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B4GALT4 Gene

protein-coding   GIFtS: 59

GC03M120413
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
B4Gal-T4 2
Beta4Gal-T4 3
EC 2.4.1.- 3
b4Gal-T4 3
beta4Gal-T4 1, 2
Descriptions
Beta-1,4-GalTase 4 3
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4 3
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4 2
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase,
polypeptide 4 2
UDP-galactose:beta-N-acetylglucosamine
beta-1,4-galactosyltransferase 4 3
beta-N-acetylglucosaminyl-glycolipid
beta-1,4-galactosyltransferase 4 2
External Ids
HGNC: 9271
Entrez Gene: 87022
UniProtKB: O605133
Ensembl: ENSG000001215787
Search outside databases for aliases for B4GALT4 gene

Previous GC identifers: GC03M115619 GC03M118559 GC03M120211 GC03M120251

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for B4GALT4:
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II
membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate
UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc,
Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different
glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal
hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains
uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four
groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and
beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid
biosynthesis. Two alternatively spliced transcript variants have been found for this gene.
[provided by RefSeq]

UniProtKB/Swiss-Prot: B4GT4_HUMAN, O60513
Function: Responsible for the synthesis of complex-type N-linked oligosaccharides in many
glycoproteins as well as the carbohydrate moieties of glycolipids

Gene Wiki entry for B4GALT4

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the B4GALT4 gene  

Entrez Gene cytogenetic band: 3q13.3   Ensembl cytogenetic band:  3q13.32   HGNC cytogenetic band: 3q13.3

B4GALT4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03M120413:     (about GC identifiers)

Start:
120,413,279 bp from pter
End:
120,442,442 bp from pter
Size:
29,164 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000003.10  NT_005612.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: B4GT4_HUMAN, O60513 (See protein sequence)
Recommended Name: Beta-1,4-galactosyltransferase 4  
Size: 344 amino acids; 40041 Da
Cofactor: Manganese (By similarity)
Subcellular location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein.
Note=Trans cisternae of Golgi stack
Secondary accessions: Q68D68 Q9BSW3 Q9C078

REFSEQ proteins (2 alternative transcripts): 
NP_003769.1  NP_997708.1  


ENSEMBL proteins: 
ENSP00000377360 ENSP00000352144 


Human Recombinant Proteins 
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Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (B4GALT4)
Human Recombinant Proteins from Abnova (B4GALT4)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005794 Golgi apparatus IEA--
GO:0016020 membrane IEA--
GO:0016021 integral to membrane TAS9792633
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Antibodies for B4GALT4: 
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Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for B4GALT4
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Antibodies from Abcam (B4GALT4), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (B4GALT4)
Novus Biologicals Antibodies for B4GALT4

Assays for B4GALT4: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR003859 Galactosyl_T_2


   GeneDecks  B4GALT4 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry O60513

ProtoNet protein and cluster: O60513

1 Blocks protein family: IPB003859 Metazoa galactosyltransferase

UniProtKB/Swiss-Prot: B4GT4_HUMAN, O60513
Similarity: Belongs to the glycosyltransferase 7 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (B4GALT4)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (B4GALT4)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_212543

              Applied Biosystems Silencer® siRNAs for B4GALT4

              Sigma-Aldrich siRNA for B4GALT4  
                     Sigma-Aldrich shRNA for B4GALT4  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for B4GALT4
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_003778
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_003778
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_003778 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_212543

UniProtKB/Swiss-Prot: B4GT4_HUMAN, O60513
Function: Responsible for the synthesis of complex-type N-linked oligosaccharides in many
glycoproteins as well as the carbohydrate moieties of glycolipids
Catalytic activity: UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine
Biophysicochemical properties: Kinetic parameters: KM=238 uM for GlcNAc-B-S-pNP;
Enzyme Number (IUBMB): EC 2.4.1.- 

