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Aliases for ATG3 Gene

Aliases for ATG3 Gene

  • Autophagy Related 3 2 3
  • Autophagy-Related Protein 3 3 4
  • APG3-LIKE 3 4
  • APG3L 3 4
  • HApg3 3 4
  • APG3 3 4
  • ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) 2
  • Ubiquitin-Like-Conjugating Enzyme ATG3 3
  • APG3 Autophagy 3-Like (S. Cerevisiae) 2
  • ATG3 Autophagy Related 3 Homolog 3
  • Protein PC3-96 4
  • 2610016C12Rik 3
  • EC 6.3.2.- 4
  • PC3-96 3

External Ids for ATG3 Gene

Previous HGNC Symbols for ATG3 Gene

  • APG3L

Previous GeneCards Identifiers for ATG3 Gene

  • GC03M113735
  • GC03M112251
  • GC03M109636

Summaries for ATG3 Gene

Entrez Gene Summary for ATG3 Gene

  • This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

GeneCards Summary for ATG3 Gene

ATG3 (Autophagy Related 3) is a Protein Coding gene. Among its related pathways are Senescence and Autophagy and Regulation of autophagy. GO annotations related to this gene include enzyme binding and Atg12 transferase activity.

UniProtKB/Swiss-Prot for ATG3 Gene

  • E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ATG3 Gene

Genomics for ATG3 Gene

Regulatory Elements for ATG3 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for ATG3 Gene

Start:
112,532,507 bp from pter
End:
112,562,046 bp from pter
Size:
29,540 bases
Orientation:
Minus strand

Genomic View for ATG3 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for ATG3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ATG3 Gene

Proteins for ATG3 Gene

  • Protein details for ATG3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NT62-ATG3_HUMAN
    Recommended name:
    Ubiquitin-like-conjugating enzyme ATG3
    Protein Accession:
    Q9NT62
    Secondary Accessions:
    • Q6PKC5
    • Q9H6L9

    Protein attributes for ATG3 Gene

    Size:
    314 amino acids
    Molecular mass:
    35864 Da
    Quaternary structure:
    • Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Interacts with FNBP1L.
    SequenceCaution:
    • Sequence=AAH02830.1; Type=Frameshift; Positions=290; Evidence={ECO:0000305}; Sequence=BAB15237.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for ATG3 Gene

    Alternative splice isoforms for ATG3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ATG3 Gene

Proteomics data for ATG3 Gene at MOPED

Post-translational modifications for ATG3 Gene

  • Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis.
  • Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy
  • Ubiquitination at Lys11
  • Modification sites at PhosphoSitePlus

Other Protein References for ATG3 Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for ATG3 (ATG3)

No data available for DME Specific Peptides for ATG3 Gene

Domains for ATG3 Gene

Protein Domains for ATG3 Gene

Suggested Antigen Peptide Sequences for ATG3 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9NT62

UniProtKB/Swiss-Prot:

ATG3_HUMAN :
  • Q9NT62
Family:
  • Belongs to the ATG3 family.
genes like me logo Genes that share domains with ATG3: view

No data available for Gene Families for ATG3 Gene

Function for ATG3 Gene

Molecular function for ATG3 Gene

UniProtKB/Swiss-Prot Function: E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Enzyme Numbers (IUBMB) for ATG3 Gene

Gene Ontology (GO) - Molecular Function for ATG3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 11825910
GO:0016874 ligase activity IEA --
GO:0019776 Atg8 ligase activity ISS --
GO:0019777 Atg12 transferase activity ISS --
GO:0019787 ubiquitin-like protein transferase activity IDA 11825910
genes like me logo Genes that share ontologies with ATG3: view
genes like me logo Genes that share phenotypes with ATG3: view

Animal Model Products

CRISPR Products

miRNA for ATG3 Gene

miRTarBase miRNAs that target ATG3

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for ATG3

In Situ Assay Products

Flow Cytometry Products

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for ATG3 Gene

Localization for ATG3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ATG3 Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for ATG3 Gene COMPARTMENTS Subcellular localization image for ATG3 gene
Compartment Confidence
cytosol 5
plasma membrane 3
vacuole 3
lysosome 1
mitochondrion 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for ATG3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000153 cytoplasmic ubiquitin ligase complex IPI 11825910
GO:0005829 cytosol IDA 11825910
genes like me logo Genes that share ontologies with ATG3: view

Pathways for ATG3 Gene

genes like me logo Genes that share pathways with ATG3: view

Pathways by source for ATG3 Gene

1 BioSystems pathway for ATG3 Gene
1 KEGG pathway for ATG3 Gene

PCR Array Products

Gene Ontology (GO) - Biological Process for ATG3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000045 autophagic vacuole assembly ISS --
GO:0000422 mitochondrion degradation IBA --
GO:0006464 cellular protein modification process IDA 11825910
GO:0006612 protein targeting to membrane ISS --
GO:0006914 autophagy --
genes like me logo Genes that share ontologies with ATG3: view

Transcripts for ATG3 Gene

Unigene Clusters for ATG3 Gene

Autophagy related 3:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for ATG3

