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BCAR3 Gene

protein-coding   GIFtS: 57

GC01M093739
breast cancer anti-estrogen resistance 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
KIAA0554 2
NSP2 1, 2, 3
OTTHUMP00000011959 2
OTTHUMP00000011960 2
OTTHUMP00000011961 2
SH2D3B 1, 2, 3
Descriptions
Novel SH2-containing protein 2 3
SH2 domain-containing protein 3B 3
breast cancer anti-estrogen resistance 3 2
breast cancer antiestrogen resistance 3 2
dJ1033H22.2 (breast cancer anti-estrogen resistance 3) 2
External Ids
HGNC: 9731
Entrez Gene: 84122
UniProtKB: O758153
Ensembl: ENSG000001379367
Search outside databases for aliases for BCAR3 gene

Previous GC identifers: GC01M094433 GC01M093233 GC01M093377 GC01M093499

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for BCAR3:
Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited
by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen
resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes
involved in the development of estrogen resistance. The gene encodes a component of intracellular
signal transduction that causes estrogen-independent proliferation in human breast cancer cells.
The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine
kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48.
[provided by RefSeq]

UniProtKB/Swiss-Prot: BCAR3_HUMAN, O75815
Function: May act as an adapter protein and couple activated growth factor receptors to a signaling
pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers
anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion
to extracellular matrix proteins

Gene Wiki entry for BCAR3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the BCAR3 gene  

Entrez Gene cytogenetic band: 1p22.1   Ensembl cytogenetic band:  1p22.1   HGNC cytogenetic band: 1p22.1

BCAR3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M093739:     (about GC identifiers)

Start:
93,799,936 bp from pter
End:
94,085,294 bp from pter
Size:
285,359 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000001.9  NT_032977.8  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: BCAR3_HUMAN, O75815 (See protein sequence)
Recommended Name: Breast cancer anti-estrogen resistance protein 3  
Size: 825 amino acids; 92566 Da
Subunit: Interacts with BCAR1, NEDD9, PTK2 and PTPN1 (By similarity)
Secondary accessions: Q6UW40 Q9BR50
Alternative splicing: 2 isoforms:  O75815-1   O75815-2   

Post-translational modifications:

  • Phosphorylated on tyrosine (By similarity)1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_003558.1  

    ENSEMBL proteins: 
    ENSP00000260502 ENSP00000359264 ENSP00000359263 ENSP00000359267 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (BCAR3)
    Human Recombinant Proteins from Abnova (BCAR3)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622 intracellular IEA--
    About this table

    Antibodies for BCAR3: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for BCAR3
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (BCAR3), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (BCAR3)
    Novus Biologicals Antibodies for BCAR3

    Assays for BCAR3: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR000980 SH2
     IPR019804 Ras_G-nucl-exch_fac_CS
     IPR001895 RasGRF_CDC25


       GeneDecks  BCAR3 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O75815

    ProtoNet protein and cluster: O75815

    2 Blocks protein families:
    IPB000980 SH2 domain signature
    IPB001895 Guanine-nucleotide dissociation stimulator CDC25


    UniProtKB/Swiss-Prot: BCAR3_HUMAN, O75815
    Similarity: Contains 1 Ras-GEF domain
    Similarity: Contains 1 SH2 domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (BCAR3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (BCAR3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003567

                  Applied Biosystems Silencer® siRNAs for BCAR3

                  Sigma-Aldrich siRNA for BCAR3  
                         Sigma-Aldrich shRNA for BCAR3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for BCAR3
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003567
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003567
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_003567 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_003567

    UniProtKB/Swiss-Prot: BCAR3_HUMAN, O75815
    Function: May act as an adapter protein and couple activated growth factor receptors to a signaling
    pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers
    anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion
    to extracellular matrix proteins

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005085 guanyl-nucleotide exchange factor activity IEA--
    GO:0005515 protein binding IPI17353931
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  BCAR3  (Your Favorite Gene powered by Ingenuity) 
     Integrin Signaling

       GeneDecks  BCAR3 for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  BCAR3 


    5/18 Interacting proteins for BCAR3 (O758151 ENSP000002605023) via UniProtKB, MINT, and/or STRING (see all 18 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    IMPDH2P122681EBI-702336, EBI-353389
    PABPC1P119401EBI-702336, EBI-81531
    TARSP266391EBI-702336, EBI-1042683
    BCAR1ENSP000001623303STRING (score=.981)
    ARL4AENSP000003492503STRING (score=.899)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007165 signal transduction TAS9582273
    GO:0007264 small GTPase mediated signal transduction IEA--
    GO:0042493 response to drug TAS9582273
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for BCAR3

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (BCAR3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (BCAR3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003567

                  Sigma-Aldrich siRNA for BCAR3  
                         Sigma-Aldrich shRNA for BCAR3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_003567  

    REFSEQ mRNAs for BCAR3 gene: 

    NM_003567.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_003567  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003567
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003567
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_003567 

    Additional cDNA sequence: 

    AF124250.1 AK027785.1 AK055442.1 AK301570.1 AK310970.1 AL833121.1 AY358996.1 BC028477.1 
    BC039895.1 CR601884.1 U92715.1 

    16 DOTS entries:

    DT.75191686  DT.95248561  DT.99980444  DT.100784061  DT.92426182  DT.95116328  DT.97834718  DT.100667994 
    DT.121341346  DT.97824276  DT.121341372  DT.91670568  DT.95087753  DT.99949696  DT.91751875  DT.92426183 

