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WDFY4 Gene

protein-coding GIFtS: 35

GC10P049563

WDFY family member 4
(Previous name: chromosome 10 open reading frame 64 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: C10orf64)

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
C10orf64 ^², ³
Em:AC060234.3 ^¹
FLJ17416 ^²
FLJ36288 ^²
FLJ45748 ^¹, ²
KIAA1607 ^¹, ², ³
MGC40604 ^²
MGC45899 ^²

Descriptions
WDFY family member 4 ^²
chromosome 10 open reading frame 64 ^¹

External Ids
HGNC: 29323¹
Entrez Gene: 57705²
UniProtKB: Q6ZS81³
Ensembl: ENSG00000128815⁷

Search outside databases for aliases for WDFY4 gene

Previous GC identifers: GC10P049313 GC10U900444

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10q11.22 Ensembl cytogenetic band: 10q11.22 HGNC cytogenetic band: 10q11.23

WDFY4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10 GeneLoc Exon Structure

GeneLoc location for GC10P049563: (about GC identifiers)

Start:	49,562,927 bp from pter
End:	49,861,006 bp from pter
Size:	298,080 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000010.9 NT_017696.14

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: WDFY4_HUMAN, Q6ZS81 (See protein sequence)

Recommended Name: Protein WDFY4

Size: 3184 amino acids; 353610 Da

Subcellular location: Membrane; Multi-pass membrane protein (Potential)

Secondary accessions: Q86WZ4 Q8N4A3 Q8TEN7 Q96BE1 Q9H7H8 Q9HCG5

Alternative splicing: 5 isoforms: Q6ZS81-1 Q6ZS81-2 Q6ZS81-3 Q6ZS81-4 Q6ZS81-5

REFSEQ proteins: NP_065996.1

ENSEMBL proteins:

ENSP00000354141 ENSP00000320563 ENSP00000309314 ENSP00000363276 ENSP00000265453

Human Recombinant Proteins

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Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0016020	membrane	IEA	--
GO:0016021	integral to membrane	IEA	--

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Antibodies for WDFY4:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
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	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for WDFY4

Assays for WDFY4:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry Q6ZS81

ProtoNet protein and cluster: Q6ZS81

UniProtKB/Swiss-Prot: WDFY4_HUMAN, Q6ZS81

Similarity: Contains 1 BEACH domain

Similarity: Contains 5 WD repeats

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Browse Invitrogen for RNAi
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

Browse Origene for 29mer shRNA kit in GFP-retroviral vector

Applied Biosystems Silencer® siRNAs for WDFY4

Browse siRNA at Sigma-Aldrich

Browse shRNA at Sigma-Aldrich

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_020945
Myc/DDK tagged cDNA clone in CMV expression vector: NM_020945
untagged cDNA clones in CMV expression vector (see all 2): BC034937

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_020945

1 Gene Ontology (GO) molecular function term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005488	binding	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Interacting protein for WDFY4 (ENSP00000309314³) via UniProtKB, MINT, and/or STRING

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
FRMPD2	ENSP00000363317³	STRING (score=.712)

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for WDFY4

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Browse Invitrogen for RNAi
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

Browse Origene for 29mer shRNA kit in GFP-retroviral vector

Browse siRNA at Sigma-Aldrich

Browse shRNA at Sigma-Aldrich

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_020945

REFSEQ mRNAs for WDFY4 gene:

NM_020945.1

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_020945

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_020945
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_020945
                                 untagged cDNA clones in CMV expression vector (see all 2): BC034937

Additional cDNA sequence:

AB046827.1 AK074085.1 AK074443.1 AK093607.1 AK127650.1 AK301736.1 AL833669.1 BC015694.2

BC032420.1 BC034937.1 BC047574.1 CR610316.1

11 DOTS entries:

DT.207837 DT.86853800 DT.100746825 DT.99928057 DT.91862719 DT.101958248 DT.97803385 DT.100865949

DT.121230791 DT.40311322 DT.97814933

highest scoring ESTs for WDFY4:

AA236772 AA372281 AA677699 AA689297 AA835894 AI475727 AI590533 AI703224 AI769422 AI819490

Unigene Cluster for WDFY4:

WDFY family member 4

Hs.287379 [show with all ESTs]

