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B3GNT7 Gene

protein-coding GIFtS: 46

GC02P231968

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
BGnT-7 ^³
Beta-1,3-N-acetylglucosaminyltransferase-7 ^³
Beta3Gn-T7 ^³
EC 2.4.1.- ^³
beta3GnT7 ^¹, ²

Descriptions
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7 ^²
beta 1,3-N-acetylglucosaminyltransferase 7 ^²

External Ids
HGNC: 18811¹
Entrez Gene: 93010²
UniProtKB: Q8NFL0³
Ensembl: ENSG00000156966⁷

Search outside databases for aliases for B3GNT7 gene

Previous GC identifers: GC02P230320 GC02P230992 GC02P232225 GC02P232462 GC02P232085

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: B3GN7_HUMAN, Q8NFL0

Function: May be involved in keratane sulfate biosynthesis. Transfers N-acetylgalactosamine on to

keratan sulfate-related glycans. May play a role in preventing cells from migrating out of the

original tissues and invading surrounding tissues

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the B3GNT7 gene

Entrez Gene cytogenetic band: 2q37.1 Ensembl cytogenetic band: 2q37.1 HGNC cytogenetic band: 2q36.1

B3GNT7 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2 GeneLoc Exon Structure

GeneLoc location for GC02P231968: (about GC identifiers)

Start:	231,953,837 bp from pter
End:	231,974,119 bp from pter
Size:	20,283 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000002.10 NT_005403.16

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: B3GN7_HUMAN, Q8NFL0 (See protein sequence)

Recommended Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7

Size: 401 amino acids; 45987 Da

Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein (Potential)

Secondary accessions: B3KWY4

REFSEQ proteins: NP_660279.1

ENSEMBL proteins:

ENSP00000287590 ENSP00000386905

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (B3GNT7)
	Human Recombinant Proteins from Abnova (B3GNT7)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000139	Golgi membrane	IEA	--
GO:0005794	Golgi apparatus	IEA	--
GO:0016020	membrane	IEA	--
GO:0016021	integral to membrane	IEA	--

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Antibodies for B3GNT7:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for B3GNT7
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (B3GNT7), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (B3GNT7)
	Novus Biologicals Antibody for B3GNT7

Assays for B3GNT7:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry Q8NFL0

ProtoNet protein and cluster: Q8NFL0

1 Blocks protein family: IPB002659 Glycosyl transferase

UniProtKB/Swiss-Prot: B3GN7_HUMAN, Q8NFL0

Similarity: Belongs to the glycosyltransferase 31 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (B3GNT7)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_145236

Applied Biosystems Silencer® siRNAs for B3GNT7

Sigma-Aldrich siRNA for B3GNT7

Sigma-Aldrich shRNA for B3GNT7

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_145236
Myc/DDK tagged cDNA clone in CMV expression vector: NM_145236
untagged cDNA clone in CMV expression vector: NM_145236

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_145236

UniProtKB/Swiss-Prot: B3GN7_HUMAN, Q8NFL0

Function: May be involved in keratane sulfate biosynthesis. Transfers N-acetylgalactosamine on to

keratan sulfate-related glycans. May play a role in preventing cells from migrating out of the

original tissues and invading surrounding tissues

Enzyme Number (IUBMB): EC 2.4.1.-

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008378	galactosyltransferase activity	IEA	--
GO:0016757	transferase activity, transferring glycosyl groups	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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UniProtKB/Swiss-Prot: B3GN7_HUMAN, Q8NFL0

Pathway: Protein modification; protein glycosylation

1 Gene Ontology (GO) biological process term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006486	protein amino acid glycosylation	IEA	--

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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for B3GNT7

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (B3GNT7)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_145236

Sigma-Aldrich siRNA for B3GNT7

Sigma-Aldrich shRNA for B3GNT7

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_145236

REFSEQ mRNAs for B3GNT7 gene:

NM_145236.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_145236

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_145236
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_145236
                                 untagged cDNA clone in CMV expression vector: NM_145236

