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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

AZI1 Gene

protein-coding   GIFtS: 52
GCID: GC17M079163

5-azacytidine induced 1

  Search for AZI1
in our new
 Human Malady Compendium 
Biological research products
for AZI1
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
5-Azacytidine Induced 11 2     Pre-Acrosome Localization Protein 12 3
KIAA11181 3 5     Cep1312 3
AZ11 2     ZA12 5
CEP1311 3     5-Azacytidine-Induced Protein 12
Centrosomal Protein Of 131 KDa2 3     Centrosomal Protein 131 KDa2

External Ids:    HGNC: 295111   Entrez Gene: 229942   Ensembl: ENSG000001415777   OMIM: 6134795   UniProtKB: Q9UPN43   

Export aliases for AZI1 gene to outside databases

Previous GC identifers: GC17M079725 GC17M079863 GC17M076777 GC17M074602


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: AZI1_HUMAN, Q9UPN4
Function: May play a role in spermatogenesis (By similarity)

Gene Wiki entry for AZI1


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000017.10  NC_018928.1  NT_010783.15  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the AZI1 gene promoter:
         ISGF-3   C/EBPbeta   Sp1   p53   LCR-F1   HEN1   Pax-3   ARP-1   Pax-4a   ATF   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidAZI1 promoter sequence
   Search SABiosciences Chromatin IP Primers for AZI1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat AZI1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17q25.3   Ensembl cytogenetic band:  17q25.3   HGNC cytogenetic band: 17q25.3

AZI1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
AZI1 gene location

GeneLoc information about chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17M079163:  view genomic region     (about GC identifiers)

Start:
79,163,393 bp from pter      End:
79,196,799 bp from pter
Size:
33,407 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: AZI1_HUMAN, Q9UPN4 (See protein sequence)
Recommended Name: 5-azacytidine-induced protein 1  
Size: 1083 amino acids; 122149 Da
Subcellular location: Cytoplasm, cytoskeleton, centrosome
Sequence caution: Sequence=BAA83070.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
Secondary accessions: A6NHI8 B2RN11 Q96F50
Alternative splicing: 3 isoforms:  Q9UPN4-1   Q9UPN4-2   Q9UPN4-3   (Contains a phosphoserine at position 489. Contains a phosphoserine at position 496)

Explore the universe of human proteins at neXtProt for AZI1: NX_Q9UPN4

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9UPN4

  • AZI1 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001009811.2  NP_055799.2  

    ENSEMBL proteins: 
     ENSP00000363914   ENSP00000269392   ENSP00000461374   ENSP00000460006   ENSP00000393583  
     ENSP00000459733   ENSP00000460098  
    Reactome Protein details: Q9UPN4
    Human Recombinant Protein Products: 
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    Uscn Proteins for AZI1

    Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005813colocalizes with centrosome IDA14654843
    GO:0005829cytosol TAS--
    GO:0015630microtubule cytoskeleton IDA--
    GO:0043231intracellular membrane-bounded organelle IDA--


    AZI1 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ProtoNet protein and cluster: Q9UPN4

    UniProtKB/Swiss-Prot: AZI1_HUMAN, Q9UPN4
    Similarity: Contains 1 IQ domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: AZI1_HUMAN, Q9UPN4
    Function: May play a role in spermatogenesis (By similarity)

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    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003674molecular_function ND--


    AZI1 for ontologies           About GeneDecksing


    2 GenomeRNAi human phenotypes for AZI1:
     Increased cell number in S and  Increased gamma-H2AX phosphory 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Recruitment of mitotic centrosome proteins and complexes
    Centrosome maturation1.00
    Mitotic G2-G2/M phases0.81
    Recruitment of mitotic centrosome proteins and complexes1.00
    Loss of proteins required for interphase microtubule organization from the centrosome0.78
    G2/M Transition0.83
    Loss of Nlp from mitotic centrosomes0.78
    2Cell Cycle
    Cell Cycle1.00
    Cell Cycle, Mitotic0.84

