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B4GALT5 Gene

protein-coding   GIFtS: 57

GC20M047682
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
B4Gal-T5 2
BETA4-GALT-IV 2
Beta4Gal-T5 3
EC 2.4.1.- 3
MGC138470 2
OTTHUMP00000031806 2
b4Gal-T5 3
beta4Gal-T5 2
beta4GalT-V 1, 2
gt-V 2
Descriptions
Beta-1,4-GalT II 3
Beta-1,4-GalTase 5 3
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5 3
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 5 2
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase,
polypeptide 5 2
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase,
polypeptide 5 2
UDP-galactose:beta-N-acetylglucosamine
beta-1,4-galactosyltransferase 5 3
beta-1,4-GalT IV 2
beta-1.4-galactosyltransferase V 2
beta4-GalT IV 2
External Ids
HGNC: 9281
Entrez Gene: 93342
UniProtKB: O432863
Ensembl: ENSG000001584707
Search outside databases for aliases for B4GALT5 gene

Previous GC identifers: GC20M047977 GC20M048887 GC20M048935 GC20M048936

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for B4GALT5:
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II
membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate
UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc,
Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different
glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal
hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains
uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four
groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and
beta4GalT7. The function of the enzyme encoded by this gene is not clear. This gene was previously
designated as B4GALT4 but was renamed to B4GALT5. In the literature it is also referred to as
beta4GalT2. [provided by RefSeq]

UniProtKB/Swiss-Prot: B4GT5_HUMAN, O43286
Function: Responsible for the synthesis of complex-type N-linked oligosaccharides in many
glycoproteins as well as the carbohydrate moieties of glycolipids

Gene Wiki entry for B4GALT5

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the B4GALT5 gene  

Entrez Gene cytogenetic band: 20q13.1-q13.2   Ensembl cytogenetic band:  20q13.13   HGNC cytogenetic band: 20q13.1-q13.2

B4GALT5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20M047682:     (about GC identifiers)

Start:
47,682,889 bp from pter
End:
47,763,745 bp from pter
Size:
80,857 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000020.9  NT_011362.9  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: B4GT5_HUMAN, O43286 (See protein sequence)
Recommended Name: Beta-1,4-galactosyltransferase 5  
Size: 388 amino acids; 45119 Da
Cofactor: Manganese (By similarity)
Subcellular location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein.
Note=Trans cisternae of Golgi stack
Secondary accessions: Q2M394 Q9UJQ8

REFSEQ proteins: NP_004767.1  

ENSEMBL proteins: 
ENSP00000360776 


Human Recombinant Proteins 
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Recombinant Proteins from Abcam (B4GALT5)
Human Recombinant Proteins from Abnova (B4GALT5)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005794 Golgi apparatus IEA--
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
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Antibodies for B4GALT5: 
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Sigma-Aldrich Antibodies for B4GALT5
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Antibodies from Abcam (B4GALT5), each with their AbpromiseSM
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Novus Biologicals Antibodies for B4GALT5

Assays for B4GALT5: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR003859 Galactosyl_T_2


   GeneDecks  B4GALT5 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry O43286

ProtoNet protein and cluster: O43286

1 Blocks protein family: IPB003859 Metazoa galactosyltransferase

UniProtKB/Swiss-Prot: B4GT5_HUMAN, O43286
Similarity: Belongs to the glycosyltransferase 7 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (B4GALT5)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (B4GALT5)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004776

              Applied Biosystems Silencer® siRNAs for B4GALT5

              Sigma-Aldrich siRNA for B4GALT5  
                     Sigma-Aldrich shRNA for B4GALT5  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_004776
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_004776
                                 untagged cDNA clone in CMV expression vector: NM_004776 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_004776

UniProtKB/Swiss-Prot: B4GT5_HUMAN, O43286
Function: Responsible for the synthesis of complex-type N-linked oligosaccharides in many
glycoproteins as well as the carbohydrate moieties of glycolipids
Enzyme Number (IUBMB): EC 2.4.1.- 

Genatlas biochemistry entry for B4GALT5:
UDP galactose

5 MGI mutant phenotypes (inferred from 1 allele(MGI details for B4galt5):

behavior/neurologicalembryogenesisgrowth/sizelethality-postnatallethality-prenatal/perinatal

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0008378 galactosyltransferase activity TAS9597550
GO:0016757 transferase activity, transferring glycosyl groups IEA--
GO:0030145 manganese ion binding IEA--
GO:0046872 metal ion binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


2 Sigma-Aldrich "Your Favorite Gene" Pathways for  B4GALT5  (Your Favorite Gene powered by Ingenuity) 
 Keratan Sulfate Biosynthesis
 N-Glycan Biosynthesis

   GeneDecks  B4GALT5 for the pathways selected above  
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1 Kegg Pathway  (Kegg details for B4GALT5):
 hsa00512 O-Glycan biosynthesis

   GeneDecks  B4GALT5 for the pathways selected above  
About GeneDecksing
UniProtKB/Swiss-Prot: B4GT5_HUMAN, O43286
Pathway: Protein modification; protein glycosylation


5/7 Interacting proteins for B4GALT5 (ENSP000003607763) via UniProtKB, MINT, and/or STRING (see all 7 )

InteractantInteraction Details
GeneCardExternal ID(s)
GCNT1ENSP000003659203STRING (score=.899)
B4GALT1ENSP000003690553STRING (score=.819)
B4GALT4ENSP000003521443STRING (score=.807)
TP53ENSP000002693053STRING (score=.8)
SMUG1ENSP000003386063STRING (score=.75)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005975 carbohydrate metabolic process IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for B4GALT5