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for ATG3 Gene

ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13
SP1: -
SP2:
SP3: - -
SP4: - -
SP5: -

Relevant External Links for ATG3 Gene

GeneLoc Exon Structure for
ATG3
ECgene alternative splicing isoforms for
ATG3

Expression for ATG3 Gene

mRNA expression in normal human tissues for ATG3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for ATG3 Gene

SOURCE GeneReport for Unigene cluster for ATG3 Gene Hs.477126

mRNA Expression by UniProt/SwissProt for ATG3 Gene

Q9NT62-ATG3_HUMAN
Tissue specificity: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
genes like me logo Genes that share expressions with ATG3: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for ATG3 Gene

Orthologs for ATG3 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for ATG3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia ATG3 35
  • 99.47 (n)
  • 100 (a)
ATG3 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia ATG3 35
  • 95.01 (n)
  • 98.09 (a)
ATG3 36
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia -- 36
  • 97 (a)
OneToMany
-- 36
  • 77 (a)
OneToMany
ATG3 35
  • 94.09 (n)
  • 97.74 (a)
mouse
(Mus musculus)
Mammalia Atg3 35
  • 92.36 (n)
  • 97.45 (a)
Atg3 16
Atg3 36
  • 97 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia ATG3 36
  • 89 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 61 (a)
OneToMany
-- 36
  • 88 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Atg3 35
  • 90.55 (n)
  • 96.82 (a)
chicken
(Gallus gallus)
Aves ATG3 35
  • 84.56 (n)
  • 92.65 (a)
ATG3 36
  • 93 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ATG3 36
  • 91 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.12629 35
tropical clawed frog
(Silurana tropicalis)
Amphibia atg3 35
  • 79.96 (n)
  • 88.24 (a)
Str.602 35
zebrafish
(Danio rerio)
Actinopterygii apg3l 35
  • 77.07 (n)
  • 84.39 (a)
apg3l 36
  • 84 (a)
OneToOne
wufb15g12 35
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP011582 35
  • 60.86 (n)
  • 62.9 (a)
fruit fly
(Drosophila melanogaster)
Insecta Aut1 35
  • 63.46 (n)
  • 66.67 (a)
Aut1 36
  • 63 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea atg-3 35
  • 58.11 (n)
  • 55.82 (a)
atg-3 36
  • 53 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ATG3 36
  • 35 (a)
OneToOne
ATG3 38
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.6805 35
soybean
(Glycine max)
eudicotyledons Gma.1721 35
thale cress
(Arabidopsis thaliana)
eudicotyledons ATG3 35
  • 57.61 (n)
  • 49.64 (a)
barley
(Hordeum vulgare)
Liliopsida Hv.2402 35
corn
(Zea mays)
Liliopsida Zm.4250 35
rice
(Oryza sativa)
Liliopsida Os.27665 35
Os01g0200000 35
  • 57.5 (n)
  • 51.3 (a)
wheat
(Triticum aestivum)
Liliopsida Ta.21403 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.1702 36
  • 66 (a)
OneToOne
Species with no ortholog for ATG3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)

Evolution for ATG3 Gene

ENSEMBL:
Gene Tree for ATG3 (if available)
TreeFam:
Gene Tree for ATG3 (if available)

Paralogs for ATG3 Gene

Pseudogenes.org Pseudogenes for ATG3 Gene

genes like me logo Genes that share paralogs with ATG3: view

No data available for Paralogs for ATG3 Gene

Variants for ATG3 Gene

Sequence variations from dbSNP and Humsavar for ATG3 Gene

SNP ID Clin Chr 03 pos Sequence Context AA Info Type MAF
rs724125 -- 112,551,459(+) AGCCA(A/G)AAGTG intron-variant
rs923695 -- 112,540,650(+) ACCCA(C/T)GGAAC intron-variant
rs958935 -- 112,551,565(+) CTAAA(A/G)AAAGA intron-variant
rs971832 -- 112,553,800(+) ATCAC(A/T)GGGCA intron-variant
rs1388442 -- 112,538,444(+) CTTCT(A/G)TAACT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for ATG3 Gene

Variant ID Type Subtype PubMed ID
nsv877342 CNV Gain 21882294
esv272571 CNV Insertion 20981092

Relevant External Links for ATG3 Gene

HapMap Linkage Disequilibrium report
ATG3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ATG3 Gene

Disorders for ATG3 Gene

Relevant External Links for ATG3

Genetic Association Database (GAD)
ATG3
Human Genome Epidemiology (HuGE) Navigator
ATG3
genes like me logo Genes that share disorders with ATG3: view

No data available for UniProtKB/Swiss-Prot for ATG3 Gene

Publications for ATG3 Gene

  1. Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. (PMID: 11825910) Tanida I. … Kominami E. (J. Biol. Chem. 2002) 2 3 4 23
  2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4
  3. Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry. (PMID: 15144186) Brill L.M. … Peters E.C. (Anal. Chem. 2004) 3 4
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4
  5. Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. (PMID: 15592455) Rush J. … Comb M.J. (Nat. Biotechnol. 2005) 3 4

Products for ATG3 Gene

Sources for ATG3 Gene

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