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Aliases & Descriptions for B4GALT2
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases B4Gal-T2 2 B4Gal-T3 2 Beta4Gal-T2 3 EC 2.4.1.- 3 OTTHUMP00000010008 2 b4Gal-T2 3 beta-4-GalT2 2 beta4Gal-T2 1 , 2
Descriptions Beta-1,4-GalTase 2 3 UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2 3 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 2 2 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2 2 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 2 2 UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2 3 beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 2 2
Search outside databases for aliases for B4GALT2 genePrevious GC identifers: GC01P044283 GC01P043445 GC01P043875 GC01P043858 GC01P044114
Summaries for B4GALT2 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for B4GALT2 : This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type IImembrane-bound glycoproteins that appear to have exclusive specificity for the donor substrateUDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc,Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of differentglycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminalhydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remainsuncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form fourgroups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, andbeta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids andglycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressedin lactating mammary tissue. Two transcript variants encoding the same protein have been found forthis gene. [provided by RefSeq] UniProtKB/Swiss-Prot: B4GT2_HUMAN, O60909 Function : Responsible for the synthesis of complex-type N-linked oligosaccharides in manyglycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose
Gene Wiki entry for B4GALT2
Genomic Location for B4GALT2
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
SABiosciences )About This Section
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the B4GALT2 gene Entrez Gene cytogenetic band: 1p34-p33 Ensembl cytogenetic band: 1p34.1 HGNC cytogenetic band: 1p34-p33 B4GALT2 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 1 GeneLoc Exon Structure
GeneLoc location for GC01P044217:
(about GC identifiers )
Start:
44,217,453 bp from pter
End:
44,229,427 bp from pter
Size:
11,975 bases
Orientation:
plus strand
RefSeq DNA sequence: NC_000001.9 NT_032977.8 Proteins for B4GALT2
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: B4GT2_HUMAN, O60909 (See
protein sequence )Recommended Name: Beta-1,4-galactosyltransferase 2 Size : 372 amino acids; 41972 Da
Cofactor : Manganese (By similarity)
Subcellular location : Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein.Note=Trans cisternae of Golgi stack
Secondary accessions : O60511 Q4V9L9 Q5T4X5 Q5T4Y5 Q9BUP6 Q9NSY7Alternative splicing : 2 isoforms : O60909-1 O60909-2
REFSEQ proteins (2 alternative transcripts):
NP_001005417.1 NP_003771.1 ENSEMBL proteins: ENSP00000310696 ENSP00000349293 ENSP00000361399 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 3 Gene Ontology (GO) cellular component terms (links to tree view) :
About this table Antibodies for B4GALT2: Assays for B4GALT2:
Protein
Domains/ Families for B4GALT2(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry O60909 ProtoNet protein and cluster: O60909
1 Blocks protein family : IPB003859 Metazoa galactosyltransferase UniProtKB/Swiss-Prot: B4GT2_HUMAN, O60909 Similarity : Belongs to the glycosyltransferase 7 family
Gene Function for B4GALT2
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
OriGene ,
Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
RNAi Products ,
Clones , and
Q-PCR Products
from Invitrogen ,
Millipore ,
OriGene , and/or
Abnova ,
siRNAs from
Applied Biosystems ,
SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3 ): NM_003780 Applied Biosystems Silencer ® siRNAs for B4GALT2 Sigma-Aldrich siRNA for B4GALT2 Sigma-Aldrich shRNA for B4GALT2 Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_001005417                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_001005417                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_001005417  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_001005417 UniProtKB/Swiss-Prot: B4GT2_HUMAN, O60909 Function : Responsible for the synthesis of complex-type N-linked oligosaccharides in manyglycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose Catalytic activity : UDP-galactose + D-glucose = UDP + lactoseCatalytic activity : UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP +beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide Catalytic activity : UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamineBiophysicochemical properties : Kinetic parameters: KM=71 uM for GlcNAc-B-S-pNP;Enzyme Number (IUBMB): EC 2.4.1.-
