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B3GNT8 Gene

protein-coding GIFtS: 44

GC19M046626

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8
(Previous name: UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 7 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: B3GALT7)

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
B3GALT7 ^², ³
BGALT15 ^¹, ², ³
BGnT-8 ^³
Beta-1,3-N-acetylglucosaminyltransferase-8 ^³
Beta3Gn-T8 ^³
EC 2.4.1.- ^³
beta3Gn-T8 ^¹, ²

Descriptions
UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 7 ^¹, ²
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 ^²
beta galactosyltransferase BGALT15 ^²
beta1,3-N-acetylglucosaminyltransferase 8 ^²

External Ids
HGNC: 24139¹
Entrez Gene: 374907²
UniProtKB: Q7Z7M8³
Ensembl: ENSG00000177191⁷

Search outside databases for aliases for B3GNT8 gene

Previous GC identifer: GC00U909458

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: B3GN8_HUMAN, Q7Z7M8

Function: Beta-1,3-N-acetylglucosaminyltransferase that plays a role in the elongation of specific

branch structures of multiantennary N-glycans. Has strong activity towards tetraantennary

N-glycans and 2,6 triantennary glycans

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the B3GNT8 gene

Entrez Gene cytogenetic band: 19q13.2 Ensembl cytogenetic band: 19q13.2 HGNC cytogenetic band: 19q13

B3GNT8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19 GeneLoc Exon Structure

GeneLoc location for GC19M046626: (about GC identifiers)

Start:	46,623,104 bp from pter
End:	46,626,475 bp from pter
Size:	3,372 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000019.8 NT_011109.15

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: B3GN8_HUMAN, Q7Z7M8 (See protein sequence)

Recommended Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8

Size: 397 amino acids; 43396 Da

Subunit: Forms a complex of enhanced enzymatic activity with B3GNT2 in vitro

Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein (By

similarity)

REFSEQ proteins: NP_940942.1

ENSEMBL proteins:

ENSP00000312700

Human Recombinant Proteins

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Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000139	Golgi membrane	IEA	--
GO:0005794	Golgi apparatus	IEA	--
GO:0016020	membrane	IEA	--
GO:0016021	integral to membrane	IEA	--

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Antibodies for B3GNT8:

	Browse Antibodies Central at Invitrogen
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	Search Novus for antibodies for B3GNT8

Assays for B3GNT8:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q7Z7M8

ProtoNet protein and cluster: Q7Z7M8

1 Blocks protein family: IPB002659 Glycosyl transferase

UniProtKB/Swiss-Prot: B3GN8_HUMAN, Q7Z7M8

Similarity: Belongs to the glycosyltransferase 31 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (B3GNT8)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_198540

Applied Biosystems Silencer® siRNAs for B3GNT8

Sigma-Aldrich siRNA for B3GNT8

Sigma-Aldrich shRNA for B3GNT8

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_198540
Myc/DDK tagged cDNA clone in CMV expression vector: NM_198540
untagged cDNA clone in CMV expression vector: NM_198540

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_198540

UniProtKB/Swiss-Prot: B3GN8_HUMAN, Q7Z7M8

Function: Beta-1,3-N-acetylglucosaminyltransferase that plays a role in the elongation of specific

branch structures of multiantennary N-glycans. Has strong activity towards tetraantennary

N-glycans and 2,6 triantennary glycans

Induction: Up-regulated in colon cancer

Enzyme Number (IUBMB): EC 2.4.1.-

1 MGI phenotypic allele for B3gnt8 (no phenotypes)

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008378	galactosyltransferase activity	IEA	--
GO:0016262	protein N-acetylglucosaminyltransferase activity	IDA	15620693
GO:0016757	transferase activity, transferring glycosyl groups	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

UniProtKB/Swiss-Prot: B3GN8_HUMAN, Q7Z7M8

Pathway: Protein modification; protein glycosylation

2 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006486	protein amino acid glycosylation	IEA	--
GO:0030311	poly-N-acetyllactosamine biosynthetic process	IDA	15620693

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for B3GNT8

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (B3GNT8)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_198540

Sigma-Aldrich siRNA for B3GNT8

Sigma-Aldrich shRNA for B3GNT8

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_198540

REFSEQ mRNAs for B3GNT8 gene:

NM_198540.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_198540

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_198540
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_198540
                                 untagged cDNA clone in CMV expression vector: NM_198540

Additional cDNA sequence:

