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B3GNT3 Gene

protein-coding   GIFtS: 59

GC19P017766
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: TMEM3)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
B3GAL-T8 2
B3GALT8 3
B3GN-T3 1, 2
B3GNT-3 1, 2
BGnT-3 3
Beta-3-Gx-T8 3
Beta3Gal-T8 3
Beta3Gn-T3 3
Core1-beta3GlcNAcT 3
EC 2.4.1.- 3
HP10328 1, 2
TMEM3 2, 3, 5
b3Gal-T8 3
beta3Gn-T3 1, 2
Descriptions
Beta-1,3-GalTase 8 3
Beta-1,3-galactosyltransferase 8 3
Core 1 extending beta-1,3-N-acetylglucosaminyltransferase 3
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 8 3
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase
3 2
UDP-galactose:beta-N-acetylglucosamine
beta-1,3-galactosyltransferase 8 3
beta-1,3-N-acetylglucosaminyltransferase bGnT-3 2
putative type II membrane protein 2
transmembrane protein 3 2
External Ids
HGNC: 135281
Entrez Gene: 103312
UniProtKB: Q9Y2A93
Ensembl: ENSG000001799137
Search outside databases for aliases for B3GNT3 gene

Previous GC identifers: GC19P018399 GC19P018298 GC19P017751

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for B3GNT3:
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a
type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the
substrates of lacto-N-tetraose and lacto-N-neotetraose, and is involved in the biosynthesis of
poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl
Lewis a. It plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and
lymphocyte trafficking. [provided by RefSeq]

UniProtKB/Swiss-Prot: B3GN3_HUMAN, Q9Y2A9
Function: Has a beta-1,3-N-acetylglucosaminyltransferase activity for type 2 oligosaccharides

Gene Wiki entry for B3GNT3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the B3GNT3 gene  

Entrez Gene cytogenetic band: 19p13.1   Ensembl cytogenetic band:  19p13.11   HGNC cytogenetic band: 19p13.1

B3GNT3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P017766:     (about GC identifiers)

Start:
17,766,919 bp from pter
End:
17,785,385 bp from pter
Size:
18,467 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000019.8  NT_011295.10  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: B3GN3_HUMAN, Q9Y2A9 (See protein sequence)
Recommended Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3  
Size: 372 amino acids; 42515 Da
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein (Potential)
Sequence caution: Sequence=CAC82374.1; Type=Frameshift; Positions=194, 196, 202, 204, 209, 215;
Secondary accessions: B2RAS4 Q6NWU9 Q6NXU9 Q8WWR6 Q9C0J2

REFSEQ proteins: NP_055071.2  

ENSEMBL proteins: 
ENSP00000321874 


Human Recombinant Proteins 
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Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (B3GNT3)
Human Recombinant Proteins from Abnova (B3GNT3)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000139 Golgi membrane IEA--
GO:0005794 Golgi apparatus IEA--
GO:0005887 integral to plasma membrane TAS10072769
GO:0016020 membrane IEA--
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Antibodies for B3GNT3: 
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Sigma-Aldrich Antibodies for B3GNT3
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Antibodies from Abcam (B3GNT3), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (B3GNT3)
Novus Biologicals Antibodies for B3GNT3

Assays for B3GNT3: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR002659 Glyco_trans_31


   GeneDecks  B3GNT3 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9Y2A9

ProtoNet protein and cluster: Q9Y2A9

1 Blocks protein family: IPB002659 Glycosyl transferase

UniProtKB/Swiss-Prot: B3GN3_HUMAN, Q9Y2A9
Similarity: Belongs to the glycosyltransferase 31 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (B3GNT3)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (B3GNT3)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014256

              Applied Biosystems Silencer® siRNAs for B3GNT3

              Sigma-Aldrich siRNA for B3GNT3  
                     Sigma-Aldrich shRNA for B3GNT3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for B3GNT3
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_014256
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_014256
                                 untagged cDNA clone in CMV expression vector: NM_014256 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_014256

UniProtKB/Swiss-Prot: B3GN3_HUMAN, Q9Y2A9
Function: Has a beta-1,3-N-acetylglucosaminyltransferase activity for type 2 oligosaccharides
Enzyme Number (IUBMB): EC 2.4.1.- 

2 MGI mutant phenotypes (inferred from 1 allele(MGI details for B3gnt3):

behavior/neurologicaltouch/vibrissae

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0008378 galactosyltransferase activity IEA--
GO:0016757 transferase activity, transferring glycosyl groups IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


1 Kegg Pathway  (Kegg details for B3GNT3):
 hsa00601 Glycosphingolipid biosynthesis - lacto and neolacto series

   GeneDecks  B3GNT3 for the pathways selected above  
About GeneDecksing
UniProtKB/Swiss-Prot: B3GN3_HUMAN, Q9Y2A9
Pathway: Protein modification; protein glycosylation


5/15 Interacting proteins for B3GNT3 (ENSP000003218743) via UniProtKB, MINT, and/or STRING (see all 15 )

InteractantInteraction Details
GeneCardExternal ID(s)
B3GNT1ENSP000003055953STRING (score=.911)
B4GALT1ENSP000003690553STRING (score=.899)
B4GALT2ENSP000003613993STRING (score=.899)
B4GALT3ENSP000003569773STRING (score=.899)
B4GALT4ENSP000003521443STRING (score=.899)
About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006486 protein amino acid glycosylation IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for B3GNT3

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (B3GNT3)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (B3GNT3)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014256

              Sigma-Aldrich siRNA for B3GNT3  
                     Sigma-Aldrich shRNA for B3GNT3  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_014256  

