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Aliases & Descriptions for B3GNT5
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| Aliases |
|---|
| B3GN-T5 1, 2 | | BGnT-5 3 | | Beta-1,3-N-acetylglucosaminyltransferase-5 3 | | Beta3Gn-T5 3 | | EC 2.4.1.- 3 | | beta3Gn-T5 1, 2 |
| | | Descriptions |
|---|
| Lactotriaosylceramide synthase 3 | | Lc(3)Cer synthase 3 | | Lc3 synthase 3 | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 2 | | beta 1,3 N-acetyglucosaminyltransferase Lc3 synthase 2 | | beta-1,3-N-acetylglucosaminyltransferase bGnT-5 2 |
|
| | Search outside databases for aliases for B3GNT5 genePrevious GC identifers: GC03P179760 GC03P183826 GC03P184373 GC03P184291 |
Summaries for B3GNT5(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for B3GNT5: This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids. [provided by RefSeq] UniProtKB/Swiss-Prot: B3GN5_HUMAN, Q9BYG0Function: Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Probably plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development |
Genomic Location for B3GNT5
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the B3GNT5 gene 
Entrez Gene cytogenetic band: 3q28 Ensembl cytogenetic band: 3q27.1 HGNC cytogenetic band: 3q28B3GNT5 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 3 GeneLoc Exon Structure GeneLoc location for GC03P184453:
(about GC identifiers)
Start:
|
184,453,726 bp from pter |
End:
|
184,473,873 bp from pter |
Size:
|
20,148 bases |
Orientation:
|
plus strand |
RefSeq DNA sequence:- NC_000003.10 NT_005612.15
| Proteins for B3GNT5
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: B3GN5_HUMAN, Q9BYG0 (See
protein sequence)Recommended Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 Size: 378 amino acids; 44053 Da
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein (By similarity)
Sequence caution: Sequence=BAD92754.1; Type=Frameshift; Positions=347;
Secondary accessions: Q59FE3 Q7L9Z5 Q8WWP9REFSEQ proteins: NP_114436.1
ENSEMBL proteins: ENSP00000316173
Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 
5 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for B3GNT5: Assays for B3GNT5: | Protein
Domains/ Families for B3GNT5(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry Q9BYG0
ProtoNet protein and cluster: Q9BYG0 1 Blocks protein family: IPB002659 Glycosyl transferase
UniProtKB/Swiss-Prot: B3GN5_HUMAN, Q9BYG0Similarity: Belongs to the glycosyltransferase 31 family | Gene Function for B3GNT5
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_032047
Applied Biosystems Silencer® siRNAs for B3GNT5
Sigma-Aldrich siRNA for B3GNT5  Sigma-Aldrich shRNA for B3GNT5  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_032047                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_032047                                  untagged cDNA clones in CMV expression vector (see all 2): NM_032047 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_032047
UniProtKB/Swiss-Prot: B3GN5_HUMAN, Q9BYG0Function: Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Probably plays a central role in regulating neolacto-series glycolipid synthesis during embryonic developmentInduction: Up-regulated by stimulation with retinoic acid and down-regulated with 12-O-tetradecanoylphorbol-13-acetateEnzyme Number (IUBMB): EC 2.4.1.- 1 MGI mutant phenotype (inferred from 1 allele ) (MGI details for B3gnt5):
4 Gene Ontology (GO) molecular function terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0008378 | galactosyltransferase activity |
IEA | -- | | GO:0008457 | beta-galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,3-acetylglucosaminyltransferase activity |
IDA | 11384981 | | GO:0008917 | lipopolysaccharide N-acetylglucosaminyltransferase activity |
IEA | -- | | GO:0016757 | transferase activity, transferring glycosyl groups |
IEA | -- | About this table | Pathways & Interactions for B3GNT5
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
| 2 Sigma-Aldrich "Your Favorite Gene" Pathways for B3GNT5 (Your Favorite Gene powered by Ingenuity) 
UniProtKB/Swiss-Prot: B3GN5_HUMAN, Q9BYG0Pathway: Protein modification; protein glycosylation 5/10 Interacting proteins for B3GNT5 (ENSP000003161733) via UniProtKB, MINT, and/or STRING (see all 10
)About this table
4 Gene Ontology (GO) biological process terms (links to tree view): About this table
|
Drugs & Compounds for B3GNT5(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for B3GNT5
|
Transcripts for B3GNT5(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_032047
Sigma-Aldrich siRNA for B3GNT5  Sigma-Aldrich shRNA for B3GNT5  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_032047 REFSEQ mRNAs for B3GNT5 gene: NM_032047.4
