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B3GNT1 Gene

protein-coding   GIFtS: 46

GC11M065870
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
(Previous name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: B3GNT6)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
B3GN-T1 1, 2
B3GNT6 2, 3
BETA3GNTI 1, 2
EC 2.4.1.149 3
I-beta-1,3-N-acetylglucosaminyltransferase 3
i-beta-1,3-N-acetylglucosaminyltransferase 2
iGAT 1, 2
iGNT 1, 2
iGnT 1, 3
Descriptions
Poly-N-acetyllactosamine extension enzyme 3
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase
1 2, 3
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase
6 1, 2
beta-1,3-N-acetylglucosaminyltransferase bGnT-6 2
External Ids
HGNC: 156851
Entrez Gene: 110412
UniProtKB: O435053
Ensembl: ENSG000001703407
Search outside databases for aliases for B3GNT1 gene

Previous GC identifers: GC02P062551 GC02P062609 GC02P062380 GC02P062397 GC02P062334

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for B3GNT1:
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a
type II transmembrane protein. It is essential for the synthesis of poly-N-acetyllactosamine, a
determinant for the blood group i antigen. [provided by RefSeq]

UniProtKB/Swiss-Prot: B3GN1_HUMAN, O43505
Function: Can initiate the synthesis or the elongation of the linear
poly-N-acetyllactosaminoglycans

Gene Wiki entry for B3GNT1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the B3GNT1 gene  

Entrez Gene cytogenetic band: 11q13.1   Ensembl cytogenetic band:  2p15   HGNC cytogenetic band: 11q13.2

B3GNT1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M065870:     (about GC identifiers)

Start:
65,869,419 bp from pter
End:
65,871,737 bp from pter
Size:
2,319 bases
Orientation:
minus strand

1 alternative location:
Chr2+ 62,276,766-62,305,368     
RefSeq DNA sequence:
NC_000011.8  NT_033903.7  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: B3GN1_HUMAN, O43505 (See protein sequence)
Recommended Name: N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase  
Size: 415 amino acids; 47119 Da
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein
Secondary accessions: Q4TTN0

REFSEQ proteins: NP_006867.1  

ENSEMBL proteins: 
ENSP00000384692 ENSP00000305595 ENSP00000344143 


Human Recombinant Proteins 
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Browse recombinant and purified proteins available from Enzo Life Sciences
Browse Abcam for Recombinant Proteins
Human Recombinant Proteins from Abnova (B3GNT6)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

5 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000139 Golgi membrane IEA--
GO:0005794 Golgi apparatus IEA--
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
GO:0030173 integral to Golgi membrane NAS--
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Antibodies for B3GNT1: 
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Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for B3GNT1
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Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
Monoclonal and Polyclonal Antibodies from Abnova (B3GNT6)
Novus Biologicals Antibody for B3GNT1

Assays for B3GNT1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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ProtoNet protein and cluster: O43505

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (B3GNT1)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006876

              Applied Biosystems Silencer® siRNAs for B3GNT1

              Sigma-Aldrich siRNA for B3GNT1  
                     Sigma-Aldrich shRNA for B3GNT1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for B3GNT1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_006876
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_006876
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_006876 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_006876

UniProtKB/Swiss-Prot: B3GN1_HUMAN, O43505
Function: Can initiate the synthesis or the elongation of the linear
poly-N-acetyllactosaminoglycans
Catalytic activity: UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R
= UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R
Enzyme Number (IUBMB): EC 2.4.1.149 

1 MGI mutant phenotype (inferred from 1 allele(MGI details for B3gnt1):

immune system

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0008532 N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity NAS--
GO:0016757 transferase activity, transferring glycosyl groups IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


2 Sigma-Aldrich "Your Favorite Gene" Pathways for  B3GNT1  (Your Favorite Gene powered by Ingenuity) 
 Keratan Sulfate Biosynthesis
 Glycosphingolipid Biosynthesis - Neolactoseries

   GeneDecks  B3GNT1 for the pathways selected above  
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2 Kegg Pathways  (Kegg details for B3GNT1):
 hsa00533 Keratan sulfate biosynthesis
 hsa00601 Glycosphingolipid biosynthesis - lacto and neolacto series

   GeneDecks  B3GNT1 for the pathways selected above  
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UniProtKB/Swiss-Prot: B3GN1_HUMAN, O43505
Pathway: Protein modification; protein glycosylation


5/16 Interacting proteins for B3GNT1 (ENSP000003055953) via UniProtKB, MINT, and/or STRING (see all 16 )

