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Aliases & Descriptions for B4GALT4
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases B4Gal-T4 2 Beta4Gal-T4 3 EC 2.4.1.- 3 b4Gal-T4 3 beta4Gal-T4 1 , 2
Descriptions Beta-1,4-GalTase 4 3 UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4 3 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4 2 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 2 UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4 3 beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 4 2
Search outside databases for aliases for B4GALT4 genePrevious GC identifers: GC03M115619 GC03M118559 GC03M120211 GC03M120251
Summaries for B4GALT4 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for B4GALT4 : This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type IImembrane-bound glycoproteins that appear to have exclusive specificity for the donor substrateUDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc,Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of differentglycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminalhydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remainsuncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form fourgroups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, andbeta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipidbiosynthesis. Two alternatively spliced transcript variants have been found for this gene.[provided by RefSeq] UniProtKB/Swiss-Prot: B4GT4_HUMAN, O60513 Function : Responsible for the synthesis of complex-type N-linked oligosaccharides in manyglycoproteins as well as the carbohydrate moieties of glycolipids
Gene Wiki entry for B4GALT4
Genomic Location for B4GALT4
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the B4GALT4 gene Entrez Gene cytogenetic band: 3q13.3 Ensembl cytogenetic band: 3q13.32 HGNC cytogenetic band: 3q13.3 B4GALT4 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 3 GeneLoc Exon Structure
GeneLoc location for GC03M120413:
(about GC identifiers )
Start:
120,413,279 bp from pter
End:
120,442,442 bp from pter
Size:
29,164 bases
Orientation:
minus strand
RefSeq DNA sequence: NC_000003.10 NT_005612.15 Proteins for B4GALT4
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: B4GT4_HUMAN, O60513 (See
protein sequence )Recommended Name: Beta-1,4-galactosyltransferase 4 Size : 344 amino acids; 40041 Da
Cofactor : Manganese (By similarity)
Subcellular location : Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein.Note=Trans cisternae of Golgi stack
Secondary accessions : Q68D68 Q9BSW3 Q9C078
REFSEQ proteins (2 alternative transcripts):
NP_003769.1 NP_997708.1 ENSEMBL proteins: ENSP00000377360 ENSP00000352144 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 3 Gene Ontology (GO) cellular component terms (links to tree view) :
About this table Antibodies for B4GALT4: Assays for B4GALT4:
Protein
Domains/ Families for B4GALT4(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry O60513 ProtoNet protein and cluster: O60513
1 Blocks protein family : IPB003859 Metazoa galactosyltransferase UniProtKB/Swiss-Prot: B4GT4_HUMAN, O60513 Similarity : Belongs to the glycosyltransferase 7 family
Gene Function for B4GALT4
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
OriGene ,
Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
RNAi Products ,
Clones , and
Q-PCR Products
from Invitrogen ,
Millipore ,
OriGene , and/or
Abnova ,
siRNAs from
Applied Biosystems ,
SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_212543 Applied Biosystems Silencer ® siRNAs for B4GALT4 Sigma-Aldrich siRNA for B4GALT4 Sigma-Aldrich shRNA for B4GALT4 Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_003778                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_003778                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_003778  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_212543 UniProtKB/Swiss-Prot: B4GT4_HUMAN, O60513 Function : Responsible for the synthesis of complex-type N-linked oligosaccharides in manyglycoproteins as well as the carbohydrate moieties of glycolipids Catalytic activity : UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamineBiophysicochemical properties : Kinetic parameters: KM=238 uM for GlcNAc-B-S-pNP;Enzyme Number (IUBMB): EC 2.4.1.-
Genatlas biochemistry entry for B4GALT4 :UDP galactose 5 Gene Ontology (GO) molecular function terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0003945 N-acetyllactosamine synthase activity
IEA -- GO:0008378 galactosyltransferase activity
TAS 9792633 GO:0016757 transferase activity, transferring glycosyl groups
IEA -- GO:0030145 manganese ion binding
IEA -- GO:0046872 metal ion binding
IEA --
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Pathways & Interactions for B4GALT4
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for B4GALT4 (Your Favorite Gene powered by Ingenuity) UniProtKB/Swiss-Prot: B4GT4_HUMAN, O60513 Pathway : Protein modification; protein glycosylation
5/21 Interacting proteins for B4GALT4 (ENSP00000352144 3 ) via UniProtKB, MINT, and/or STRING (see all 21
)About this table 2 Gene Ontology (GO) biological process terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0005975 carbohydrate metabolic process
IEA -- GO:0006643 membrane lipid metabolic process
TAS 9792633
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Drugs & Compounds for B4GALT4 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for B4GALT4
Transcripts for B4GALT4(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
Abnova ,
siRNAs from Applied Biosystems ,
Sigma-Aldrich ,
shRNA from
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OriGene ,
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_212543 Sigma-Aldrich siRNA for B4GALT4 Sigma-Aldrich shRNA for B4GALT4 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_003778 NM_212543
REFSEQ mRNAs for B4GALT4 gene (2 alternative transcripts): NM_003778.3 NM_212543.1
Applied Biosystems TaqMan ® Gene Expression Assays: NM_003778 NM_212543
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_003778                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_003778                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_003778  
