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Aliases for BRAF Gene

Aliases for BRAF Gene

  • B-Raf Proto-Oncogene, Serine/Threonine Kinase 2 3 5
  • V-Raf Murine Sarcoma Viral Oncogene Homolog B1 3 4
  • V-Raf Murine Sarcoma Viral Oncogene Homolog B 2 3
  • Proto-Oncogene B-Raf 3 4
  • BRAF1 3 4
  • RAFB1 3 4
  • B-Raf Proto-Oncogene Serine/Threonine-Protein Kinase (P94) 3
  • Murine Sarcoma Viral (V-Raf) Oncogene Homolog B1 3
  • 94 KDa B-Raf Protein 3
  • EC 2.7.11.1 4
  • B-RAF1 3
  • NS7 3
  • P94 4

External Ids for BRAF Gene

Previous GeneCards Identifiers for BRAF Gene

  • GC07M138705
  • GC07M139764
  • GC07M139841
  • GC07M139754
  • GC07M139887
  • GC07M140080
  • GC07M140424
  • GC07M134729

Summaries for BRAF Gene

Entrez Gene Summary for BRAF Gene

  • This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for BRAF Gene

BRAF (B-Raf Proto-Oncogene, Serine/Threonine Kinase) is a Protein Coding gene. Diseases associated with BRAF include cardiofaciocutaneous syndrome and lung cancer. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and Signaling by GPCR. GO annotations related to this gene include calcium ion binding and transferase activity, transferring phosphorus-containing groups. An important paralog of this gene is KSR1.

UniProtKB/Swiss-Prot for BRAF Gene

  • Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Tocris Summary for BRAF Gene

  • Raf kinases, a family of three serine/threonine kinases, are part of the Ras-MAPK signaling cascade and phosphorylate MEK. Upon growth factor stimulation, Raf-1 (c-Raf) is activated by GTP-bound Ras and recruited to the cell membrane. This activation process is tightly regulated.

Gene Wiki entry for BRAF Gene

PharmGKB "VIP" Summary for BRAF Gene

No data available for fRNAdb sequence ontologies and piRNA Summary for BRAF Gene

Genomics for BRAF Gene

Regulatory Elements for BRAF Gene

Promoters for BRAF Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around BRAF on UCSC Golden Path with GeneCards custom track

Genomic Location for BRAF Gene

Chromosome:
7
Start:
140,715,951 bp from pter
End:
140,924,764 bp from pter
Size:
208,814 bases
Orientation:
Minus strand

Genomic View for BRAF Gene

Genes around BRAF on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
BRAF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for BRAF Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for BRAF Gene

Proteins for BRAF Gene

  • Protein details for BRAF Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P15056-BRAF_HUMAN
    Recommended name:
    Serine/threonine-protein kinase B-raf
    Protein Accession:
    P15056
    Secondary Accessions:
    • A4D1T4
    • B6HY61
    • B6HY62
    • B6HY63
    • B6HY64
    • B6HY65
    • B6HY66
    • Q13878
    • Q3MIN6
    • Q9UDP8
    • Q9Y6T3

    Protein attributes for BRAF Gene

    Size:
    766 amino acids
    Molecular mass:
    84437 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1 (By similarity). Interacts with DGKH. Interacts with PRMT5. Interacts with KSR2.
    SequenceCaution:
    • Sequence=AAD43193.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAQ43111.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43112.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43113.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43114.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43115.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43116.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for BRAF Gene

neXtProt entry for BRAF Gene

Proteomics data for BRAF Gene at MOPED

Post-translational modifications for BRAF Gene

  • Methylation at Arg-671 decreases stability and kinase activity.
  • Phosphorylation at Ser-365 by SGK1 inhibits its activity.
  • Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.
  • Ubiquitination at Lys 578
  • Modification sites at PhosphoSitePlus

Other Protein References for BRAF Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for BRAF (BRAF)

No data available for DME Specific Peptides for BRAF Gene

Domains & Families for BRAF Gene

Graphical View of Domain Structure for InterPro Entry

P15056

UniProtKB/Swiss-Prot:

BRAF_HUMAN :
  • Contains 1 protein kinase domain.
  • Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
  • Contains 1 phorbol-ester/DAG-type zinc finger.
Domain:
  • Contains 1 protein kinase domain.
  • Contains 1 RBD (Ras-binding) domain.
Family:
  • Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Similarity:
  • Contains 1 phorbol-ester/DAG-type zinc finger.
genes like me logo Genes that share domains with BRAF: view

