BRK1 Gene
protein-coding GIFtS: 46
GCID: GC03P010165
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|
BRICK1, SCAR/WAVE actin-nucleating complex subunit(Previous name: chromosome 3 open reading frame 10 )
(Previous symbol: C3orf10)
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Aliases
for BRK1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit1 2 | | BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit, Homolog2 | | C3orf101 2 3 | | HHBrk11 | | MDS0271 2 | | Haematopoietic Stem/Progenitor Cell Protein 3002 | | HSPC3001 | | Probable Protein BRICK12 | | Chromosome 3 Open Reading Frame 101 | | Protein BRICK12 |
Export aliases for BRK1 gene to outside databasesPrevious GC identifers: GC03P010162 GC03P010163 GC03P010164 |
Summaries
for BRK1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: BRK1_HUMAN, Q8WUW1Function: Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates theArp2/3 complex
Gene Wiki entry for BRK1 (C3orf10)
|
Genomic Views
for BRK1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000003.11 NC_018914.1 NT_022517.18
Regulatory elements:
 Search SABiosciences Regulatory transcription factor binding sites for BRK1
Other transcription factors
Search SABiosciences Chromatin IP Primers for BRK1
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat BRK1 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 3p25.3 Ensembl cytogenetic band: 3p25.3 HGNC cytogenetic band: 3p25.3BRK1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 3 GeneLoc Exon Structure GeneLoc location for GC03P010165: view genomic region
(about GC identifiers)
Start:
|
10,157,276 bp from pter |
End:
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10,168,874 bp from pter |
Size:
|
11,599 bases |
Orientation:
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plus strand |
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Proteins
for BRK1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: BRK1_HUMAN, Q8WUW1 (See
protein sequence)Recommended Name: Protein BRICK1 Size: 75 amino acids; 8745 Da
Subunit: Homotrimer when in free form. Directly interacts with WASF2. Component of the WAVE1 complex composed of ABI2,CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1
Subcellular location: Cytoplasm, cytoskeleton (By similarity)
Sequence caution: Sequence=AAF28978.1; Type=Erroneous initiation;
1 PDB 3D structure from  and Proteopedia  for BRK1:3P8C (3D)
Secondary accessions: B2R5E2 Q9P082Alternative splicing: 2 isoforms: Q8WUW1-1 Q8WUW1-2 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for BRK1: NX_Q8WUW1
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q8WUW1
BRK1 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_060932.2
ENSEMBL proteins: ENSP00000432472 ENSP00000444659 Reactome Protein details: Q8WUW1
Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table
BRK1 for ontologies About GeneDecksing
BRK1 Antibody Products:
Assay Products for BRK1: |
Protein
Domains /
Families
for BRK1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| ProtoNet protein and cluster: Q8WUW1 UniProtKB/Swiss-Prot: BRK1_HUMAN, Q8WUW1Similarity: Belongs to the BRK1 family |
Function
for BRK1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: BRK1_HUMAN, Q8WUW1Function: Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates theArp2/3 complex
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for BRK1 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for BRK1
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for BRK1 |
Animal Models:
5 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for Brk1):
BRK1 for phenotypes About GeneDecksing
|
Pathways & Interactions
for BRK1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Regulation of actin dynamics for phagocytic cup formation | | | 2 | G-protein signaling_RAC1 in cellular process | | | 3 | Immune System | | | 4 | Regulation of Actin Cytoskeleton | | | 5 | ErbB1 downstream signaling | |
Pathway sources
See GeneCards unified pathways
Show all pathways
1 EMD Millipore Pathway for BRK1
1  GeneGo (Thomson Reuters) Pathway for BRK1
3  BioSystems Pathways for BRK1
5/6 
Reactome Pathways for BRK1 (see all 6)
1 
Kegg Pathway (Kegg details for BRK1):
BRK1 for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for BRK1
5/7 Interacting proteins for BRK1 (Q8WUW12, 3) via UniProtKB, MINT, STRING, and/or I2D (see all 7)About this table
Gene Ontology (GO): 4 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0001701 | in utero embryonic development |
IEA | -- | | GO:0008284 | positive regulation of cell proliferation |
IEA | -- | | GO:0030036 | actin cytoskeleton organization |
IEA | -- | | GO:0048870 | cell motility |
IEA | -- |
BRK1 for ontologies About GeneDecksing
|
Drugs & Compounds
for BRK1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for BRK1
Search CenterWatch for drugs/clinical trials and news about BRK1
|
Transcripts
for BRK1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for BRK1 gene: NM_018462.4 Unigene Cluster for BRK1: BRICK1, SCAR/WAVE actin-nucleating complex subunit Hs.649307 [show with all ESTs]Unigene Representative Sequence: BF1027252 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000530758(uc003bvb.3) ENST00000256463
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for BRK1 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for BRK1
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript Custom all cDNA clones Services for BRK1 | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for BRK1 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat BRK1 |
