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Aliases for BRF2 Gene

Aliases for BRF2 Gene

  • BRF2, RNA Polymerase III Transcription Initiation Factor Subunit 2 3 5
  • BRF2, Subunit Of RNA Polymerase III Transcription Initiation Factor, BRF1-Like 2 3
  • BRF2, RNA Polymerase III Transcription Initiation Factor 50 KDa Subunit 2 3
  • B-Related Factor 2 3 4
  • HTFIIIB50 3 4
  • TFIIIB50 3 4
  • BRF-2 3 4
  • HBRFU 3 4
  • BRFU 3 4
  • RNA Polymerase III Transcription Initiation Factor BRFU 3
  • Transcription Factor IIB- Related Factor, TFIIIB50 3
  • Transcription Factor IIIB 50 KDa Subunit 3

External Ids for BRF2 Gene

Previous GeneCards Identifiers for BRF2 Gene

  • GC08P029359
  • GC08P043602
  • GC08M037557
  • GC08M037758
  • GC08M037701
  • GC08M036235

Summaries for BRF2 Gene

Entrez Gene Summary for BRF2 Gene

  • This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq, Jul 2008]

GeneCards Summary for BRF2 Gene

BRF2 (BRF2, RNA Polymerase III Transcription Initiation Factor Subunit) is a Protein Coding gene. Among its related pathways are RNA Polymerase III Transcription Initiation and PI3K / Akt Signaling.

UniProtKB/Swiss-Prot for BRF2 Gene

  • General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites (PubMed:11040218, PubMed:11121026, PubMed:11564744, PubMed:26638071). Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress (PubMed:26638071). Down-regulates expression of target genes in response to oxidative stress (PubMed:26638071). Overexpression protects cells against apoptosis in response to oxidative stress (PubMed:26638071).

Gene Wiki entry for BRF2 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for BRF2 Gene

Genomics for BRF2 Gene

Regulatory Elements for BRF2 Gene

Enhancers for BRF2 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH08G037761 1.1 ENCODE 15.3 +87.0 86991 2.1 PKNOX1 CREB3L1 ZFP64 WRNIP1 ARID4B SIN3A DMAP1 FEZF1 ZNF2 YY1 DDHD2 BRF2 ADGRA2 LOC102723701 ERLIN2 RAB11FIP1 ENSG00000255201 PLPBP
GH08G038041 1.3 ENCODE dbSUPER 11 -191.7 -191735 0.2 HNRNPUL1 ATF1 CREB3L1 AGO1 ZNF133 SIN3A DMAP1 ZNF48 ZNF2 YY1 BRF2 DDHD2 GC08P038040 ENSG00000260949
GH08G038700 1.4 Ensembl ENCODE dbSUPER 4.4 -853.1 -853120 4.7 ELF3 PKNOX1 KLF17 FEZF1 THRB RAD21 RARA YY1 ZNF366 SCRT2 TACC1 FGFR1 ENSG00000255201 LOC102723716 LETM2 ENSG00000254321 BRF2 RNF5P1
GH08G038681 0.9 Ensembl ENCODE 4.4 -833.1 -833059 2.5 NFIA CEBPB ZNF223 MNT CEBPG CHD7 NR2F2 ZKSCAN1 FOXM1 RAD51 FGFR1 ENSG00000255201 ENSG00000254321 BRF2 RNF5P1
GH08G038838 0.4 ENCODE 5 -990.0 -990000 2.0 JUND PLEKHA2 ENSG00000253645 HTRA4 TACC1 BRF2 ENSG00000253829 GC08P038829 PIR61058
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around BRF2 on UCSC Golden Path with GeneCards custom track

Promoters for BRF2 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000223588 213 1401 MLX CREB3L1 ZFP64 DMAP1 YY1 SLC30A9 ZNF143 ZNF263 SP3 NFYC

Genomic Location for BRF2 Gene

Chromosome:
8
Start:
37,843,268 bp from pter
End:
37,849,913 bp from pter
Size:
6,646 bases
Orientation:
Minus strand

Genomic View for BRF2 Gene

Genes around BRF2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
BRF2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for BRF2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for BRF2 Gene