Genatlas biochemistry entry for B4GALT4:
UDP galactose

5 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003945 N-acetyllactosamine synthase activity IEA--
GO:0008378 galactosyltransferase activity TAS9792633
GO:0016757 transferase activity, transferring glycosyl groups IEA--
GO:0030145 manganese ion binding IEA--
GO:0046872 metal ion binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  B4GALT4  (Your Favorite Gene powered by Ingenuity) 
 Keratan Sulfate Biosynthesis
 N-Glycan Biosynthesis

   GeneDecks  B4GALT4 for the pathways selected above  
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2 Kegg Pathways  (Kegg details for B4GALT4):
 hsa00533 Keratan sulfate biosynthesis
 hsa00601 Glycosphingolipid biosynthesis - lacto and neolacto series

   GeneDecks  B4GALT4 for the pathways selected above  
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UniProtKB/Swiss-Prot: B4GT4_HUMAN, O60513
Pathway: Protein modification; protein glycosylation


5/21 Interacting proteins for B4GALT4 (ENSP000003521443) via UniProtKB, MINT, and/or STRING (see all 21 )

InteractantInteraction Details
GeneCardExternal ID(s)
B3GNT5ENSP000003161733STRING (score=.97)
B3GNT1ENSP000003055953STRING (score=.899)
B3GNT3ENSP000003218743STRING (score=.899)
B3GNT4ENSP000003196363STRING (score=.899)
ENSG00000174684ENSP000003090963STRING (score=.899)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005975 carbohydrate metabolic process IEA--
GO:0006643 membrane lipid metabolic process TAS9792633
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
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Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for B4GALT4

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (B4GALT4)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (B4GALT4)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_212543

              Sigma-Aldrich siRNA for B4GALT4  
                     Sigma-Aldrich shRNA for B4GALT4  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_003778  NM_212543  

REFSEQ mRNAs for B4GALT4 gene (2 alternative transcripts): 

NM_003778.3   NM_212543.1   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_003778  NM_212543  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_003778
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_003778
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_003778 

Additional cDNA sequence: 

AB024436.1 AF020920.1 AF022367.1 AF038662.1 AK001006.1 AK301688.1 AK314425.1 AY359008.1 
BC004523.2 BC062618.1 CR626075.1 CR749555.1 

20 DOTS entries:

DT.100781999  DT.91720381  DT.99944653  DT.453314  DT.120875620  DT.120875594  DT.99985352  DT.120875597 
DT.91720382  DT.120875588  DT.120875612  DT.120875599  DT.120875605  DT.120875619  DT.120875621  DT.120875583 
DT.120875593  DT.120875596  DT.120875600  DT.100692235 

24/276 AceView cDNA sequences (see all 276 ):

NM_003778 AW172525 AI926907 BP337521 AI801723 BU617040 BP364803 BP335827 
AW140052 BP336372 BP336934 AB024436 BP351505 AK001006 AA446926 CR626075 
BP353951 BP363037 BP335423 AA046963 BX359105 CB138407 BU738979 BP372960 

highest scoring ESTs for B4GALT4:

AF038662 AK001006 AU099090 AU118291 AU131994 AU280052 BI667333 BM152720 BM750470 BM754304 

Unigene Cluster for B4GALT4:

UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
Hs.13225  [show with all ESTs]
Unigene Representative Sequence: BC062618


GeneLoc Exon Structure

5/17 Alternative Splicing Database (ASD) splice patterns (SP) for B4GALT4 (see all 17 )

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b · 10c · 10d · 10e · 10f · 10g · 10h ^ 11a · 11b · 11c ^
SP1:                                                  -     -           -     -     -     -                                                                     
SP2:                                                                    -           -     -                                                                     
SP3:                                                                    -     -     -     -                                                                     
SP4:                          -     -     -           -     -           -           -     -                                                                     
SP5:                          -     -     -     -     -     -           -           -     -                                                                     

ExUns: 12a · 12b ^ 13 ^ 14 ^ 15a · 15b ^ 16
SP1:              -                 -         
SP2:              -                 -         
SP3:              -                           
SP4:                                          
SP5:                                          

About this scheme

ECgene alternative splicing isoforms for B4GALT4

2 Ensembl transcripts including schematic representations:
ENST00000393765  ENST00000359213  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

