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BANF2 Gene

protein-coding   GIFtS: 43
GCID: GC20P017680

Barrier To Autointegration Factor 2

(Previous name: chromosome 20 open reading frame 179)
(Previous symbol: C20orf179)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Barrier To Autointegration Factor 21 2     BAFL2 3
C20orf1791 2 3     Chromosome 20 Open Reading Frame 1791
Barrier-To-Autointegration Factor 22 3     BAF22
BAF-L2 3     Barrier-To-Autointegration Factor-Like Protein2

External Ids:    HGNC: 161721   Entrez Gene: 1408362   Ensembl: ENSG000001258887   UniProtKB: Q9H5033   

Export aliases for BANF2 gene to outside databases

Previous GC identifer: GC20P017630


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for BANF2 Gene:
BANF2 (barrier to autointegration factor 2) is a protein-coding gene. An important paralog of this gene is BANF1.

UniProtKB/Swiss-Prot: BAFL_HUMAN, Q9H503
Function: May play a role in BANF1 regulation and influence tissue-specific roles of BANF1




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000020.10  NT_011387.9  NC_018931.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the BANF2 gene promoter:
         AhR   GR   AP-1   STAT3   GR-alpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidBANF2 promoter sequence
   Search Chromatin IP Primers for BANF2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat BANF2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 20p12.1   Ensembl cytogenetic band:  20p12.1   HGNC cytogenetic band: 20p12.1

BANF2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
BANF2 gene location

GeneLoc information about chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20P017680:  view genomic region     (about GC identifiers)

Start:
17,674,317 bp from pter      End:
17,716,517 bp from pter
Size:
42,201 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: BAFL_HUMAN, Q9H503 (See protein sequence)
Recommended Name: Barrier-to-autointegration factor-like protein  
Size: 90 amino acids; 10309 Da
Subunit: Homodimer. Heterodimerizes with BANF1
Caution: In contrast to BANF1, it does not seems to bind DNA
Secondary accessions: D3DW25 F5H3F6 Q7Z4M6
Alternative splicing: 2 isoforms:  Q9H503-1   Q9H503-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for BANF2: NX_Q9H503

Explore proteomics data for BANF2 at MOPED


See BANF2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins (3 alternative transcripts): 
NP_001014977.2  NP_001152967.1  NP_848572.3  

ENSEMBL proteins: 
 ENSP00000439128   ENSP00000398738   ENSP00000367036   ENSP00000246090  

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(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro protein domain:
 IPR004122 BAF_prot

Graphical View of Domain Structure for InterPro Entry Q9H503

ProtoNet protein and cluster: Q9H503

1 Blocks protein domain: IPB004122 Barrier to autointegration factor

UniProtKB/Swiss-Prot: BAFL_HUMAN, Q9H503
Similarity: Belongs to the BAF family


Find genes that share domains with BANF2           About GenesLikeMe


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Molecular Function:

     UniProtKB/Swiss-Prot Summary: BAFL_HUMAN, Q9H503
Function: May play a role in BANF1 regulation and influence tissue-specific roles of BANF1

     Gene Ontology (GO): 1 molecular function term:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0003677DNA binding IEA--
     
Find genes that share ontologies with BANF2           About GenesLikeMe


Animal Models:
   genOway: Develop your customized and physiologically relevant rodent model for BANF2

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(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from UniProtKB/Swiss-Prot
BAFL_HUMAN, Q9H503: Nucleus. Cytoplasm
Subcellular locations from COMPARTMENTS: 

CompartmentConfidence
nucleus5
cytosol2
extracellular2

Gene Ontology (GO): 2 cellular component terms:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005634nucleus IEA--
GO:0005737cytoplasm IEA--

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(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for BANF2
Interactions:

    Search GeneGlobe Interaction Network for BANF2

STRING Interaction Network Preview (showing 5 interactants - click image to see 8)