Proteins for BRF2 Gene

  • Protein details for BRF2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9HAW0-BRF2_HUMAN
    Recommended name:
    Transcription factor IIIB 50 kDa subunit
    Protein Accession:
    Q9HAW0
    Secondary Accessions:
    • B2RD62
    • B4DFZ6
    • D3DSW6
    • Q9H2Y3
    • Q9H3B3
    • Q9NUY6

    Protein attributes for BRF2 Gene

    Size:
    419 amino acids
    Molecular mass:
    46533 Da
    Quaternary structure:
    • Component of TFIIIB complexes. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. Interacts with TBP; this interaction promotes recruitment of BRF2 to TATA box-containing promoters (PubMed:26638071). Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4.
    SequenceCaution:
    • Sequence=AAG35486.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for BRF2 Gene

    Alternative splice isoforms for BRF2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for BRF2 Gene

Post-translational modifications for BRF2 Gene

  • In response to oxidative stress, Cys-361 is reversibly oxidized to cysteine sulfenic acid. Oxidation of Cys-361 impairs formation of a ternary complex with TBP and DNA and down-regulates expression of target genes in response to oxidative stress.
  • Ubiquitination at Lys324
  • Modification sites at PhosphoSitePlus

Other Protein References for BRF2 Gene

No data available for DME Specific Peptides for BRF2 Gene

Domains & Families for BRF2 Gene

Protein Domains for BRF2 Gene

Suggested Antigen Peptide Sequences for BRF2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9HAW0

UniProtKB/Swiss-Prot:

BRF2_HUMAN :
  • Belongs to the TFIIB family.
Family:
  • Belongs to the TFIIB family.
genes like me logo Genes that share domains with BRF2: view

No data available for Gene Families for BRF2 Gene

Function for BRF2 Gene

Molecular function for BRF2 Gene

UniProtKB/Swiss-Prot Function:
General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites (PubMed:11040218, PubMed:11121026, PubMed:11564744, PubMed:26638071). Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress (PubMed:26638071). Down-regulates expression of target genes in response to oxidative stress (PubMed:26638071). Overexpression protects cells against apoptosis in response to oxidative stress (PubMed:26638071).
UniProtKB/Swiss-Prot Induction:
Down-regulated by epigallocatechin gallate (EGCG) treatment.

Gene Ontology (GO) - Molecular Function for BRF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001007 transcription factor activity, RNA polymerase III transcription factor binding IMP 26638071
GO:0001032 RNA polymerase III type 3 promoter DNA binding IDA 26638071
GO:0008270 zinc ion binding IEA --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with BRF2: view
genes like me logo Genes that share phenotypes with BRF2: view

Animal Model Products

CRISPR Products

miRNA for BRF2 Gene

miRTarBase miRNAs that target BRF2

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for BRF2 Gene

Localization for BRF2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for BRF2 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for BRF2 gene
Compartment Confidence
nucleus 5
cytosol 2

Gene Ontology (GO) - Cellular Components for BRF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005654 nucleoplasm TAS --
genes like me logo Genes that share ontologies with BRF2: view

Pathways & Interactions for BRF2 Gene

genes like me logo Genes that share pathways with BRF2: view

Gene Ontology (GO) - Biological Process for BRF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated IEA --
GO:0006352 DNA-templated transcription, initiation IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0006359 regulation of transcription from RNA polymerase III promoter IMP 26638071
GO:0034599 cellular response to oxidative stress IMP 26638071
genes like me logo Genes that share ontologies with BRF2: view

No data available for SIGNOR curated interactions for BRF2 Gene

Transcripts for BRF2 Gene

Unigene Clusters for BRF2 Gene

BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for BRF2 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b ^ 4a · 4b · 4c ^ 5 ^ 6a · 6b
SP1: - -
SP2: - - -
SP3: -
SP4:

Relevant External Links for BRF2 Gene

GeneLoc Exon Structure for
BRF2
ECgene alternative splicing isoforms for
BRF2

Expression for BRF2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for BRF2 Gene

mRNA differential expression in normal tissues according to GTEx for BRF2 Gene

This gene is overexpressed in Muscle - Skeletal (x4.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for BRF2 Gene



NURSA nuclear receptor signaling pathways regulating expression of BRF2 Gene:

BRF2

SOURCE GeneReport for Unigene cluster for BRF2 Gene:

Hs.709301

Evidence on tissue expression from TISSUES for BRF2 Gene

  • Nervous system(4.8)
  • Blood(4)
  • Eye(4)
genes like me logo Genes that share expression patterns with BRF2: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , Protein differential expression in normal tissues , Protein tissue co-expression partners , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for BRF2 Gene

Orthologs for BRF2 Gene

This gene was present in the common ancestor of chordates.

