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ATAD2 Gene

protein-coding   GIFtS: 53

GC08M124401
ATPase family, AAA domain containing 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
ANCCA 2, 3, 5
DKFZp667N1320 1, 2
EC 3.6.1.3 3
MGC131938 2
MGC142216 2
MGC29843 1, 2
MGC5254 1, 2
PRO2000 1, 2
Descriptions
AAA nuclear coregulator cancer-associated protein 3
ATPase family, AAA domain containing 2 2
External Ids
HGNC: 301231
Entrez Gene: 290282
UniProtKB: Q6PL183
Ensembl: ENSG000001568027
Search outside databases for aliases for ATAD2 gene

Previous GC identifer: GC08M124292

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for ATAD2:
A large family of ATPases has been described, whose key feature is that they share a conserved
region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to
this family either contain one or two AAA (ATPases Associated with diverse cellular Activities)
domains. AAA family proteins often perform chaperone-like functions that assist in the assembly,
operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA
domains, as well as a bromodomain. [provided by RefSeq]

UniProtKB/Swiss-Prot: ATAD2_HUMAN, Q6PL18
Function: May be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the
expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in
the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for
histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle
progression of breast cancer cells

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ATAD2 gene  

Entrez Gene cytogenetic band: 8q24.13   Ensembl cytogenetic band:  8q24.13   HGNC cytogenetic band: 8q24.13

ATAD2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M124401:     (about GC identifiers)

Start:
124,401,922 bp from pter
End:
124,477,868 bp from pter
Size:
75,947 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000008.9  NT_008046.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: ATAD2_HUMAN, Q6PL18 (See protein sequence)
Recommended Name: ATPase family AAA domain-containing protein 2  
Size: 1390 amino acids; 158554 Da
Subunit: Interacts with ESR1 and NCOA3 and these interactions are enhanced by estradiol
Subcellular location: Nucleus
Sequence caution: Sequence=AAH10686.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence.
Sequence of unknown origin in the N-terminal part (676-679);
PDB structures from and Proteopedia :
3DAI (3D)    
Secondary accessions: Q14CR1 Q658P2 Q68CQ0 Q6PJV6 Q8N890 Q9UHS5
Alternative splicing: 2 isoforms:  Q6PL18-1   Q6PL18-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_054828.2  

    ENSEMBL proteins: 
    ENSP00000287394 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (ATAD2)
    Human Recombinant Proteins from Abnova (ATAD2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for ATAD2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for ATAD2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (ATAD2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (ATAD2)
    Novus Biologicals Antibodies for ATAD2

    Assays for ATAD2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/6 InterPro domains/families (see all 6 ):
     IPR003959 ATPase_AAA_core
     IPR003960 ATPase_AAA_CS
     IPR018359 Bromodomain_CS
     IPR001487 Bromodomain
     IPR003593 ATPase_AAA+_core


       GeneDecks  ATAD2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q6PL18

    ProtoNet protein and cluster: Q6PL18

    2 Blocks protein families:
    IPB001487 Bromodomain signature
    IPB003960 AAA-protein subdomain


    UniProtKB/Swiss-Prot: ATAD2_HUMAN, Q6PL18
    Similarity: Belongs to the AAA ATPase family
    Similarity: Contains 1 bromo domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ATAD2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (ATAD2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014109

                  Applied Biosystems Silencer® siRNAs for ATAD2

                  Sigma-Aldrich siRNA for ATAD2  
                         Sigma-Aldrich shRNA for ATAD2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for ATAD2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_014109
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_014109
                                     untagged cDNA clones in CMV expression vector (see all 2): BC007123 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_014109

    UniProtKB/Swiss-Prot: ATAD2_HUMAN, Q6PL18
    Function: May be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the
    expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in
    the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for
    histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle
    progression of breast cancer cells
    Catalytic activity: ATP + H(2)O = ADP + phosphate
    Induction: Up-regulated in breast, uterus, colon, ovary, and stomach tumors. Induced in breast
    cancer cells overexpressing NCOA3 or treated with estrogen. Down-regulated in
    5-fluorouracil-resistant derivatives of the colon cancer cell line HCT116
    Enzyme Number (IUBMB): EC 3.6.1.3 

