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Aliases for BRF2 Gene

Aliases for BRF2 Gene

  • BRF2, RNA Polymerase III Transcription Initiation Factor 50 KDa Subunit 2 3
  • TFIIIB50 3 4 6
  • BRFU 3 4 6
  • BRF2, Subunit Of RNA Polymerase III Transcription Initiation Factor, BRF1-Like 2 3
  • B-Related Factor 2 3 4
  • HTFIIIB50 3 4
  • BRF-2 3 4
  • HBRFU 3 4
  • RNA Polymerase III Transcription Initiation Factor BRFU 3
  • Transcription Factor IIB- Related Factor, TFIIIB50 3
  • Transcription Factor IIIB 50 KDa Subunit 3

External Ids for BRF2 Gene

Previous GeneCards Identifiers for BRF2 Gene

  • GC08P029359
  • GC08P043602
  • GC08M037557
  • GC08M037758
  • GC08M037701
  • GC08M036235

Summaries for BRF2 Gene

Entrez Gene Summary for BRF2 Gene

  • This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq, Jul 2008]

GeneCards Summary for BRF2 Gene

BRF2 (BRF2, RNA Polymerase III Transcription Initiation Factor 50 KDa Subunit) is a Protein Coding gene. Diseases associated with BRF2 include lung squamous cell carcinoma. Among its related pathways are Translational Control and Gene Expression.

UniProtKB/Swiss-Prot for BRF2 Gene

  • General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.

Gene Wiki entry for BRF2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for BRF2 Gene

Genomics for BRF2 Gene

Regulatory Elements for BRF2 Gene

Genomic Location for BRF2 Gene

Start:
37,843,268 bp from pter
End:
37,849,913 bp from pter
Size:
6,646 bases
Orientation:
Minus strand

Genomic View for BRF2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for BRF2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for BRF2 Gene

Proteins for BRF2 Gene

  • Protein details for BRF2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9HAW0-BRF2_HUMAN
    Recommended name:
    Transcription factor IIIB 50 kDa subunit
    Protein Accession:
    Q9HAW0
    Secondary Accessions:
    • B2RD62
    • B4DFZ6
    • D3DSW6
    • Q9H2Y3
    • Q9H3B3
    • Q9NUY6

    Protein attributes for BRF2 Gene

    Size:
    419 amino acids
    Molecular mass:
    46533 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.;
    Quaternary structure:
    • Component of TFIIIB complex. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. BRF2 recruitment to the TATA box containing small nuclear RNA (snRNA) gene templates is TBP-dependent. Interacts with TBP; this interaction with TBP mediates its TATA-box recruitment of these promoters. Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4.
    SequenceCaution:
    • Sequence=AAG35486.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for BRF2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for BRF2 Gene

Proteomics data for BRF2 Gene at MOPED

Post-translational modifications for BRF2 Gene

  • Ubiquitination at Lys324
  • Modification sites at PhosphoSitePlus

Other Protein References for BRF2 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for BRF2 (TFIIIB50)

No data available for DME Specific Peptides for BRF2 Gene

Domains for BRF2 Gene

Protein Domains for BRF2 Gene

Suggested Antigen Peptide Sequences for BRF2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9HAW0

UniProtKB/Swiss-Prot:

BRF2_HUMAN :
  • Q9HAW0
Family:
  • Belongs to the TFIIB family.
Similarity:
  • Contains 1 TFIIB-type zinc finger.
genes like me logo Genes that share domains with BRF2: view

No data available for Gene Families for BRF2 Gene

Function for BRF2 Gene

Molecular function for BRF2 Gene

UniProtKB/Swiss-Prot Function: General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.
UniProtKB/Swiss-Prot Induction: Down-regulated by epigallocatechin gallate (EGCG) treatment.

Gene Ontology (GO) - Molecular Function for BRF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008270 zinc ion binding IEA --
genes like me logo Genes that share ontologies with BRF2: view

Animal Model Products

CRISPR Products

No data available for Enzyme Numbers (IUBMB) , Phenotypes , Animal Models , miRNA , Transcription Factor Targeting and HOMER Transcription for BRF2 Gene

Localization for BRF2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for BRF2 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for BRF2 Gene COMPARTMENTS Subcellular localization image for BRF2 gene
Compartment Confidence
nucleus 5
cytosol 2

Gene Ontology (GO) - Cellular Components for BRF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005654 nucleoplasm TAS --
genes like me logo Genes that share ontologies with BRF2: view

Pathways for BRF2 Gene

genes like me logo Genes that share pathways with BRF2: view

Gene Ontology (GO) - Biological Process for BRF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006352 DNA-templated transcription, initiation IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0006383 transcription from RNA polymerase III promoter TAS --
GO:0010467 gene expression TAS --
genes like me logo Genes that share ontologies with BRF2: view