B4GALT4 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for B4GALT4

1 / 2 / 3

5 probe-sets matching B4GALT4 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
39432_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AF038662 0.80 1.00 0.91 1

212876_at2, 3 U133-A 1 1.00 1.00 -- -- BF223021 0.60 1.00 0.82 1

210540_s_at2, 3 U133-A 1 1.00 1.00 -- -- BC004523 0.60 1.00 0.82 1

212876_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

210540_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  B4GALT4 for binary patterns associated with the probe-sets selected above  
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About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AGGGTTAAAT

SOURCE GeneReport for Unigene cluster: Hs.13225

Expression variation in blood from EXPOLDB for B4GALT4

UniProtKB/Swiss-Prot: B4GT4_HUMAN, O60513
Tissue specificity: High expression in heart, placenta, kidney and pancreas; lower in brain, colon,
lung, muscle, ovary, testis and uterus

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for B4GALT4 gene from 5/10 species (see all 10 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
B4GALT41   -- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more 87.98(n)
86.34(a)
608143  XM_845063.1  XP_850156.1 
cow
(Bos taurus)
B4GALT41   -- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more 86.35(n)
83.38(a)
511328  NM_001038078.1  NP_001033167.1 
rat
(Rattus norvegicus)
B4galt41   -- UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, more 83.04(n)
83.14(a)
303923  NM_001012018.1  NP_001012018.1 
mouse
(Mus musculus)
B4galt41, 5 165
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, more1, 5 83.33(n)1
83.14(a)1
563751  NM_019804.21  NP_062778.11 
 AF1426725  AF1587465  (see all 21)
chicken
(Gallus gallus)
B4GALT41   -- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more 70.47(n)
71.47(a)
418341  XM_416563.2  XP_416563.1 
About this table        Species with no ortholog for B4GALT4

ENSEMBL Gene Tree for B4GALT4
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for B4GALT4 gene
B4GALT22  B4GALT12  B4GALT32  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/288 NCBI SNPs in B4GALT4 are shown (see all 288 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 86)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 3 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs67741,2
A,C,F,H,O120413574(-) AAAGGC/TGACGA 2 -- ut31 ese317Minor allele frequency- T:0.15MN EU EA WA NA 1772
rs38066981,2
A,C,F,H120438578(+) GAGCCG/ATGGAA 2 -- ut51 ese34Minor allele frequency- A:0.23EU EA WA 420
rs37647791,2
A,C,F,H120428486(-) GCCCTC/GAGGAA 2 Q/E mis116Minor allele frequency- N:0.00EA NA EU WA 1472
--
rs58681,2
C,F,H,O120413481(+) CAGCAC/G/TCTGCA 2 -- ut31 ese35MN EU EA WA 600
rs64385031,2
A,C,F,H120413076(+) ATGGAC/TGGGCC 2 -- ng518Minor allele frequency- N:0.00EA NA EU WA 636
rs126306901,2
C,F,H120413154(+) ACGGGA/TGACAG 2 -- ng5111Minor allele frequency- T:0.19NA EU EA WA 924
rs67842081,2
A,C120414079(+) AAAGAC/TCCAGG 2 -- ut31 ese30--------
rs45681251,2
A,C120413803(+) AATAGC/TGTTCA 2 -- ut31 ese33Minor allele frequency- T:0.01NA 142
--
rs124964201,2
C120436739(+) ATAGTG/TTTTAA 2 -- ut51 int10--------
--
rs175530531,2
F120443300(-) CCTTAC/TAGCAT 2 -- ng312Minor allele frequency- T:0.06MN EA 356
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HapMap Linkage Disequilibrium images for B4GALT4 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 604015

(Possibly Related Articles in Doctor's Guide)
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  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/17 PubMed articles for B4GALT4 gene (see all 17 ):
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 8702 HGNC: 927 AceView: B4GALT4 Ensembl:ENSG00000121578 euGenes: HUgn8702
ECgene: B4GALT4 H-InvDB: B4GALT4
(According to HUGE)
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  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
GGDBhttp://riodb.ibase.aist.go.jp/rcmg/ggdb/
Functional Glycomics Gateway - GTasehttp://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_439
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for B4GALT4:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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