    24/165 AceView cDNA sequences (see all 165 ):

    AI613118 BQ015705 BU618526 BU628766 AK055442 BI824100 BQ889001 BM463861 
    BU609135 CB306590 AA112335 CB120196 BQ028469 BM849452 F01917 CB961995 
    BG110039 AF124250 BC039895 AI627302 CA502791 AI199821 CA442336 CA306476 

    highest scoring ESTs for BCAR3:

    U92715 AF124250 BC028477 BC039895 BG772336 BI553045 BQ217872 BQ897882 CB961995 AA099941 

    Unigene Cluster for BCAR3:

    Breast cancer anti-estrogen resistance 3
    Hs.36958  [show with all ESTs]
    Unigene Representative Sequence: BC028477


    GeneLoc Exon Structure

    4 Ensembl transcripts including schematic representations:
    ENST00000260502  ENST00000370244  ENST00000370243  ENST00000370247  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    BCAR3 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for BCAR3

    1 / 2 / 3

    3 probe-sets matching BCAR3 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    36812_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 U92715 1.00 1.00 1.00 1

    204032_at2, 3 U133-A 1 1.00 1.00 -- -- NM_003567 0.60 1.00 0.82 1

    204032_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  BCAR3 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AATGAAAATA

    SOURCE GeneReport for Unigene cluster: Hs.36958

    Expression variation in blood from EXPOLDB for BCAR3

    UniProtKB/Swiss-Prot: BCAR3_HUMAN, O75815
    Tissue specificity: Ubiquitously expressed. Found in several cancer cell lines, but not in
    nonmalignant breast tissue

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for BCAR3 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    BCAR31   -- breast cancer anti-estrogen resistance 3 86.91(n)
    87.12(a)
    479941  XM_848004.1  XP_853097.1 
    chimpanzee
    (Pan troglodytes)
    BCAR31   -- breast cancer anti-estrogen resistance 3 99.31(n)
    99.03(a)
    457032  XM_513571.2  XP_513571.2 
    cow
    (Bos taurus)
    BCAR31   -- breast cancer anti-estrogen resistance 3 87.01(n)
    86.77(a)
    506231  NM_001024483.1  NP_001019654.1 
    rat
    (Rattus norvegicus)
    Bcar31   -- breast cancer anti-estrogen resistance 3 84.93(n)
    85.94(a)
    310838  XM_227657.4  XP_227657.4 
    mouse
    (Mus musculus)
    Bcar31, 5 35
    breast cancer anti-estrogen resistance 31, 5 84.57(n)1
    86.08(a)1
    298151  NM_013867.11  NP_038895.11 
     AF1795665  AI1317585  (see all 10)
    About this table        Species with no ortholog for BCAR3

    ENSEMBL Gene Tree for BCAR3
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for BCAR3 gene
    SH2D3C2  SH2D3A2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/576 NCBI SNPs in BCAR3 are shown (see all 576 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 206)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 1 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs120622781,2
    A,C,F,H93820741(+) GCGCCT/CCATTC 1 G/E mis1 ese39Minor allele frequency- C:0.05EU EA WA NA 902
    rs65413891,2
    A,C,F,H93921669(+) tttatG/Aacggc 1 -- ng317Minor allele frequency- A:0.27EU EA WA NA 562
    rs65413871,2
    A,C,F,H93920620(+) gctgtA/Tttggg 1 -- ng317Minor allele frequency- T:0.30EU EA WA NA 552
    rs111649641,2
    C,F,H93920515(+) TAAAGT/CCATAG 1 -- ng314Minor allele frequency- C:0.02EU EA WA 416
    --
    rs171101071,2
    C,F,H93814182(+) CGCAGC/GTGGTG 1 Q/H mis1 ese311Minor allele frequency- G:0.01NA EU EA WA 900
    rs171100631,2
    C,F,H93799449(+) TCCAAG/ATTTTG 1 -- ng517Minor allele frequency- A:0.29NA EU EA WA 560
    rs116831,2
    C,F93799979(-) ATATGA/GAAAAT 1 -- ut311Minor allele frequency- G:0.01MN 184
    --
    rs65413881,2
    C93920721(+) gagacA/Ggagtt 1 -- ng310--------
    rs22953301,2
    C,F,H,O93809917(+) GTGGCC/TGCTCG 1 A/A syn1 ese38Minor allele frequency- T:0.11EA NA EU WA 1264
    rs37541881,2
    H93805991(+) AACCTT/AGTGAT 1 T/T syn1 ese34Minor allele frequency- A:0.00EU EA WA 414
    About this table

    HapMap Linkage Disequilibrium images for BCAR3 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 604704

    2 Novoseek disease relationships for BCAR3 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    breast cancer 46.51 8 17427198 (2), 14583477 (2), 18722344 (2), 18351453 (1) (see all 5)
    tumors 0.00 1 19075277 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/29 PubMed articles for BCAR3 gene (see all 29 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 8412 HGNC: 973 AceView: BCAR3 Ensembl:ENSG00000137936 euGenes: HUgn8412
    ECgene: BCAR3 H-InvDB: BCAR3
    (According to HUGE)
    About This Section

    HUGE: KIAA0554
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for BCAR3 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for BCAR3:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for BCAR3 Antibodies for BCAR3
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for BCAR3 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for BCAR3

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