B3GNT1 Gene
protein-coding GIFtS: 46
GC11M065870
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UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1 (Previous name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 ) Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database (Previous symbol: B3GNT6)
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Aliases & Descriptions for B3GNT1
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
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| Aliases |
|---|
| B3GN-T1 1, 2 | | B3GNT6 2, 3 | | BETA3GNTI 1, 2 | | EC 2.4.1.149 3 | | I-beta-1,3-N-acetylglucosaminyltransferase 3 | | i-beta-1,3-N-acetylglucosaminyltransferase 2 | | iGAT 1, 2 | | iGNT 1, 2 | | iGnT 1, 3 |
| | | Descriptions |
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| Poly-N-acetyllactosamine extension enzyme 3 | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1 2, 3 | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 1, 2 | | beta-1,3-N-acetylglucosaminyltransferase bGnT-6 2 |
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| | Search outside databases for aliases for B3GNT1 genePrevious GC identifers: GC02P062551 GC02P062609 GC02P062380 GC02P062397 GC02P062334 |
Summaries for B3GNT1(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for B3GNT1: This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It is essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen. [provided by RefSeq] UniProtKB/Swiss-Prot: B3GN1_HUMAN, O43505Function: Can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycansGene Wiki entry for B3GNT1 |
Genomic Location for B3GNT1
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the B3GNT1 gene 
Entrez Gene cytogenetic band: 11q13.1 Ensembl cytogenetic band: 2p15 HGNC cytogenetic band: 11q13.2B3GNT1 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 11 GeneLoc Exon Structure GeneLoc location for GC11M065870:
(about GC identifiers)
Start:
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65,869,419 bp from pter |
End:
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65,871,737 bp from pter |
Size:
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2,319 bases |
Orientation:
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minus strand |
1 alternative location:
| Chr2+ 62,276,766-62,305,368 |
RefSeq DNA sequence:- NC_000011.8 NT_033903.7
| Proteins for B3GNT1
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: B3GN1_HUMAN, O43505 (See
protein sequence)Recommended Name: N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase Size: 415 amino acids; 47119 Da
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein
Secondary accessions: Q4TTN0REFSEQ proteins: NP_006867.1
ENSEMBL proteins: ENSP00000384692 ENSP00000305595 ENSP00000344143
Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 
5 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for B3GNT1: Assays for B3GNT1: | Protein
Domains/ Families for B3GNT1(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| ProtoNet protein and cluster: O43505 | Gene Function for B3GNT1
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006876
Applied Biosystems Silencer® siRNAs for B3GNT1
Sigma-Aldrich siRNA for B3GNT1  Sigma-Aldrich shRNA for B3GNT1  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_006876                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_006876                                  untagged cDNA clones in CMV expression vector (see all 2): NM_006876 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_006876
UniProtKB/Swiss-Prot: B3GN1_HUMAN, O43505Function: Can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycansCatalytic activity: UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-REnzyme Number (IUBMB): EC 2.4.1.149 1 MGI mutant phenotype (inferred from 1 allele ) (MGI details for B3gnt1):
2 Gene Ontology (GO) molecular function terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0008532 | N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity |
NAS | -- | | GO:0016757 | transferase activity, transferring glycosyl groups |
IEA | -- | About this table | Pathways & Interactions for B3GNT1
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
| 2 Sigma-Aldrich "Your Favorite Gene" Pathways for B3GNT1 (Your Favorite Gene powered by Ingenuity) 
UniProtKB/Swiss-Prot: B3GN1_HUMAN, O43505Pathway: Protein modification; protein glycosylation 5/16 Interacting proteins for B3GNT1 (ENSP000003055953) via UniProtKB, MINT, and/or STRING (see all 16
)About this table
1 Gene Ontology (GO) biological process term (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0030311 | poly-N-acetyllactosamine biosynthetic process |
NAS | -- | About this table
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Drugs & Compounds for B3GNT1(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
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Browse Tocris compounds for B3GNT1 1 Novoseek chemical compound relationship for B3GNT1 gene
About this table
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Transcripts for B3GNT1(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006876
Sigma-Aldrich siRNA for B3GNT1  Sigma-Aldrich shRNA for B3GNT1  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_006876 REFSEQ mRNAs for B3GNT1 gene: NM_006876.2
