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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

AZI2 Gene

protein-coding   GIFtS: 49
GCID: GC03M028340

5-azacytidine induced 2

  Search for AZI2
in our new
 Human Malady Compendium 
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for AZI2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
5-Azacytidine Induced 21 2     NF-Kappa-B-Activating Kinase-Associated Protein 12 3
NAP11 2 3 5     FLJ219391
AZ21 2 5     5-Azacytidine Induced Gene 22
TILP2 3 5     5-Azacytidine-Induced Protein 22
Nak-Associated Protein 12 3     

External Ids:    HGNC: 240021   Entrez Gene: 643432   Ensembl: ENSG000001635127   OMIM: 6099165   UniProtKB: Q9H6S13   

Export aliases for AZI2 gene to outside databases


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for AZI2:
AZI2, or NAP1, contributes to the activation of NFKB (see MIM 164011)-dependent gene expression by activating
IKK-related kinases, such as NAK (TBK1; MIM 604834) (Fujita et al., 2003 (PubMed 14560022)).(supplied by OMIM, Mar
2008)

UniProtKB/Swiss-Prot: AZI2_HUMAN, Q9H6S1
Function: Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the
phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced
activation of NF-kappa-B. Participates in IFN-beta promoter activation via TICAM1

Gene Wiki entry for AZI2


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000003.11  NC_018914.1  NT_022517.18  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the AZI2 gene promoter:
         Pax-5   NCX/Ncx   IRF-1   STAT5A   AREB6   SRY   POU2F1   POU2F1a   SEF-1 (1)   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidAZI2 promoter sequence
   Search SABiosciences Chromatin IP Primers for AZI2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat AZI2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3p24.1   Ensembl cytogenetic band:  3p24.1   HGNC cytogenetic band: 3p23

AZI2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
AZI2 gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03M028340:  view genomic region     (about GC identifiers)

Start:
28,356,494 bp from pter      End:
28,390,618 bp from pter
Size:
34,125 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: AZI2_HUMAN, Q9H6S1 (See protein sequence)
Recommended Name: 5-azacytidine-induced protein 2  
Size: 392 amino acids; 44935 Da
Subunit: Interacts with IKBKE, TBK1 and TICAM1
Subcellular location: Cytoplasm
Secondary accessions: A8K3M2 Q86W99 Q9BQF1
Alternative splicing: 2 isoforms:  Q9H6S1-1   Q9H6S1-3   

Explore the universe of human proteins at neXtProt for AZI2: NX_Q9H6S1

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9H6S1

  • AZI2 Protein expression data from MOPED and PaxDb:    About this image 
    AZI2 Protein Expression
    REFSEQ proteins (4 alternative transcripts): 
    NP_001127904.1  NP_001127905.1  NP_001258579.1  NP_071906.1  

    ENSEMBL proteins: 
     ENSP00000403133   ENSP00000419371   ENSP00000335609   ENSP00000391696   ENSP00000389577  
     ENSP00000412820   ENSP00000416380  

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    Uscn Proteins for AZI2

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IDA--
    GO:0005739mitochondrion IDA--
    GO:0005886plasma membrane IDA--

    AZI2 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    AZI2 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR024581 TBD

    Graphical View of Domain Structure for InterPro Entry Q9H6S1

    ProtoNet protein and cluster: Q9H6S1


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: AZI2_HUMAN, Q9H6S1
    Function: Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the
    phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced
    activation of NF-kappa-B. Participates in IFN-beta promoter activation via TICAM1

    Phenotypes:
         1 GenomeRNAi human phenotype for AZI2:

     Decreased viability with pacli 

    Animal Models:
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    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1TNF-alpha/NF-kB Signaling Pathway
    TNF-alpha/NF-kB Signaling Pathway1.00
    2RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways
    RIG-I-like receptor signaling pathway0.52

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    1 BioSystems Pathway for AZI2 
        TNF-alpha/NF-kB Signaling Pathway


    1         Kegg Pathway  (Kegg details for AZI2):
        RIG-I-like receptor signaling pathway


    AZI2 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for AZI2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 17)

