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Aliases for C14orf169 Gene

Aliases for C14orf169 Gene

  • Chromosome 14 Open Reading Frame 169 2 3
  • Nucleolar Protein 66 2 3 4
  • MAPJD 3 4 6
  • 60S Ribosomal Protein L8 Histidine Hydroxylase 3 4
  • Myc-Associated Protein With JmjC Domain 2 4
  • Histone Lysine Demethylase NO66 3 4
  • Ribosomal Oxygenase NO66 3 4
  • HsNO66 3 4
  • NO66 3 6
  • ROX 3 4
  • Bifunctional Lysine-Specific Demethylase And Histidyl-Hydroxylase NO66 3
  • MYC-Associated Protein With JmjC Domain 3
  • Lysine-Specific Demethylase NO66 3
  • Up-Regulated In Lung Cancer 2 3
  • EC 1.14.11.27 4
  • EC 1.14.11.- 4
  • URLC2 3

External Ids for C14orf169 Gene

Previous GeneCards Identifiers for C14orf169 Gene

  • GC14P071948
  • GC14P071949
  • GC14P073027
  • GC14P073958
  • GC14P054124

Summaries for C14orf169 Gene

GeneCards Summary for C14orf169 Gene

C14orf169 (Chromosome 14 Open Reading Frame 169) is a Protein Coding gene. GO annotations related to this gene include iron ion binding and histone demethylase activity (H3-K36 specific).

UniProtKB/Swiss-Prot for C14orf169 Gene

  • Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates Lys-4 (H3K4me) and Lys-36 (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 Lys-4 (H3K4me3) and monomethylated H3 Lys-4 (H3K4me1) residues, while it has weaker activity for dimethylated H3 Lys-36 (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on His-216. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for C14orf169 Gene

Genomics for C14orf169 Gene

Regulatory Elements for C14orf169 Gene

Genomic Location for C14orf169 Gene

Start:
73,490,939 bp from pter
End:
73,493,401 bp from pter
Size:
2,463 bases
Orientation:
Plus strand

Genomic View for C14orf169 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for C14orf169 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for C14orf169 Gene

Proteins for C14orf169 Gene

  • Protein details for C14orf169 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H6W3-NO66_HUMAN
    Recommended name:
    Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
    Protein Accession:
    Q9H6W3
    Secondary Accessions:
    • B4DT02

    Protein attributes for C14orf169 Gene

    Size:
    641 amino acids
    Molecular mass:
    71086 Da
    Cofactor:
    Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 1 Fe(2+) ion per subunit.;
    Quaternary structure:
    • Interacts with SP7/OSX; the interaction is direct (By similarity). Interacts with MYC. Interacts with PHF19; leading to its recruitment to H3K36me3 sites.

    Three dimensional structures from OCA and Proteopedia for C14orf169 Gene

    Alternative splice isoforms for C14orf169 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for C14orf169 Gene

Proteomics data for C14orf169 Gene at MOPED

Post-translational modifications for C14orf169 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for C14orf169 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

No data available for DME Specific Peptides for C14orf169 Gene

Domains for C14orf169 Gene

Protein Domains for C14orf169 Gene

Graphical View of Domain Structure for InterPro Entry

Q9H6W3

UniProtKB/Swiss-Prot:

NO66_HUMAN :
  • Q9H6W3
Domain:
  • Contains 1 JmjC domain.
Family:
  • Belongs to the MINA53/NO66 family. NO66 subfamily.
genes like me logo Genes that share domains with C14orf169: view

No data available for Gene Families and Suggested Antigen Peptide Sequences for C14orf169 Gene

Function for C14orf169 Gene

Molecular function for C14orf169 Gene

UniProtKB/Swiss-Prot CatalyticActivity: Protein N(6),N(6)-dimethyl-L-lysine + 2-oxoglutarate + O(2) = protein N(6)-methyl-L-lysine + succinate + formaldehyde + CO(2).
UniProtKB/Swiss-Prot CatalyticActivity: Protein N(6)-methyl-L-lysine + 2-oxoglutarate + O(2) = protein L-lysine + succinate + formaldehyde + CO(2).
UniProtKB/Swiss-Prot CatalyticActivity: L-histidine-[60S ribosomal protein L8] + 2-oxoglutarate + O(2) = (3S)-3-hydroxy-L-histidine-[60S ribosomal protein L8] + succinate + CO(2).
UniProtKB/Swiss-Prot Function: Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates Lys-4 (H3K4me) and Lys-36 (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 Lys-4 (H3K4me3) and monomethylated H3 Lys-4 (H3K4me1) residues, while it has weaker activity for dimethylated H3 Lys-36 (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on His-216. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.

Enzyme Numbers (IUBMB) for C14orf169 Gene

Gene Ontology (GO) - Molecular Function for C14orf169 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005506 iron ion binding ISS --
GO:0005515 protein binding IPI 19927124
GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors ISS --
GO:0032453 histone demethylase activity (H3-K4 specific) ISS --
GO:0051864 histone demethylase activity (H3-K36 specific) ISS --
genes like me logo Genes that share ontologies with C14orf169: view

Phenotypes for C14orf169 Gene

GenomeRNAi human phenotypes for C14orf169:
genes like me logo Genes that share phenotypes with C14orf169: view

Animal Model Products

CRISPR Products

No data available for Animal Models , miRNA , Transcription Factor Targeting and HOMER Transcription for C14orf169 Gene

Localization for C14orf169 Gene

Subcellular locations from UniProtKB/Swiss-Prot for C14orf169 Gene

Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Granular part of nucleoli. Nucleoplasm, nucleoplasmic foci, some of them associated with nucleoli.