AB175895.1 AJ419172.1 AY277592.2

2 DOTS entries:

DT.70102433 DT.99980291

19 AceView cDNA sequences:

BQ774493 NM_198540 AY277592 AJ419172 CA433805 BX105512 BM726913 CA431056

AI760623 BF064085 CA418485 BM478343 BM771654 AW444713 BV191920 AI917642

AI492018 AI347343 BM992860

highest scoring ESTs for B3GNT8:

AI760623 AY277592 AI492018 AJ419172 AW444713 BF064085 BM478343 BM726913 BM771654 BM992860

Unigene Cluster for B3GNT8:

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8

Hs.441681 [show with all ESTs]

Unigene Representative Sequence: AY277592

GeneLoc Exon Structure

2 Alternative Splicing Database (ASD) splice patterns (SP) for B3GNT8

ExUns:	1a	·	1b	^	2a	·	2b	·	2c
SP1:
SP2:					-

About this scheme

ECgene alternative splicing isoforms for B3GNT8

1 Ensembl transcript including schematic representation:

ENST00000321702

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

B3GNT8 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for B3GNT8

¹ / ² / ³

3 probe-sets matching B3GNT8 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
78826_at²^{, 3}	U95-E	1	1.00	1.00	1.00	1.00	AI760623	0.80	1.00	0.91	1
237338_at²^{, 3}	U133-B	1	1.00	1.00	--	--	AI760623	0.80	1.00	0.91	1
237338_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks B3GNT8 for binary patterns associated with the probe-sets selected above

About GeneDecksing

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: CACTCCCCAC

SOURCE GeneReport for Unigene cluster: Hs.441681
UniProtKB/Swiss-Prot: B3GN8_HUMAN, Q7Z7M8
Tissue specificity: Highly expressed in small intestine, pancreas, spleen, bone marrow, lung,
throat, and ileum, and weakly in fetal brain, cerebellum, heart, liver, tongue, breast, uteri, and
testis. Not detected in colon. Differentially expressed in human tumor cell lines

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for B3GNT8 gene from 5 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	B3GNT8¹	--	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more	84.78(n) 84.17(a)	484487 XM_541601.2 XP_541601.2
chimpanzee (Pan troglodytes)	B3GNT8¹	--	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more	99.5(n) 99.24(a)	468890 XM_524276.2 XP_524276.1
cow (Bos taurus)	B3GNT8¹	--	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more	85.47(n) 84.13(a)	534859 NM_001076040.1 NP_001069508.1
rat (Rattus norvegicus)	B3gnt8¹	--	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more	80.81(n) 77.89(a)	308440 XM_218342.4 XP_218342.3
mouse (Mus musculus)	B3gnt8^{1, 5}	7⁵	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more^{1, 5}	80.38(n)¹ 77.63(a)¹	232984¹ NM_146184.4¹ NP_666296.1¹ AK042388⁵ AK154220⁵ (see all 10)

About this table Species with no ortholog for B3GNT8

ENSEMBL Gene Tree for B3GNT8

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for B3GNT8 gene

B3GNT1²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/41 NCBI SNPs in B3GNT8 are shown (see all 41 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 58)

AB	SNP ID	Valid	Chr 19 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs284662^1,2	C,F,H	46624115(-)	`GTGGCG/AGCCAA`	1	G/S	mis¹ ese³		7		EU EA WA NA	546
--	rs8102142^1,2	A,C	46627574(+)	`GGtttC/Tttttt`	1	--	ng3¹ trp³		0	--	--	--	--
--	rs2569400^1,2	A,C	46628448(-)	`gtagaG/Tacagg`	1	--	ng3¹		0	--	--	--	--
	rs7249410^1,2	H	46624436(+)	`GAGCCG/AGGACT`	1	W/R	mis¹ ese³		4		EU EA WA	416
	rs284663^1,2	A,C,F,H,O	46624452(-)	`ATCGAA/GTGGAC`	1	E/E	syn¹ ese³		7		EU EA WA NA	554
	rs284661^1,2	A,C,F,H	46623960(-)	`CCGGTG/AGGTGA`	1	V/V	syn¹ ese³		7		EU EA WA NA	542
	rs284660^1,2	A,C,F,H	46623924(-)	`GTGGCC/ATGGGA`	1	A/A	syn¹ ese³		8		EU EA WA NA	2034
	rs7248964^1,2	F,H	46624299(+)	`TGCTGC/TTGGTT`	1	Q/Q	syn¹ ese³		4		EU EA WA	408
	rs11878656^1,2	H	46623719(+)	`GCTTCG/TGGCCG`	1	R/R	syn¹ ese³		4		EU EA WA	420
	rs10421104^1,2	F,H	46625890(+)	`GGACTC/TAGGCT`	1	--	int¹		4		EU EA WA	418

