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Aliases for BRF2 Gene

Aliases for BRF2 Gene

  • BRF2, RNA Polymerase III Transcription Initiation Factor 50 KDa Subunit 2 3 5
  • BRF2, Subunit Of RNA Polymerase III Transcription Initiation Factor, BRF1-Like 2 3
  • B-Related Factor 2 3 4
  • HTFIIIB50 3 4
  • TFIIIB50 3 4
  • BRF-2 3 4
  • HBRFU 3 4
  • BRFU 3 4
  • RNA Polymerase III Transcription Initiation Factor BRFU 3
  • Transcription Factor IIB- Related Factor, TFIIIB50 3

External Ids for BRF2 Gene

Previous GeneCards Identifiers for BRF2 Gene

  • GC08P029359
  • GC08P043602
  • GC08M037557
  • GC08M037758
  • GC08M037701
  • GC08M036235

Summaries for BRF2 Gene

Entrez Gene Summary for BRF2 Gene

  • This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq, Jul 2008]

GeneCards Summary for BRF2 Gene

BRF2 (BRF2, RNA Polymerase III Transcription Initiation Factor 50 KDa Subunit) is a Protein Coding gene. Among its related pathways are PI3K / Akt Signaling and Translational Control.

UniProtKB/Swiss-Prot for BRF2 Gene

  • General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.

Gene Wiki entry for BRF2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for BRF2 Gene

Genomics for BRF2 Gene

Regulatory Elements for BRF2 Gene

Enhancers for BRF2 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around BRF2 on UCSC Golden Path with GeneCards custom track

Promoters for BRF2 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around BRF2 on UCSC Golden Path with GeneCards custom track

Genomic Location for BRF2 Gene

Chromosome:
8
Start:
37,843,268 bp from pter
End:
37,849,913 bp from pter
Size:
6,646 bases
Orientation:
Minus strand

Genomic View for BRF2 Gene

Genes around BRF2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
BRF2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for BRF2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for BRF2 Gene

Proteins for BRF2 Gene

  • Protein details for BRF2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9HAW0-BRF2_HUMAN
    Recommended name:
    Transcription factor IIIB 50 kDa subunit
    Protein Accession:
    Q9HAW0
    Secondary Accessions:
    • B2RD62
    • B4DFZ6
    • D3DSW6
    • Q9H2Y3
    • Q9H3B3
    • Q9NUY6

    Protein attributes for BRF2 Gene

    Size:
    419 amino acids
    Molecular mass:
    46533 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Component of TFIIIB complex. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. BRF2 recruitment to the TATA box containing small nuclear RNA (snRNA) gene templates is TBP-dependent. Interacts with TBP; this interaction with TBP mediates its TATA-box recruitment of these promoters. Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4.
    SequenceCaution:
    • Sequence=AAG35486.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for BRF2 Gene

    Alternative splice isoforms for BRF2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for BRF2 Gene

Proteomics data for BRF2 Gene at MOPED

Post-translational modifications for BRF2 Gene

  • Ubiquitination at Lys 324
  • Modification sites at PhosphoSitePlus

Other Protein References for BRF2 Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for BRF2 (TFIIIB50)

No data available for DME Specific Peptides for BRF2 Gene

Domains & Families for BRF2 Gene

Protein Domains for BRF2 Gene

Suggested Antigen Peptide Sequences for BRF2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9HAW0

UniProtKB/Swiss-Prot:

BRF2_HUMAN :
  • Belongs to the TFIIB family.
  • Contains 1 TFIIB-type zinc finger.
Family:
  • Belongs to the TFIIB family.
Similarity:
  • Contains 1 TFIIB-type zinc finger.
genes like me logo Genes that share domains with BRF2: view

No data available for Gene Families for BRF2 Gene

Function for BRF2 Gene

Molecular function for BRF2 Gene

UniProtKB/Swiss-Prot Function:
General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.
UniProtKB/Swiss-Prot Induction:
Down-regulated by epigallocatechin gallate (EGCG) treatment.
genes like me logo Genes that share phenotypes with BRF2: view

Animal Model Products

miRNA for BRF2 Gene

miRTarBase miRNAs that target BRF2

No data available for Enzyme Numbers (IUBMB) , Gene Ontology (GO) - Molecular Function , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for BRF2 Gene

Localization for BRF2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for BRF2 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for BRF2 Gene COMPARTMENTS Subcellular localization image for BRF2 gene
Compartment Confidence
nucleus 5
cytosol 2

No data available for Gene Ontology (GO) - Cellular Components for BRF2 Gene

Pathways & Interactions for BRF2 Gene

genes like me logo Genes that share pathways with BRF2: view

Gene Ontology (GO) - Biological Process for BRF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006352 DNA-templated transcription, initiation IEA --
GO:0006383 transcription from RNA polymerase III promoter TAS --
genes like me logo Genes that share ontologies with BRF2: view