InteractantInteraction Details
GeneCardExternal ID(s)
B3GNT3ENSP000003218743STRING (score=.911)
B3GNT4ENSP000003196363STRING (score=.911)
FUT1ENSP000003120213STRING (score=.909)
FUT3ENSP000003056033STRING (score=.909)
FUT4ENSP000003516023STRING (score=.909)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0030311 poly-N-acetyllactosamine biosynthetic process NAS--
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for B3GNT1
1 Novoseek chemical compound relationship for B3GNT1 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
n-acetyllactosamine 91.16 10 10358011 (2), 10747980 (2), 9405606 (2), 8262950 (1) (see all 5)
About this table


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (B3GNT1)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006876

              Sigma-Aldrich siRNA for B3GNT1  
                     Sigma-Aldrich shRNA for B3GNT1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_006876  

REFSEQ mRNAs for B3GNT1 gene: 

NM_006876.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_006876  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_006876
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_006876
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_006876 

Additional cDNA sequence: 

AF029893.1 AK075354.1 AK098389.1 AK098779.1 AK225182.1 AK293715.1 AK294604.1 BC021965.2 
CR591559.1 CR602074.1 CR617432.1 CR623114.1 

7 DOTS entries:

DT.100796520  DT.454471  DT.95155593  DT.120734094  DT.120734106  DT.120734111  DT.97844419 

24/289 AceView cDNA sequences (see all 289 ):

CB128751 BM704157 CK822463 NM_006876 BU730048 AI300634 AK098779 BQ581439 
BF475788 BQ072231 CR591559 AW264293 BM926886 AF029893 BF195700 AI186969 
BC021965 T08639 CB265649 AK075354 AI695845 BQ881961 BU684788 BU848688 

highest scoring ESTs for B3GNT1:

AF029893 AA325816 AA383563 AA464806 AA477524 AA679070 AA719295 AI085597 AI186969 AI288195 

Unigene Cluster for B3GNT1:

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
Hs.8526  [show with all ESTs]
Unigene Representative Sequence: NM_006876


GeneLoc Exon Structure

3 Ensembl transcripts including schematic representations:
ENST00000405767  ENST00000301998  ENST00000340715  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

B3GNT1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for B3GNT1

1 / 2 / 3

3 probe-sets matching B3GNT1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
33140_at2, 3 U95-A 2 1.00 0.50 1.00 1.00 AF029893 1.00 1.00 1.00 1

203188_at2, 3 U133-A 2 1.00 0.50 -- -- NM_006876 0.60 1.00 0.82 1

203188_at2 U133Plus2 2 1.00 0.50 -- -- -- -- -- -- --
GeneDecks  B3GNT1 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: GGTGACAGCC

SOURCE GeneReport for Unigene cluster: Hs.8526

UniProtKB/Swiss-Prot: B3GN1_HUMAN, O43505
Tissue specificity: In the adult, highly expressed in heart, brain, skeletal muscle and kidney and
to a lesser extent in placenta, pancreas, spleen, prostate, testis, ovary, small intestine and
colon. Very weak expression in lung, liver, thymus and peripheral blood leukocytes. In fetal
highly expressed in brain and kidney and to a lesser extent in lung and liver

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for B3GNT1 gene from 5/9 species (see all 9 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
B3GNT11   -- UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more 92.53(n)
98.07(a)
476013  XM_533222.2  XP_533222.2 
chimpanzee
(Pan troglodytes)
B3GNT11   -- UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more 99.84(n)
100(a)
451344  XM_508571.2  XP_508571.2 
cow
(Bos taurus)
B3GNT21   -- UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more 92.61(n)
97.35(a)
618055  NM_001034808.1  NP_001029980.1 
rat
(Rattus norvegicus)
B3gnt11   -- UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more 88.83(n)
96.87(a)
293667  XM_219693.4  XP_219693.3 
mouse
(Mus musculus)
B3gnt11, 5 195
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase more1, 5 88.67(n)1
96.63(a)1
1089021  NM_175383.21  NP_780592.11 
 AI4294235  AI8512395  (see all 13)
About this table        Species with no ortholog for B3GNT1

ENSEMBL Gene Tree for B3GNT1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for B3GNT1 gene
B3GNT82  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

  --
(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 605517

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/17 PubMed articles for B3GNT1 gene (see all 17 ):
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 11041 HGNC: 15685 AceView: B3GNT6 Ensembl:ENSG00000170340 euGenes: HUgn11041
ECgene: B3GNT1 H-InvDB: B3GNT1
(According to HUGE)
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  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
GGDBhttp://riodb.ibase.aist.go.jp/rcmg/ggdb/
SeattleSNPshttp://pga.gs.washington.edu/data/b3gnt6/
Functional Glycomics Gateway - GTasehttp://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_547
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for B3GNT1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Antibodies for B3GNT1

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