Selected Interacting proteins for BANF2 (ENSP000002460904) via UniProtKB, MINT, STRING, and/or I2D (see all 8)
InteractantInteraction Details
GeneCardExternal ID(s)
CSNK1DENSP000003244644STRING: ENSP00000324464
CSNK1EENSP000003529294STRING: ENSP00000352929
TTBK1ENSP000002597504STRING: ENSP00000259750
TTBK2ENSP000002678904STRING: ENSP00000267890
VRK1ENSP000002166394STRING: ENSP00000216639
About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Small Molecules at EMD Millipore
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Browse Tocris compounds for BANF2 (BAFL)



(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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REFSEQ mRNAs for BANF2 gene (3 alternative transcripts): 
NM_001014977.3  NM_001159495.1  NM_178477.4  

Unigene Cluster for BANF2:

Barrier to autointegration factor 2
Hs.115366  [show with all ESTs]
Unigene Representative Sequence: BQ428794
5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000545418(uc010zrs.1) ENST00000427254 ENST00000377805(uc002wqa.2)
ENST00000246090(uc002wpz.2) ENST00000467330
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Additional mRNA sequence: 

BC054871.1 BC062735.1 

2 DOTS entries:

DT.309388  DT.87046979 

19 AceView cDNA sequences:

AA397912 NM_178477 AW022487 AI150321 BC062735 BV205077 AA442269 AA862555 
BC054871 AI204602 AA394107 AI066604 BU561046 AI075928 AA629341 AA382909 
BU853192 BG459855 BQ428794 

GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for BANF2    About this scheme

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b
SP1:              -                           
SP2:              -     -                     
SP3:                                          


ECgene alternative splicing isoforms for BANF2

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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BANF2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: --
BANF2 Expression
About this image

BANF2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

BANF2 Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.115366

UniProtKB/Swiss-Prot: BAFL_HUMAN, Q9H503
Tissue specificity: Expressed strongly in testis and pancreas. Also detected in brain, colon, liver, lung, ovary,
placenta, prostate, small intestine, spleen and thymus. Not detected in heart, kidney and skeletal muscle

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In Situ
Assay Products:
 

 
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(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of animals.

Orthologs for BANF2 gene from Selected species (see all 9)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
mouse
(Mus musculus)
Mammalia Banf21 , 5 barrier to autointegration factor 21, 5 85.56(n)1
87.78(a)1
  2 (70.91 cM)5
4031711  NM_207275.11  NP_997158.11 
 1440330595 
lizard
(Anolis carolinensis)
Reptilia BANF26
barrier to autointegration factor 2
46(a)
1 ↔ 1
4(74405798-74420888)
zebrafish
(Danio rerio)
Actinopterygii si:dkey-211g8.46
si:dkey-211g8.4
35(a)
many ↔ many
19(11209153-11209704) ENSDARG00000094230
worm
(Caenorhabditis elegans)
Secernentea baf-16
Protein BAF-1 (baf-1) mRNA, complete cds
37(a)
1 ↔ 1
III(9467457-9468066) WBGene00000235


ENSEMBL Gene Tree for BANF2 (if available)
TreeFam Gene Tree for BANF2 (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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Paralogs for BANF2 gene
BANF12  
1 SIMAP similar gene for BANF2 using alignment to 2 protein entries:     BAFL_HUMAN (see all proteins):
BANF1

Find genes that share paralogs with BANF2           About GenesLikeMe



(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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Selected SNPs for BANF2 (see all 1015)    About this table                                 

Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 20 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs1843229291,2
--17672364(+) TGTGCA/GCTTAT 1 -- us2k10--------
rs1884783361,2
C--17672456(+) AAGTGC/TCTGTC 1 -- us2k10--------
rs738983931,2
C,F--17672579(+) TAATCA/GTCTTG 1 -- us2k12Minor allele frequency- G:0.10WA 120
rs61057911,2
C,F,H--17672583(+) CATCTT/GGTGAC 1 -- us2k15Minor allele frequency- G:0.01NS EA NA 540
rs1474992831,2
--17672589(+) GTGACA/GCTTCT 1 -- us2k10--------
rs1136132961,2
C--17672677(+) CAAAA-/AT/TA 
        