Unigene Representative Sequence: NM_020945

GeneLoc Exon Structure

5/12 Alternative Splicing Database (ASD) splice patterns (SP) for WDFY4 (see all 12 )

ExUns:	1	^	2	^	3	^	4	^	5	^	6	^	7	^	8	^	9	^	10	^	11	^	12a	·	12b	^	13	^	14	^	15a	·	15b	^	16	^	17a	·	17b	^	18	^	19	^	20	^	21a	·	21b	·	21c	^
SP1:
SP2:
SP3:																									-
SP4:
SP5:			-

ExUns:	22a	·	22b	^	23	^	24	^	25	^	26	^	27	^	28	^	29	^	30	^	31	^	32	^	33	^	34	^	35	^	36a	·	36b	^	37	^	38	^	39	^	40	^	41	^	42	^	43	^	44	^	45	^
SP1:											-																										-										-
SP2:																																					-										-
SP3:
SP4:
SP5:

ExUns:	46a	·	46b	^	47	^	48	^	49a	·	49b	^	50	^	51	^	52	^	53	^	54a	·	54b	^	55	^	56	^	57	^	58	^	59	^	60a	·	60b	^	61	^	62	^	63	^	64a	·	64b	^	65	^	66a	·
SP1:									-																										-
SP2:									-																										-
SP3:
SP4:
SP5:

About this scheme

ECgene alternative splicing isoforms for WDFY4

5 Ensembl transcripts including schematic representations:

ENST00000360890 ENST00000325239 ENST00000312002 ENST00000374161 ENST00000265453

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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WDFY4 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for WDFY4

¹ / ² / ³

4 probe-sets matching WDFY4 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
76277_at²^{, 3}	U95-E	1	1.00	1.00	1.00	1.00	AI479644	0.60	0.75	0.68	1
229597_s_at²^{, 3}	U133-B	1	1.00	1.00	--	--	BF592952	0.40	0.67	0.55	1
1553863_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
229597_s_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks WDFY4 for binary patterns associated with the probe-sets selected above

About GeneDecksing

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.287379

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for WDFY4 gene from 4 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	WDFY4¹	--	WDFY family member 4	85.85(n) 84.6(a)	486770 XM_543897.2 XP_543897.2
chimpanzee (Pan troglodytes)	WDFY4¹	--	WDFY family member 4	96.14(n) 95.17(a)	450447 XM_507779.2 XP_507779.2
zebrafish (Danio rerio)	LOC572477¹	--	similar to blue cheese CG14001-PA	55.35(n) 46.7(a)	572477 XM_696196.3 XP_701288.3
African clawed frog (Xenopus laevis)	CD326631.1²	--	--	73.82(n)	CD326631.1

About this table Species with no ortholog for WDFY4

ENSEMBL Gene Tree for WDFY4

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for WDFY4 gene

NSMAF² LYST² WDFY3²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/1387 NCBI SNPs in WDFY4 are shown (see all 1387 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 681)

AB	SNP ID	Valid	Chr 10 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--			--
	rs2292584^1,2	A,C,F,H	49856421(-)	`GAGCCG/AGGGGC`	1	L/P	mis¹ ese³		11	EA EU WA NA	2492
	rs2170132^1,2	A,C,F,H	49683408(+)	`AGATCC/TCCACC`	1	S/P	mis¹ ese³		7	EU EA WA NA	556
	rs6537579^1,2	A,C,F,H	49704839(+)	`GCCTCG/AGCCTT`	1	S/G	mis¹ ese³		8	EU EA WA	838
--	rs7072606^1,2	A,C,F,H	49603980(+)	`TCCTCT/CCAGGA`	1	P/S	mis¹ ese³		8	EU EA WA	840
	rs7097397^1,2	A,C,F,H	49695402(+)	`GTGGCG/AAGCCC`	1	Q/R	mis¹ ese³		11	NA EU EA WA	922
	rs3747871^1,2	C,F,H	49710638(-)	`CAGTGA/GCTTCT`	1	S/P	mis¹ ese³		9	EA EU WA	2326
	rs4838433^1,2	A,F,H	49861346(+)	`CTCCAC/TAACCT`	1	--	ng3¹		4	EU EA WA	390
	rs12360283^1,2	C,F,H	49861095(+)	`AGGCAG/AGAAGG`	1	--	ng3¹		8	EU EA WA	838
	rs11101485^1,2	C,F,H	49667994(+)	`TGTCAG/AGGTCT`	1	R/G	mis¹ ese³		8	EU EA WA	818
	rs12242384^1,2	C,F,H	49653825(+)	`TTCATC/TTCACA`	1	S/F	mis¹ ese³		8	EU EA WA	838