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (B4GALT5)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (B4GALT5)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004776

              Sigma-Aldrich siRNA for B4GALT5  
                     Sigma-Aldrich shRNA for B4GALT5  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_004776  

REFSEQ mRNAs for B4GALT5 gene: 

NM_004776.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_004776  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_004776
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_004776
                                 untagged cDNA clone in CMV expression vector: NM_004776 

Additional cDNA sequence: 

AB004550.1 AF038663.1 AF289556.1 AK054803.1 AK056333.1 BC074821.2 BC074873.2 BC104987.1 
BC112265.1 

5 DOTS entries:

DT.451617  DT.87017400  DT.95134284  DT.87017870  DT.91719572 

24/335 AceView cDNA sequences (see all 335 ):

BM969496 AA243839 AI818828 BX509324 AI093151 AA292088 CA415351 BM843323 
AW043860 BX499835 AA223526 CD366019 AI924160 AA975469 AA716159 CD674125 
AI085327 AI078366 CD674479 BM989877 AI239686 BE858968 BG057973 AI245395 

highest scoring ESTs for B4GALT5:

AB004550 AA243575 AA648382 AA736578 AA836169 AA918180 AF038663 AI017532 AI088794 AI095142 

Unigene Cluster for B4GALT5:

UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5
Hs.370487  [show with all ESTs]
Unigene Representative Sequence: NM_004776


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000371711  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

B4GALT5 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for B4GALT5

1 / 2 / 3

6 probe-sets matching B4GALT5 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

39888_at2, 3 U95-A 1 1.00 1.00 0.95 0.49 AB004550 1.00 1.00 1.00 1

56373_at2, 3 U95-B 1 1.00 1.00 0.94 1.53 AA133969 0.40 1.00 0.76 1

221484_at2, 3 U133-A 1 1.00 1.00 -- -- BF691447 0.20 1.00 0.72 1

221485_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

221484_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

221485_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TGTTTGTACA

SOURCE GeneReport for Unigene cluster: Hs.370487

Expression variation in blood from EXPOLDB for B4GALT5

UniProtKB/Swiss-Prot: B4GT5_HUMAN, O43286
Tissue specificity: Ubiquitously expressed

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for B4GALT5 gene from 5/10 species (see all 10 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
B4GALT51   -- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more 92.1(n)
96.86(a)
485920  XM_543044.2  XP_543044.2 
chimpanzee
(Pan troglodytes)
B4GALT51   -- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more 99.48(n)
99.74(a)
458324  XM_001167173.1  XP_001167173.1 
cow
(Bos taurus)
B4GALT51   -- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more 89.81(n)
96(a)
282407  XM_586129.3  XP_586129.3 
rat
(Rattus norvegicus)
B4galt51   -- UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, more 80.23(n)
82.15(a)
362275  XM_342584.3  XP_342585.3 
mouse
(Mus musculus)
B4galt51, 5 25
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, more1, 5 87.46(n)1
95.1(a)1
563361  NM_019835.21  NP_062809.21 
 AA0087725  AB0047865  (see all 25)
About this table        Species with no ortholog for B4GALT5

ENSEMBL Gene Tree for B4GALT5
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for B4GALT5 gene
B4GALT72  B4GALT62  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/629 NCBI SNPs in B4GALT5 are shown (see all 629 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 158)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 20 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs2350321,2
A,C,F,H47684154(+) ACAAAA/CAAAAC 1 -- ut315Minor allele frequency- C:0.47MN EU EA WA 600
rs9514971,2
A,C,F,H47764927(-) AGAGGC/TCATAC 1 -- ng3117Minor allele frequency- A:0.05NS NA EU EA WA 1121
rs22730861,2
C,F,H47706581(+) AGCACC/TGATTG 1 G/S mis1 ese312Minor allele frequency- A:0.00EU EA WA NA 1056
rs2350351,2
C,F,H47686321(+) GGCGTC/TGTATG 1 D/N mis1 ese312Minor allele frequency- T:0.41NS NA MN EU EA WA 1100
rs61256951,2
F,H47684546(+) cacccG/Acccat 1 -- ut31 ese33Minor allele frequency- A:0.30EA WA 266
rs169947541,2
F,H47683271(+) CAAACG/AGGGAC 1 -- ut31 ese34Minor allele frequency- A:0.05NA EU 240
rs2350341,2
A,C47685745(-) TTTTTA/TAAAAA 1 -- ut310--------
--
rs2350331,2
A,C47684638(+) accccC/Tgtctc 1 -- ut310--------
rs351952171,2
C,F47686312(-) CCTTGT/GATAAA 1 D/Y mis15Minor allele frequency- G:0.01NA EU EA WA 482
--
rs72640861,2
C,H47706542(+) CTTGGC/AATAAG 1 S/A mis14Minor allele frequency- A:0.00EU EA WA 398
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HapMap Linkage Disequilibrium images for B4GALT5 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 604016

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/16 PubMed articles for B4GALT5 gene (see all 16 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 9334 HGNC: 928 AceView: B4GALT5 Ensembl:ENSG00000158470 euGenes: HUgn9334
ECgene: B4GALT5 H-InvDB: B4GALT5
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
GGDBhttp://riodb.ibase.aist.go.jp/rcmg/ggdb/
Functional Glycomics Gateway - GTasehttp://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_440
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for B4GALT5:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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