REFSEQ mRNAs for B3GNT3 gene: 

NM_014256.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_014256  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_014256
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_014256
                                 untagged cDNA clone in CMV expression vector: NM_014256 

Additional cDNA sequence: 

BC066876.1 BC067423.1 

10 DOTS entries:

DT.95259878  DT.100786910  DT.91702134  DT.411543  DT.121431940  DT.100786905  DT.100786906  DT.121432096 
DT.121432184  DT.121432183 

24/131 AceView cDNA sequences (see all 131 ):

CR611299 AI738415 BM718996 BX483385 AW848963 BM837166 NM_014256 AB049585 
CR614648 AI337293 BM682980 BM751524 AF293973 AB015630 AJ130847 R77875 
BF109635 BM821698 AK075466 AI669036 AK075434 AI684023 BX359759 CD559581 

highest scoring ESTs for B3GNT3:

AB015630 AA131918 AA172052 AA367608 AA552420 AA552613 AA569131 AB049585 AF293973 AI023867 

Unigene Clusters for B3GNT3:

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
Hs.69009  [show with all ESTs], Hs.657825  [show with all ESTs]
Unigene Representative Sequences: NM_014256, BC066876


GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for B3GNT3

ExUns: 1 ^ 2a · 2b ^ 3a · 3b · 3c ^ 4a · 4b · 4c
SP1:        -     -     -                                 
SP2:                    -                                 
SP3:                                                      
SP4:                    -                                 

About this scheme

ECgene alternative splicing isoforms for B3GNT3

1 Ensembl transcript including schematic representation:
ENST00000318683  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

B3GNT3 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for B3GNT3

1 / 2 / 3

3 probe-sets matching B3GNT3 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

36832_at2, 3 U95-A 2 1.00 0.97 1.00 1.00 AB015630 1.00 1.00 1.00 1

204856_at2, 3 U133-A 1 1.00 1.00 -- -- NM_014256 0.60 1.00 0.82 1

204856_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene clusters: Hs.69009 Hs.657825

Expression variation in blood from EXPOLDB for B3GNT3

UniProtKB/Swiss-Prot: B3GN3_HUMAN, Q9Y2A9
Tissue specificity: Expressed in colon, jejunum, stomach, esophagus, placenta and trachea

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for B3GNT3 gene from 5/6 species (see all 6 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
B3GNT31   -- UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more 79.66(n)
73.12(a)
484831  XM_541947.1  XP_541947.1 
chimpanzee
(Pan troglodytes)
B3GNT31   -- UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more 99.28(n)
99.46(a)
455847  XM_001173648.1  XP_001173648.1 
rat
(Rattus norvegicus)
B3gnt31   -- UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more 70.74(n)
67.78(a)
290638  XM_224719.2  XP_224719.2 
mouse
(Mus musculus)
B3gnt31, 5 85
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more1, 5 70.46(n)1
68.06(a)1
722971  NM_028189.21  NP_082465.21 
 AK0086745  AK1441175  (see all 11)
zebrafish
(Danio rerio)
CH211-126G16.111   -- similar to beta-3-galactosyltransferase 58.47(n)
56.63(a)
561028  XM_684430.3  XP_689522.2 
About this table        Species with no ortholog for B3GNT3

ENSEMBL Gene Tree for B3GNT3
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for B3GNT3 gene
B3GNT72  B3GNT42  

Pseudogenes
2 related pseudogenes


(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/174 NCBI SNPs in B3GNT3 are shown (see all 174 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 53)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 19 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs7224291,2
A,C,F,H,O17785753(+) AGGAGC/TGGGAG 1 -- ng3112Minor allele frequency- N:0.00EA NA EU WA 1056
rs2655391,2
C,F,H,O17784160(+) AATATC/GCATCT 1 -- ut31 ese37Minor allele frequency- G:0.14MN EA EU WA 1852
rs142681,2
A,C,F,H17784795(+) CCCTGC/TAGCCC 1 -- ut31 ese38Minor allele frequency- T:0.34MN EA WA 1772
rs366861,2
A,C,F,H17783795(+) GCAACA/GCCTGT 1 R/H mis1 ese316Minor allele frequency- G:0.38MN EA EU WA NA 4116
rs2655411,2
C,F,H17765444(+) GGCCGA/GCAAAT 1 -- ng513Minor allele frequency- G:0.04EA WA 274
rs108541611,2
A,C,F17766619(+) GTGCCA/GGGAAC 1 -- ng514Minor allele frequency- G:0.24EA NA 202
rs176833951,2
F,H17784414(+) CCTGAT/CCTTAA 1 -- ut317Minor allele frequency- C:0.02NA EU EA WA 562
--
rs284036121,2
F17784556(+) GTGTCC/-TTTGA 1 -- ut312Minor allele frequency- -:0.48MN EA 1252
--
rs7224281,2
A17785483(+) GCAACC/TTCCGC 1 -- ng310--------
--
rs38332871,2
C17784555(-) CAAAG-/GACACT 1 -- ut310--------
About this table

HapMap Linkage Disequilibrium images for B3GNT3 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 605863

Human Gene Mutation Database: B3GNT3
Human Genome Epidemiology Navigator: B3GNT3 (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/16 PubMed articles for B3GNT3 gene (see all 16 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 10331 HGNC: 13528 AceView: B3GNT3 Ensembl:ENSG00000179913 euGenes: HUgn10331
ECgene: B3GNT3 H-InvDB: B3GNT3
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
GGDBhttp://riodb.ibase.aist.go.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=B3GNT3
Functional Glycomics Gateway - GTasehttp://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_536
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for B3GNT3:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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