a)
      768864  XM_001232048.3  XP_001232049.3 
    lizard
    (Anolis carolinensis)
    Reptilia C8orf346
    chromosome 8 open reading frame 34
    76(a)
    1 ↔ 1
    4(31577860-31677473)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia LOC1004922241 chromosome unknown open reading frame, human C8orf34 75.77(n)
    77.43(a)
      100492224  XM_002935323.2  XP_002935369.2 
    zebrafish
    (Danio rerio)
    Actinopterygii zgc:1629281 zgc:162928 70.58(n)
    71.47(a)
      557144  NM_001089499.1  NP_001082968.1 


    ENSEMBL Gene Tree for C8orf34 (if available)
    TreeFam Gene Tree for C8orf34 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for C8orf34 (see all 9562)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 8 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0426904
    A colorectal cancer sample4--see VAR_0426902 K N mis40--------
    rs1998192001,2
    Cuntested169400281(+) AACTCA/GATAAG 4 N D mis10--------
    rs78181471,2
    C,F,A,H--69245872(+) AGACCG/ATCCAT 2 -- int110Minor allele frequency- A:0.39NA WA CSA EA 372
    rs1412633441,2
    --69245897(+) TTACTA/CAATAG 2 -- int10--------
    rs1441531291,2
    C--69245919(+) ATTGA-/TTTGGG 2 -- int10--------
    rs1507821421,2
    --69245955(+) ACATAC/TTTAGA 2 -- int10--------
    rs1914625921,2
    --69246063(+) CTTCAC/TGTCTT 2 -- int10--------
    rs1123919431,2
    C--69246134(+) AAATAC/TGCGGT 2 -- int11Minor allele frequency- T:0.50CSA 2
    rs1837181101,2
    --69246387(+) CTTATC/TAAAGT 2 -- int10--------
    rs69910821,2
    C,F,A,H--69246455(+) AGCTGA/TCTCAG 2 -- int19Minor allele frequency- T:0.21NS EA WA CSA NA 661

    HapMap Linkage Disequilibrium report for C8orf34 (69242957 - 69492957 bp, first 250kb of C8orf34)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for C8orf34 (see all 25):    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2670093CNV Deletion23128226
    esv2088334CNV Deletion18987734
    esv2587232CNV Deletion19546169
    esv2178446CNV Deletion18987734
    esv2737129CNV Deletion23290073
    esv2737130CNV Deletion23290073
    esv2737127CNV Deletion23290073
    esv2664364CNV Deletion23128226
    esv269339CNV Insertion20981092
    nsv508512CNV Loss20534489

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing C8orf34
    DNA2.0 Custom Variant and Variant Library Synthesis for C8orf34

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    Find genes that share disorders with C8orf34           About GenesLikeMe

    Genetic Association Database (GAD): C8orf34

    Export disorders for C8orf34 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for C8orf34 gene, integrated from 10 sources (see all 13):
    (articles sorted by number of sources associating them with C8orf34)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    2. Why do cellular proteins linked to K63-polyubiquitin chains not associate with proteasomes? (PubMed id 23314748)1 Nathan J.A....Goldberg A.L. (EMBO J. 2013)
    3. A genome-wide association study for irinotecan-related severe toxicities in patients with advanced non-small-cell lung cancer. (PubMed id 22664479)1 Han J.Y....Lee J.S. (Pharmacogenomics J. 2013)
    4. Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population. (PubMed id 23251661)1 Comuzzie A.G....Butte N.F. (PLoS ONE 2012)
    5. Wnt signaling and Dupuytren's disease. (PubMed id 21732829)1 Dolmans G.H.... . (N. Engl. J. Med. 2011)
    6. Framingham Heart Study 100K Project: genome-wide associations for blood pressure and arterial stiffness. (PubMed id 17903302)4 Levy D....Mitchell G.F. (BMC Med. Genet. 2007)
    7. The full-ORF clone resource of the German cDNA consortium. (PubMed id 17974005)2 Bechtel S.... Schupp I. (BMC Genomics 2007)
    8. The consensus coding sequences of human breast and colorectal cancers. (PubMed id 16959974)2 Sjoeblom T.... Velculescu V.E. (Science 2006)
    9. DNA sequence and analysis of human chromosome 8. (PubMed id 16421571)2 Nusbaum C....Lander E.S. (Nature 2006)
    10. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S.... Malek J. (Genome Res. 2004)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 116328 HGNC: 30905 AceView: VEST1 Ensembl:ENSG00000165084 euGenes: HUgn116328
    ECgene: C8orf34 H-InvDB: C8orf34

    (According to HUGE)
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for C8orf34 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for C8orf34 gene:
    Search GeneIP for patents involving C8orf34

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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