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B3GAT2 Gene

protein-coding   GIFtS: 53

GC06M071623
beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase S)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 2.4.1.135 3
GLCATS 2, 3
GlcAT-D 3
GlcAT-S 1, 3
Glucuronosyltransferase-S 3
KIAA1963 2, 3
MGC138535 2
OTTHUMP00000016710 2
OTTHUMP00000039277 2
UDP-glucuronosyltransferase-S 3
Descriptions
Beta-1,3-glucuronyltransferase 2 2, 3
UDP-glucuronyltransferase S 2
beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase
S) 2
galactosylgalactosylxylosylprotein
3-beta-glucuronosyltransferase 2 2
uridine diphosphate glucuronic acid:acceptor
glucuronosyltransferase 2
External Ids
HGNC: 9221
Entrez Gene: 1351522
UniProtKB: Q9NPZ53
Ensembl: ENSG000001123097
Search outside databases for aliases for B3GAT2 gene

Previous GC identifers: GC00U990665 GC06M071533 GC06M071562 GC06M071566

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for B3GAT2:
The product of this gene is a transmembrane protein belonging to the glucuronyltransferase family,
and catalyzes the transfer of a beta-1,3 linked glucuronic acid to a terminal galactose in
different glycoproteins or glycolipids containing a Gal-beta-1-4GlcNAc or Gal-beta-1-3GlcNAc
residue. The encoded protein is involved in the synthesis of the human natural killer-1 (HNK-1)
carbohydrate epitope, a sulfated trisaccharide implicated in cellular migration and adhesion in
the nervous system. [provided by RefSeq]

UniProtKB/Swiss-Prot: B3GA2_HUMAN, Q9NPZ5
Function: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and
glycoproteins (By similarity)

Gene Wiki entry for B3GAT2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the B3GAT2 gene  

Entrez Gene cytogenetic band: 6q13   Ensembl cytogenetic band:  6q13   HGNC cytogenetic band: 6q12

B3GAT2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06M071623:     (about GC identifiers)

Start:
71,623,637 bp from pter
End:
71,723,509 bp from pter
Size:
99,873 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000006.10  NT_007299.12  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: B3GA2_HUMAN, Q9NPZ5 (See protein sequence)
Recommended Name: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2  
Size: 323 amino acids; 36919 Da
Cofactor: Manganese
Subunit: Homodimer (Potential)
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein
PDB structures from and Proteopedia :
2D0J (3D)    
Secondary accessions: Q5JS09 Q8TF38 Q96NK4

REFSEQ proteins: NP_542780.1  

ENSEMBL proteins: 
ENSP00000230053 


Human Recombinant Proteins 
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Browse Abcam for Recombinant Proteins
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000139 Golgi membrane IEA--
GO:0005794 Golgi apparatus IEA--
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
About this table

Antibodies for B3GAT2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for B3GAT2
Browse R&D Systems for Antibodies
Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for B3GAT2

Assays for B3GAT2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR005027 Glyco_trans_43


   GeneDecks  B3GAT2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q9NPZ5

ProtoNet protein and cluster: Q9NPZ5

1 Blocks protein family: IPB005027 Glycosyl transferase

UniProtKB/Swiss-Prot: B3GA2_HUMAN, Q9NPZ5
Similarity: Belongs to the glycosyltransferase 43 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (B3GAT2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (B3GAT2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_080742

              Applied Biosystems Silencer® siRNAs for B3GAT2

              Sigma-Aldrich siRNA for B3GAT2  
                     Sigma-Aldrich shRNA for B3GAT2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_080742
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_080742
                                 untagged cDNA clones in CMV expression vector (see all 2): AK055248 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_080742

UniProtKB/Swiss-Prot: B3GA2_HUMAN, Q9NPZ5
Function: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and
glycoproteins (By similarity)
Catalytic activity: UDP-glucuronate +
3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP +
3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein
Enzyme Number (IUBMB): EC 2.4.1.135 

