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Aliases for ATG4A Gene

Aliases for ATG4A Gene

  • Autophagy Related 4A, Cysteine Peptidase 2 3
  • APG4A 3 4 6
  • AUTL2 3 4 6
  • Autophagy-Related Cysteine Endopeptidase 2 3 4
  • Autophagy-Related Protein 4 Homolog A 3 4
  • AUT-Like 2 Cysteine Endopeptidase 3 4
  • Autophagin-2 3 4
  • HAPG4A 3 4
  • AUT-Like 2, Cysteine Endopeptidase (S. Cerevisiae) 2
  • ATG4 Autophagy Related 4 Homolog A (S. Cerevisiae) 2
  • APG4 Autophagy 4 Homolog A (S. Cerevisiae) 2
  • ATG4 Autophagy Related 4 Homolog A 3
  • AUT-Like 2, Cysteine Endopeptidase 3
  • APG4 Autophagy 4 Homolog A 3
  • Cysteine Protease ATG4A 3
  • Autophagin 2 3
  • EC 3.4.22.- 4
  • EC 3.4.22 63

External Ids for ATG4A Gene

Previous HGNC Symbols for ATG4A Gene

  • AUTL2
  • APG4A

Previous GeneCards Identifiers for ATG4A Gene

  • GC0XP107142
  • GC0XP107221
  • GC0XP107334
  • GC0XP096957

Summaries for ATG4A Gene

Entrez Gene Summary for ATG4A Gene

  • Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

GeneCards Summary for ATG4A Gene

ATG4A (Autophagy Related 4A, Cysteine Peptidase) is a Protein Coding gene. Among its related pathways are Regulation of autophagy. GO annotations related to this gene include cysteine-type peptidase activity. An important paralog of this gene is ATG4B.

UniProtKB/Swiss-Prot for ATG4A Gene

  • Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Gene Wiki entry for ATG4A Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ATG4A Gene

Genomics for ATG4A Gene

Regulatory Elements for ATG4A Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for ATG4A Gene

Start:
108,091,668 bp from pter
End:
108,154,671 bp from pter
Size:
63,004 bases
Orientation:
Plus strand

Genomic View for ATG4A Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for ATG4A Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ATG4A Gene

Proteins for ATG4A Gene

  • Protein details for ATG4A Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8WYN0-ATG4A_HUMAN
    Recommended name:
    Cysteine protease ATG4A
    Protein Accession:
    Q8WYN0
    Secondary Accessions:
    • A6NCH2
    • B2RAZ7
    • D3DUY0
    • O95534
    • Q5JYY9
    • Q5JYZ0
    • Q86VE5
    • Q96KQ0
    • Q96KQ1

    Protein attributes for ATG4A Gene

    Size:
    398 amino acids
    Molecular mass:
    45378 Da
    Quaternary structure:
    No Data Available

    Three dimensional structures from OCA and Proteopedia for ATG4A Gene

    Alternative splice isoforms for ATG4A Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ATG4A Gene

Proteomics data for ATG4A Gene at MOPED

Post-translational modifications for ATG4A Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for ATG4A Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for ATG4A (ATG4A)

Domains for ATG4A Gene

Protein Domains for ATG4A Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for ATG4A Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q8WYN0

UniProtKB/Swiss-Prot:

ATG4A_HUMAN :
  • Q8WYN0
Family:
  • Belongs to the peptidase C54 family.
genes like me logo Genes that share domains with ATG4A: view

No data available for Gene Families for ATG4A Gene

Function for ATG4A Gene

Molecular function for ATG4A Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=33.9 uM for MAP1LC3B {ECO:0000269 PubMed:21177865, ECO:0000269 PubMed:22302004}; KM=20.8 uM for GABARAP {ECO:0000269 PubMed:21177865, ECO:0000269 PubMed:22302004}; KM=36.7 uM for GABARAPL1 {ECO:0000269 PubMed:21177865, ECO:0000269 PubMed:22302004}; KM=15.7 uM for GABARAPL2 {ECO:0000269 PubMed:21177865, ECO:0000269 PubMed:22302004};
UniProtKB/Swiss-Prot EnzymeRegulation:
Inhibited by N-ethylmaleimide. Redox-regulated during autophagy since reducing conditions activate ATG4A whereas an oxidizing environment such as the presence of H(2)O(2) inhibits its activity.
UniProtKB/Swiss-Prot Function:
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Enzyme Numbers (IUBMB) for ATG4A Gene