Gene Ontology (GO) - Cellular Components for C14orf169 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus ISS --
GO:0005654 nucleoplasm IEA --
GO:0005730 nucleolus IEA --
genes like me logo Genes that share ontologies with C14orf169: view

No data available for Subcellular locations from COMPARTMENTS for C14orf169 Gene

Pathways for C14orf169 Gene

SuperPathways for C14orf169 Gene

No Data Available

Interacting Proteins for C14orf169 Gene

Gene Ontology (GO) - Biological Process for C14orf169 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated IEA --
GO:0034720 histone H3-K4 demethylation ISS --
GO:0045668 negative regulation of osteoblast differentiation ISS --
GO:0045892 negative regulation of transcription, DNA-templated ISS --
GO:0055114 oxidation-reduction process IEA --
genes like me logo Genes that share ontologies with C14orf169: view

No data available for Pathways by source for C14orf169 Gene

Transcripts for C14orf169 Gene

mRNA/cDNA for C14orf169 Gene

(57) Selected AceView cDNA sequences:
(5) Additional mRNA sequences :
(1) REFSEQ mRNAs :
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for C14orf169 Gene

Chromosome 14 open reading frame 169:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for C14orf169

Primer Products

  • QuantiTect SYBR Green Assays in human,mouse,rat
  • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
  • QuantiFast Probe-based Assays in human,mouse,rat

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for C14orf169 Gene

No ASD Table

Relevant External Links for C14orf169 Gene

GeneLoc Exon Structure for
C14orf169
ECgene alternative splicing isoforms for
C14orf169

Expression for C14orf169 Gene

mRNA expression in normal human tissues for C14orf169 Gene

Integrated Proteomics: protein expression from ProteomicsDB, MOPED, and MaxQB for C14orf169 Gene

SOURCE GeneReport for Unigene cluster for C14orf169 Gene Hs.509916

mRNA Expression by UniProt/SwissProt for C14orf169 Gene

Q9H6W3-NO66_HUMAN
Tissue specificity: Widely expressed. Overexpressed in lung carcinomas.
genes like me logo Genes that share expressions with C14orf169: view

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for C14orf169 Gene

Orthologs for C14orf169 Gene

This gene was present in the common ancestor of animals.

Orthologs for C14orf169 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia NO66 35
  • 87.47 (n)
  • 90.25 (a)
dog
(Canis familiaris)
Mammalia C8H14orf169 35
  • 89.83 (n)
  • 93.87 (a)
mouse
(Mus musculus)
Mammalia 2410016O06Rik 35
  • 81.91 (n)
  • 83.25 (a)
2410016O06Rik 16
rat
(Rattus norvegicus)
Mammalia RGD1307704 35
  • 82.94 (n)
  • 85.03 (a)
chicken
(Gallus gallus)
Aves C5H14ORF169 35
  • 64.03 (n)
  • 60.08 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100488246 35
  • 59.12 (n)
  • 54.29 (a)
zebrafish
(Danio rerio)
Actinopterygii Dr.4334 35
zgc:162967 35
  • 56.92 (n)
  • 52.69 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP001938 35
  • 54.17 (n)
  • 45.76 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG2982 35
  • 51.89 (n)
  • 41.76 (a)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.4160 35
Species with no ortholog for C14orf169:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chimpanzee (Pan troglodytes)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for C14orf169 Gene

ENSEMBL:
Gene Tree for C14orf169 (if available)
TreeFam:
Gene Tree for C14orf169 (if available)

Paralogs for C14orf169 Gene

No data available for Paralogs for C14orf169 Gene

Variants for C14orf169 Gene

Structural Variations from Database of Genomic Variants (DGV) for C14orf169 Gene

Variant ID Type Subtype PubMed ID
dgv1958n71 CNV Gain 21882294
nsv510643 CNV Loss 20534489

Relevant External Links for C14orf169 Gene

HapMap Linkage Disequilibrium report
C14orf169

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations from dbSNP and Humsavar for C14orf169 Gene

Disorders for C14orf169 Gene

No disorders were found for C14orf169 Gene.

No data available for UniProtKB/Swiss-Prot for C14orf169 Gene

Publications for C14orf169 Gene

  1. Identification of Myc-associated protein with JmjC domain as a novel therapeutic target oncogene for lung cancer. (PMID: 17308053) Suzuki C. … Daigo Y. (Mol. Cancer Ther. 2007) 2 3 4
  2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4
  3. NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin. (PMID: 14742713) Eilbracht J. … Schmidt-Zachmann M.S. (Mol. Biol. Cell 2004) 3 4
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4
  5. Regulation of the osteoblast-specific transcription factor Osterix by NO66, a Jumonji family histone demethylase. (PMID: 19927124) Sinha K.M. … de Crombrugghe B. (EMBO J. 2010) 2 3

Products for C14orf169 Gene

Sources for C14orf169 Gene

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