Transcripts for BRF2 Gene

mRNA/cDNA for BRF2 Gene

Unigene Clusters for BRF2 Gene

BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for BRF2

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for BRF2 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b ^ 4a · 4b · 4c ^ 5 ^ 6a · 6b
SP1: - -
SP2: - - -
SP3: -
SP4:

Relevant External Links for BRF2 Gene

GeneLoc Exon Structure for
BRF2
ECgene alternative splicing isoforms for
BRF2

Expression for BRF2 Gene

mRNA expression in normal human tissues for BRF2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for BRF2 Gene

This gene is overexpressed in Muscle - Skeletal (4.2).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for BRF2 Gene

SOURCE GeneReport for Unigene cluster for BRF2 Gene Hs.709301

genes like me logo Genes that share expressions with BRF2: view

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt for BRF2 Gene

Orthologs for BRF2 Gene

This gene was present in the common ancestor of chordates.

Orthologs for BRF2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia BRF2 35
  • 99.6 (n)
  • 100 (a)
BRF2 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia BRF2 35
  • 86.24 (n)
  • 88.07 (a)
BRF2 36
  • 88 (a)
OneToOne
dog
(Canis familiaris)
Mammalia BRF2 35
  • 86.87 (n)
  • 89.74 (a)
BRF2 36
  • 89 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Brf2 35
  • 82.58 (n)
  • 82.58 (a)
Brf2 16
Brf2 36
  • 83 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia BRF2 36
  • 75 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia BRF2 36
  • 62 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Brf2 35
  • 83.65 (n)
  • 85.1 (a)
chicken
(Gallus gallus)
Aves -- 36
  • 56 (a)
OneToMany
-- 36
  • 57 (a)
OneToMany
BRF2 35
  • 63.07 (n)
  • 59.8 (a)
lizard
(Anolis carolinensis)
Reptilia BRF2 36
  • 54 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.4665 35
tropical clawed frog
(Silurana tropicalis)
Amphibia brf2 35
  • 57.32 (n)
  • 54.23 (a)
zebrafish
(Danio rerio)
Actinopterygii brf2 36
  • 34 (a)
OneToOne
LOC100536580 35
  • 50.8 (n)
  • 41.35 (a)
Species with no ortholog for BRF2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for BRF2 Gene

ENSEMBL:
Gene Tree for BRF2 (if available)
TreeFam:
Gene Tree for BRF2 (if available)

Paralogs for BRF2 Gene

Selected SIMAP similar genes for BRF2 Gene using alignment to 3 proteins:

genes like me logo Genes that share paralogs with BRF2: view

No data available for Paralogs for BRF2 Gene

Variants for BRF2 Gene

Sequence variations from dbSNP and Humsavar for BRF2 Gene

SNP ID Clin Chr 08 pos Sequence Context AA Info Type MAF
rs4976897 -- 37,850,380(-) AAAGC(A/G)TTTGG upstream-variant-2KB
rs6651155 -- 37,847,099(+) TGTTG(A/G)TAGTA reference, synonymous-codon
rs7008256 -- 37,846,520(+) gaggc(C/G)gaggc intron-variant
rs7816053 -- 37,849,126(+) GTTAC(A/G)TTACC intron-variant
rs11777510 -- 37,848,539(+) ACAGC(A/T)CCTGG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for BRF2 Gene

Variant ID Type Subtype PubMed ID
nsv509261 CNV Insertion 20534489
nsv528598 CNV Loss 19592680

Relevant External Links for BRF2 Gene

HapMap Linkage Disequilibrium report
BRF2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for BRF2 Gene

Disorders for BRF2 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (1) Diseases for BRF2 Gene including...

Search for BRF2 Gene in MalaCards »
genes like me logo Genes that share disorders with BRF2: view

No data available for UniProtKB/Swiss-Prot for BRF2 Gene

Publications for BRF2 Gene

  1. BRFU, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with TATA-binding protein. (PMID: 11564744) Cabart P. … Murphy S. (J. Biol. Chem. 2001) 2 3 4 23
  2. The small nuclear RNA-activating protein 190 Myb DNA binding domain stimulates TATA box-binding protein-TATA box recognition. (PMID: 12621023) Hinkley C.S. … Henry R.W. (J. Biol. Chem. 2003) 3 4 23
  3. Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs. (PMID: 11483580) Yu Y. … He F. (Genome Res. 2001) 2 3 4
  4. Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters. (PMID: 11040218) Schramm L. … Hernandez N. (Genes Dev. 2000) 3 4
  5. A stable complex of a novel transcription factor IIB- related factor, human TFIIIB50, and associated proteins mediate selective transcription by RNA polymerase III of genes with upstream promoter elements. (PMID: 11121026) Teichmann M. … Roeder R.G. (Proc. Natl. Acad. Sci. U.S.A. 2000) 3 4

Products for BRF2 Gene

Sources for BRF2 Gene

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