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C10orf58 Gene

protein-coding GIFtS: 44

GC10P082158

chromosome 10 open reading frame 58
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
FLJ12536 ^²
FLJ14952 ^²
FLJ44312 ^²
MGC4248 ^¹, ²
OTTHUMP00000019973 ^²
OTTHUMP00000019975 ^²
OTTHUMP00000019976 ^²
OTTHUMP00000019978 ^²

Descriptions
chromosome 10 open reading frame 58 ^²
hypothetical protein LOC84293 ^²

External Ids
HGNC: 28651¹
Entrez Gene: 84293²
UniProtKB: Q9BRX8³
Ensembl: ENSG00000122378⁷

Search outside databases for aliases for C10orf58 gene

Previous GC identifer: GC10P081833

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Gene Wiki entry for C10orf58

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the C10orf58 gene

Entrez Gene cytogenetic band: 10q23.1 Ensembl cytogenetic band: 10q23.1 HGNC chromosome: 10

C10orf58 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10 GeneLoc Exon Structure

GeneLoc location for GC10P082158: (about GC identifiers)

Start:	82,157,565 bp from pter
End:	82,182,733 bp from pter
Size:	25,169 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000010.9 NT_030059.12

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: CJ058_HUMAN, Q9BRX8 (See protein sequence)

Recommended Name: Uncharacterized protein C10orf58 precursor

Size: 229 amino acids; 25764 Da

Subcellular location: Secreted (Potential)

Secondary accessions: Q6UW08 Q8N2K3 Q8NBK9 Q96JR0

REFSEQ proteins: NP_115709.3

ENSEMBL proteins:

ENSP00000361262 ENSP00000378012 ENSP00000361259 ENSP00000361254 ENSP00000361261

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
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	Browse Abcam for Recombinant Proteins
	Browse Abnova for Human Recombinant Proteins

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005576	extracellular region	IEA	--
GO:0005730	nucleolus	IDA	18029348
GO:0005737	cytoplasm	IDA	18029348

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Antibodies for C10orf58:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for C10orf58
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for C10orf58

Assays for C10orf58:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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ProtoNet protein and cluster: Q9BRX8

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (C10orf58)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NR_024572

Applied Biosystems Silencer® siRNAs for C10orf58

Sigma-Aldrich siRNA for C10orf58

Sigma-Aldrich shRNA for C10orf58

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for C10orf58
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_032333
Myc/DDK tagged cDNA clone in CMV expression vector: NM_032333
untagged cDNA clone in CMV expression vector: NM_032333

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_032333

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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5/8 Interacting proteins for C10orf58 (ENSP00000361261³) via UniProtKB, MINT, and/or STRING (see all 8 )

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
DYDC2	ENSP00000361272³	STRING (score=.712)
SFTPA2B	ENSP00000361390³	STRING (score=.712)
TSPAN14	ENSP00000361229³	STRING (score=.712)
ZCCHC24	ENSP00000361410³	STRING (score=.712)
SFTPA1B	ENSP00000361382³	STRING (score=.706)

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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
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Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for C10orf58

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (C10orf58)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NR_024572

Sigma-Aldrich siRNA for C10orf58

Sigma-Aldrich shRNA for C10orf58

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_032333

REFSEQ mRNAs for C10orf58 gene:

NM_032333.4

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_032333

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_032333
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_032333
                                 untagged cDNA clone in CMV expression vector: NM_032333

Additional cDNA sequence:

AK022598.1 CR619383.1

19 DOTS entries:

DT.100774232 DT.92458451 DT.419955 DT.121219134 DT.40242241 DT.203307 DT.100819121 DT.95298839

DT.100819116 DT.100819118 DT.86843636 DT.91872939 DT.95177870 DT.100819113 DT.97857471 DT.100774226

DT.121219122 DT.70101549 DT.97807124

24/300 AceView cDNA sequences (see all 300 ):

CR606562 BM558157 CR619383 BF339900 BX440079 BE742158 BP348101 AW410715

BX461369 BX381704 BU944123 BG697538 AY359059 BQ631776 BX112163 W01940

BP381588 BP381269 BP357208 CR620707 CR619721 T34417 CR618887 AA670456

highest scoring ESTs for C10orf58:

AI589570 AA155784 AA181070 AA226027 AA333590 AA357487 AA363508 AA452860 AA508485 AA632580

Unigene Clusters for C10orf58:

Chromosome 10 open reading frame 58

Hs.500333 [show with all ESTs], Hs.718589 [show with all ESTs]

Unigene Representative Sequences: NM_032333, AK022598

GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for C10orf58

ExUns:	1	^	2	^	3a	·	3b	^	4a	·	4b	·	4c	^	5	^	6	^	7a	·	7b	^	8a	·	8b	·	8c
SP1:			-		-		-						-
SP2:					-		-						-
SP3:					-		-		-				-
SP4:													-
SP5:

About this scheme

ECgene alternative splicing isoforms for C10orf58

5 Ensembl transcripts including schematic representations:

ENST00000372188 ENST00000394504 ENST00000372185 ENST00000372181 ENST00000372187

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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C10orf58 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for C10orf58

¹ / ² / ³

11 probe-sets matching C10orf58 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
62918_at²^{, 3}	U95-C	1	1.00	1.00	0.48	0.57	AI138521	0.40	1.00	0.76	1
45747_at²^{, 3}	U95-B	1	1.00	1.00	0.97	1.22	AA127810	0.20	0.20	0.20	2
42960_r_at²^{, 3}	U95-B	1	1.00	1.00	0.97	1.34	AI589570	1.00	1.00	1.00	1
57698_at²^{, 3}	U95-B	1	1.00	1.00	0.93	1.05	AL038727	0.20	1.00	0.72	1
72169_at²^{, 3}	U95-E	1	0.88	1.00	-0.03	0.85	R00953	0.80	1.00	0.91	1
224435_at²^{, 3}	U133-B	1	1.00	1.00	--	--	BC005871	0.80	1.00	0.91	1
232662_x_at²^{, 3}	U133-B	1	1.00	1.00	--	--	AK022598	0.40	1.00	0.76	1
228155_at²^{, 3}	U133-B	1	1.00	1.00	--	--	BF512388	0.60	1.00	0.82	1
224435_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
232662_x_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
228155_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks C10orf58 for binary patterns associated with the probe-sets selected above

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Data from

(Publications) and GNF BioGPS
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About these images

CGAP SAGE TAG: AGCCACAGCA

SOURCE GeneReport for Unigene clusters: Hs.500333 Hs.718589

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
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Orthologs for C10orf58 gene from 5/9 species (see all 9 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	LOC479261¹	--	similar to R53.5	87.95(n) 90.62(a)	479261 XM_536403.2 XP_536403.1
chimpanzee (Pan troglodytes)	LOC450558¹	--	similar to SFLQ611	99.27(n) 99.56(a)	450558 XM_001152855.1 XP_001152855.1
cow (Bos taurus)	C28H10orf58¹	--	chromosome 10 open reading frame 58 ortholog	88.23(n) 87.61(a)	534049 NM_001034599.1 NP_001029771.1
rat (Rattus norvegicus)	RGD1309676¹	--	similar to RIKEN cDNA 5730469M10	84.5(n) 86.84(a)	361118 NM_001014140.1 NP_001014162.1
mouse (Mus musculus)	5730469M10Rik^{1, 5}	14⁵	RIKEN cDNA 5730469M10 gene^{1, 5}	83.88(n)¹ 88.37(a)¹	70564¹ NM_027464.2¹ NP_081740.2¹ AI850090⁵ AK017688⁵ (see all 9)

About this table Species with no ortholog for C10orf58

ENSEMBL Gene Tree for C10orf58

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for C10orf58 gene

C1orf93²

Pseudogenes

2 related pseudogenes

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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10/170 NCBI SNPs in C10orf58 are shown (see all 170 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 61)