    5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0003700 transcription factor activity IEA--
    GO:0005524 ATP binding IEA--
    GO:0016787 hydrolase activity IEA--
    GO:0017111 nucleoside-triphosphatase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    2 Interacting proteins for ATAD2 (ENSP000002873943) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ESR1ENSP000002062493STRING (score=.734)
    --ENSP000003610633STRING (score=.813)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for ATAD2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ATAD2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (ATAD2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014109

                  Sigma-Aldrich siRNA for ATAD2  
                         Sigma-Aldrich shRNA for ATAD2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_014109  

    REFSEQ mRNAs for ATAD2 gene: 

    NM_014109.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_014109  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_014109
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_014109
                                     untagged cDNA clones in CMV expression vector (see all 2): BC007123 

    Additional cDNA sequence: 

    AF118088.1 AK097133.1 AL833653.1 AY598335.1 BC010686.1 BC019909.1 BC112942.1 BC113656.1 
    BC143716.1 CR749832.1 

    12 DOTS entries:

    DT.441446  DT.100777335  DT.91750028  DT.100777333  DT.121485529  DT.422400  DT.100655985  DT.121485560 
    DT.91734891  DT.91862825  DT.92022435  DT.95367627 

    24/170 AceView cDNA sequences (see all 170 ):

    AA808229 AA995158 AI133393 AL833653 AA287933 BX643073 BQ425008 AA897632 
    CB120155 CB159561 BC007123 AF118088 NM_014109 AK097133 AW168205 BQ896166 
    AW151919 CB161159 CD244182 AI240868 BI861220 BM014842 BP356240 BM559313 

    highest scoring ESTs for ATAD2:

    AA451665 AA355899 AA455762 AA522645 AA974408 AA995158 AL556876 BE504612 BE541451 BF062930 

    Unigene Cluster for ATAD2:

    ATPase family, AAA domain containing 2
    Hs.370834  [show with all ESTs]
    Unigene Representative Sequence: NM_014109


    GeneLoc Exon Structure

    5/12 Alternative Splicing Database (ASD) splice patterns (SP) for ATAD2 (see all 12 )

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11a · 11b ^ 12 ^ 13a · 13b ^ 14a · 14b ^ 15 ^ 16a · 16b · 16c · 16d ^ 17a ·
    SP1:                          -                                                                                                                                 
    SP2:                                                                                                                                                            
    SP3:                                                                                                                                                            
    SP4:                                                                                                                                                            
    SP5:                                                                                                                                                            

    ExUns: 17b · 17c ^ 18a · 18b ^ 19a · 19b ^ 20 ^ 21a · 21b · 21c ^ 22a · 22b ^ 23 ^ 24a · 24b ^ 25
    SP1:                                                                                                
    SP2:                                                                                                
    SP3:                                                                                                
    SP4:                                                                                                
    SP5:                                                                                                

    About this scheme

    ECgene alternative splicing isoforms for ATAD2

    1 Ensembl transcript including schematic representation:
    ENST00000287394  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    ATAD2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for ATAD2

    1 / 2 / 3

    13 probe-sets matching ATAD2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    58354_at2, 3 U95-B 1 1.00 1.00 0.88 1.23 AI656807 0.60 1.00 0.82 1

    62155_r_at2, 3 U95-C 1 1.00 1.00 0.71 0.84 AA974408 0.80 1.00 0.91 1

    61978_at2, 3 U95-C 1 1.00 1.00 0.47 0.23 AI139629 0.60 1.00 0.82 1

    87339_at2, 3 U95-E 1 1.00 1.00 0.22 1.08 AA451665 1.00 1.00 1.00 1

    61977_s_at2, 3 U95-C 2 1.00 0.97 -0.50 1.02 AA522645 0.80 1.00 0.91 1

    218782_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_014109 0.60 1.00 0.82 1