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0015018 galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity ISS--
GO:0016740 transferase activity IEA--
GO:0030145 manganese ion binding IEA--
GO:0046872 metal ion binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


1 Sigma-Aldrich "Your Favorite Gene" Pathway for  B3GAT2  (Your Favorite Gene powered by Ingenuity) 
 Chondroitin Sulfate Biosynthesis

   GeneDecks  B3GAT2 for the pathways selected above  
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2 Kegg Pathways  (Kegg details for B3GAT2):
 hsa00532 Chondroitin sulfate biosynthesis
 hsa00534 Heparan sulfate biosynthesis

   GeneDecks  B3GAT2 for the pathways selected above  
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UniProtKB/Swiss-Prot: B3GA2_HUMAN, Q9NPZ5
Pathway: Protein modification; protein glycosylation


5/8 Interacting proteins for B3GAT2 (ENSP000002300533) via UniProtKB, MINT, and/or STRING (see all 8 )

InteractantInteraction Details
GeneCardExternal ID(s)
CHST10ENSP000002642493STRING (score=.943)
CSGALNACT1ENSP000003308053STRING (score=.899)
CSGALNACT2ENSP000003635903STRING (score=.899)
B3GAT1ENSP000003078753STRING (score=.811)
B3GAT3ENSP000002654713STRING (score=.811)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016051 carbohydrate biosynthetic process ISS--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for B3GAT2
2 Novoseek chemical compound relationships for B3GAT2 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
paragloboside 91.30 1 15470230 (1)
glycolipid 61.34 1 15470230 (1)
About this table


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (B3GAT2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (B3GAT2)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_080742

              Sigma-Aldrich siRNA for B3GAT2  
                     Sigma-Aldrich shRNA for B3GAT2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_080742  

REFSEQ mRNAs for B3GAT2 gene: 

NM_080742.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_080742  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_080742
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_080742
                                 untagged cDNA clones in CMV expression vector (see all 2): AK055248 

Additional cDNA sequence: 

AB075843.1 AK290018.1 AY070019.1 BC113995.1 

10 DOTS entries:

DT.308112  DT.454075  DT.454074  DT.95310418  DT.100784972  DT.91971398  DT.100703908  DT.121383613 
DT.92407740  DT.95310431 

24/49 AceView cDNA sequences (see all 49 ):

BM693450 AW590323 AY070019 NM_080742 BM670746 AI138778 AB075843 F11751 
BG149793 BM698655 BF477827 AW292462 D25748 BE089137 CB164059 BE645738 
AI701137 BM717735 R21032 BI914787 AI954745 BM674929 R18896 AI702728 

highest scoring ESTs for B3GAT2:

AB075843 AY070019 BG699432 AA835648 AI138778 AI214308 AI954745 AK055248 AV724131 BI820132 

Unigene Cluster for B3GAT2:

Beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase S)
Hs.713609  [show with all ESTs]
Unigene Representative Sequence: AB075843


GeneLoc Exon Structure

2 Alternative Splicing Database (ASD) splice patterns (SP) for B3GAT2

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b
SP1:                                    
SP2:                                    

About this scheme

ECgene alternative splicing isoforms for B3GAT2

1 Ensembl transcript including schematic representation:
ENST00000230053  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

B3GAT2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for B3GAT2

1 / 2 / 3

5 probe-sets matching B3GAT2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
49149_at2, 3 U95-C 1 1.00 1.00 0.94 1.42 AI954745 0.60 1.00 0.82 1
38877_s_at2, 3 U95-A 1 1.00 1.00 0.94 0.62 W26748 0.20 1.00 0.72 1
60287_at2, 3 U95-C 1 0.94 1.00 0.99 1.09 R42143 0.40 1.00 0.76 1

239144_at2, 3 U133-B 1 1.00 1.00 -- -- AA835648 0.60 1.00 0.82 1

239144_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  B3GAT2 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TTCTATAAGC