    5/21 Interacting proteins for AZI2 (Q9H6S12, 3 ENSP000004193714) via UniProtKB, MINT, STRING, and/or I2D (see all 21)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TBK1Q9UHD22, 3, ENSP000003299674MINT-48042 MINT-49256 I2D: score=6 STRING: ENSP00000329967
    TICAM1Q8IUC63, ENSP000002482444I2D: score=2 STRING: ENSP00000248244
    N4BP1O751133, ENSP000002623844I2D: score=1 STRING: ENSP00000262384
    RNF31Q96EP03, ENSP000003151124I2D: score=1 STRING: ENSP00000315112
    TBKBP1A7MCY63, ENSP000003547774STRING: ENSP00000354777 I2D: score=1 
    About this table

    Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007249I-kappaB kinase/NF-kappaB cascade IEA--

    AZI2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    AZI2 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for AZI2
    1 Novoseek chemical compound relationship for AZI2 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    poly(i-c) 81.7 1 17142768 (1)

    Search CenterWatch for drugs/clinical trials and news about AZI2 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for AZI2 gene (4 alternative transcripts): 
    NM_001134432.1  NM_001134433.1  NM_001271650.1  NM_022461.4  

    Unigene Cluster for AZI2:

    5-azacytidine induced 2
    Hs.706676  [show with all ESTs]
    Unigene Representative Sequence: NM_001271650
    14 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000429369 ENST00000479665(uc003ceb.3 uc003cec.3) ENST00000295748
    ENST00000476174 ENST00000492044 ENST00000463512(uc003cee.4) ENST00000334100
    ENST00000488978 ENST00000470234 ENST00000420543(uc011axd.1 uc003ceg.2)
    ENST00000457172 ENST00000414162 ENST00000462936 ENST00000415852

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    hsa-miR-124* hsa-miR-323-3p hsa-miR-607 hsa-miR-448 hsa-miR-520d-5p hsa-miR-539 hsa-miR-3660 hsa-miR-885-5p
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    Inhib. RNA
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    Additional cDNA sequence: 

    AK025592.1 AK290637.1 AY151386.1 BX648471.1 

    21 DOTS entries:

    DT.101985273  DT.100657228  DT.92018610  DT.113830  DT.455028  DT.95179264  DT.95153234  DT.100820234 
    DT.92435283  DT.40206412  DT.102830469  DT.100820233  DT.75182203  DT.99959382  DT.120896259  DT.120896287 
    DT.120896316  DT.120896414  DT.95179271  DT.95244778  DT.95361598 

    24/304 AceView cDNA sequences (see all 304):

    AW338891 AV651892 AY151386 AA781213 AV651297 BU620775 W52104 AI913136 
    BE049514 C02531 BM763115 AI253219 AI811273 AI696547 AW341751 CB241278 
    AI336192 AI753698 AI097289 BF000175 CR618873 BX327318 BM981726 AV709569 

    GeneLoc Exon Structure

    5/10 Alternative Splicing Database (ASD) splice patterns (SP) for AZI2 (see all 10)    About this scheme

    ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b · 11c ^ 12a · 12b ^ 13
    SP1:                                -     -     -                                               -                       -     -     -                     
    SP2:                                -     -                                                     -                                                         
    SP3:                                                                                            -                                                         
    SP4:                                -     -     -                                   -     -     -                                                         
    SP5:                                -                                                                                                                     


    ECgene alternative splicing isoforms for AZI2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    AZI2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TGGCTGCATA
    AZI2 Expression
    About this image
    See AZI2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for AZI2

    SOURCE GeneReport for Unigene cluster: Hs.706676

    UniProtKB/Swiss-Prot: AZI2_HUMAN, Q9H6S1
    Tissue specificity: Widely expressed. Abundant expression seen in the pancreas and testis

        SABiosciences Expression via Pathway-Focused PCR Array including AZI2: 
              Antiviral Response in human mouse rat

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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for AZI2 gene from 4/10 species (see all 10)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Azi21 , 5 5-azacytidine induced gene 21, 5 84.86(n)1
    84.44(a)1
      9 (69.86 cM)5
    272151  NM_013727.31  NP_038755.11 
     1180404995 
    chicken
    (Gallus gallus)
    Aves AZI21 5-azacytidine induced 2 69.08(n)
    68.48(a)
      420660  XM_418759.3  XP_418759.3 
    lizard
    (Anolis carolinensis)
    Reptilia AZI26
    --
    57(a)
    1 ↔ 1
    1(195984607-196000518)
    zebrafish
    (Danio rerio)
    Actinopterygii azi21 5-azacytidine induced 2 51.57(n)
    42.67(a)
      406377  NM_214733.1  NP_999898.1 


