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BRD4 Gene

protein-coding   GIFtS: 60
GCID: GC19M015348

Bromodomain Containing 4

(Previous name: bromodomain-containing 4)
  Search for BRD4
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Bromodomain Containing 41 2     HUNKI2
HUNK12 3 5     MCAP2
Bromodomain-Containing 41 2     Bromodomain-Containing Protein 42
Chromosome-Associated Protein1 2     Protein HUNK13
CAP2 5     

External Ids:    HGNC: 135751   Entrez Gene: 234762   Ensembl: ENSG000001418677   OMIM: 6087495   UniProtKB: O608853   

Export aliases for BRD4 gene to outside databases

Previous GC identifers: GC19M015727 GC19M015193 GC19M015209 GC19M014916


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for BRD4 Gene:
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes
during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two
bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been
implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory
tract carcinoma in young people. Two alternatively spliced transcript variants have been described. (provided by
RefSeq, Jul 2008)

GeneCards Summary for BRD4 Gene:
BRD4 (bromodomain containing 4) is a protein-coding gene. GO annotations related to this gene include histone acetyl-lysine binding and chromatin binding. An important paralog of this gene is BRDT.

UniProtKB/Swiss-Prot: BRD4_HUMAN, O60885
Function: Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in
transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with
acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene
transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During
interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the
P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb
complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming
it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes
phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly
acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA
polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated
histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and
subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following
phosphorylation by CK2, recruited to p53/TP53 specific target promoters
Function: Isoform B: Acts as a chromatin insulator in the DNA damage response pathway. Inhibits DNA damage
response signaling by recruiting the condensin-2 complex to acetylated histones, leading to chromatin structure
remodeling, insulating the region from DNA damage response by limiting spreading of histone H2AFX/H2A.x
phosphorylation

summary for BRD4 Gene:
Bromodomains (BRDs) are epigenetic readers that selectively recognize acetylated lysine (Kac) residues on
histone protein tails, and are the only known protein modules that target Kac residues. There are
approximately 62 unique human bromodomains, most of which can be classified according to sequence identity
into nine groups.

Gene Wiki entry for BRD4 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000019.9  NC_018930.2  NT_011295.12  
Regulatory elements:
   Regulatory transcription factor binding sites in the BRD4 gene promoter:
         RFX1   NF-1   AP-1   p53   FOXO3   FOXO3a   FOXO3b   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidBRD4 promoter sequence
   Search Chromatin IP Primers for BRD4

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat BRD4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19p13.1   Ensembl cytogenetic band:  19p13.12   HGNC cytogenetic band: 19p13.12

BRD4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
BRD4 gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M015348:  view genomic region     (about GC identifiers)

Start:
15,347,647 bp from pter      End:
15,443,356 bp from pter
Size:
95,710 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: BRD4_HUMAN, O60885 (See protein sequence)
Recommended Name: Bromodomain-containing protein 4  
Size: 1362 amino acids; 152219 Da
Subunit: Interacts with p53/TP53; the interaction is direct. Interacts (via CTD region) with CDK9 and CCNT1,
acting as an associated component of P-TEFb complex. Interacts with RELA (when acetylated at 'Lys-310').
Interacts (via NET domain) with WHSC1L1, JMJD6, CHD4, GLTSCR1 and ATAD5. Isoform B: interacts with NCAPD3 and
SMC2. Interacts with bovine papillomavirus type 1 regulatory protein E2 and human herpes virus-8 (HHV-8) protein
LANA. These interactions may serve for the tethering of viral genomes to host mitotic chromosomes allowing
successful partitioning of the viral genome during cell division. Interacts with Epstein-Barr virus (EBV) protein
EBNA1; this interaction facilitates transcriptional activation by EBNA1
Miscellaneous: Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and
Asn-433 are promising therapeutic molecules for the treatment of leukemias. JQ1, a thieno-triazolo-1,4-diazepine
derivative, and I-BET, a benzodiazepine derivative, have been tested on tumors with success (PubMed:20871596,
PubMed:21068722, PubMed:21964340). Treatment with GSK1210151A (I-BET151, a I-BET derivative) has strong effets on
mixed lineage leukemia and promotes myeloid differentiation and leukemia stem-cell depletion (PubMed:21964340)
Sequence caution: Sequence=AAC27978.1; Type=Erroneous initiation;
Selected PDB 3D structures from and Proteopedia for BRD4 (see all 68):
2I8N (3D)        2LSP (3D)        2NNU (3D)        2OSS (3D)        2OUO (3D)        2YEL (3D)    
Secondary accessions: O60433 Q4G0X8 Q86YS8 Q96PD3
Alternative splicing: 3 isoforms:  O60885-1   O60885-2   O60885-3   (Does not contain the C-terminal (CTD) region required to recruit the P-TEFb complex)

