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B3GNT2 Gene

protein-coding   GIFtS: 54

GC02P062276
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
(Previous name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: B3GNT1)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
B3GALT7 3
B3GN-T1 1, 2
B3GN-T2 1, 2
B3GNT 2
B3GNT-2 1, 2
B3GNT1 2, 3
BETA3GNT 1, 2
BGnT-1 3
Beta-3-Gx-T7 3
Beta3Gal-T7 3
Beta3Gn-T1 3
EC 2.4.1.- 3
b3Gal-T7 3
beta3gal-T5 2
Descriptions
Beta-1,3-GalTase 7 3
Beta-1,3-galactosyltransferase 7 3
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7 3
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase
1 1, 2
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase
2 2
UDP-galactose:beta-N-acetylglucosamine
beta-1,3-galactosyltransferase 7 3
beta-1,3-N-acetylglucosaminyltransferase bGnT-1 2
beta-1,3-N-acetylglucosaminyltransferase bGnT-2 2
External Ids
HGNC: 156291
Entrez Gene: 106782
UniProtKB: Q9NY973
Ensembl: ENSG000001703407
Search outside databases for aliases for B3GNT2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for B3GNT2:
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a
type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in
the biosynthesis of poly-N-acetyllactosamine chains. [provided by RefSeq]

UniProtKB/Swiss-Prot: B3GN2_HUMAN, Q9NY97
Function: Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains

Gene Wiki entry for B3GNT2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the B3GNT2 gene  

Entrez Gene cytogenetic band: 2p15   Ensembl cytogenetic band:  2p15   HGNC cytogenetic band: 2p15

B3GNT2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P062276:     (about GC identifiers)

Start:
62,276,766 bp from pter
End:
62,305,370 bp from pter
Size:
28,605 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_022184.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: B3GN2_HUMAN, Q9NY97 (See protein sequence)
Recommended Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2  
Size: 397 amino acids; 46022 Da
Cofactor: Manganese
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein (Potential)
Caution: Was indicated as B3Gal-T6 in submitted DNA entries
Secondary accessions: Q9NQQ9 Q9NQR0 Q9NUT9
Alternative splicing: 2 isoforms:  Q9NY97-1   Q9NY97-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006568.2  

    ENSEMBL proteins: 
    ENSP00000384692 ENSP00000305595 ENSP00000344143 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (B3GNT1)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000139 Golgi membrane IEA--
    GO:0005794 Golgi apparatus IEA--
    GO:0016020 membrane IEA--
    GO:0016021 integral to membrane NAS--
    About this table

    Antibodies for B3GNT2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for B3GNT2
    R&D Systems Antibodies for B3GNT2
    Antibodies from Abcam (B3GNT1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (B3GNT2)
    Novus Biologicals Antibodies for B3GNT2

    Assays for B3GNT2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR002659 Glyco_trans_31


       GeneDecks  B3GNT2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9NY97

    ProtoNet protein and cluster: Q9NY97

    1 Blocks protein family: IPB002659 Glycosyl transferase

    UniProtKB/Swiss-Prot: B3GN2_HUMAN, Q9NY97
    Similarity: Belongs to the glycosyltransferase 31 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (B3GNT2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (B3GNT2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_033252

                  Applied Biosystems Silencer® siRNAs for B3GNT2

                  Sigma-Aldrich siRNA for B3GNT2  
                         Sigma-Aldrich shRNA for B3GNT2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for B3GNT2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006577
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006577
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_006577 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006577

    UniProtKB/Swiss-Prot: B3GN2_HUMAN, Q9NY97
    Function: Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains
    Enzyme Number (IUBMB): EC 2.4.1.- 

    6 MGI mutant phenotypes (inferred from 3 alleles(MGI details for B3gnt2):

    behavior/neurologicalhematopoietic systemimmune systemnervous systemrespiratory system
    taste/olfaction

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008378 galactosyltransferase activity IEA--
    GO:0008499 UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity NAS--
    GO:0016757 transferase activity, transferring glycosyl groups IEA--
    GO:0030145 manganese ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    2 Sigma-Aldrich "Your Favorite Gene" Pathways for  B3GNT2  (Your Favorite Gene powered by Ingenuity) 
     Glycosphingolipid Biosynthesis - Neolactoseries
     Keratan Sulfate Biosynthesis

       GeneDecks  B3GNT2 for the pathways selected above  
    About GeneDecksing

    2 Kegg Pathways  (Kegg details for B3GNT2):
     hsa00533 Keratan sulfate biosynthesis
     hsa00601 Glycosphingolipid biosynthesis - lacto and neolacto series

       GeneDecks  B3GNT2 for the pathways selected above  
    About GeneDecksing
    UniProtKB/Swiss-Prot: B3GN2_HUMAN, Q9NY97
    Pathway: Protein modification; protein glycosylation


    5/16 Interacting proteins for B3GNT2 (ENSP000003055953) via UniProtKB, MINT, and/or STRING (see all 16 )

    InteractantInteraction Details
    GeneCardExternal ID(s)
    B3GNT3ENSP000003218743STRING (score=.911)
    B3GNT4ENSP000003196363STRING (score=.911)
    FUT1ENSP000003120213STRING (score=.909)
    FUT3ENSP000003056033STRING (score=.909)
    FUT4ENSP000003516023STRING (score=.909)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006486 protein amino acid glycosylation IEA--
    GO:0008150 biological_process ND--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for B3GNT2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (B3GNT2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (B3GNT2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_033252