    ENSEMBL Gene Tree for AZI2 (if available)
    TreeFam Gene Tree for AZI2 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for AZI2 gene
    1 SIMAP similar gene for AZI2 using alignment to 6 protein entries:     AZI2_HUMAN (see all proteins):
    TBKBP1

    AZI2 for paralogs           About GeneDecksing


    3 Pseudogenes.org Pseudogenes for AZI2
    PGOHUM00000246775 PGOHUM00000247104 PGOHUM00000232587


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/423 NCBI SNPs in AZI2 are shown (see all 423    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1883541491,2
    --28363426(+) GATTCC/TAAAGG 2 -- int10--------
    rs1916590981,2
    --28363546(+) TTTTAC/TCTAGA 2 -- int10--------
    rs173572061,2
    C,F,H--28363559(+) AGAGAT/GATCAA 2 -- int126Minor allele frequency- G:0.17NA NS EA WA 2728
    rs1427025891,2
    --28363948(+) CAAAGA/GAAAAT 2 -- ut310--------
    rs1510087781,2
    --28364010(+) GATATA/GATATA 2 -- ut310--------
    rs1408505731,2
    --28364033(+) CCACAC/TGAGAA 2 -- ut310--------
    rs1850273081,2
    --28364207(+) TGACAC/GCAATT 2 -- ut310--------
    rs1459244321,2
    --28364312(+) AATACA/CGTAGA 2 -- ut310--------
    rs1479100731,2
    --28364332(+) AGATAC/TTGAGT 2 -- ut310--------
    rs1416689721,2
    --28364346(+) AGATAC/TATGAA 2 -- ut310--------

    HapMap Linkage Disequilibrium report for AZI2 (28356494 - 28390618 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for AZI2: --

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    AZI2 for disorders           About MalaCards

    AZI2 for disorders           About GeneDecksing

    OMIM gene information: 609916    OMIM disorders: --


    Export disorders for AZI2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for AZI2 gene, integrated from 9 sources (see all 20):
    (articles sorted by number of sources associating them with AZI2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification of NAP1, a regulatory subunit of IkappaB kinase- related kinases that potentiates NF-kappaB signaling. (PubMed id 14560022)1, 2, 9 Fujita F.... Nakanishi M. (2003)
    2. NF-kappaB-activating kinase-associated protein 1 participates in TLR3/Toll-IL-1 homology domain-containing adapter molecule-1-mediated IFN regulatory factor 3 activation. (PubMed id 15611223)1, 2, 9 Sasai M....Seya T. (2005)
    3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    4. Isolation of the novel cDNA of a gene of which expression is induced by a demethylating stimulus. (PubMed id 10580148)1, 3 Miyagawa J....Tajima S. (1999)
    5. NAK-associated protein 1 participates in both the TLR3 and the cytoplasmic pathways in type I IFN induction. (PubMed id 17142768)1, 9 Sasai M....Seya T. (2006)
    6. Tripartite motif-containing protein 38 negatively regu lates TLR3/4- and RIG-I-mediated IFN-b production and antiviral response by targ eting NAP1. (PubMed id 22539786)1 Zhao W....Gao C. (2012)
    7. Mapping a dynamic innate immunity protein interaction network regulating type I interferon production. (PubMed id 21903422)1 Li S....Dorf M.E. (2011)
    8. Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
    9. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
    10. Large-scale proteomics analysis of the human kinome. (PubMed id 19369195)2 Oppermann F.S.... Daub H. (2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 64343 HGNC: 24002 AceView: AZI2 Ensembl:ENSG00000163512 euGenes: HUgn64343
    ECgene: AZI2 Kegg: 64343 H-InvDB: AZI2

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for AZI2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for AZI2 gene:
    Search GeneIP for patents involving AZI2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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    GeneCards Homepage - Last full update: 18 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 27 Apr 2013 , 14 May 2013

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