Explore the universe of human proteins at neXtProt for BRD4: NX_O60885

Explore proteomics data for BRD4 at MOPED

Post-translational modifications: 

  • Phosphorylation by CK2 disrupt the intramolecular binding between the bromo domain 2 and the NPS region and
    promotes binding between the NPS and the BID regions, leading to activate the protein and promote binding to
    acetylated histones. In absence of phosphorylation, BRD4 does not localize to p53/TP53 target gene promoters,
    phosphorylation promoting recruitment to p53/TP53 target promoters1
  • Ubiquitination2 at Lys99
  • Modification sites at PhosphoSitePlus

  • See BRD4 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_055114.1  NP_490597.1  

    ENSEMBL proteins: 
     ENSP00000263377   ENSP00000360901   ENSP00000353112   ENSP00000470481   ENSP00000470108  
     ENSP00000471240  

    BRD4 Human Recombinant Protein Products:

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    Novus Biologicals BRD4 Lysates
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    Cloud-Clone Corp. Proteins for BRD4

     
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    Search for Antibodies for BRD4 at Abcam
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    ThermoFisher Antibodies for BRD4
    LSBio Antibodies in human, mouse, rat for BRD4

    BRD4 Assay Products:

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    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for BRD4
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    Search eBioscience for ELISAs for BRD4 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    IUPHAR Guide to PHARMACOLOGY protein family classification: bromodomain containing 4
    Bromodomain kinase (BRD) family

    3 InterPro protein domains:
     IPR027353 NET_dom
     IPR018359 Bromodomain_CS
     IPR001487 Bromodomain

    Graphical View of Domain Structure for InterPro Entry O60885

    ProtoNet protein and cluster: O60885

    1 Blocks protein domain: IPB001487 Bromodomain signature

    UniProtKB/Swiss-Prot: BRD4_HUMAN, O60885
    Domain: The NET domain mediates interaction with a number of chromatin proteins involved in transcription
    regulation (WHSC1L1, JMJD6, CHD4, GLTSCR1 and ATAD5) (PubMed:21555454)
    Domain: The C-terminal (CTD) region mediates interaction and recruitment of CDK9 and CCNT1 subunits of the P-TEFb
    complex (PubMed:16109376, PubMed:16109377). It is also required for maintenance of higher-order chromatin
    structure (PubMed:22334664)
    Domain: The 2 bromo domains mediate specific binding to acetylated histones via Asn-140 and Asn-433, respectively
    (PubMed:20871596). The exact combination of modified histone tails required to recruit BRD4 to target genes is
    still unclear. The first bromo domain has high affinity for acetylated histone H4 tail, whereas the second bromo
    domain recognizes multiply acetylated marks in histone H3 (PubMed:22464331). A number of specific inhibitors bind
    competitively to acetyl-lysine-binding residues Asn-140 and Asn-433, promoting removal from acetylated histones.
    Many of these inhibitors are benzodiazepine derivatives (PubMed:22137933, PubMed:22136404, PubMed:23517011,
    PubMed:23530754)
    Similarity: Contains 2 bromo domains
    Similarity: Contains 1 NET domain