Additional cDNA sequence: AF161418.1 AK290580.1 AK312155.1 AY148219.1 AY148220.1 BC001067.1 BC007929.1 BC019303.1 BC061515.1 24 DOTS entries: DT.454565 DT.100880746 DT.92047200 DT.91979578 DT.97760294 DT.91786041 DT.91908712 DT.95231276 DT.100880742 DT.120922768 DT.91763462 DT.91846616 DT.92439090 DT.100880748 DT.120922748 DT.91855549 DT.100880745 DT.120922758 DT.120922764 DT.95231265 DT.100707706 DT.101982322 DT.85104605 DT.92380635 24/582 AceView cDNA sequences (see all 582): BQ420540 NM_018462 BQ083819 BM980964 AA313963 BE710099 BM553974 AA316211 BX400053 AW105409 AI139777 BF061116 BQ631333 AI961843 BP872990 BU681150 BC019303 BQ922051 BE407654 BU660955 AW072638 BG169545 BU741409 BM795728
GeneLoc Exon Structure
|
Expression
for BRK1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| BRK1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CCCTGGCAAT
About this image
See BRK1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for BRK1
SOURCE GeneReport for Unigene cluster: Hs.649307
SABiosciences Custom PCR Arrays for BRK1
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for BRK1
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat BRK1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat BRK1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat BRK1 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for BRK1 |
Orthologs
for BRK1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for BRK1 gene from 6/17 species (see all 17) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
mouse
(Mus musculus) |
Mammalia |
Brk11 , 5 |
RIKEN cDNA 6720456B07 gene5
BRICK1, SCAR/WAVE actin-nucleating complex subunit1 |
94.67(n)1
98.67(a)1 |
|
6 (52.81 cM)5
1013141 NM_133937.11 NP_598698.11
1136047725 |
African clawed frog
(Xenopus laevis) |
Amphibia |
Xl.109062 |
Xenopus laevis transcribed sequences |
83.4(n) |
|
BJ080684.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
Dr.139252 |
Danio rerio, Similar to uncharacterized hematopoietic stem/progenitor cells protein MDS027, clone IMAGE5602716, mRNA less |
74.81(n) |
|
BC046048.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
HSPC3001 |
CG30173-PA |
69.52(n)
77.14(a) |
|
246497 NM_166648.2 NP_726400.1 |
thale cress
(Arabidopsis thaliana) |
eudicotyledons |
HSPC3006 |
protein BRICK 1 |
33(a) |
1 ↔ 1 |
2(9623068-9624218) |
rice
(Oryza sativa) |
Liliopsida |
-- |
BRICK1, putative, expressed |
36(a) |
1 ↔ 1 |
2(35673952-35675461) |
ENSEMBL Gene Tree for BRK1 (if available)
TreeFam Gene Tree for BRK1 (if available) |
Paralogs
for BRK1 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for BRK1 gene
BRK1 for paralogs About GeneDecksing
2 Pseudogenes.org Pseudogenes for BRK1
PGOHUM00000257957 PGOHUM00000260940
|
Genomic Variants
for BRK1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 3 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for BRK1 (10157276 - 10168874 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for BRK1: -- | SABiosciences Cancer Mutation PCR Assays |
|
Disorders
/ Diseases
for BRK1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
BRK1 for disorders About GeneDecksing
OMIM gene information: 611183
OMIM disorders: --
8  diseases for BRK1: About MalaCardsvon hippel-lindau disease clear cell renal cell carcinoma renal cell carcinoma squamous cell carcinoma
lung squamous cell carcinoma multiple sclerosis gout carcinoma
Export disorders for BRK1 gene to outside databases
|
Publications
for BRK1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for BRK1 gene, integrated from 9 sources (see all 23):
(articles sorted by number of sources associating them with BRK1) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Solid renal tumor severity in von Hippel Lindau disease is related to germline deletion length and location. (PubMed id 14695531)1, 3 Maranchie J.K....Linehan W.M. (2004)
- Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
- Functional dissection of lysine deacetylases reveals t hat HDAC1 and p300 regulate AMPK. (PubMed id 22318606)1 Lin Y.Y....Boeke J.D. (2012)
- A high-throughput approach for measuring temporal chan ges in the interactome. (PubMed id 22863883)1 Kristensen A.R....Foster L.J. (2012)
- Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
- A directed protein interaction network for investigat ing intracellular signal transduction. (PubMed id 21900206)1 Vinayagam A....Wanker E.E. (2011)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
- Structure and control of the actin regulatory WAVE complex. (PubMed id 21107423)2 Chen Z.... Rosen M.K. (2010)
|
External Searches for BRK1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing BRK1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing BRK1 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing BRK1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for BRK1 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for BRK1 gene:
Search GeneIP for patents involving BRK1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for BRK1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat BRK1 | | | Search QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing BRK1 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat BRK1 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat BRK1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat BRK1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat BRK1 |
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| |  |  |  |  | | | | |
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 |
 | | | BRK1 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for BRK1 |
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| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat BRK1 |
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