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B3GALT4 Gene

protein-coding   GIFtS: 57

GC06P033352
UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
BETA3GALT4 2
Beta3Gal-T4 3
EC 2.4.1.62 3
GAL-T2 3
GALT2 2
GALT4 2, 3
GalT4 1, 3
OTTHUMP00000029128 2
UDP-galactose:beta-N-acetyl-galactosamine-beta-1,3-galactosyltransferase 3
b3Gal-T4 3
beta3Gal-T4 1
Descriptions
Beta-1,3-GalTase 4 3
Ganglioside galactosyltransferase 3
UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase 4 2
UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase,
polypeptide 4 2
beta-3-galactosyltransferase 4 2
External Ids
HGNC: 9191
Entrez Gene: 87052
UniProtKB: O960243
Ensembl: ENSG000002042227
Search outside databases for aliases for B3GALT4 gene

Previous GC identifers: GC06U990168 GC06P033241 GC06P033237 GC06P033291 GC06P033252

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for B3GALT4:
This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family
encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different
donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars
(N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related
to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon.
The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT
proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas
beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes
during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups:
beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is oriented telomere
to centromere in close proximity to the ribosomal protein S18 gene. The functionality of the
encoded protein is limited to ganglioseries glycolipid biosynthesis. [provided by RefSeq]

UniProtKB/Swiss-Prot: B3GT4_HUMAN, O96024
Function: Involved in GM1/GD1B/GA1 ganglioside biosynthesis

Gene Wiki entry for B3GALT4

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the B3GALT4 gene  

Entrez Gene cytogenetic band: 6p21.3   Ensembl cytogenetic band:  6p21.32   HGNC cytogenetic band: 6p21.3

B3GALT4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06P033352:     (about GC identifiers)

Start:
33,352,895 bp from pter
End:
33,354,580 bp from pter
Size:
1,686 bases
Orientation:
plus strand

2 alternative locations:
Chr6+,c6_COX 33,313,589-33,315,282      Chr6+,c6_QBL 33,316,612-33,318,297     
RefSeq DNA sequence:
NC_000006.10  NT_007592.14  NT_113891.1  NT_113897.1  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: B3GT4_HUMAN, O96024 (See protein sequence)
Recommended Name: Beta-1,3-galactosyltransferase 4  
Size: 378 amino acids; 41537 Da
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_003773.1  

    ENSEMBL proteins: 
    ENSP00000363756 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
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    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (B3GALT4)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000139 Golgi membrane IEA--
    GO:0005794 Golgi apparatus IEA--
    GO:0016020 membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    Antibodies for B3GALT4: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Monoclonal and Polyclonal Antibodies from Abnova (B3GALT4)
    Novus Biologicals Antibody for B3GALT4

    Assays for B3GALT4: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR002659 Glyco_trans_31


       GeneDecks  B3GALT4 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O96024

    ProtoNet protein and cluster: O96024

    1 Blocks protein family: IPB002659 Glycosyl transferase

    UniProtKB/Swiss-Prot: B3GT4_HUMAN, O96024
    Similarity: Belongs to the glycosyltransferase 31 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (B3GALT4)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003782

                  Applied Biosystems Silencer® siRNAs for B3GALT4

                  Sigma-Aldrich siRNA for B3GALT4  
                         Sigma-Aldrich shRNA for B3GALT4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for B3GALT4
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003782
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003782
                                     untagged cDNA clone in CMV expression vector: NM_003782 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_003782

    UniProtKB/Swiss-Prot: B3GT4_HUMAN, O96024
    Function: Involved in GM1/GD1B/GA1 ganglioside biosynthesis
    Catalytic activity: UDP-galactose +
    N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-(1->4)-beta-D-glucosyl-N-acylsphingosine
    = UDP +
    D-galactosyl-(1->3)-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine
    Enzyme Number (IUBMB): EC 2.4.1.62 