Function for BRAF Gene

Molecular function for BRAF Gene

GENATLAS Biochemistry:
murine sarcoma 3611 viral (v-raf) oncogene homolog B1 (Rmil),expressed in cerebrum and testis,fused with TIF1A to form the oncoprotein T18
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Activity is increased by EGF and HGF.
UniProtKB/Swiss-Prot Function:
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Enzyme Numbers (IUBMB) for BRAF Gene

Gene Ontology (GO) - Molecular Function for BRAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity IEA,IDA 17563371
GO:0004674 protein serine/threonine kinase activity IEA,IDA 19667065
genes like me logo Genes that share ontologies with BRAF: view

Phenotypes for BRAF Gene

MGI mutant phenotypes for BRAF:
inferred from 18 alleles
GenomeRNAi human phenotypes for BRAF:
genes like me logo Genes that share phenotypes with BRAF: view

Human Phenotype Ontology for BRAF Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for BRAF Gene

MGI Knock Outs for BRAF:

Animal Model Products

No data available for Transcription Factor Targets and HOMER Transcription for BRAF Gene

Localization for BRAF Gene

Subcellular locations from UniProtKB/Swiss-Prot for BRAF Gene

Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for BRAF Gene COMPARTMENTS Subcellular localization image for BRAF gene
Compartment Confidence
cytosol 5
nucleus 4
plasma membrane 4
mitochondrion 2

Gene Ontology (GO) - Cellular Components for BRAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular IEA --
GO:0005739 mitochondrion IEA --
genes like me logo Genes that share ontologies with BRAF: view

Pathways & Interactions for BRAF Gene

SuperPathways for BRAF Gene

Superpath Contained pathways
1 Interleukin-3, 5 and GM-CSF signaling
2 IL-7 Signaling Pathways
3 IL-2 Pathway
4 GPCR Pathway
5 Glioma
.51
.43
genes like me logo Genes that share pathways with BRAF: view

SIGNOR curated interactions for BRAF Gene

Activates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for BRAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000186 activation of MAPKK activity IEA --
GO:0002318 myeloid progenitor cell differentiation IEA --
GO:0006468 protein phosphorylation IEA,IDA 17563371
GO:0007165 signal transduction IEA --
GO:0007173 epidermal growth factor receptor signaling pathway TAS --
genes like me logo Genes that share ontologies with BRAF: view

Drugs & Compounds for BRAF Gene

(109) Drugs for BRAF Gene - From: DGIdb, Novoseek, HMDB, ClinicalTrials, ApexBio, Tocris, DrugBank, PharmGKB, and FDA Approved Drugs

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Sorafenib Approved, Investigational Pharma inhibitors, inhibitor, Target Raf kinases and tyrosine kinases inhibitor, RAF, VEGFR2, and PDGFR-beta inhibitors, Kinase Inhibitors, Vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) inhibitors 671
Dabrafenib Approved Pharma Inhibition, inhibitor, Target BRAF kinase inhibitor, Kinase Inhibitors, Mutant BRAF Kinase 70
Trametinib Approved Pharma inhibitor, Biomarker MEK 1/2 inhibitor, MEK Inhibitors, Kinase Inhibitors, Mitogen-activated protein/extracellular signal-regulated kinase (MEK) inhibitors 111
Vemurafenib Approved Pharma inhibitor, Biomarker, activator, Target BRAF kinase inhibitor, B-RAF Inhibitors, Kinase Inhibitors, BRAF inhibitors 0
Dasatinib Approved, Investigational Pharma Inhibition, inhibitor Kinase Inhibitors, SRC/BCR-ABL tyrosine kinase inhibitors 268

(34) Additional Compounds for BRAF Gene - From: HMDB, Novoseek, and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
Diglycerides Group A
  • 1,2-Dimyristoyl-rac-glycerol
  • DAG(14:0/14:0)
  • DAG(28:0)
  • DG(14:0/14:0)
  • DG(28:0)
Diglycerides Group B
  • 1-Palmitoleoyl-2-arachidonyl-sn-glycerol
  • DAG(16:1/20:0)
  • DAG(16:1n7/20:0)
  • DAG(16:1w7/20:0)
  • DAG(36:1)
Diglycerides Group C
  • 1-Myristoyl-3-stearoyl-sn-glycerol
  • 1-Tetradecanoyl-3-octadecanoyl-sn-glycerol
  • DAG(14:0/0:0/18:0)
  • DAG(32:0)
  • DG(32:0)
Diglycerides Group D
  • 1,2-Didocosahexaenoyl-rac-glycerol
  • DAG(22:6/22:6)
  • DAG(22:6n3/22:6n3)
  • DAG(22:6w3/22:6w3)
  • DAG(44:12)

