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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ASPG Gene

protein-coding   GIFtS: 44
GCID: GC14P104552

asparaginase homolog (S. cerevisiae)

(Previous name: chromosome 14 open reading frame 76 )
(Previous symbol: C14orf76)
 Explore 3 diseases affiliated with
ASPG via our new
 Human Malady Compendium 
Biological research products
for ASPG
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Asparaginase Homolog (S. Cerevisiae)1 2     GPA/WT2
C14orf761 2 3     Wild-Type L-Asparaginase2
Chromosome 14 Open Reading Frame 761     EC 3.1.1.53
60 KDa Lysophospholipase2     

External Ids:    HGNC: 201231   Entrez Gene: 3745692   Ensembl: ENSG000001661837   UniProtKB: Q86U103   

Export aliases for ASPG gene to outside databases

Previous GC identifers: GC14P103622 GC14P084732


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: LPP60_HUMAN, Q86U10
Function: Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000014.8  NC_018925.1  NT_026437.12  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ASPG gene promoter:
         FOXF2   NRSF form 1   ATF-2   NRSF form 2   LCR-F1   HNF-4alpha1   CREB   CRE-BP1   deltaCREB   c-Myb   
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search SABiosciences Chromatin IP Primers for ASPG

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ASPG


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 14q32.33   Ensembl cytogenetic band:  14q32.33   HGNC cytogenetic band: 14q32.33

ASPG Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ASPG gene location

GeneLoc information about chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14P104552:  view genomic region     (about GC identifiers)

Start:
104,552,016 bp from pter      End:
104,579,098 bp from pter
Size:
27,083 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: LPP60_HUMAN, Q86U10 (See protein sequence)
Recommended Name: 60 kDa lysophospholipase  
Size: 573 amino acids; 60883 Da
Subunit: Monomer (By similarity)
Sequence caution: Sequence=CAD62331.1; Type=Erroneous translation; Note=Wrong choice of frame;
Secondary accessions: B9EGQ2 Q8IV80
Alternative splicing: 2 isoforms:  Q86U10-1   Q86U10-3   (May be due to a competing acceptor splice site)

Explore the universe of human proteins at neXtProt for ASPG: NX_Q86U10

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q86U10

  • ASPG Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_001073933.2  
    ENSEMBL proteins: 
     ENSP00000450040   ENSP00000448911   ENSP00000447032   ENSP00000446856   ENSP00000389003  

    Human Recombinant Protein Products: 
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    Uscn Proteins for ASPG

    Gene Ontology (GO): 1 cellular component term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005625soluble fraction ----


    ASPG for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for ASPG


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ASPG for domains           About GeneDecksing

    5 InterPro domains/families:
     IPR020683 Ankyrin_rpt-contain_dom
     IPR020827 Asparaginase/glutaminase_CS
     IPR006034 Asparaginase/glutaminase
     IPR006033 AsnASEI
     IPR002110 Ankyrin_rpt

    Graphical View of Domain Structure for InterPro Entry Q86U10

    ProtoNet protein and cluster: Q86U10

    1 Blocks protein family: IPB002110 Ankyrin repeat signature

    UniProtKB/Swiss-Prot: LPP60_HUMAN, Q86U10
    Similarity: In the N-terminal section; belongs to the asparaginase 1 family
    Similarity: Contains 5 ANK repeats


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: LPP60_HUMAN, Q86U10
    Function: Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities
    Catalytic activity: 2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate
    Catalytic activity: L-asparagine + H(2)O = L-aspartate + NH(3)
    Catalytic activity: 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine +
    acetate

    Enzyme Number (IUBMB): EC 3.1.1.51

    miRNA
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    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for ASPG (see all 4)
    OriGene shRNA RFP: ASPG
    OriGene siRNA: ASPG
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat ASPG

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ASPG

    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:00038471-alkyl-2-acetylglycerophosphocholine esterase activity IEA--
    GO:0004067asparaginase activity IEA--
    GO:0004622lysophospholipase activity IEA--