Additional cDNA sequence:

AF502430.1 AK000770.1 AK126207.1 CR626252.1

2 DOTS entries:

DT.95182937 DT.121035174

24/41 AceView cDNA sequences (see all 41 ):

AW591607 AK126207 NM_145236 AA669325 AF502430 CR626252 CA310615 AW468484

BG830051 CA310256 CA312028 BX327708 BG832264 CA314028 AK000770 BQ448611

AA568650 AA296727 CA310801 BU686873 AW963021 BQ705935 BQ446723 BQ962194

highest scoring ESTs for B3GNT7:

AF502430 AK126207 BG830051 BG832264 BQ962194 AK000770 AW862396 BP351761 BX327708 BX642147

Unigene Cluster for B3GNT7:

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7

Hs.299329 [show with all ESTs]

Unigene Representative Sequence: AK126207

GeneLoc Exon Structure

2 Ensembl transcripts including schematic representations:

ENST00000287590 ENST00000409305

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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B3GNT7 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for B3GNT7

¹ / ² / ³

3 probe-sets matching B3GNT7 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
1555963_x_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
1552965_a_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
1555962_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: TGCCTGGAGG

SOURCE GeneReport for Unigene cluster: Hs.299329

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for B3GNT7 gene from 5/8 species (see all 8 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	B3GNT7¹	--	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more	89.25(n) 89.17(a)	486158 XM_543284.2 XP_543284.2
cow (Bos taurus)	B3GNT7¹	--	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more	85.47(n) 84.13(a)	538799 XM_582813.3 XP_582813.3
rat (Rattus norvegicus)	B3gnt7¹	--	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more	82.28(n) 85.64(a)	316583 NM_001012134.1 NP_001012134.1
mouse (Mus musculus)	B3gnt7^{1, 5}	1⁵	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more^{1, 5}	82.12(n)¹ 85.64(a)¹	227327¹ NM_145222.1¹ NP_660257.1¹ AA611902⁵ AF502429⁵ (see all 19)
chicken (Gallus gallus)	B3GNT7¹	--	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more	69.51(n) 68.09(a)	424930 XM_422738.2 XP_422738.1

About this table Species with no ortholog for B3GNT7

ENSEMBL Gene Tree for B3GNT7

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for B3GNT7 gene

B3GNT3² B3GNT4²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/15 NCBI SNPs in B3GNT7 are shown (see all 15 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 15)

AB	SNP ID	Valid	Chr 2 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs10445830^1,2	A,C,F,H	231972217(+)	`AGGAAC/TGCTGT`	1	--	ng3¹		4		EU EA WA	406
	rs2290130^1,2	C,F,H	231971371(-)	`GGGGAC/TGTGGG`	1	V/I	mis¹ ese³		12		EA NA EU WA	2444
	rs17513198^1,2	C,F,H	231967620(+)	`AGTGAG/ACCAGT`	1	--	ng5¹		11		NA EU EA WA	980
	rs11692601^1,2	C,F,H	231967763(+)	`GAAACC/TTCAAC`	1	--	ng5¹		8		EU EA WA	834
	rs13424706^1,2	H	231967144(+)	`GAGGGC/GCATTA`	1	--	ng5¹		4		EU EA WA	418
--	rs16828037^1,2	F	231966762(+)	`CCAACG/ACCCAT`	1	--	ng5¹		3		NA	140
	rs10445829^1,2	C,F,H	231970663(+)	`CCTCTG/ATACTG`	1	--	int¹		8		EU EA WA	836
	rs6750321^1,2	C,F,H	231970032(+)	`TCAACC/AATGAG`	1	--	int¹		4		EU EA WA	418
	rs13004183^1,2	H	231970245(+)	`CCGCAG/TGGAAA`	1	--	int¹		4		EU EA WA	416
--	rs35959531^1,2	--	231968508(+)	`GGGAG-/CCTAGG`	1	--	ng5¹		0	--	--	--	--