No data available for SIGNOR curated interactions for BRF2 Gene

Drugs & Compounds for BRF2 Gene

No Compound Related Data Available

Transcripts for BRF2 Gene

Unigene Clusters for BRF2 Gene

BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for BRF2 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b ^ 4a · 4b · 4c ^ 5 ^ 6a · 6b
SP1: - -
SP2: - - -
SP3: -
SP4:

Relevant External Links for BRF2 Gene

GeneLoc Exon Structure for
BRF2
ECgene alternative splicing isoforms for
BRF2

Expression for BRF2 Gene

mRNA expression in normal human tissues for BRF2 Gene

mRNA differential expression in normal tissues according to GTEx for BRF2 Gene

This gene is overexpressed in Muscle - Skeletal (x4.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for BRF2 Gene



SOURCE GeneReport for Unigene cluster for BRF2 Gene Hs.709301

genes like me logo Genes that share expression patterns with BRF2: view

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , Protein differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Protein tissue co-expression partners for BRF2 Gene

Orthologs for BRF2 Gene

This gene was present in the common ancestor of chordates.

Orthologs for BRF2 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia BRF2 35
  • 86.24 (n)
  • 88.07 (a)
BRF2 36
  • 88 (a)
OneToOne
dog
(Canis familiaris)
Mammalia BRF2 35
  • 86.87 (n)
  • 89.74 (a)
BRF2 36
  • 89 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Brf2 35
  • 82.58 (n)
  • 82.58 (a)
Brf2 16
Brf2 36
  • 83 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia BRF2 35
  • 99.6 (n)
  • 100 (a)
BRF2 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Brf2 35
  • 83.65 (n)
  • 85.1 (a)
oppossum
(Monodelphis domestica)
Mammalia BRF2 36
  • 75 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia BRF2 36
  • 62 (a)
OneToOne
chicken
(Gallus gallus)
Aves BRF2 35
  • 63.07 (n)
  • 59.8 (a)
-- 36
  • 56 (a)
OneToMany
-- 36
  • 57 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia BRF2 36
  • 54 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia brf2 35
  • 57.32 (n)
  • 54.23 (a)
African clawed frog
(Xenopus laevis)
Amphibia Xl.4665 35
zebrafish
(Danio rerio)
Actinopterygii LOC100536580 35
  • 50.8 (n)
  • 41.35 (a)
brf2 36
  • 34 (a)
OneToOne
Species with no ortholog for BRF2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for BRF2 Gene

ENSEMBL:
Gene Tree for BRF2 (if available)
TreeFam:
Gene Tree for BRF2 (if available)

Paralogs for BRF2 Gene

(1) SIMAP similar genes for BRF2 Gene using alignment to 3 proteins:

genes like me logo Genes that share paralogs with BRF2: view

No data available for Paralogs for BRF2 Gene

Variants for BRF2 Gene

Sequence variations from dbSNP and Humsavar for BRF2 Gene

SNP ID Clin Chr 08 pos Sequence Context AA Info Type
rs11777510 -- 37,848,539(+) ACAGC(A/T)CCTGG intron-variant
rs12541064 -- 37,848,749(+) CAAAT(A/C)ATTGA intron-variant
rs4976897 -- 37,850,380(-) AAAGC(A/G)TTTGG upstream-variant-2KB
rs57138761 -- 37,850,719(+) CGCAG(-/GAGGCGCAG)TGAGC upstream-variant-2KB
rs6651155 -- 37,847,099(+) TGTTG(A/G)TAGTA reference, synonymous-codon

Structural Variations from Database of Genomic Variants (DGV) for BRF2 Gene

Variant ID Type Subtype PubMed ID
nsv509261 CNV Insertion 20534489
nsv528598 CNV Loss 19592680

Variation tolerance for BRF2 Gene

Residual Variation Intolerance Score: 36.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.13; 38.70% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for BRF2 Gene

HapMap Linkage Disequilibrium report
BRF2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for BRF2 Gene

Disorders for BRF2 Gene

Relevant External Links for BRF2

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
BRF2

No disorders were found for BRF2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for BRF2 Gene

Publications for BRF2 Gene

  1. BRFU, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with TATA-binding protein. (PMID: 11564744) Cabart P. … Murphy S. (J. Biol. Chem. 2001) 2 3 4 23 67
  2. Differential expression of the TFIIIB subunits Brf1 and Brf2 in cancer cells. (PMID: 18700021) Cabarcas S. … Schramm L. (BMC Mol. Biol. 2008) 3 23
  3. The small nuclear RNA-activating protein 190 Myb DNA binding domain stimulates TATA box-binding protein-TATA box recognition. (PMID: 12621023) Hinkley C.S. … Henry R.W. (J. Biol. Chem. 2003) 3 23
  4. Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs. (PMID: 11483580) Yu Y. … He F. (Genome Res. 2001) 2 3
  5. Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2. (PMID: 26638071) Gouge J. … Vannini A. (Cell 2015) 3

Products for BRF2 Gene

Sources for BRF2 Gene

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