About this table

HapMap Linkage Disequilibrium images for WDFY4 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10 PubMed articles for WDFY4 gene:

Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10997877)^{2, 3, 4} Nagase T....Ohara O. (2000)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{3, 4} Ota T....Sugano S. (2004)
Genetic variants near TNFAIP3 on 6q23 are associated with systemic lupus erythematosus. (PubMed id 18677312)³ Graham R.R....Gaffney P.M. (2008)
Molecular genetics of successful smoking cessation: convergent genome-wide association study results. (PubMed id 18519826)³ Uhl G.R....Lerman C. (2008)
Identification and characterization of ARHGAP24 and ARHGAP25 genes in silico. (PubMed id 15254788)⁴ Katoh M. and Katoh M. (2004)
The DNA sequence and comparative analysis of human chromosome 10. (PubMed id 15164054)⁴ Deloukas P.... Rogers J. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
The sequence of the human genome. (PubMed id 11181995)³ Venter J.C....Zhu X. (2001)
Normalization and subtraction: two approaches to facilitate gene discovery. (PubMed id 8889548)³ Bonaldo M.F....Soares M.B. (1996)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 57705	HGNC: 29323	Ensembl:ENSG00000128815	euGenes: HUgn57705	ECgene: WDFY4
H-InvDB: WDFY4

(According to HUGE)
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HUGE: KIAA1607

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for WDFY4
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for WDFY4:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for WDFY4-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Browse siRNAs at Sigma-Aldrich		Browse antibodies at Sigma-Aldrich
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	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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	Custom Service for Rabbit Pab from Full-length Protein

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Search OriGene for WDFY4

Search Tocris compounds for WDFY4

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Search Novus for antibodies for WDFY4

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WDFY4 Gene at Home site.
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WDFY4 Gene

protein-coding GIFtS: 35

Aliases &
Descriptions
for WDFY4

Summaries
for WDFY4

Genomic Location
for WDFY4

Proteins
for WDFY4

Protein Domains/
Families
for WDFY4

Gene Function
for WDFY4

Pathways & Interactions
for WDFY4

Drugs & Compounds
for WDFY4

Transcripts
for WDFY4

Expression
for WDFY4

Orthologs
for WDFY4

Paralogs
for WDFY4

SNPs/Variants
for WDFY4

Disorders & Mutations
for WDFY4

Medical News
for WDFY4

Publications
for WDFY4

Search for WDFY4

Genome Databases showing WDFY4

Other Databases showing WDFY4

Specialized Databases showing WDFY4

Licensable Technologies
for WDFY4

Services
for WDFY4

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

WDFY4 Gene

protein-coding GIFtS: 35

Aliases & Descriptionsfor WDFY4

Summariesfor WDFY4

Genomic Locationfor WDFY4

Proteinsfor WDFY4

Protein Domains/Familiesfor WDFY4

Gene Functionfor WDFY4

Pathways & Interactionsfor WDFY4

Drugs & Compoundsfor WDFY4

Transcriptsfor WDFY4

Expression for WDFY4

Orthologsfor WDFY4

Paralogsfor WDFY4

SNPs/Variantsfor WDFY4

Disorders & Mutationsfor WDFY4

Medical Newsfor WDFY4

Publicationsfor WDFY4

Search for WDFY4

Genome Databases showing WDFY4

Other Databases showing WDFY4

Specialized Databases showing WDFY4

Licensable Technologiesfor WDFY4

Servicesfor WDFY4

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for WDFY4

Summaries
for WDFY4

Genomic Location
for WDFY4

Proteins
for WDFY4

Protein Domains/
Families
for WDFY4

Gene Function
for WDFY4

Pathways & Interactions
for WDFY4

Drugs & Compounds
for WDFY4

Transcripts
for WDFY4

Expression
for WDFY4

Orthologs
for WDFY4

Paralogs
for WDFY4

SNPs/Variants
for WDFY4

Disorders & Mutations
for WDFY4

Medical News
for WDFY4

Publications
for WDFY4

Licensable Technologies
for WDFY4

Services
for WDFY4