    Find genes that share domains with BRD4           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: BRD4_HUMAN, O60885
    Function: Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in
    transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with
    acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene
    transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During
    interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the
    P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb
    complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming
    it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes
    phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly
    acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA
    polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated
    histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and
    subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following
    phosphorylation by CK2, recruited to p53/TP53 specific target promoters
    Function: Isoform B: Acts as a chromatin insulator in the DNA damage response pathway. Inhibits DNA damage
    response signaling by recruiting the condensin-2 complex to acetylated histones, leading to chromatin structure
    remodeling, insulating the region from DNA damage response by limiting spreading of histone H2AFX/H2A.x
    phosphorylation

         Gene Ontology (GO): 5 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0002039p53 binding IDA--
    GO:0003677DNA binding IEA--
    GO:0003682chromatin binding IDA--
    GO:0005515protein binding IPI17023018
    GO:0070577histone acetyl-lysine binding IDA--
         
    Find genes that share ontologies with BRD4           About GenesLikeMe


    Phenotypes:
         8 GenomeRNAi human phenotypes for BRD4:
     Decreased focal adhesion (FA)   Decreased substrate adherent c  Decreased viability of wild-ty  Increased HPV18 LCR reporter a 
     Increased cell death HMECs cel  Increased cilium length after   Synthetic lethal with c-Myc af  Upregulation of Wnt/beta-caten 

         10 MGI mutant phenotypes (inferred from 1 allele(MGI details for Brd4):
     craniofacial  embryogenesis  endocrine/exocrine gland  growth/size/body  integument 
     liver/biliary system  mortality/aging  reproductive system  skeleton  vision/eye 

    Find genes that share phenotypes with BRD4           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for BRD4
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for BRD4

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for BRD4
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for BRD4

    miRNA
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    miRTarBase miRNAs that target BRD4:
    hsa-mir-320a (MIRT044471), hsa-mir-455-3p (MIRT037828), hsa-mir-877-3p (MIRT036894), hsa-mir-484 (MIRT042219), hsa-mir-2110 (MIRT035757), hsa-mir-26b-5p (MIRT029120)

    Block miRNA regulation of human, mouse, rat BRD4 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate BRD4 (see all 24):
    hsa-miR-623 hsa-miR-3910 hsa-miR-3938 hsa-miR-520d-5p hsa-miR-200a hsa-miR-1285 hsa-miR-507 hsa-miR-141
    SwitchGear 3'UTR luciferase reporter plasmidBRD4 3' UTR sequence
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for BRD4
    Predesigned siRNA for gene silencing in human, mouse, rat BRD4

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    GenScript: all cDNA clones in your preferred vector (see all 2): BRD4 (NM_058243)
    Browse Sino Biological Human cDNA Clones
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    Addgene plasmids for BRD4 

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for BRD4


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    BRD4_HUMAN, O60885: Nucleus. Chromosome. Note=Associates with acetylated chromatin. Released from chromatin upon
    deacetylation of histones that can be triggered by different signals such as activation of the JNK pathway or
    nocodazole treatment
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol3

    Gene Ontology (GO): Selected cellular component terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000790nuclear chromatin IEA--
    GO:0000794condensed nuclear chromosome IDA10938129
    GO:0005634nucleus IDA--
    GO:0005694chromosome IDA--
    GO:0005730NOT nucleolus IDA--

    Find genes that share ontologies with BRD4           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for BRD4 About    
    See pathways by source

    SuperPathContained pathways About
    1Chromatin Regulation / Acetylation
    Chromatin Regulation / Acetylation


    Find genes that share SuperPaths with BRD4           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    3 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for BRD4
        EphB-EphrinB Signaling
    Ephrin-Eph Signaling
    Insulin Receptor Pathway

    1 Cell Signaling Technology (CST) Pathway for BRD4
        Chromatin Regulation / Acetylation


        Pathway & Disease-focused RT2 Profiler PCR Array including BRD4: 
              Epigenetic Chromatin Remodeling Factors in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for BRD4