TATAT
1 -- us2k11CSA 2
rs1150185051,2
C,F--17672762(+) CTTCTG/ACCAGC 1 -- us2k11Minor allele frequency- A:0.02WA 118
rs1811929191,2
--17672814(+) CAGCCC/TCTGAT 1 -- us2k10--------
rs1384880181,2
--17672825(+) CTGAAA/TGTGCA 1 -- us2k10--------
rs1440209821,2
--17672891(+) CTCTTA/CTTCAG 1 -- us2k10--------

HapMap Linkage Disequilibrium report for BANF2 (17674317 - 17716517 bp)

Structural Variations
      Database of Genomic Variants (DGV) variations for BANF2: --
Site Specific Mutation Identification with PCR Assays
SeqTarget long-range PCR primers for resequencing BANF2
DNA2.0 Custom Variant and Variant Library Synthesis for BANF2

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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Find genes that share disorders with BANF2           About GenesLikeMe

Genetic Association Database (GAD): BANF2
Human Genome Epidemiology (HuGE) Navigator: BANF2 (1 document)

Export disorders for BANF2 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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PubMed articles for BANF2 gene integrated from 10 sources:
(articles sorted by number of sources associating them with BANF2)
    Utopia: connect your pdf to the dynamic
world of online information

  1. Barrier-to-autointegration factor-like (BAF-L): a proposed regulator of BAF. (PubMed id 16337940)1, 2, 9 Tifft K.E.... Wilson K.L. (Exp. Cell Res. 2006)
  2. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1, 4 Rose J.E....Uhl G.R. (Mol. Med. 2010)
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
  4. The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (Nature 2001)
  5. Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening. (PubMed id 7829101)1, 2 Robertson N.G....Morton C.C. (Genomics 1994)
  6. Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (amp 2008)
  7. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 140836 HGNC: 16172 AceView: C20orf179 Ensembl:ENSG00000125888 euGenes: HUgn140836
ECgene: BANF2 H-InvDB: BANF2

(According to HUGE)
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  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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NameDescription
PharmGKB entry for BANF2 Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
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Patent Information for BANF2 gene:
Search GeneIP for patents involving BANF2

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from genOway)
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 OriGene Custom Antibody Services for BANF2   OriGene Custom Protein Services for BANF2  

 
 
 Block miRNA regulation of human, mouse, rat BANF2 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing BANF2
 DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat BANF2 Predesigned siRNA for gene silencing in human, mouse, rat BANF2
 QuantiFast Probe-based Assays in human, mouse, rat BANF2 QuantiTect SYBR Green Assays in human, mouse, rat BANF2
 Custom PCR Arrays for BANF2 Search Chromatin IP Primers for BANF2
 Pre-validated RT2 qPCR Primer Assay in human, mouse, rat BANF2  Search GeneGlobe Interaction Network for BANF2
 Regulatory tfbs in BANF2 promoter
 GenScript Custom Purified and Recombinant Proteins Services for BANF2 GenScript cDNA clones with any tag delivered in your preferred vector for BANF2
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 Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
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 Search Tocris compounds for BANF2 (BAFL)
 Browse Sino Biological Proteins
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 Search Sino Biological for antibodies, proteins & pathways
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 Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

 Novus Tissue Slides
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 See all of Abcam's Antibodies, Kits and Proteins for BANF2
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 Browse ESI BIO Cell Lines and PureStem Progenitors for BANF2
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 Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for BANF2
 Browse SwitchGear 3'UTR luciferase reporter plasmids for BANF2
 SwitchGear Promoter luciferase reporter plasmids for BANF2
 Search ThermoFisher Antibodies for BANF2
 Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat BANF2
 Browse compounds at ApexBio
 Search Addgene for plasmids for BANF2
  Search eBioscience for proteins for BANF2
  Search eBioscience for elisas for BANF2
  eBioscience FlowRNA Probe Sets
 genOway: Develop your customized and physiologically relevant rodent model for BANF2
 antibodies-online antibodies for BANF2 (2 products)
 antibodies-online kits for BANF2 (3 products)
 antibodies-online peptides for BANF2
 antibodies-online proteins for BANF2 (2 products)
       
GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014 , 7 Oct 2014

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