Gene Ontology (GO) - Molecular Function for ATG4A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004197 cysteine-type endopeptidase activity IBA --
GO:0005515 protein binding IPI 25416956
GO:0008234 cysteine-type peptidase activity IDA 12473658
genes like me logo Genes that share ontologies with ATG4A: view

Phenotypes for ATG4A Gene

GenomeRNAi human phenotypes for ATG4A:
genes like me logo Genes that share phenotypes with ATG4A: view

Animal Model Products

miRNA for ATG4A Gene

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for ATG4A

In Situ Assay Products

Flow Cytometry Products

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for ATG4A Gene

Localization for ATG4A Gene

Subcellular locations from UniProtKB/Swiss-Prot for ATG4A Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for ATG4A Gene COMPARTMENTS Subcellular localization image for ATG4A gene
Compartment Confidence
cytosol 2
nucleus 2
vacuole 2
cytoskeleton 1
mitochondrion 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for ATG4A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm ISS 12446702
GO:0005829 cytosol IBA --
genes like me logo Genes that share ontologies with ATG4A: view

Pathways for ATG4A Gene

SuperPathways for ATG4A Gene

Superpath Contained pathways
1 Regulation of autophagy
genes like me logo Genes that share pathways with ATG4A: view

Pathways by source for ATG4A Gene

1 KEGG pathway for ATG4A Gene

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for ATG4A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000045 autophagic vacuole assembly IBA --
GO:0000422 mitochondrion degradation IBA --
GO:0006501 C-terminal protein lipidation IBA --
GO:0006508 proteolysis IDA 12473658
GO:0006612 protein targeting to membrane IBA --
genes like me logo Genes that share ontologies with ATG4A: view

Compounds for ATG4A Gene

(8) ApexBio Compounds for ATG4A Gene

Compound Action Cas Number
Chloroquine diphosphate Antimalarial drug [50-63-5]
Dexamethasone (DHAP) Glucocorticoidan;ti-inflammatory [1950-2-2]
Divalproex Sodium Epilepsy/migraines/bipolar disorder medicine [76584-70-8]
Nimodipine [66085-59-4]
Nocodazole "Tubulin production inhibitor,anti-neoplastic agent" [31430-18-9]
Omeprazole "H+,K+-ATPase inhibitor" [73590-58-6]
Sulfacetamide Sodium Sulfonamide antibiotic [127-56-0]
Trifluoperazine 2HCl [440-17-5]
genes like me logo Genes that share compounds with ATG4A: view

Transcripts for ATG4A Gene

Unigene Clusters for ATG4A Gene

Autophagy related 4A, cysteine peptidase:
Representative Sequences:

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for ATG4A

Primer Products

  • QuantiTect SYBR Green Assays in human,mouse,rat
  • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
  • QuantiFast Probe-based Assays in human,mouse,rat

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for ATG4A Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f · 1g ^ 2 ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14 ^ 15
SP1: - - - -
SP2: - - - - - -
SP3: - - - - - -
SP4: - - - -
SP5: - - - - -
SP6: - -
SP7: - -
SP8:
SP9: -

Relevant External Links for ATG4A Gene

GeneLoc Exon Structure for
ATG4A
ECgene alternative splicing isoforms for
ATG4A

Expression for ATG4A Gene

mRNA expression in normal human tissues for ATG4A Gene

Protein differential expression in normal tissues for ATG4A Gene

This gene is overexpressed in Saliva (37.1), Pancreas (19.5), and Gallbladder (9.4).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for ATG4A Gene

SOURCE GeneReport for Unigene cluster for ATG4A Gene Hs.8763

mRNA Expression by UniProt/SwissProt for ATG4A Gene

Q8WYN0-ATG4A_HUMAN
Tissue specificity: Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
genes like me logo Genes that share expressions with ATG4A: view

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Expression partners for ATG4A Gene