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for BRD4 (O608851, 2, 3 ENSP000002633774) via UniProtKB, MINT, STRING, and/or I2D (see all 666)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053, ENSP000003529804I2D: score=3 STRING: ENSP00000352980
    HIST1H4HP628053, ENSP000002893524I2D: score=3 STRING: ENSP00000289352
    HIST2H4AP628053, ENSP000003581624I2D: score=3 STRING: ENSP00000358162
    HIST4H4P628053, ENSP000003507674I2D: score=3 STRING: ENSP00000350767
    HIST1H4FP628053, ENSP000003669744I2D: score=3 STRING: ENSP00000366974
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 19):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000083regulation of transcription involved in G1/S transition of mitotic cell cycle IMP18039861
    GO:0001833inner cell mass cell proliferation IEA--
    GO:0006338chromatin remodeling IDA--
    GO:0006351transcription, DNA-templated IEA--
    GO:0006468protein phosphorylation IEA--

    Find genes that share ontologies with BRD4           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
    Selected ApexBio Compounds for BRD4 (see all 11)     About this table
    CompoundAction CAS #
    (+)-JQ1BET bromodomain inhibitor[1268524-70-4]
    (-)-JQ1Inactive stereoisomer of bromodomain inhibitor (+)-JQ1[1268524-71-5]
    Bromosporinebroad spectrum inhibitor for bromodomains--
    I-BET-762inhibitor for BET proteins[1260907-17-2]
    I-BET151 (GSK1210151A)BET bromodomain inhibitor(pIC50=6.1,BRD4)[1300031-49-5]
    OTX-015new potent BRD-inhibitor [202590-98-5]
    PFI-1 (PF-6405761)selective BET inhibitor for BRD4[1403764-72-6]
    RVX-208potent BET bromodomain inhibitor for BD2[1044870-39-4]
    SGC-CBP30Potent CREBBP/EP300 bromodomain inhibitor--
    UNC1215elective MBT antagonist[1415800-43-9]

    Compounds for BRD4 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    PFI 1BET bromodomain inhibitor[1403764-72-6]
    I-BET 151 hydrochlorideBET bromodomain inhibitor[1300031-49-5]
    I-CBP 112Selective CREBBP/EP300 bromodomain inhibitor--
    (+)-JQ1 Potent, selective BET bromodomain inhibitor; cell permeable [1268524-70-4]



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

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    REFSEQ mRNAs for BRD4 gene (2 alternative transcripts): 
    NM_014299.2  NM_058243.2  

    Unigene Cluster for BRD4:

    Bromodomain containing 4
    Hs.187763  [show with all ESTs]
    Unigene Representative Sequence: AF386649
    11 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000263377(uc002nar.3) ENST00000594066 ENST00000371835(uc002nas.3)
    ENST00000360016(uc002nat.3 uc002nau.4) ENST00000595926 ENST00000594841
    ENST00000602230 ENST00000594842 ENST00000597315 ENST00000601071 ENST00000601941

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    Selected qRT-PCR Assays for microRNAs that regulate BRD4 (see all 24):
    hsa-miR-623 hsa-miR-3910 hsa-miR-3938 hsa-miR-520d-5p hsa-miR-200a hsa-miR-1285 hsa-miR-507 hsa-miR-141
    SwitchGear 3'UTR luciferase reporter plasmidBRD4 3' UTR sequence
    Inhib. RNA
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    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): BRD4 (NM_058243)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for BRD4
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat BRD4
    Addgene plasmids for BRD4 
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      QuantiFast Probe-based Assays in human, mouse, rat BRD4

    Additional mRNA sequence: 

    AF252390.1 AF386649.1 AK307664.1 BC000156.1 BC008354.1 BC030158.2 BC035266.1 BC038988.1 
    BC047888.1 BC067129.1 BC091649.1 Y12059.1 