    ASPG for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1asparagine degradation I
    asparagine degradation I1.00


    1 BioSystems Pathway for ASPG 
        asparagine degradation I


    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ASPG

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006520cellular amino acid metabolic process ----
    GO:0006528asparagine metabolic process IEA--
    GO:0006644phospholipid metabolic process IEA--
    GO:0016042lipid catabolic process IEA--


    ASPG for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ASPG

    10/46 HMDB Compounds for ASPG (see all 46)    About this table
    CompoundSynonyms CAS #PubMed Ids
    Arachidic acidArachate (see all 10)506-30-9--
    Arachidonic acid5,8,11,14-Eicosatetraenoic acid (see all 16)506-32-1--
    Glycerophosphocholine2-[[(2,3-dihydroxypropoxy)hydroxyphosphinyl]oxy]-N,N,N-trimethyl-Ethanaminium inner salt (see all 21)28319-77-9--
    Heptadecanoic acidHeptadecanoate (see all 16)506-12-7--
    Heptadecanoyl CoAHeptadecanoyl coenzyme A (see all 19)3546-17-6--
    L-Asparagine(-)-Asparagine (see all 32)70-47-3--
    LysoPC(0:0/18:0)1-Hydroxy-2-stearoyl-sn-glycero-3-phosphocholine (see all 4)----
    LysoPC(10:0)1-Decanoyllysolecithin (see all 14)22248-63-1--
    LysoPC(14:0)LysoPC(14:0) (see all 9)----
    LysoPC(14:1(9Z))LysoPC(14:1) (see all 17)----
    Search CenterWatch for drugs/clinical trials and news about ASPG / LPP60 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ASPG gene: 
    NM_001080464.2  

    Unigene Cluster for ASPG:

    Asparaginase homolog (S. cerevisiae)
    Hs.146239  [show with all ESTs]
    Unigene Representative Sequence: NM_001080464
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000551177(uc001yop.2 uc001yoq.2 uc001yor.2) ENST00000548372(uc001yoo.2)
    ENST00000546892 ENST00000551170 ENST00000549809 ENST00000552126 ENST00000556267
    ENST00000550583 ENST00000551871 ENST00000455920

    miRNA
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    Inhib. RNA
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    OriGene shRNA RFP: ASPG
    OriGene siRNA: ASPG
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    Clone
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      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ASPG

    Additional cDNA sequence: 

    BC035836.1 BC136637.1 

    6 DOTS entries:

    DT.40267311  DT.100835622  DT.75101104  DT.75185120  DT.91753118  DT.95363552 

    24/37 AceView cDNA sequences (see all 37):

    BX280385 BX282189 BE464276 AW024833 AW304444 CA841092 AI264819 AI436722 
    AI090540 BX247999 BC035836 AX781433 AX781429 AX781428 BX119874 AW269191 
    BX414463 BX407951 AA700625 AX781431 AW025309 AX781430 BM544197 BX410108 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ASPG expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TTTTCAGGAA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    ASPG expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    1 LifeMap In Vivo Development Anatomical Compartment/Cell 
    Tissue Anatomical Compartment CellCategory (developmental path)
    AdiposeInterscapular Brown Adipose DepotAdipose
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization
    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    Definitive endoderm-like cells (A scalable, suspensi...)

    See ASPG Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ASPG

    SOURCE GeneReport for Unigene cluster: Hs.146239
        SABiosciences Custom PCR Arrays for ASPG
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ASPG

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the last universal common ancestor (LUCA).