SOURCE GeneReport for Unigene cluster: Hs.713609

Expression variation in blood from EXPOLDB for B3GAT2

UniProtKB/Swiss-Prot: B3GA2_HUMAN, Q9NPZ5
Tissue specificity: Expressed in the trachea, retina, spinal cord, hippocampus and other brain
regions, and, at lower levels, in testis and ovary

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for B3GAT2 gene from 5/9 species (see all 9 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
B3GAT21   -- beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase more 93.29(n)
96.28(a)
481875  NM_001013419.1  NP_001013437.1 
chimpanzee
(Pan troglodytes)
B3GAT21   -- beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase more 99.48(n)
100(a)
462813  NM_001037314.1  NP_001032391.1 
cow
(Bos taurus)
B3GAT21   -- beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase more 89.68(n)
93.5(a)
541595  NM_001014392.1  NP_001014414.1 
mouse
(Mus musculus)
B3gat21, 5 15
beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase more1, 5 88.54(n)1
90.71(a)1
2806451  NM_172124.21  NP_742122.21 
 AB0559025  AB0559035  (see all 11)
chicken
(Gallus gallus)
B3GAT21   -- beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase more 85.24(n)
92.09(a)
428638  NM_001031527.1  NP_001026698.1 
About this table        Species with no ortholog for B3GAT2

ENSEMBL Gene Tree for B3GAT2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for B3GAT2 gene
B3GAT12  B3GAT32  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/490 NCBI SNPs in B3GAT2 are shown (see all 490 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 180)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 6 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs109452751,2
A,C,F,H71724568(+) TTTTGC/TTGTCT 1 -- ng318Minor allele frequency- T:0.19EU EA WA 834
rs69000111,2
A,C,F,H71724271(+) AGAATC/GGCCTT 1 -- ng318Minor allele frequency- G:0.26EU EA WA 832
rs13203151,2
A,C,F,H71722867(-) AGGCCG/AGGGAG 1 -- ut51 ese35Minor allele frequency- A:0.27MN EU EA WA 604
rs94463131,2
A71722948(+) TTAGGA/GAGTGG 1 -- ut51 ese30--------
rs15744901,2
F71722707(+) CGGGCA/GCCCCC 1 G/G syn1 ese31Minor allele frequency- G:0.17MN 184
rs94463121,2
H71722572(+) GCTTTC/TTGCAC 1 Q/Q syn1 ese34Minor allele frequency- T:0.00EU EA WA 408
rs6870871,2
A,C,F,H,O71653461(-) TGAATG/CTTCTC 1 -- int114Minor allele frequency- C:0.27MN EU EA WA NA 1346
rs6857611,2
A,C,F,H,O71653774(-) GGGACG/ATGGGA 1 -- int18Minor allele frequency- A:0.47MN NA EU EA WA 746
--
rs5922431,2
A,C,F,H,O71652847(-) CTATCA/GGGATG 1 -- int15Minor allele frequency- G:0.48MN EU EA WA 509
rs4908871,2
A,C,F,H,O71657644(-) AGCCAC/TATAGC 1 -- int113Minor allele frequency- T:0.21MN EU EA WA NA 1238
About this table

HapMap Linkage Disequilibrium images for B3GAT2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 607497

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/15 PubMed articles for B3GAT2 gene (see all 15 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 135152 HGNC: 922 AceView: B3GAT2 Ensembl:ENSG00000112309 euGenes: HUgn135152
ECgene: B3GAT2 H-InvDB: B3GAT2
(According to HUGE)
About This Section

HUGE: KIAA1963
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
GGDBhttp://riodb.ibase.aist.go.jp/rcmg/ggdb/
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for B3GAT2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
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 Predesigned and custom siRNAs for B3GAT2 Antibodies for B3GAT2
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for B3GAT2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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