Genatlas biochemistry entry for B4GALT2 :UDP galactose 5/7 Gene Ontology (GO) molecular function terms (links to tree view) (see all 7
):
GO ID Qualified GO term Evidence PubMed IDs GO:0003831 beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity
IEA -- GO:0003945 N-acetyllactosamine synthase activity
IEA -- GO:0004461 lactose synthase activity
IEA -- GO:0008378 galactosyltransferase activity
TAS 9405390 GO:0016757 transferase activity, transferring glycosyl groups
IEA --
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Pathways & Interactions for B4GALT2
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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3 Sigma-Aldrich "Your Favorite Gene" Pathways for B4GALT2 (Your Favorite Gene powered by Ingenuity) UniProtKB/Swiss-Prot: B4GT2_HUMAN, O60909 Pathway : Protein modification; protein glycosylation
5/24 Interacting proteins for B4GALT2 (ENSP00000361399 3 ) via UniProtKB, MINT, and/or STRING (see all 24
)About this table 1 Gene Ontology (GO) biological process term (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0005975 carbohydrate metabolic process
IEA --
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Drugs & Compounds for B4GALT2 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
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Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for B4GALT2
Transcripts for B4GALT2(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
Abnova ,
siRNAs from Applied Biosystems ,
Sigma-Aldrich ,
shRNA from
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OriGene ,
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3 ): NM_003780 Sigma-Aldrich siRNA for B4GALT2 Sigma-Aldrich shRNA for B4GALT2 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_001005417 NM_003780
REFSEQ mRNAs for B4GALT2 gene (2 alternative transcripts): NM_001005417.1 NM_003780.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_001005417 NM_003780
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_001005417                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_001005417                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_001005417  
Additional cDNA sequence: AB024434.1 AF038660.1 AK074533.1 AK095873.1 AK293418.1 AL137647.1 BC002431.2 BC096821.1 CR591692.1 CR607401.1 Y12510.1
18 DOTS entries : DT.443441 DT.75103445 DT.86855255 DT.75193620 DT.101954755 DT.424518 DT.95200940 DT.97812595 DT.424564 DT.86855254 DT.91769702 DT.100820346 DT.100820347 DT.100820349 DT.75103444 DT.101973129 DT.92061753 DT.99937626
24/241 AceView cDNA sequences (see all 241
):BQ684720 AI950908 Y12510 AI361763 BM772137 BU931196 AI150669 AL138200 CB153157 CR607401 AL541643 CR591692 BQ646141 BQ654304 AW136464 AW276110 CD675528 BM756950 BM794222 AW082085 BM794874 BM852314 BU192652 BQ691759
highest scoring ESTs for B4GALT2 :AF038660 AA410679 AA452865 AA577473 AA586957 AA587007 AA626048 AA780213 AA954087 AB024434
Unigene Cluster for B4GALT2: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2 Hs.632403 [show with all ESTs ] Unigene Representative Sequence: AK095873 GeneLoc Exon Structure 5/6 Alternative Splicing Database (ASD) splice patterns (SP) for B4GALT2 (see all 6
) ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b · 6c ^ 7a · 7b · 7c ^ 8 ^ 9a · 9b ^ 10a · 10b SP1 :           -                   -       SP2 :           -   -                 -       SP3 :   -         -                         SP4 :                                   SP5 :                                  
About this scheme ECgene alternative splicing isoforms for B4GALT2 3 Ensembl transcripts including schematic representations : ENST00000309519
ENST00000356836
ENST00000372324
Expression for B4GALT2
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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B4GALT2 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for B4GALT2 1 / 2 / 3
4 probe-sets matching B4GALT2 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: GGAGGCAGGTSOURCE GeneReport for Unigene cluster: Hs.632403 Expression variation in blood from EXPOLDB for B4GALT2