About this table

HapMap Linkage Disequilibrium images for B3GNT8 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

4 PubMed articles for B3GNT8 gene:

Cloning and tissue distribution of the human B3GALT7 gene, a member of the beta1,3-Glycosyltransferase family. (PubMed id 15486459)^{2, 3, 4} Huang C.... Yu L. (2004)
A novel beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T8), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer. (PubMed id 15620693)^{1, 3, 4} Ishida H....Narimatsu H. (2005)
Activation of beta1,3-N-acetylglucosaminyltransferase-2 (beta3Gn-T2) by beta3Gn-T8. Possible involvement of beta3Gn-T8 in increasing poly-N-acetyllactosamine chains in differentiated HL-60 cells. (PubMed id 18826941)³ Seko A. and Yamashita K. (2008)
Characterization of a novel galactose beta1,3-N- acetylglucosaminyltransferase (beta3Gn-T8): the complex formation of beta3Gn-T2 and beta3Gn-T8 enhances enzymatic activity. (PubMed id 15917431)⁴ Seko A. and Yamashita K. (2005)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 374907	HGNC: 24139	AceView: B3GALT7	Ensembl:ENSG00000177191	euGenes: HUgn374907
ECgene: B3GNT8	H-InvDB: B3GNT8

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
GGDB	http://riodb.ibase.aist.go.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=B3GNT8

Licensable Technologies for B3GNT8
(Available from WIS Yeda, Salk, Tufts)

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(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
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Enzo Life Sciences, and/or Tocris Bioscience)
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Products for B3GNT8:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

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Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for B3GNT8-related products

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B3GNT8 Gene

protein-coding GIFtS: 44

Aliases &
Descriptions
for B3GNT8

Summaries
for B3GNT8

Genomic Location
for B3GNT8

Proteins
for B3GNT8

Protein Domains/
Families
for B3GNT8

Gene Function
for B3GNT8

Pathways & Interactions
for B3GNT8

Drugs & Compounds
for B3GNT8

Transcripts
for B3GNT8

Expression
for B3GNT8

Orthologs
for B3GNT8

Paralogs
for B3GNT8

SNPs/Variants
for B3GNT8

Disorders & Mutations
for B3GNT8

Medical News
for B3GNT8

Publications
for B3GNT8

Search for B3GNT8

Genome Databases showing B3GNT8

Other Databases showing B3GNT8

Specialized Databases showing B3GNT8

Licensable Technologies
for B3GNT8

Services
for B3GNT8

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

B3GNT8 Gene

protein-coding GIFtS: 44

Aliases & Descriptionsfor B3GNT8

Summariesfor B3GNT8

Genomic Locationfor B3GNT8

Proteinsfor B3GNT8

Protein Domains/Familiesfor B3GNT8

Gene Functionfor B3GNT8

Pathways & Interactionsfor B3GNT8

Drugs & Compoundsfor B3GNT8

Transcriptsfor B3GNT8

Expression for B3GNT8

Orthologsfor B3GNT8

Paralogsfor B3GNT8

SNPs/Variantsfor B3GNT8

Disorders & Mutationsfor B3GNT8

Medical Newsfor B3GNT8

Publicationsfor B3GNT8

Search for B3GNT8

Genome Databases showing B3GNT8

Other Databases showing B3GNT8

Specialized Databases showing B3GNT8

Licensable Technologiesfor B3GNT8

Servicesfor B3GNT8

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for B3GNT8

Summaries
for B3GNT8

Genomic Location
for B3GNT8

Proteins
for B3GNT8

Protein Domains/
Families
for B3GNT8

Gene Function
for B3GNT8

Pathways & Interactions
for B3GNT8

Drugs & Compounds
for B3GNT8

Transcripts
for B3GNT8

Expression
for B3GNT8

Orthologs
for B3GNT8

Paralogs
for B3GNT8

SNPs/Variants
for B3GNT8

Disorders & Mutations
for B3GNT8

Medical News
for B3GNT8

Publications
for B3GNT8

Licensable Technologies
for B3GNT8

Services
for B3GNT8