    222740_at2, 3 U133-B 1 1.00 1.00 -- -- AI925583 0.60 1.00 0.82 1

    235266_at2, 3 U133-B 1 1.00 1.00 -- -- AI139629 0.60 1.00 0.82 1

    228401_at2, 3 U133-B 1 0.82 1.00 -- -- AI656807 0.60 1.00 0.82 1

    218782_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    222740_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    235266_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    228401_at2 U133Plus2 1 0.82 1.00 -- -- -- -- -- -- --
    GeneDecks  ATAD2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: ATTTGCGGGA

    SOURCE GeneReport for Unigene cluster: Hs.370834

    UniProtKB/Swiss-Prot: ATAD2_HUMAN, Q6PL18
    Tissue specificity: Highly expressed in estrogen receptor positive breast tumors and in
    osteosarcoma tumors

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for ATAD2 gene from 5/15 species (see all 15 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    ATAD21   -- ATPase family, AAA domain containing 2 91.78(n)
    92.77(a)
    475090  XM_845427.1  XP_850520.1 
    chimpanzee
    (Pan troglodytes)
    ATAD21   -- ATPase family, AAA domain containing 2 99.33(n)
    99.06(a)
    464369  XM_001148894.1  XP_001148894.1 
    cow
    (Bos taurus)
    ATAD21   -- ATPase family, AAA domain containing 2 90.88(n)
    91.76(a)
    522920  XM_864276.2  XP_869369.2 
    rat
    (Rattus norvegicus)
    Atad21   -- ATPase family, AAA domain containing 2 84.28(n)
    83.05(a)
    314993  XM_235326.4  XP_235326.4 
    mouse
    (Mus musculus)
    Atad21, 5 155
    ATPase family, AAA domain containing 21, 5 85.37(n)1
    85.15(a)1
    704721  NM_027435.21  NP_081711.21 
     AI1587085  AI4280875  (see all 14)
    About this table        Species with no ortholog for ATAD2

    ENSEMBL Gene Tree for ATAD2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for ATAD2 gene
    ATAD2B2  ATAD12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/336 NCBI SNPs in ATAD2 are shown (see all 336 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 72)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 8 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs90211,2
    A,C,F,H,O124402081(-) TCCCTT/CGTCTT 1 -- ut31 ese38Minor allele frequency- C:0.31MN EU EA WA NA 972
    rs37581221,2
    C,F,H124409640(-) GATAAT/CACATA 1 T/I mis1 ese38Minor allele frequency- C:0.00EU EA WA 812
    rs69938991,2
    F,H124478265(+) GCCATC/ACAGGA 1 -- ng314Minor allele frequency- A:0.01EU EA WA 418
    rs48708491,2
    C,H124477949(+) CCACCA/GTAGAA 1 -- ng314Minor allele frequency- G:0.00EU EA WA 418
    --
    rs70000341,2
    C124479768(+) tgcctA/Cagcct 1 -- ng310--------
    --
    rs168982631,2
    F124479323(+) GAAGAA/GGAGAG 1 -- ng31 trp33Minor allele frequency- G:0.01NA 134
    rs150601,2
    C124402103(+) GATGGG/TAGGAG 1 -- ut31 trp31Minor allele frequency- T:0.00MN 184
    --
    rs37799821,2
    C124478661(-) TTTTTC/GAGACA 1 -- ng310--------
    --
    rs40065091,2
    H124401882(-) TGTGTT/AAATAA 1 -- ut314Minor allele frequency- A:0.00EU EA WA 418
    rs168982481,2
    C,F,H124428586(+) TTGTCA/CACAGT 1 V/V syn1 ese311Minor allele frequency- C:0.05NA EU EA WA 978
    About this table

    HapMap Linkage Disequilibrium images for ATAD2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 611941

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/16 PubMed articles for ATAD2 gene (see all 16 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 29028 HGNC: 30123 AceView: ATAD2 Ensembl:ENSG00000156802 euGenes: HUgn29028
    ECgene: ATAD2 H-InvDB: ATAD2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for ATAD2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for ATAD2 Antibodies for ATAD2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for ATAD2 Browse small molecules at Sigma-Aldrich
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