Applied Biosystems TaqMan ® Gene Expression Assays: NM_032047               OriGene GFP tagged cDNA clone in CMV expression vector: NM_032047                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_032047                                  untagged cDNA clones in CMV expression vector (see all 2): NM_032047  Additional cDNA sequence: AB045278.1 AB209517.1 AF368169.1 AJ304505.1 AK074235.1 AK074424.1 AK091474.1 BC013229.1 BC040935.1 11 DOTS entries: DT.91907482 DT.311018 DT.92028182 DT.100785405 DT.100785406 DT.120868479 DT.120868381 DT.120868418 DT.40111816 DT.95162130 DT.95162132 24/123 AceView cDNA sequences (see all 123
):AW516070 AJ304505 BF223815 BM142526 AK126337 N57022 BU684277 BF940440 BC028058 BQ722475 AI825936 BM142352 NM_032047 AX747008 AB045278 AI467964 BX499010 BF109408 AW026723 AW452443 AK091474 BG236076 D81847 AK074235
highest scoring ESTs for B3GNT5:AI825936 AA043551 AA369063 AA670337 AB045278 AF368169 AI039673 AI391576 AI445181 AI561139 Unigene Cluster for B3GNT5: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 Hs.718506 [show with all ESTs]Unigene Representative Sequence: NM_032047
GeneLoc Exon Structure
5 Alternative Splicing Database (ASD) splice patterns (SP) for B3GNT5
| ExUns: | 1 | ^ | 2 | ^ | 3a | · | 3b | · | 3c | · | 3d | ^ | 4 | ^ | 5a | · | 5b | · | 5c | · | 5d | |
| SP1: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP2: | |   | |   | |   | |   | |   | - |   | |   | |   | |   | |   | |   | |
| SP3: | |   | |   | |   | |   | - |   | - |   | |   | |   | |   | |   | |   | |
| SP4: | |   | - |   | - |   | - |   | - |   | - |   | - |   | |   | |   | |   | |   | |
| SP5: | |   | |   | - |   | - |   | - |   | - |   | - |   | |   | |   | |   | |   |
About this scheme
ECgene alternative splicing isoforms for B3GNT5
1 Ensembl transcript including schematic representation: ENST00000326505
|
Expression for B3GNT5
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| B3GNT5 expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for B3GNT5
1 / 2 / 3 5 probe-sets matching B3GNT5 gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: TTCCTTGTAA
SOURCE GeneReport for Unigene cluster: Hs.718506 UniProtKB/Swiss-Prot: B3GN5_HUMAN, Q9BYG0Tissue specificity: Widely expressed. Highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum. Weakly expressed in brain, liver, spleen, lymph node and thymus |
Orthologs for B3GNT5
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for B3GNT5 gene from 5/6 species (see all 6
)
About this table Species with no ortholog for B3GNT5
ENSEMBL Gene Tree for B3GNT5 | Paralogs for B3GNT5(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Paralogs for B3GNT5 gene
- B3GALT42 ENSG000002146542
|
SNPs/Variants for B3GNT5(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
| -- |
Disorders & Mutations for B3GNT5
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
| -- |
Medical News for B3GNT5(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for B3GNT5 (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 8 PubMed articles for B3GNT5 gene:- Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta 1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids. (PubMed id 11283017)2, 3, 4 Togayachi A....Narimatsu H. (2001)
- Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis. (PubMed id 11384981)3, 4 Henion T.R.... Hennet T. (2001)
- Helicobacter pylori induces beta3GnT5 in human gastric cell lines, modulating expression of the SabA ligand sialyl-Lewis x. (PubMed id 18483624)3 Marcos N.T....Reis C.A. (2008)
- Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions. (PubMed id 15342556)3 Suzuki Y.... Sugano S. (2004)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)4 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)3 Ota T....Sugano S. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)3 Strausberg R.L....Marra M.A. (2002)
- Normalization and subtraction: two approaches to facilitate gene discovery. (PubMed id 8889548)3 Bonaldo M.F....Soares M.B. (1996)
|
Search for B3GNT5
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing B3GNT5
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing B3GNT5
(According to HUGE)
About This Section
| -- |
Specialized Databases showing B3GNT5(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| Name | Description |
| GGDB | http://riodb.ibase.aist.go.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=B3GNT5 | | Functional Glycomics Gateway - GTase | http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_538 |
|
| | | About This Section
| --
| Services for B3GNT5(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for B3GNT5:

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Incremental update: 13 Oct 2009
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