Orthologs for BRF2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia BRF2 34 35
  • 99.6 (n)
dog
(Canis familiaris)
Mammalia BRF2 34 35
  • 86.87 (n)
cow
(Bos Taurus)
Mammalia BRF2 34 35
  • 86.24 (n)
rat
(Rattus norvegicus)
Mammalia Brf2 34
  • 83.65 (n)
mouse
(Mus musculus)
Mammalia Brf2 34 16 35
  • 82.58 (n)
oppossum
(Monodelphis domestica)
Mammalia BRF2 35
  • 75 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia BRF2 35
  • 62 (a)
OneToOne
chicken
(Gallus gallus)
Aves BRF2 34
  • 63.07 (n)
-- 35
  • 57 (a)
OneToMany
-- 35
  • 56 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia BRF2 35
  • 54 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia brf2 34
  • 57.32 (n)
African clawed frog
(Xenopus laevis)
Amphibia Xl.4665 34
zebrafish
(Danio rerio)
Actinopterygii LOC100536580 34
  • 50.8 (n)
brf2 35
  • 34 (a)
OneToOne
Species where no ortholog for BRF2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for BRF2 Gene

ENSEMBL:
Gene Tree for BRF2 (if available)
TreeFam:
Gene Tree for BRF2 (if available)

Paralogs for BRF2 Gene

(1) SIMAP similar genes for BRF2 Gene using alignment to 3 proteins:

genes like me logo Genes that share paralogs with BRF2: view

No data available for Paralogs for BRF2 Gene

Variants for BRF2 Gene

Sequence variations from dbSNP and Humsavar for BRF2 Gene

SNP ID Clin Chr 08 pos Sequence Context AA Info Type
rs142395337 Uncertain significance 37,847,113(+) AACCG(C/T)GGTAT reference, missense
rs1000028986 -- 37,851,861(+) GAAGT(G/T)CAGTG upstream-variant-2KB
rs1000640594 -- 37,843,382(+) TACAA(A/G)ATGTC downstream-variant-500B, utr-variant-3-prime
rs1000785419 -- 37,847,630(+) GTTTA(A/G)GTGAT intron-variant
rs1000998758 -- 37,849,024(+) CTCAA(A/G)CAATC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for BRF2 Gene

Variant ID Type Subtype PubMed ID
nsv509261 CNV insertion 20534489
nsv528598 CNV loss 19592680
nsv951126 CNV deletion 24416366

Variation tolerance for BRF2 Gene

Residual Variation Intolerance Score: 36.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.13; 38.70% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for BRF2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
BRF2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for BRF2 Gene

Disorders for BRF2 Gene

Relevant External Links for BRF2

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
BRF2

No disorders were found for BRF2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for BRF2 Gene

Publications for BRF2 Gene

  1. BRFU, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with TATA-binding protein. (PMID: 11564744) Cabart P. … Murphy S. (J. Biol. Chem. 2001) 2 3 4 22 64
  2. The small nuclear RNA-activating protein 190 Myb DNA binding domain stimulates TATA box-binding protein-TATA box recognition. (PMID: 12621023) Hinkley C.S. … Henry R.W. (J. Biol. Chem. 2003) 3 4 22 64
  3. Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs. (PMID: 11483580) Yu Y. … He F. (Genome Res. 2001) 2 3 4 64
  4. Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2. (PMID: 26638071) Gouge J. … Vannini A. (Cell 2015) 3 4 64
  5. Differential expression of the TFIIIB subunits Brf1 and Brf2 in cancer cells. (PMID: 18700021) Cabarcas S. … Schramm L. (BMC Mol. Biol. 2008) 3 22 64

Products for BRF2 Gene

Sources for BRF2 Gene

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