Orthologs for ATG4A Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for ATG4A Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia ATG4A 35
  • 92.63 (n)
  • 95.23 (a)
ATG4A 36
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia ATG4A 35
  • 92.38 (n)
  • 94.22 (a)
ATG4A 36
  • 93 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Atg4a 35
  • 90.44 (n)
  • 93.15 (a)
Atg4a 16
Atg4a 36
  • 93 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia ATG4A 35
  • 99.66 (n)
  • 99.5 (a)
ATG4A 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Atg4al1 35
  • 91.54 (n)
  • 93.22 (a)
oppossum
(Monodelphis domestica)
Mammalia ATG4A 36
  • 76 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia ATG4A 36
  • 80 (a)
OneToOne
chicken
(Gallus gallus)
Aves ATG4A 35
  • 76.68 (n)
  • 81.98 (a)
ATG4A 36
  • 81 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ATG4A 36
  • 78 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia atg4a 35
  • 73.14 (n)
  • 75.47 (a)
Str.4065 35
zebrafish
(Danio rerio)
Actinopterygii atg4a 35
  • 69.25 (n)
  • 75.62 (a)
atg4a 36
  • 74 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008497 35
  • 51.55 (n)
  • 46.27 (a)
fruit fly
(Drosophila melanogaster)
Insecta Atg4 35
  • 52.89 (n)
  • 46.81 (a)
Atg4 36
  • 37 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea atg-4.1 35
  • 50.38 (n)
  • 45.11 (a)
atg-4.1 36
  • 33 (a)
OneToMany
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ABL191W 35
  • 45.77 (n)
  • 33.21 (a)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0D19536g 35
  • 48.27 (n)
  • 37.04 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ATG4 35
  • 45.48 (n)
  • 33.46 (a)
ATG4 36
  • 19 (a)
OneToMany
thale cress
(Arabidopsis thaliana)
eudicotyledons AT3G59950 35
  • 47.35 (n)
  • 40.28 (a)
rice
(Oryza sativa)
Liliopsida Os04g0682000 35
  • 46.1 (n)
  • 40.19 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.2997 36
  • 40 (a)
OneToMany
Species with no ortholog for ATG4A:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ATG4A Gene

ENSEMBL:
Gene Tree for ATG4A (if available)
TreeFam:
Gene Tree for ATG4A (if available)

Paralogs for ATG4A Gene

Paralogs for ATG4A Gene

Selected SIMAP similar genes for ATG4A Gene using alignment to 5 proteins:

Pseudogenes.org Pseudogenes for ATG4A Gene

genes like me logo Genes that share paralogs with ATG4A: view

Variants for ATG4A Gene

Sequence variations from dbSNP and Humsavar for ATG4A Gene

SNP ID Clin Chr 0X pos Sequence Context AA Info Type MAF
rs10713 -- 108,154,630(-) tcaaa(A/C/T)aaaaa utr-variant-3-prime
rs717710 -- 108,142,276(-) CATGC(A/G)CCACC intron-variant
rs717711 -- 108,141,875(-) TAATA(A/G)CCATG intron-variant
rs807177 -- 108,104,617(+) tttct(C/G)agtgt intron-variant
rs807183 -- 108,094,263(+) tgaga(A/G)tctaa intron-variant

Relevant External Links for ATG4A Gene

HapMap Linkage Disequilibrium report
ATG4A

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for ATG4A Gene

Disorders for ATG4A Gene

Relevant External Links for ATG4A

Genetic Association Database (GAD)
ATG4A
Human Genome Epidemiology (HuGE) Navigator
ATG4A

No disorders were found for ATG4A Gene.

No data available for MalaCards , OMIM , UniProtKB/Swiss-Prot , University of Copenhagen DISEASES , Novoseek inferred disease relationships and Genatlas for ATG4A Gene

Publications for ATG4A Gene

  1. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy. (PMID: 12446702) Marino G. … Lopez-Otin C. (J. Biol. Chem. 2003) 2 3 4
  2. The COOH terminus of GATE-16, an intra-Golgi transport modulator, is cleaved by the human cysteine protease HsApg4A. (PMID: 12473658) Scherz-Shouval R. … Elazar Z. (J. Biol. Chem. 2003) 2 3 4
  3. Reactive oxygen species are essential for autophagy and specifically regulate the activity of Atg4. (PMID: 17347651) Scherz-Shouval R. … Elazar Z. (EMBO J. 2007) 3 4
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4
  5. LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation. (PMID: 15169837) Kabeya Y. … Yoshimori T. (J. Cell Sci. 2004) 3 4

Products for ATG4A Gene

  • Addgene plasmids for ATG4A

Sources for ATG4A Gene

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