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    5/8        Reactome Pathways for AZI1 (see all 8)
        Centrosome maturation
    Cell Cycle
    Recruitment of mitotic centrosome proteins and complexes
    G2/M Transition
    Loss of proteins required for interphase microtubule organization from the centrosome



    AZI1 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for AZI1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/61 Interacting proteins for AZI1 (Q9UPN43 ENSP000003935834) via UniProtKB, MINT, STRING, and/or I2D (see all 61)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    OAZ1P543683, ENSP000003148134I2D: score=1 STRING: ENSP00000314813
    TP73O153503, ENSP000003675454I2D: score=1 STRING: ENSP00000367545
    YWHAEP622583, ENSP000002643354I2D: score=1 STRING: ENSP00000264335
    ODC1P119263, ENSP000002341114I2D: score=1 STRING: ENSP00000234111
    POT1Q9NUX53, ENSP000003502494I2D: score=1 STRING: ENSP00000350249
    About this table

    Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000086G2/M transition of mitotic cell cycle TAS--
    GO:0000278mitotic cell cycle TAS--
    GO:0007275multicellular organismal development IEA--
    GO:0007283spermatogenesis IEA--
    GO:0008150biological_process ND--


    AZI1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for AZI1
    Search CenterWatch for drugs/clinical trials and news about AZI1 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for AZI1 gene (2 alternative transcripts): 
    NM_001009811.2  NM_014984.2  

    Unigene Cluster for AZI1:

    5-azacytidine induced 1
    Hs.514578  [show with all ESTs]
    Unigene Representative Sequence: BC012130
    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000374782 ENST00000269392(uc002jzm.1 uc002jzn.1 uc002jzo.1 uc002jzp.1 uc010wum.1)
    ENST00000570817 ENST00000573053(uc002jzq.3) ENST00000450824 ENST00000575907
    ENST00000571292 ENST00000570482

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    Additional cDNA sequence: 

    AB029041.1 BC010413.2 BC011615.2 BC012130.1 BC040023.1 BC136578.1 BC136580.1 BC144263.1 

    6 DOTS entries:

    DT.70104946  DT.92424048  DT.100697986  DT.435887  DT.100802481  DT.92049878 

    24/65 AceView cDNA sequences (see all 65):

    BE384437 BQ637523 BE734213 BU165495 BC010413 BQ069739 BE902597 BM911459 
    BC040023 BG031900 BF591342 BC011615 AL135248 CO250199 BM927610 BQ643385 
    AB029041 BM009715 BF343215 BF974651 BX391524 BG109696 CF125276 AI817702 

    GeneLoc Exon Structure

    5/7 Alternative Splicing Database (ASD) splice patterns (SP) for AZI1 (see all 7)    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17 ^ 18a · 18b ^ 19a · 19b ^ 20 ^ 21 ^ 22a · 22b ^
    SP1:                                -                                                                                                                           
    SP2:                                                                                -                                                                           
    SP3:                                                                                                                                      -                     
    SP4:                                                                                                                                                            
    SP5:                                                                                                                                      -                     

    ExUns: 23a · 23b · 23c ^ 24a · 24b ^ 25 ^ 26 ^ 27
    SP1:  -                                             
    SP2:  -                                             
    SP3:  -                                             
    SP4:                                                
    SP5:                                                


    ECgene alternative splicing isoforms for AZI1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    AZI1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: AGGCTGCGGT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    AZI1 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    Stem Cell Differentiation: 3 LifeMap Cells 
    NameCategory
    ES-J1 (Embryonic Stem Cell)Early Embryo, Inner Cell Mass
    Long-time differentiated EBs (Derivation of embryo...)
    Short-term differentiated embryoid bodies (Derivation of embryo...)
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See AZI1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for AZI1