Additional cDNA sequence: AB024436.1 AF020920.1 AF022367.1 AF038662.1 AK001006.1 AK301688.1 AK314425.1 AY359008.1 BC004523.2 BC062618.1 CR626075.1 CR749555.1
20 DOTS entries : DT.100781999 DT.91720381 DT.99944653 DT.453314 DT.120875620 DT.120875594 DT.99985352 DT.120875597 DT.91720382 DT.120875588 DT.120875612 DT.120875599 DT.120875605 DT.120875619 DT.120875621 DT.120875583 DT.120875593 DT.120875596 DT.120875600 DT.100692235
24/276 AceView cDNA sequences (see all 276
):NM_003778 AW172525 AI926907 BP337521 AI801723 BU617040 BP364803 BP335827 AW140052 BP336372 BP336934 AB024436 BP351505 AK001006 AA446926 CR626075 BP353951 BP363037 BP335423 AA046963 BX359105 CB138407 BU738979 BP372960
highest scoring ESTs for B4GALT4 :AF038662 AK001006 AU099090 AU118291 AU131994 AU280052 BI667333 BM152720 BM750470 BM754304
Unigene Cluster for B4GALT4: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 Hs.13225 [show with all ESTs ] Unigene Representative Sequence: BC062618 GeneLoc Exon Structure 5/17 Alternative Splicing Database (ASD) splice patterns (SP) for B4GALT4 (see all 17
) ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b · 10c · 10d · 10e · 10f · 10g · 10h ^ 11a · 11b · 11c ^ SP1 :                 -   -     -   -   -   -                         SP2 :                       -     -   -                         SP3 :                       -   -   -   -                         SP4 :         -   -   -     -   -     -     -   -                         SP5 :         -   -   -   -   -   -     -     -   -                        
ExUns: 12a · 12b ^ 13 ^ 14 ^ 15a · 15b ^ 16 SP1 :     -       -     SP2 :     -       -     SP3 :     -           SP4 :               SP5 :              
About this scheme ECgene alternative splicing isoforms for B4GALT4 2 Ensembl transcripts including schematic representations : ENST00000393765
ENST00000359213
Expression for B4GALT4
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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B4GALT4 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for B4GALT4 1 / 2 / 3
5 probe-sets matching B4GALT4 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: AGGGTTAAATSOURCE GeneReport for Unigene cluster: Hs.13225 Expression variation in blood from EXPOLDB for B4GALT4
UniProtKB/Swiss-Prot: B4GT4_HUMAN, O60513 Tissue specificity : High expression in heart, placenta, kidney and pancreas; lower in brain, colon,lung, muscle, ovary, testis and uterus
Orthologs for B4GALT4
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for B4GALT4 gene from 5/10 species (see all 10
)
Organism
Gene
Locus
Description
Human Similarity
NCBI accessions
dog (Canis familiaris)
B4GALT41
--
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more
87.98(n) 86.34(a)
608143 XM_845063.1 XP_850156.1
cow (Bos taurus)
B4GALT41
--
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more
86.35(n) 83.38(a)
511328 NM_001038078.1 NP_001033167.1
rat (Rattus norvegicus)
B4galt41
--
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, more
83.04(n) 83.14(a)
303923 NM_001012018.1 NP_001012018.1
mouse (Mus musculus)
B4galt41 , 5
165
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, more 1, 5
83.33(n) 1 83.14(a) 1
56375 1 NM_019804.2 1 NP_062778.1 1 AF142672 5 AF158746 5 (see all 21 )
chicken (Gallus gallus)
B4GALT41
--
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more
70.47(n) 71.47(a)
418341 XM_416563.2 XP_416563.1
About this table Species with no ortholog for B4GALT4 ENSEMBL Gene Tree for B4GALT4 Paralogs for B4GALT4 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for B4GALT4 gene B4GALT2 2 B4GALT1 2 B4GALT3 2
SNPs/Variants for B4GALT4 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for B4GALT4 (up to first 250kb)
Disorders & Mutations for B4GALT4
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 604015
Medical News for B4GALT4 (Possibly Related Articles in
Doctor's Guide )
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--
Publications for B4GALT4 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/17 PubMed articles for B4GALT4 gene (see all 17
): The expanding beta 4-galactosyltransferase gene family: messages from the databanks. (PubMed id 9597550) 2, 3, 4 Lo N.W....Shaper N.L. (1998) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 3, 4 Gerhard D.S....Malek J. (2004) The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309) 3, 4 Clark H.F....Gray A. (2003) Galactosylation of N-linked oligosaccharides by human beta-1,4- galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells. (PubMed id 11588157) 3, 4 Guo S.... Furukawa K. (2001) Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. (PubMed id 10580128) 3, 4 Amado M.... Clausen H. (1999) Cloning of a novel member of the UDP-galactose:beta-N- acetylglucosamine beta1,4-galactosyltransferase family, beta4Gal-T4, involved in glycosphingolipid biosynthesis. (PubMed id 9792633) 3, 4 Schwientek T....Clausen H. (1998) The full-ORF clone resource of the German cDNA Consortium. (PubMed id 17974005) 4 Bechtel S....Schupp I. (2007) Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039) 3 Ota T....Sugano S. (2004) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932) 3 Strausberg R.L....Marra M.A. (2002) Identification and characterization of the potential promoter regions of 1031 kinds of human genes. (PubMed id 11337467) 3 Suzuki Y....Sugano S. (2001)
Search for B4GALT4
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Genome Databases showing B4GALT4
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing B4GALT4
(According to HUGE )
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--
Specialized Databases showing B4GALT4 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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Name Description
GGDB http://riodb.ibase.aist.go.jp/rcmg/ggdb/ Functional Glycomics Gateway - GTase http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_439
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-- Services for B4GALT4 (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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Products for B4GALT4:
Search Tocris compounds for B4GALT4
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GeneCards Homepage - Last full update: 2 Jul 2009
Incremental update: 13 Oct 2009