UniProtKB/Swiss-Prot: B4GT2_HUMAN, O60909 Tissue specificity : Weakly expressed in various tissues. Highest expression in prostate, testis,ovary, intestine, muscle, and in fetal brain
Orthologs for B4GALT2
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for B4GALT2 gene from 5/9 species (see all 9
)
Organism
Gene
Locus
Description
Human Similarity
NCBI accessions
dog (Canis familiaris)
B4GALT21
--
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more
92.5(n) 97.02(a)
482527 XM_539644.2 XP_539644.1
rat (Rattus norvegicus)
B4galt21
--
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more
89.25(n) 92.14(a)
313536 XM_242992.4 XP_242992.2
mouse (Mus musculus)
B4galt21 , 5
45
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more 1, 5
89.97(n) 1 92.68(a) 1
53418 1 NM_017377.4 1 NP_059073.1 1 AB019541 5 AF142670 5 (see all 17 )
chicken (Gallus gallus)
B4GALT21
--
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, more
75.35(n) 78.12(a)
396122 NM_205203.1 NP_990534.1
zebrafish (Danio rerio)
CH211-261P7.41
--
novel protein similar to vertebrate UDP-Gal:betaGlcNAc more
69.11(n) 71(a)
100148828 XM_001923417.1 XP_001923452.1
About this table Species with no ortholog for B4GALT2 ENSEMBL Gene Tree for B4GALT2 Paralogs for B4GALT2 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for B4GALT2 gene B4GALT4 2 B4GALT1 2 B4GALT3 2
SNPs/Variants for B4GALT2 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for B4GALT2 (up to first 250kb)
Disorders & Mutations for B4GALT2
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 604013
Medical News for B4GALT2 (Possibly Related Articles in
Doctor's Guide )
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--
Publications for B4GALT2 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/18 PubMed articles for B4GALT2 gene (see all 18
): A family of human beta4-galactosyltransferases. Cloning and expression of two novel UDP-galactose:beta-n-acetylglucosamine beta1, 4-galactosyltransferases, beta4Gal-T2 and beta4Gal-T3. (PubMed id 9405390) 1, 2, 3, 4 Almeida R.... Clausen H. (1997) The expanding beta 4-galactosyltransferase gene family: messages from the databanks. (PubMed id 9597550) 2, 3, 4 Lo N.W....Shaper N.L. (1998) The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414) 3, 4 Gregory S.G.... Bentley D.R. (2006) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 3, 4 Gerhard D.S....Malek J. (2004) Galactosylation of N-linked oligosaccharides by human beta-1,4- galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells. (PubMed id 11588157) 3, 4 Guo S.... Furukawa K. (2001) Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. (PubMed id 10580128) 3, 4 Amado M.... Clausen H. (1999) Identification of {beta}1,4GalT II as a Target Gene of p53-mediated HeLa Cell Apoptosis. (PubMed id 18211920) 3 Zhou J....Gu J. (2008) beta4GalT-II increases cisplatin-induced apoptosis in HeLa cells depending on its Golgi localization. (PubMed id 17470362) 3 Jiang J....Gu J. (2007) The full-ORF clone resource of the German cDNA Consortium. (PubMed id 17974005) 4 Bechtel S....Schupp I. (2007) beta4GalT-II is a key regulator of glycosylation of the proteins involved in neuronal development. (PubMed id 15939404) 3 Sasaki N....Nishihara S. (2005)
Search for B4GALT2
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AND OR
Aliases
B4GALT2 (Gene Symbol) B4Gal-T2 B4Gal-T3 Beta4Gal-T2 EC 2.4.1.- OTTHUMP00000010008 b4Gal-T2 beta-4-GalT2 beta4Gal-T2 Beta-1,4-GalTase 2 UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 2 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 2 UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2 beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 2
Free Text
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OMIM
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Genome Databases showing B4GALT2
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing B4GALT2
(According to HUGE )
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Specialized Databases showing B4GALT2 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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Name Description
GGDB http://riodb.ibase.aist.go.jp/rcmg/ggdb/ Functional Glycomics Gateway - GTase http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_437
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-- Services for B4GALT2 (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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Products for B4GALT2:
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GeneCards Homepage - Last full update: 2 Jul 2009
Incremental update: 13 Oct 2009