    SOURCE GeneReport for Unigene cluster: Hs.514578
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AZI1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for AZI1 gene from 6/21 species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Azi11 , 5 5-azacytidine induced gene 11, 5 78.97(n)1
    74.69(a)1
      11 (83.96 cM)5
    120091  NM_009734.31  NP_033864.31 
     1200644305 
    chicken
    (Gallus gallus)
    Aves AZI11 5-azacytidine induced 1 70.36(n)
    62.65(a)
      422074  XM_420077.3  XP_420077.3 
    lizard
    (Anolis carolinensis)
    Reptilia AZI16
    --
    61(a)
    1 ↔ 1
    2(99346166-99376090)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.253952 Xenopus laevis transcribed sequence with weak similarity more 73.44(n)    BJ058775.1 
    zebrafish
    (Danio rerio)
    Actinopterygii azi11 5-azacytidine induced gene 1 58.83(n)
    52.96(a)
      563066  XM_002664004.1  XP_002664050.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta dila6
    dilatory
    19(a)
    1 ↔ 1
    2R(5710582-5714988)


    ENSEMBL Gene Tree for AZI1 (if available)
    TreeFam Gene Tree for AZI1 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/914 NCBI SNPs in AZI1 are shown (see all 914    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 17 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs561101521,2
    C--74620033(+) AGCGGA/GCGCTA 1 -- int10--------
    rs785825561,2
    --79162898(+) GAGAGC/TTCCAG 2 -- int12Minor allele frequency- T:0.11CSA WA 120
    rs1822805661,2
    --79162918(+) CCTTGA/GCAGCG 2 -- int10--------
    rs1148764371,2
    C,F,--79163210(+) CTGGGG/AGCTGC 2 -- int11Minor allele frequency- A:0.02WA 118
    rs754664971,2
    C,--79163320(+) AGCCAA/GACCCA 2 -- int13Minor allele frequency- G:0.05NA EA 242
    rs1995238521,2
    --79163372(+) GAGAG-/AAGACAA 2 -- int10--------
    rs1472613221,2
    --79163406(+) AACAAC/TGGCCT 2 -- ut310--------
    rs1487547361,2
    --79163481(+) GGGGGC/TAGGTG 2 -- ut310--------
    rs1154348291,2
    C,F,--79163514(+) CCCACG/ATCCAG 2 -- ut311Minor allele frequency- A:0.02WA 118
    rs1421653891,2
    C,--79163539(+) CTTCCA/GTTCTT 2 -- ut310--------

    HapMap Linkage Disequilibrium report for AZI1 (79163393 - 79196799 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 5 variations for AZI1
         4 CNVs: 73071 30032 4048 30855
         1 Indel: 25954

    SABiosciences Cancer Mutation PCR Assays
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    DNA2.0 Custom Variant and Variant Library Synthesis for AZI1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    AZI1 for disorders           About MalaCards

    AZI1 for disorders           About GeneDecksing

    OMIM gene information: 613479    OMIM disorders: --


    Export disorders for AZI1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for AZI1 gene, integrated from 9 sources (see all 34):
    (articles sorted by number of sources associating them with AZI1)
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    1. Proteomic characterization of the human centrosome by protein correlation profiling. (PubMed id 14654843)1, 2, 3 Andersen J.S....Mann M. (2003)
    2. Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10470851)1, 2, 3 Kikuno R....Ohara O. (1999)
    3. A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)1, 2 Beausoleil S.A.... Gygi S.P. (2006)
    4. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)1, 2 Olsen J.V....Mann M. (2006)
    5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    6. Genomic organization of the mouse AZ1 gene that encodes the protein localized to preacrosomes of spermatids. (PubMed id 9070930)1, 3 Aoto H....Tajima S. (1997)
    7. ODCp, a brain- and testis-specific ornithine decarboxylase paralogue, functions as an antizyme inhibitor, although less efficiently than AzI1. (PubMed id 18062773)1, 9 Snapir Z....Kahana C. (2008)
    8. Regulation of CEP131 gene expression by SP1. (PubMed id 23137637)1 Huong P.T....Kim B.Y. (2013)
    9. Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
    10. A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 22994 HGNC: 29511 AceView: AZI1 Ensembl:ENSG00000141577 euGenes: HUgn22994
    ECgene: AZI1 H-InvDB: AZI1

    (According to HUGE)
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    HUGE: KIAA1118

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for AZI1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for AZI1 gene:
    Search GeneIP for patents involving AZI1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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