(5) Tocris Compounds for BRAF Gene

Compound Action Cas Number
GDC 0879 Potent B-Raf inhibitor 905281-76-7
GW 5074 Potent, selective c-Raf1 kinase inhibitor 220904-83-6
L-779,450 Potent Raf kinase inhibitor 303727-31-3
SB 590885 Potent B-Raf inhibitor 405554-55-4
ZM 336372 Potent, selective c-Raf inhibitor 208260-29-1

(10) ApexBio Compounds for BRAF Gene

Compound Action Cas Number
CEP-32496 BRAF(V600E)inhibitor,highly potent 1188910-76-0
Dabrafenib (GSK2118436) Inhibitor of BRAF(V600) mutants 1195765-45-7
Dabrafenib Mesylate (GSK-2118436) Inhibitor of BRAF(V600) mutants 1195768-06-9
GDC-0879 B-Raf inhibitor,potent and selective 905281-76-7
LGX818 RAF inhibitor,potent and selective 1269440-17-6
PLX-4720 BRAF kinase inhibitor 918505-84-7
RAF265 Multiple intracellular kinases inhibitor 927880-90-8
SB590885 Potent B-Raf inhibitor 405554-55-4
Sorafenib Tosylate Raf kinases and tyrosine kinases inhibitor 475207-59-1
TAK-632 Pan-RAF inhibitor,potent and selective 1228591-30-7
genes like me logo Genes that share compounds with BRAF: view

Drug Products

Transcripts for BRAF Gene

Unigene Clusters for BRAF Gene

V-raf murine sarcoma viral oncogene homolog B1:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for BRAF Gene

No ASD Table

Relevant External Links for BRAF Gene

GeneLoc Exon Structure for
BRAF
ECgene alternative splicing isoforms for
BRAF

Expression for BRAF Gene

mRNA expression in normal human tissues for BRAF Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for BRAF Gene

This gene is overexpressed in Fetal Brain (22.8), Frontal cortex (19.4), and Testis (12.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for BRAF Gene



SOURCE GeneReport for Unigene cluster for BRAF Gene Hs.550061

mRNA Expression by UniProt/SwissProt for BRAF Gene

P15056-BRAF_HUMAN
Tissue specificity: Brain and testis.
genes like me logo Genes that share expression patterns with BRAF: view

Protein tissue co-expression partners for BRAF Gene

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for BRAF Gene

Orthologs for BRAF Gene

This gene was present in the common ancestor of animals.

Orthologs for BRAF Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia BRAF 35
  • 95.48 (n)
  • 98.55 (a)
BRAF 36
  • 93 (a)
OneToOne
dog
(Canis familiaris)
Mammalia BRAF 35
  • 94.73 (n)
  • 98.99 (a)
BRAF 36
  • 94 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Braf 35
  • 92.6 (n)
  • 97.07 (a)
Braf 16
Braf 36
  • 89 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia BRAF 35
  • 99.78 (n)
  • 99.87 (a)
BRAF 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Braf 35
  • 91.99 (n)
  • 96.93 (a)
oppossum
(Monodelphis domestica)
Mammalia BRAF 36
  • 95 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia BRAF 36
  • 92 (a)
OneToOne
chicken
(Gallus gallus)
Aves BRAF 35
  • 85.58 (n)
  • 94.63 (a)
BRAF 36
  • 91 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia BRAF 36
  • 84 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia braf 35
  • 76.82 (n)
  • 88.86 (a)
Str.13377 35
African clawed frog
(Xenopus laevis)
Amphibia BRAF 35
zebrafish
(Danio rerio)
Actinopterygii braf 35
  • 73.32 (n)
  • 88.07 (a)
braf 36
  • 80 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta phl 37
  • 42 (a)
phl 35
  • 52.06 (n)
  • 49.14 (a)
phl 36
  • 43 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP004699 35
  • 55.01 (n)
  • 53.32 (a)
worm
(Caenorhabditis elegans)
Secernentea lin-45 37
  • 54 (a)
lin-45 35
  • 52.97 (n)
  • 50.97 (a)
lin-45 36
  • 35 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.3462 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.10630 36
  • 43 (a)
OneToMany
Species with no ortholog for BRAF:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for BRAF Gene

ENSEMBL:
Gene Tree for BRAF (if available)
TreeFam:
Gene Tree for BRAF (if available)

Paralogs for BRAF Gene