AB	SNP ID	Valid	Chr 10 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs8735^1,2	A,C,F,H,O	82182693(+)	`AAAAAA/TGTCCA`	1	--	ut3¹		21		MN NA EU EA WA	2132
--	rs7097080^1,2	A,C,F,H	82156680(+)	`TTTCCA/GCGCTG`	1	--	ng5¹		3		EU EA	300
--	rs7067984^1,2	A,C	82157101(+)	`caagcG/Tattct`	1	--	ng5¹		0	--	--	--	--
--	rs1063161^1,2	A,C	82182105(+)	`ggtgcA/Ggtggc`	2	--	ut3¹		0	--	--	--	--
--	rs7067977^1,2	A,C	82157087(+)	`ccaccG/Tccggg`	1	--	ng5¹		0	--	--	--	--
	rs1417220^1,2	C,F	82158134(-)	`TCGGGC/TGTGTG`	1	--	ng5¹		1		MN	184
--	rs7077758^1,2	A	82157179(+)	`ttttaA/Gtagag`	1	--	ng5¹		0	--	--	--	--
	rs1870142^1,2	A,C,F,H,O	82172276(+)	`AGAAAA/GGGATC`	1	--	int¹ trp³		13		EU EA WA NA	1158
	rs1870143^1,2	A,C,F,H,O	82172308(+)	`TTTCCA/GGCCAG`	1	--	int¹		12		MN EU EA WA NA	1158
	rs6585986^1,2	A,C,F,H	82161539(+)	`GCACCC/TTTTCT`	1	--	int¹		4		EU EA WA	414

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HapMap Linkage Disequilibrium images for C10orf58 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
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(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
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10/11 PubMed articles for C10orf58 gene (see all 11 ):

Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs. (PubMed id 11483580)^{2, 3, 4} Yu Y....He F. (2001)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{3, 4} Ota T....Sugano S. (2004)
The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)^{3, 4} Clark H.F....Gray A. (2003)
Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)³ Barbe L....Andersson-Svahn H. (2008)
Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)³ Kimura K.... Sugano S. (2006)
Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. (PubMed id 16303743)³ Otsuki T....Isogai T. (2005)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )⁴ Aoki H.... Hino O. (2000)
Construction and characterization of a full length-enriched and a 5'- end-enriched cDNA library. (PubMed id 9373149)³ Suzuki Y.... Sugano S. (1997)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 84293	HGNC: 28651	AceView: C10orf58	Ensembl:ENSG00000122378	euGenes: HUgn84293
ECgene: C10orf58	H-InvDB: C10orf58

(According to HUGE)
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(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Licensable Technologies for C10orf58
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for C10orf58:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for C10orf58-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for C10orf58		Antibodies for C10orf58
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for C10orf58		Browse small molecules at Sigma-Aldrich
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C10orf58 Gene

protein-coding GIFtS: 44

Aliases &
Descriptions
for C10orf58

Summaries
for C10orf58

Genomic Location
for C10orf58

Proteins
for C10orf58

Protein Domains/
Families
for C10orf58

Gene Function
for C10orf58

Pathways & Interactions
for C10orf58

Drugs & Compounds
for C10orf58

Transcripts
for C10orf58

Expression
for C10orf58

Orthologs
for C10orf58

Paralogs
for C10orf58

SNPs/Variants
for C10orf58

Disorders & Mutations
for C10orf58

Medical News
for C10orf58

Publications
for C10orf58

Search for C10orf58

Genome Databases showing C10orf58

Other Databases showing C10orf58

Specialized Databases showing C10orf58

Licensable Technologies
for C10orf58

Services
for C10orf58

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

C10orf58 Gene

protein-coding GIFtS: 44

Aliases & Descriptionsfor C10orf58

Summariesfor C10orf58

Genomic Locationfor C10orf58

Proteinsfor C10orf58

Protein Domains/Familiesfor C10orf58

Gene Functionfor C10orf58

Pathways & Interactionsfor C10orf58

Drugs & Compoundsfor C10orf58

Transcriptsfor C10orf58

Expression for C10orf58

Orthologsfor C10orf58

Paralogsfor C10orf58

SNPs/Variantsfor C10orf58

Disorders & Mutationsfor C10orf58

Medical Newsfor C10orf58

Publicationsfor C10orf58

Search for C10orf58

Genome Databases showing C10orf58

Other Databases showing C10orf58

Specialized Databases showing C10orf58

Licensable Technologiesfor C10orf58

Servicesfor C10orf58

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for C10orf58

Summaries
for C10orf58

Genomic Location
for C10orf58

Proteins
for C10orf58

Protein Domains/
Families
for C10orf58

Gene Function
for C10orf58

Pathways & Interactions
for C10orf58

Drugs & Compounds
for C10orf58

Transcripts
for C10orf58

Expression
for C10orf58

Orthologs
for C10orf58

Paralogs
for C10orf58

SNPs/Variants
for C10orf58

Disorders & Mutations
for C10orf58

Medical News
for C10orf58

Publications
for C10orf58

Licensable Technologies
for C10orf58

Services
for C10orf58