    Selected DOTS entries (see all 34):

    DT.98084068  DT.439946  DT.452411  DT.101972749  DT.121496792  DT.95334361  DT.121496740  DT.95263031 
    DT.101969817  DT.121496804  DT.40108742  DT.97773958  DT.121496826  DT.100821651  DT.121496793  DT.95364305 
    DT.91975962  DT.121496932  DT.91992517  DT.92440035  DT.92440045  DT.95262997  DT.99972180  DT.100821647 

    Selected AceView cDNA sequences (see all 502):

    BC008354 AI871347 BF904819 BU849469 AL134658 BE294807 BX095202 BQ014763 
    BM714493 AW117335 AI128514 BC030158 AI203611 BM452148 BM720402 CA777307 
    AI829021 BE378935 AI738515 AI290225 BU552971 AI383992 AA336067 T51949 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for BRD4 (see all 13)    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16a · 16b ^ 17a · 17b ^ 18 ^ 19 ^ 20 ^ 21 ^ 22 ^ 23 ^ 24a ·
    SP1:                                                              -                                                                                             
    SP2:                                                                                                                                                            
    SP3:                                                                                                                                                            
    SP4:                                                                                                                                                            
    SP5:                                                                                                              -                                             

    ExUns: 24b ^ 25 ^ 26 ^ 27 ^ 28 ^ 29 ^ 30
    SP1:                                          
    SP2:                                          
    SP3:                                          
    SP4:                                          
    SP5:                                          


    ECgene alternative splicing isoforms for BRD4

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    BRD4 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GCCCGCAAGC
    BRD4 Expression
    About this image


    BRD4 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 2) fully expand
     
     Testis (Reproductive System)
             Testis Somatic Cells Primitive Gonad
     
     Gonad (Reproductive System)
             Testis Somatic Cells Primitive Gonad
    BRD4 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    BRD4 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.187763

    UniProtKB/Swiss-Prot: BRD4_HUMAN, O60885
    Tissue specificity: Ubiquitously expressed

        Pathway & Disease-focused RT2 Profiler PCR Array including BRD4: 
              Epigenetic Chromatin Remodeling Factors in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

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    This gene was present in the common ancestor of animals.

    Orthologs for BRD4 gene from Selected species (see all 11)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Brd45 bromodomain containing 4   --   17 (17.39 cM) 32196274 
    chicken
    (Gallus gallus)
    Aves LOC1008590561 bromodomain-containing protein 4-like 77.15(n)
    81.11(a)
      100859056  XM_003643843.2  XP_003643891.2 
    lizard
    (Anolis carolinensis)
    Reptilia BRD46
    bromodomain containing 4
    81(a)
    1 ↔ 1
    2(103323245-103378309)
    zebrafish
    (Danio rerio)
    Actinopterygii brd46
    bromodomain containing 4
    55(a)
    1 ↔ 1
    3(53754194-53869362) ENSDARG00000078904
    fruit fly
    (Drosophila melanogaster)
    Insecta fs(1)h3 regulation of transcription from Pol II
    promoter more
    53(a)     --


    ENSEMBL Gene Tree for BRD4 (if available)
    TreeFam Gene Tree for BRD4 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for BRD4 gene
    BRDT2  CREBBP2  BRD22  EP3002  BRD32  
    8 SIMAP similar genes for BRD4 using alignment to 8 protein entries:     BRD4_HUMAN (see all proteins):
    BRDT    BRD3    DKFZp686N0336    BRD2    DKFZp313H139    CREBBP
    BPTF    CECR2

    Find genes that share paralogs with BRD4           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