    Orthologs for ASPG gene from 7/27 species (see all 27)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Aspg1 , 5 asparaginase homolog (S. cerevisiae)1, 5 80.71(n)1
    80.18(a)1
      12 (61.14 cM)5
    1048161  NM_001081169.11  NP_001074638.11 
     1121066835 
    chicken
    (Gallus gallus)
    Aves F1NW85_CHICK6
    Uncharacterized protein
    52(a)
    1 ↔ 1
    5(53149708-53194120)
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    --
    54(a)
    43(a)
    many → 1
    many → 1
    1(5672900-5715670)
    1(5744416-5762969)
    zebrafish
    (Danio rerio)
    Actinopterygii aspg6
    asparaginase homolog (S. cerevisiae)
    43(a)
    1 ↔ 1
    17(45905508-45942116)
    fruit fly
    (Drosophila melanogaster)
    Insecta CG64286
    CG85266
    --
    37(a)
    35(a)
    many → 1
    many → 1
    X(3859459-3862597)
    3R(5627008-5629183)
    worm
    (Caenorhabditis elegans)
    Secernentea C27A7.56
    Protein C27A7.5, isoform a
    32(a)
    1 ↔ 1
    V(12154856-12162280)
    E. coli
    (Escherichia coli)
    Gamma proteobacteria ansA6
    ansB6
    periplasmic L-asparaginase II
    46(a)
    22(a)
    1 ↔ 1
    possible ortholog
    Chromosome(1848884-1849900)
    Chromosome(3097704-3098750)


    ENSEMBL Gene Tree for ASPG (if available)
    TreeFam Gene Tree for ASPG (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/498 NCBI SNPs in ASPG are shown (see all 498    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 14 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1834892121,2
    --104550052(+) CTATAA/GAAAGA 1 -- us2k10--------
    rs1441131461,2
    --104550164(+) ACCAAC/TCTGTG 1 -- us2k10--------
    rs1464851561,2
    --104550262(+) CAGAAA/GACTAA 1 -- us2k10--------
    rs1409477101,2
    --104550372(+) CATGGC/TGGCTC 1 -- us2k10--------
    rs17442751,2
    C,A,--104550468(+) GAAACC/TCCGTC 1 -- us2k10--------
    rs17442761,2
    C,A,--104550471(+) ACCCCC/GTCTCT 1 -- us2k10--------
    rs574287741,2
    C--104550488(+) AAAAA-/AGAAAA 1 -- us2k11Minor allele frequency- A:0.50NA 2
    rs1823237911,2
    --104550544(+) CCCAGC/TTATTC 1 -- us2k10--------
    rs1149940951,2
    F,--104550550(+) TATTCG/AGGGAG 1 -- us2k11Minor allele frequency- A:0.03WA 118
    rs1175815391,2
    F,--104550688(+) GCCTTC/TTGGGG 1 -- us2k12Minor allele frequency- T:0.01NA EA 240

    HapMap Linkage Disequilibrium report for ASPG (104552016 - 104579098 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 5 variations for ASPG
         3 CNVs: 49289 29961 29960
         2 Indels: 87378 76745

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ASPG for disorders           About GeneDecksing

    3 diseases for ASPG:    About MalaCards
    nasopharynx carcinoma    cystadenoma    carcinoma


    Export disorders for ASPG gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ASPG gene integrated from 9 sources:
    (articles sorted by number of sources associating them with ASPG)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Structures of apo and product-bound human L-asparaginase: insights into the mechanism of autoproteolysis and substrate hydrolysis. (PubMed id 22861376)1 Nomme J.... Lavie A. (2012)
    2. 60kDa lysophospholipase, a new Sgk1 molecular partner involved in the regulation of ENaC. (PubMed id 21063096)1 Menniti M....Perrotti N. (2010)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
    4. The DNA sequence and analysis of human chromosome 14. (PubMed id 12508121)2 Heilig R.... Weissenbach J. (2003)
    5. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 374569 HGNC: 20123 AceView: LOC374569 Ensembl:ENSG00000166183 euGenes: HUgn374569
    ECgene: ASPG H-InvDB: ASPG

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for ASPG Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for ASPG gene:
    Search GeneIP for patents involving ASPG

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
    About This Section

     
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    GeneCards Homepage - Last full update: 18 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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    von Willebrand factor
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