    Genatlas biochemistry entry for B3GALT4:
    UDP galactose
    1 MGI phenotypic allele for B3galt4 (no phenotypes)

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008378 galactosyltransferase activity IEA--
    GO:0008499 UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity TAS9582303
    GO:0016757 transferase activity, transferring glycosyl groups IEA--
    GO:0047915 ganglioside galactosyltransferase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    1 Invitrogen iPath™ Online BioAtlas - Pathway for B3GALT4 (Maps provided by GeneGo):
     Sphingolipid metabolism

       GeneDecks  B3GALT4 for the pathways selected above  
    About GeneDecksing

    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  B3GALT4  (Your Favorite Gene powered by Ingenuity) 
     Glycosphingolipid Biosynthesis - Ganglioseries

       GeneDecks  B3GALT4 for the pathways selected above  
    About GeneDecksing

    1 Kegg Pathway  (Kegg details for B3GALT4):
     hsa00604 Glycosphingolipid biosynthesis - ganglio series

       GeneDecks  B3GALT4 for the pathways selected above  
    About GeneDecksing
    UniProtKB/Swiss-Prot: B3GT4_HUMAN, O96024
    Pathway: Protein modification; protein glycosylation


    5/27 Interacting proteins for B3GALT4 (O960242 ENSP000003637563) via UniProtKB, MINT, and/or STRING (see all 27 )

    InteractantInteraction Details
    GeneCardExternal ID(s)
    B3GALT2ENSP000003564043STRING (score=.915)
    B3GALT5ENSP000003699923STRING (score=.913)
    CD2ENSP000003584903STRING (score=.899)
    CD3DENSP000003006923STRING (score=.899)
    CD3EENSP000003545663STRING (score=.899)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006486 protein amino acid glycosylation TAS9582303
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for B3GALT4
    10/11 Novoseek chemical compound relationships for B3GALT4 gene (see all 11 )
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    laccer 95.09 9 11740154 (2), 15229397 (2), 12900410 (1), 8481397 (1) (see all 7)
    lactosylceramide 93.74 8 8808768 (1), 11740154 (1), 8481397 (1), 9343929 (1) (see all 7)
    glucosylceramide 85.89 10 8808768 (1), 9061365 (1), 11740154 (1), 8781979 (1) (see all 6)
    glycosphingolipid 83.82 3 11740154 (1), 8481397 (1)
    ganglioside 67.87 6 8536214 (1), 8005614 (1), 9582303 (1)
    udpglucose 66.55 4 9061365 (1), 8781979 (1), 1551920 (1)
    ceramide 59.93 2 11740154 (1), 1834652 (1)
    galactose 34.24 1 8005614 (1)
    gtp 30.67 1 9254052 (1)
    lipid 3.49 4 2129343 (2), 15229397 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (B3GALT4)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003782

                  Sigma-Aldrich siRNA for B3GALT4  
                         Sigma-Aldrich shRNA for B3GALT4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_003782  

    REFSEQ mRNAs for B3GALT4 gene: 

    NM_003782.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_003782  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003782
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003782
                                     untagged cDNA clone in CMV expression vector: NM_003782 

    Additional cDNA sequence: 

    AB026730.1 AF063595.1 AK075312.1 AK315647.1 BC032574.1 CR457093.1 CR619620.1 Y15061.1 

    3 DOTS entries:

    DT.92419828  DT.210266  DT.97788718 

    24/2732 AceView cDNA sequences (see all 2732 ):

    BM459560 BQ058583 BU859886 AA360159 BQ683069 BU740293 AA301569 BM852084 
    BQ677276 F17992 AA844830 AI249442 BU183658 BQ676420 CB409488 BG286422 
    AA349284 BU627674 BU148523 AU185683 BQ958546 BM806420 AA342475 BE222652 

    highest scoring ESTs for B3GALT4:

    AV749969 BI522492 BI764671 BI772577 BI824146 BM545419 BM547323 BM712103 BM906020 BM921735 

    Unigene Cluster for B3GALT4:

    UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4
    Hs.534375  [show with all ESTs]
    Unigene Representative Sequence: NM_003782


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000374626  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    B3GALT4 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for B3GALT4

    1 / 2 / 3

    3 probe-sets matching B3GALT4 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    35944_at2, 3 U95-A 2 1.00 0.50 1.00 1.00 AL031228 0.20 0.08 0.15 1

    210205_at2, 3 U133-A 2 1.00 0.55 -- -- AB026730 0.60 0.83 0.73 1

    210205_at2 U133Plus2 2 1.00 0.55 -- -- -- -- -- -- --
    GeneDecks  B3GALT4 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GCCCGATACG

    SOURCE GeneReport for Unigene cluster: Hs.534375

    Expression variation in blood from EXPOLDB for B3GALT4

    UniProtKB/Swiss-Prot: B3GT4_HUMAN, O96024
    Tissue specificity: Highly expressed in heart, skeletal muscle and pancreas and, to a lesser
    extent, in brain, placenta, kidney, liver and lung

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for B3GALT4 gene from 5/14 species (see all 14 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    B3GALT41   -- UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, more 83.33(n)
    83.33(a)
    481737  XM_538858.1  XP_538858.1 
    chimpanzee
    (Pan troglodytes)
    B3GALT41   -- UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, more 99.82(n)
    100(a)
    746800  XM_001170317.1  XP_001170317.1 
    rat
    (Rattus norvegicus)
    B3galt41   -- UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, more 79.47(n)
    82.29(a)
    171079  NM_133553.1  NP_598237.1 
    mouse
    (Mus musculus)
    B3galt41, 5 175
    UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, more1, 5 80.02(n)1
    81.74(a)1
    542181  NM_019420.11  NP_062293.11 
     AB0391645  AB0391655  (see all 22)
    zebrafish
    (Danio rerio)
    rps182   -- ribosomal protein S18 81.86(n) 192300  AY099517.1 
    About this table        Species with no ortholog for B3GALT4

    ENSEMBL Gene Tree for B3GALT4
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for B3GALT4 gene
    B3GNT52  ENSG000002146542  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/17 NCBI SNPs in B3GALT4 are shown (see all 17 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 47)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 6 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs726544871,2
    --33352788(+) GTGCCA/CCTCCA 1 -- ng510--------
    --
    rs626393201,2
    --33353331(+) TGGCCC/GTGCCC 1 L/V mis10--------
    --
    rs726544931,2
    --33354250(+) TTGCTC/GCTGGT 1 S/C mis10--------
    --
    rs726544891,2
    --33352841(+) CTTCCA/GGCGGG 1 -- ng510--------
    --
    rs455316371,2
    --33353314(+) CTCACC/TGGGGC 1 P/L mis10--------
    --
    rs726544861,2
    --33352784(+) TTTGGC/TGCCAC 1 -- ng510--------
    --
    rs349749781,2
    --33353039(+) CCCCA-/GGGTGC 1 -- ut510--------
    --
    rs726544851,2
    --33352760(+) GCTGGA/TCAGAC 1 -- ng510--------
    rs22312601,2
    --33353895(+) CACCGC/GGGGGC 1 G/R mis1 ese30--------
    --
    rs726544881,2
    --33352792(+) CACTCC/TACCCG 1 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for B3GALT4 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 603095

    2 Novoseek disease relationships for B3GALT4 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    atherosclerosis 37.65 1 15229397 (1)
    tumors 0.00 2 8481397 (2)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/33 PubMed articles for B3GALT4 gene (see all 33 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 8705 HGNC: 919 AceView: B3GALT4andRPS18andD6S2723E Ensembl:ENSG00000204222 euGenes: HUgn8705
    ECgene: B3GALT4 H-InvDB: B3GALT4
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    GGDBhttp://riodb.ibase.aist.go.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=B3GALT4
    Functional Glycomics Gateway - GTasehttp://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_431
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for B3GALT4:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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