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    Selected SNPs for BRD4 (see all 1325)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs31790151,2
    C,F--15347829(-) TTTTTA/TAATTT 1 -- ds50013Minor allele frequency- T:0.33NA 6
    rs1865909361,2
    C--15347988(+) AAAACA/CAAAAC 1 -- ds50010--------
    rs1472777841,2
    --15348217(+) TTTCAC/TACCAG 1 -- ds50010--------
    rs1169521071,2
    C--15348322(+) GCAGCC/TGCTGC 1 -- ut311Minor allele frequency- T:0.01NA 120
    rs1906007351,2
    C--15348525(+) ACCCAC/GGCAGG 1 -- ut310--------
    rs22860191,2
    C,H--15348902(+) ATGTGG/AGGATG 1 -- ut31 ese32Minor allele frequency- A:0.01NS NA 102
    rs1139917981,2
    C--15348926(+) CAGCCG/AGCACT 1 -- ut311Minor allele frequency- A:0.50CSA 2
    rs1831547471,2
    C--15348936(+) TTGCTC/TGTAAC 1 -- ut310--------
    rs1380787961,2
    C--15349056(+) GCCCCA/GGGCAT 1 -- ut310--------
    rs1870833131,2
    --15349125(+) CCGCCC/TCTAAC 1 -- ut310--------

    HapMap Linkage Disequilibrium report for BRD4 (15347647 - 15443356 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for BRD4:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv271038CNV Insertion20981092
    esv34154CNV Loss18971310
    nsv911132CNV Loss21882294

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing BRD4
    DNA2.0 Custom Variant and Variant Library Synthesis for BRD4

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

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    OMIM gene information: 608749    OMIM disorders: --

    UniProtKB/Swiss-Prot: BRD4_HUMAN, O60885
  • Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising
    in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion
    protein


  • Find genes that share disorders with BRD4           About GenesLikeMe


    Export disorders for BRD4 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for BRD4 gene, integrated from 10 sources (see all 114):
    (articles sorted by number of sources associating them with BRD4)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. A bromodomain protein, MCAP, associates with mitotic chromosomes and affects G(2)-to-M transition. (PubMed id 10938129)1, 3, 9 Dey A....Ozato K. (Mol. Cell. Biol. 2000)
    2. BRD4-NUT fusion oncogene: a novel mechanism in aggressive carcinoma. (PubMed id 12543779)1, 2, 9 French C.A....Fletcher J.A. (Cancer Res. 2003)
    3. BRD4 bromodomain gene rearrangement in aggressive carcinoma with translocation t(15;19). (PubMed id 11733348)1, 2, 9 French C.A.... Fletcher J.A. (Am. J. Pathol. 2001)
    4. Phospho switch triggers Brd4 chromatin binding and activator recruitment for gene-specific targeting. (PubMed id 23317504)1, 2 Wu S.Y.... Chiang C.M. (Mol. Cell 2013)
    5. The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. (PubMed id 23728299)1, 2 Floyd S.R.... Yaffe M.B. (Nature 2013)
    6. Bromodomain-containing protein 4 (BRD4) regulates RNA polymerase II serine 2 phosphorylation in human CD4+ T cells. (PubMed id 23086925)1, 2 Zhang W.... Chin K.C. (J. Biol. Chem. 2012)
    7. Down-regulation of NF-kappaB transcriptional activity in HIV- associated kidney disease by BRD4 inhibition. (PubMed id 22645123)1, 2 Zhang G.... Zhou M.M. (J. Biol. Chem. 2012)
    8. Bromodomain protein Brd4 associated with acetylated chromatin is important for maintenance of higher-order chromatin structure. (PubMed id 22334664)1, 2 Wang R.... You J. (J. Biol. Chem. 2012)
    9. The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. (PubMed id 21555454)1, 2 Rahman S....Howley P.M. (Mol. Cell. Biol. 2011)
    10. Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia. (PubMed id 21964340)1, 2 Dawson M.A....Kouzarides T. (Nature 2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 23476 HGNC: 13575 AceView: BRD4 Ensembl:ENSG00000141867 euGenes: HUgn23476
    ECgene: BRD4 H-InvDB: BRD4

    (According to HUGE)
    About This Section

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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for BRD4 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for BRD4 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for BRD4 gene:
    Search GeneIP for patents involving BRD4

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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