                  Sigma-Aldrich siRNA for B3GNT2  
                         Sigma-Aldrich shRNA for B3GNT2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006577  

    REFSEQ mRNAs for B3GNT2 gene: 

    NM_006577.5   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006577  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006577
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006577
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_006577 

    Additional cDNA sequence: 

    AB049584.1 AF092051.2 AF288208.1 AF288209.1 AJ006077.1 AK002009.1 BC030579.2 BC047933.1 
    XM_001718549.1 

    9 DOTS entries:

    DT.213887  DT.100748568  DT.100023248  DT.95164130  DT.121021177  DT.100716809  DT.100773165  DT.121021201 
    DT.91996120 

    24/124 AceView cDNA sequences (see all 124 ):

    D87736 AA936769 BM557603 BQ101164 AA310578 BU753670 CK823150 AW136376 
    BX106672 AW613027 AA381830 BQ029038 AA835126 AI862049 BQ000660 AA377081 
    AA873030 AA381631 BG319463 BU620612 AI638560 CD367444 AI569147 BC047933 

    highest scoring ESTs for B3GNT2:

    AA310578 AA377081 AA449869 AA773646 AA873030 AA936769 AB049584 AF092051 AF288208 AF288209 

    Unigene Cluster for B3GNT2:

    UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
    Hs.173203  [show with all ESTs]
    Unigene Representative Sequence: BC047933


    GeneLoc Exon Structure

    3 Ensembl transcripts including schematic representations:
    ENST00000405767  ENST00000301998  ENST00000340715  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    B3GNT2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for B3GNT2

    1 / 2 / 3

    4 probe-sets matching B3GNT2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    222870_s_at2, 3 U133-B 1 1.00 1.00 -- -- AF288208 0.80 1.00 0.91 1

    219326_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_006577 0.60 1.00 0.82 1

    222870_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    219326_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: ATATTTTAAA

    SOURCE GeneReport for Unigene cluster: Hs.173203
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for B3GNT2 gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    B3GNT21   -- UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more 89.25(n)
    91.94(a)
    474611  XM_531841.2  XP_531841.1 
    cow
    (Bos taurus)
    B3GNT21   -- UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more 88.89(n)
    90.48(a)
    515585  XM_864409.1  XP_869502.1 
    rat
    (Rattus norvegicus)
    B3gnt21   -- UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more 84.47(n)
    87.91(a)
    305571  XM_223674.3  XP_223674.1 
    mouse
    (Mus musculus)
    B3gnt21, 5 11 (12.00 cM)5
    UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more1, 5 84.97(n)1
    87.41(a)1
    536251  NM_016888.41  NP_058584.31 
     AA1191325  AA2043635  (see all 21)
    chicken
    (Gallus gallus)
    B3GNT21   -- UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more 74.14(n)
    74.56(a)
    421286  NM_001008457.1  NP_001008457.1 
    About this table        Species with no ortholog for B3GNT2

    ENSEMBL Gene Tree for B3GNT2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for B3GNT2 gene
    B3GNT82  

    Pseudogenes
    1 related pseudogene


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/58 NCBI SNPs in B3GNT2 are shown (see all 58 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 45)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs11361511,2
    A,C,F,H62304247(+) TTTTAC/TGGATG 1 -- ut31 ese312Minor allele frequency- N:0.00EA NA EU WA 1052
    rs38116161,2
    C,F,H62304135(-) TAATAT/CAGAAA 1 -- ut318Minor allele frequency- C:0.28EA NA EU WA 2056
    rs133936081,2
    F,H62304926(+) TGTCAA/GTTTAA 1 -- ut314Minor allele frequency- G:0.21EU EA WA 420
    rs67592491,2
    F,H62304173(+) TACCCA/GTTTGA 1 -- ut314Minor allele frequency- G:0.18EU EA WA 420
    rs118978991,2
    C,H62304387(+) TATTCC/TTACTT 1 -- ut31 ese35Minor allele frequency- T:0.01EU EA WA NA 510
    rs101894681,2
    A,C,F,H62302359(+) AATGCG/ATCACA 1 -- int14Minor allele frequency- A:0.38EU EA WA 418
    rs46724821,2
    A,C,F,H62301903(+) aggggA/Gtatac 1 -- int112Minor allele frequency- N:0.01EU EA WA NA 1052
    rs116867711,2
    A,C,F,H62293898(+) gcaacT/Ggtagt 1 -- int18Minor allele frequency- G:0.19EU EA WA 832
    rs101825721,2
    A,C,F,H62300418(+) TATCAC/TGGAAG 1 -- int14Minor allele frequency- T:0.38EU EA WA 420
    rs75703051,2
    A,C,F,H62295942(+) AGGTAG/AACCAT 1 -- int19Minor allele frequency- A:0.33NA EU EA WA 736
    About this table

    HapMap Linkage Disequilibrium images for B3GNT2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 605581

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/16 PubMed articles for B3GNT2 gene (see all 16 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 10678 HGNC: 15629 AceView: B3GNT1 Ensembl:ENSG00000170340 euGenes: HUgn10678
    ECgene: B3GNT2 H-InvDB: B3GNT2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    GGDBhttp://riodb.ibase.aist.go.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=B3GNT1
    Functional Glycomics Gateway - GTasehttp://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_434
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for B3GNT2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for B3GNT2 Antibodies for B3GNT2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for B3GNT2 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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