Applied Biosystems TaqMan ® Gene Expression Assays: NM_006876               OriGene GFP tagged cDNA clone in CMV expression vector: NM_006876                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_006876                                  untagged cDNA clones in CMV expression vector (see all 2): NM_006876  Additional cDNA sequence: AF029893.1 AK075354.1 AK098389.1 AK098779.1 AK225182.1 AK293715.1 AK294604.1 BC021965.2 CR591559.1 CR602074.1 CR617432.1 CR623114.1 7 DOTS entries: DT.100796520 DT.454471 DT.95155593 DT.120734094 DT.120734106 DT.120734111 DT.97844419 24/289 AceView cDNA sequences (see all 289
):CB128751 BM704157 CK822463 NM_006876 BU730048 AI300634 AK098779 BQ581439 BF475788 BQ072231 CR591559 AW264293 BM926886 AF029893 BF195700 AI186969 BC021965 T08639 CB265649 AK075354 AI695845 BQ881961 BU684788 BU848688
highest scoring ESTs for B3GNT1:AF029893 AA325816 AA383563 AA464806 AA477524 AA679070 AA719295 AI085597 AI186969 AI288195 Unigene Cluster for B3GNT1: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1 Hs.8526 [show with all ESTs]Unigene Representative Sequence: NM_006876
GeneLoc Exon Structure
3 Ensembl transcripts including schematic representations: ENST00000405767
ENST00000301998
ENST00000340715
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Expression for B3GNT1
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| B3GNT1 expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for B3GNT1
1 / 2 / 3 3 probe-sets matching B3GNT1 gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: GGTGACAGCC
SOURCE GeneReport for Unigene cluster: Hs.8526 UniProtKB/Swiss-Prot: B3GN1_HUMAN, O43505Tissue specificity: In the adult, highly expressed in heart, brain, skeletal muscle and kidney and to a lesser extent in placenta, pancreas, spleen, prostate, testis, ovary, small intestine and colon. Very weak expression in lung, liver, thymus and peripheral blood leukocytes. In fetal highly expressed in brain and kidney and to a lesser extent in lung and liver |
Orthologs for B3GNT1
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
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Orthologs for B3GNT1 gene from 5/9 species (see all 9
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About this table Species with no ortholog for B3GNT1
ENSEMBL Gene Tree for B3GNT1 | Paralogs for B3GNT1(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Paralogs for B3GNT1 gene
- B3GNT82
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SNPs/Variants for B3GNT1(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
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Disorders & Mutations for B3GNT1
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
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OMIM: 605517
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Medical News for B3GNT1(Possibly Related Articles in
Doctor's Guide)
About This Section
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Publications for B3GNT1 (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/17 PubMed articles for B3GNT1 gene (see all 17
):- Expression cloning of cDNA encoding a human beta-1,3-N- acetylglucosaminyltransferase that is essential for poly-N- acetyllactosamine synthesis. (PubMed id 9405606)1, 2, 3, 4 Sasaki K.... Fukuda M. (1997)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)3, 4 Gerhard D.S....Malek J. (2004)
- Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)3 Kimura K.... Sugano S. (2006)
- Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. (PubMed id 16303743)3 Otsuki T....Isogai T. (2005)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)3 Strausberg R.L....Marra M.A. (2002)
- Construction and characterization of a full length-enriched and a 5'- end-enriched cDNA library. (PubMed id 9373149)3 Suzuki Y.... Sugano S. (1997)
- Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. (PubMed id 8125298)3 Maruyama K. and Sugano S. (1994)
- Poly-N-acetyllactosamine extension in N-glycans and core 2- and core 4-branched O-glycans is differentially controlled by i-extension enzyme and different members of the beta 1,4-galactosyltransferase gene family. (PubMed id 10747980)1 Ujita M....Fukuda M. (2000)
- Poly-N-acetyllactosamine synthesis in branched N-glycans is controlled by complemental branch specificity of I-extension enzyme and beta1,4-galactosyltransferase I. (PubMed id 10358011)1 Ujita M....Fukuda M. (1999)
- Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches. (PubMed id 9915862)1 Yeh J.C....Fukuda M. (1999)
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Search for B3GNT1
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
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Genome Databases showing B3GNT1
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing B3GNT1
(According to HUGE)
About This Section
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Specialized Databases showing B3GNT1(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
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| Name | Description |
| GGDB | http://riodb.ibase.aist.go.jp/rcmg/ggdb/ | | SeattleSNPs | http://pga.gs.washington.edu/data/b3gnt6/ | | Functional Glycomics Gateway - GTase | http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_547 |
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| | | About This Section
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| Services for B3GNT1(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
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 Products for B3GNT1:

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GeneCards Homepage - Last full update: 2 Jul 2009
Incremental update: 13 Oct 2009
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