V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

BAG3 Gene

protein-coding   GIFtS: 54

GC10P121400
BCL2-associated athanogene 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
BAG-3 2, 3
BIS 2, 3
CAIR-1 2
MGC104307 2
OTTHUMP00000020599 2
Descriptions
BAG-family molecular chaperone regulator-3 2
BCL2-associated athanogene 3 2
BCL2-binding athanogene 3 2
Bcl-2-associated athanogene 3 3
Bcl-2-binding protein 2
Bcl-2-binding protein Bis 3
Docking protein CAIR-1 2, 3
External Ids
HGNC: 9391
Entrez Gene: 95312
UniProtKB: O958173
Ensembl: ENSG000001519297
Search outside databases for aliases for BAG3 gene

Previous GC identifers: GC10P120329 GC10P120654 GC10P121542 GC10P121075

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for BAG3:
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate
release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus
but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW
domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of
BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian
cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its
chaperone activity in a Hip-repressible manner. [provided by RefSeq]

UniProtKB/Swiss-Prot: BAG3_HUMAN, O95817
Function: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has
anti-apoptotic activity

Gene Wiki entry for BAG3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the BAG3 gene  

Entrez Gene cytogenetic band: 10q25.2-q26.2   Ensembl cytogenetic band:  10q26.11   HGNC cytogenetic band: 10q25.2-q26.2

BAG3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10P121400:     (about GC identifiers)

Start:
121,400,872 bp from pter
End:
121,427,321 bp from pter
Size:
26,450 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000010.9  NT_030059.12  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: BAG3_HUMAN, O95817 (See protein sequence)
Recommended Name: BAG family molecular chaperone regulator 3  
Size: 575 amino acids; 61595 Da
Subunit: Binds to the ATPase domain of HSP70/HSC chaperones. Binds to Bcl-2 and PLC-gamma
Secondary accessions: A8K5L8 Q3B763 Q9NT20 Q9P120

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_004272.2  

    ENSEMBL proteins: 
    ENSP00000358081 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Bag3)
    Human Recombinant Proteins from Abnova (BAG3)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005575 cellular_component ND--
    GO:0005829 cytosol IDA10597216
    About this table

    Antibodies for BAG3: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for BAG3
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Bag3), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (BAG3)
    Novus Biologicals Antibodies for BAG3

    Assays for BAG3: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR003103 Apoptosis_reg_Bcl-2_prot_BAG
     IPR001202 WW_Rsp5_WWP


       GeneDecks  BAG3 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O95817

    ProtoNet protein and cluster: O95817

    1 Blocks protein family: IPB003103 Apoptosis regulator Bcl-2 protein

    UniProtKB/Swiss-Prot: BAG3_HUMAN, O95817
    Similarity: Contains 1 BAG domain
    Similarity: Contains 2 WW domains

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (BAG3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (BAG3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004281

                  Applied Biosystems Silencer® siRNAs for BAG3

                  Sigma-Aldrich siRNA for BAG3  
                         Sigma-Aldrich shRNA Panels and shRNA for BAG3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for BAG3
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004281
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004281
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_004281 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_004281

    UniProtKB/Swiss-Prot: BAG3_HUMAN, O95817
    Function: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has
    anti-apoptotic activity

    11 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Bag3):

    adipose tissuegrowth/sizehematopoietic systemhomeostasis/metabolism
    immune systemlethality-postnatalliver/biliary systemmuscleno phenotypic analysis
    respiratory system

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  BAG3 


    5/7 Interacting proteins for BAG3 (ENSP000003580813) via UniProtKB, MINT, and/or STRING (see all 7 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HSPA8ENSP000002273783STRING (score=.992)
    BCL2ENSP000003296233STRING (score=.99)
    HSPA4ENSP000003029613STRING (score=.978)
    BAG1ENSP000002241123STRING (score=.944)
    HSPB8ENSP000002819383STRING (score=.938)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006457 protein folding NAS9873016
    GO:0006916 anti-apoptosis NAS10597216
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for BAG3

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (BAG3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (BAG3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004281

                  Sigma-Aldrich siRNA for BAG3  
                         Sigma-Aldrich shRNA Panels and shRNA for BAG3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_004281  

    REFSEQ mRNAs for BAG3 gene: 

    NM_004281.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_004281  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004281
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004281
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_004281 

    Additional cDNA sequence: 

    AF071218.2 AF095193.2 AF127139.1 AK222800.1 AK291333.1 AK313026.1 AL137582.1 BC006418.1 
    BC014656.1 BC107786.1 

    10 DOTS entries:

    DT.218171  DT.100794105  DT.100794103  DT.95151661  DT.100794107  DT.95151971  DT.100663675  DT.91646179 
    DT.92438542  DT.99998637 

    24/297 AceView cDNA sequences (see all 297 ):

    BQ427693 BE409341 BU727464 CF243159 BU948817 CD557530 BI518668 BM835118 
    BF593187 BU948575 BI523980 AA280559 AI601139 BC006418 BM716292 AL137582 
    BM557325 CA843776 BU733542 AA034925 AA432265 AA282538 BU621949 AA865662 

    highest scoring ESTs for BAG3:

    H10530 AA034933 AA040866 AA043135 AA101635 AA101636 AA121931 AA121949 AA150669 AA150793 

    Unigene Cluster for BAG3:

    BCL2-associated athanogene 3
    Hs.523309  [show with all ESTs]
    Unigene Representative Sequence: NM_004281


    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for BAG3

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b
    SP1:              -     -                                 
    SP2:              -     -                                 
    SP3:                                                      

    About this scheme

    ECgene alternative splicing isoforms for BAG3

    1 Ensembl transcript including schematic representation:
    ENST00000369085  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    BAG3 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for BAG3

    1 / 2 / 3

    3 probe-sets matching BAG3 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    48971_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 H10530 1.00 1.00 1.00 1

    217911_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_004281 0.60 1.00 0.82 1

    217911_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CATTTCAGAG

    SOURCE GeneReport for Unigene cluster: Hs.523309
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for BAG3 gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    BAG31   -- BCL2-associated athanogene 3 86.35(n)
    85.19(a)
    486916  XM_544046.2  XP_544046.2 
    chimpanzee
    (Pan troglodytes)
    BAG31   -- BCL2-associated athanogene 3 99.42(n)
    98.96(a)
    450778  XM_508072.2  XP_508072.2 
    cow
    (Bos taurus)
    BAG31   -- BCL2-associated athanogene 3 85.7(n)
    86.34(a)
    782633  XM_001250897.1  XP_001250898.1 
    rat
    (Rattus norvegicus)
    Bag31   -- Bcl2-associated athanogene 3 86.34(n)
    86.51(a)
    293524  NM_001011936.1  NP_001011936.1 
    mouse
    (Mus musculus)
    Bag31, 5 75
    BCL2-associated athanogene 31, 5 85.87(n)1
    85.64(a)1
    298101  NM_013863.41  NP_038891.41 
     AA4072785  AB0415835  (see all 18)
    About this table        Species with no ortholog for BAG3

    ENSEMBL Gene Tree for BAG3
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for BAG3 gene
    BAG42  BAG52  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/199 NCBI SNPs in BAG3 are shown (see all 199 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 101)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 10 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs89461,2
    A,C,F,H,O121427002(+) TGTTTG/CAGAAG 1 -- ut31 ese318Minor allele frequency- C:0.40MN EU EA WA 2046
    rs38583401,2
    A,C,F,H121426276(+) TACACC/TTCCAA 1 P/L mis1 ese39Minor allele frequency- T:0.21EU EA WA NA 908
    rs22349621,2
    C,F,H121419623(+) AACAGT/CGTGGA 1 R/C mis1 ese36Minor allele frequency- C:0.11MN EU EA WA NA 670
    --
    rs111990651,2
    A,C121427319(+) ACTCAA/GAATGG 1 -- ut310--------
    --
    rs24206411,2
    A,C121400016(+) TCATGA/GGAGCA 1 -- ng510--------
    --
    rs111990641,2
    F,H121426270(+) AGAAGC/TTACAC 1 A/V mis1 ese34Minor allele frequency- T:0.00EU EA WA 372
    rs354344111,2
    C,F121419384(-) CCTCCC/TGGGAA 1 R/Q mis15Minor allele frequency- T:0.43NA EU EA WA 496
    rs1962951,2
    A,C,F,H121426352(+) AAGGTG/ACAGGG 1 V/V syn1 ese37Minor allele frequency- A:0.45MN EA NA EU WA 2158
    rs38583391,2
    A,C,F,H121426058(+) CCTCCT/GGGACA 1 P/P syn1 ese35Minor allele frequency- G:0.20NA EU EA WA 484
    rs1963411,2
    A,C,F,H,O121406601(+) TTGTAC/TAGTTA 1 -- int18Minor allele frequency- T:0.37MN NA EU EA WA 740
    About this table

    HapMap Linkage Disequilibrium images for BAG3 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 603883

    5 Novoseek disease relationships for BAG3 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    shock 33.02 1 17493862 (1)
    tumors 11.61 4 17164298 (2), 11513873 (1), 17996194 (1)
    leukemia 5.77 2 17493862 (1), 17164298 (1)
    necrosis 3.45 1 11513873 (1)
    cancer 2.88 5 17996194 (2), 19179333 (1), 17493862 (1), 11513873 (1)
    About this table

    Human Gene Mutation Database: BAG3

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/58 PubMed articles for BAG3 gene (see all 58 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 9531 HGNC: 939 AceView: BAG3 Ensembl:ENSG00000151929 euGenes: HUgn9531
    ECgene: BAG3 H-InvDB: BAG3
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for BAG3 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for BAG3:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for BAG3-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for BAG3 Antibodies for BAG3
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for BAG3 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
     Antibodies   Primer Pairs  
     Cell Culture Products   ELISAs  
     Flow Cytometry Kits   Protease Activity Assays & Reagents  
     Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
     Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
     Recombinant & Natural Proteins  

     Recombinant Proteins (BAG3)
     Antibodies (BAG3)
     Chimera RNAi (BAG3)
     Custom Service for Mouse Mab
     Custom Service for Rabbit Pab from Full-length Protein
      
     Search for Antibodies & Assays

     Recombinant Proteins
    (Bag3)
     Antibodies (Bag3)
     Search OriGene for BAG3
     Search Tocris compounds for BAG3




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for BAG3

    GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

    Random Gene From GIFtS : about GIFtS

    Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

    Developed at the Crown Human Genome Center & Weizmann Institute of Science



    The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
    BAG3 Gene at Home site.
    Version 2.41.1
    server5.xennexinc.com