About this table

HapMap Linkage Disequilibrium images for B3GNT7 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
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5 PubMed articles for B3GNT7 gene:

A novel beta1,3-N-acetylglucosaminyltransferase involved in invasion of cancer cells as assayed in vitro. (PubMed id 12061784)^{2, 3, 4} Kataoka K. and Huh N.-H. (2002)
Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)^{3, 4} Hillier L.W....Wilson R.K. (2005)
Beta1,3-N-acetylglucosaminyltransferase-7 (beta3Gn-T7) acts efficiently on keratan sulfate-related glycans. (PubMed id 14706853)^{3, 4} Seko A. and Yamashita K. (2004)
Enzymes responsible for synthesis of corneal keratan sulfate glycosaminoglycans. (PubMed id 17690104)³ Kitayama K....Akama T.O. (2007)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)⁴ Ota T....Sugano S. (2004)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 93010	HGNC: 18811	AceView: B3GNT7	Ensembl:ENSG00000156966	euGenes: HUgn93010
ECgene: B3GNT7	H-InvDB: B3GNT7

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
GGDB	http://riodb.ibase.aist.go.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=B3GNT7
Functional Glycomics Gateway - GTase	http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_562

Licensable Technologies for B3GNT7
(Available from WIS Yeda, Salk, Tufts)

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(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for B3GNT7:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for B3GNT7-related products

	Millipore Custom Antibody & Bulk Services
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	Millipore Target Screening & Profiling Services

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	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
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B3GNT7 Gene

protein-coding GIFtS: 46

Aliases &
Descriptions
for B3GNT7

Summaries
for B3GNT7

Genomic Location
for B3GNT7

Proteins
for B3GNT7

Protein Domains/
Families
for B3GNT7

Gene Function
for B3GNT7

Pathways & Interactions
for B3GNT7

Drugs & Compounds
for B3GNT7

Transcripts
for B3GNT7

Expression
for B3GNT7

Orthologs
for B3GNT7

Paralogs
for B3GNT7

SNPs/Variants
for B3GNT7

Disorders & Mutations
for B3GNT7

Medical News
for B3GNT7

Publications
for B3GNT7

Search for B3GNT7

Genome Databases showing B3GNT7

Other Databases showing B3GNT7

Specialized Databases showing B3GNT7

Licensable Technologies
for B3GNT7

Services
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Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

B3GNT7 Gene

protein-coding GIFtS: 46

Aliases & Descriptionsfor B3GNT7

Summariesfor B3GNT7

Genomic Locationfor B3GNT7

Proteinsfor B3GNT7

Protein Domains/Familiesfor B3GNT7

Gene Functionfor B3GNT7

Pathways & Interactionsfor B3GNT7

Drugs & Compoundsfor B3GNT7

Transcriptsfor B3GNT7

Expression for B3GNT7

Orthologsfor B3GNT7

Paralogsfor B3GNT7

SNPs/Variantsfor B3GNT7

Disorders & Mutationsfor B3GNT7

Medical Newsfor B3GNT7

Publicationsfor B3GNT7

Search for B3GNT7

Genome Databases showing B3GNT7

Other Databases showing B3GNT7

Specialized Databases showing B3GNT7

Licensable Technologiesfor B3GNT7

Servicesfor B3GNT7

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for B3GNT7

Summaries
for B3GNT7

Genomic Location
for B3GNT7

Proteins
for B3GNT7

Protein Domains/
Families
for B3GNT7

Gene Function
for B3GNT7

Pathways & Interactions
for B3GNT7

Drugs & Compounds
for B3GNT7

Transcripts
for B3GNT7

Expression
for B3GNT7

Orthologs
for B3GNT7

Paralogs
for B3GNT7

SNPs/Variants
for B3GNT7

Disorders & Mutations
for B3GNT7

Medical News
for B3GNT7

Publications
for B3GNT7

Licensable Technologies
for B3GNT7

Services
for B3GNT7