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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

ASNS Gene

protein-coding GIFtS: 64
GCID: GC07M097481

asparagine synthetase (glutamine-hydrolyzing)

(Previous name: asparagine synthetase )

Explore 21 diseases affiliated with
ASNS via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Asparagine Synthetase (Glutamine-Hydrolyzing)¹ ²		Asparagine Synthetase¹
Glutamine-Dependent Asparagine Synthetase² ³		Asparagine Synthetase [Glutamine-Hydrolyzing]²
TS11² ³		TS11 Cell Cycle Control Protein²
EC 6.3.5.4³ ⁸		Cell Cycle Control Protein TS11³

External Ids:	HGNC: 753¹	Entrez Gene: 440²	Ensembl: ENSG00000070669⁷	OMIM: 108370⁵	UniProtKB: P08243³

Export aliases for ASNS gene to outside databases

Previous GC identifers: GC07M096016 GC07M097079 GC07M097093 GC07M097126 GC07M097319 GC07M092082

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ASNS:

The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the

temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at

nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. (provided by

RefSeq, May 2010)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000007.13 NC_018918.1 NT_007933.15 NT_079595.2

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the ASNS gene promoter:
AML1a
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): ASNS promoter sequence

Search SABiosciences Chromatin IP Primers for ASNS

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ASNS

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7q21.3 Ensembl cytogenetic band: 7q21.3 HGNC cytogenetic band: 7q21.3

ASNS Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ASNS gene location

GeneLoc information about chromosome 7 GeneLoc Exon Structure

GeneLoc location for GC07M097481: view genomic region (about GC identifiers)

Start:	97,481,429 bp from pter	End:	97,501,854 bp from pter
Size:	20,426 bases	Orientation:	minus strand

1 alternative location:

Chr7-,CRA_TCAG 96,803,932-96,824,039

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ASNS_HUMAN, P08243 (See protein sequence)

Recommended Name: Asparagine synthetase [glutamine-hydrolyzing]

Size: 561 amino acids; 64370 Da

Secondary accessions: A4D1I8 B4DXZ1 B7ZAA9 D6W5R3 E9PCI3 E9PCX6 P08184 Q15666 Q549T9 Q96HD0

Alternative splicing: 3 isoforms: P08243-1 P08243-2 P08243-3 (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for ASNS: NX_P08243

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_P08243

4/18 DME Specific Peptides for ASNS (P08243) (see all 18)

FEFEYQT

SGEGSDE

SYYLSLP

NGEIYNH

ASNS Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (6 alternative transcripts):

NP_001171546.1 NP_001171547.1 NP_001171548.1 NP_001664.3 NP_597680.2 NP_899199.2

ENSEMBL proteins:

ENSP00000377846 ENSP00000401651 ENSP00000414379 ENSP00000377845 ENSP00000414901

ENSP00000408472 ENSP00000406994 ENSP00000400422 ENSP00000394242 ENSP00000402350

ENSP00000408797 ENSP00000397802 ENSP00000413797 ENSP00000175506

Reactome Protein details: P08243
Human Recombinant Protein Products for ASNS:

	Browse Purified and Recombinant Proteins at EMD Millipore
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	OriGene Purified Proteins (see all 3): ASNS OriGene Protein Over-expression Lysate (see all 4): ASNS OriGene Custom Protein Services for ASNS
	GenScript Custom Purified and Recombinant Proteins Services for ASNS
	Novus Biologicals ASNS Proteins Novus Biologicals ASNS Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for ASNS

Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005625	soluble fraction	--	--
GO:0005829	cytosol	TAS	--

ASNS for ontologies About GeneDecksing

ASNS Antibody Products:

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	Uscn Antibodies for ASNS
	Search ThermoFisher Antibodies for ASNS

Assay Products for ASNS:

	Browse Kits and Assays available from EMD Millipore
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	GenScript Custom Assay Services for ASNS
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for ASNS

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

ASNS for domains About GeneDecksing

5 InterPro domains/families:

IPR000583 GATase_dom

IPR006426 Asn_synth_AEB

IPR014729 Rossmann-like_a/b/a_fold

IPR001962 Asn_synthase

IPR017932 GATase_2_dom

Graphical View of Domain Structure for InterPro Entry P08243

ProtoNet protein and cluster: P08243

1 Blocks protein family: IPB001962 Asparagine synthase

UniProtKB/Swiss-Prot: ASNS_HUMAN, P08243

Similarity: Contains 1 asparagine synthetase domain

Similarity: Contains 1 glutamine amidotransferase type-2 domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: ASNS_HUMAN, P08243

Catalytic activity: ATP + L-aspartate + L-glutamine + H(2)O = AMP + diphosphate + L-asparagine + L-glutamate

Genatlas biochemistry entry for ASNS:

asparagine synthetase

Enzyme Number (IUBMB): EC 6.3.5.4¹ ²

Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004066	asparagine synthase (glutamine-hydrolyzing) activity	IDA	2573597
GO:0005524	ATP binding	IEA	--
GO:0042803	protein homodimerization activity	IEA	--
GO:0048037	cofactor binding	IEA	--

ASNS for ontologies About GeneDecksing

Phenotypes:
1 GenomeRNAi human phenotype for ASNS:

Decreased Salmonella enterica

7 MGI mutant phenotypes (inferred from 1 allele) (MGI details for Asns):

cardiovascular system	growth/size	hematopoietic system	homeostasis/metabolism	immune system
pigmentation	vision/eye

ASNS for phenotypes About GeneDecksing

Animal Models:

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for ASNS

miRNA Products:		OriGene 3'-UTR Clone (see all 3): ASNS Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat ASNS
		Search QIAGEN for miScript miRNA Assays for microRNAs that regulate ASNS
		Browse SwitchGear 3'UTR luciferase reporter plasmids

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for ASNS (see all 7) OriGene shRNA RFP: ASNS OriGene siRNA: ASNS
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat ASNS
		Sirion Biotech Custom design and validation of potent shRNA sequences against ASNS

Gene Editing Products:			DNA2.0 Custom Protein Engineering Service for ASNS
			Sirion Biotech Customized adenovirus for overexpression of ASNS Sirion Biotech Customized adenovirus for potent knockdown of ASNS

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ASNS (see all 8) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ASNS (see all 6) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 6): ASNS (NM_183356)
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		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ASNS

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for ASNS
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In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ASNS

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/10 super-pathways (see all 10) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

glutamine degradation I

	glutamine degradation I	1.00			asparagine biosynthesis I	0.33
	Expression of Asparagine Synthetase	0.33

Metabolism

Metabolism

1.00

Metabolic pathways

0.38

superpathway of citrulline metabolism

superpathway of citrulline metabolism

1.00

citrulline biosynthesis

0.53

Activation of Genes by ATF4

Activation of Genes by ATF4

1.00

PERK regulated gene expression

0.64

Amino acid synthesis and interconversion (transamination)

Amino acid synthesis and interconversion (transamination)

1.00

Pathway sources
See GeneCards unified pathways
Show all pathways

4 BioSystems Pathways for ASNS

	citrulline biosynthesis
	superpathway of citrulline metabolism
	asparagine biosynthesis I
	glutamine degradation I

5/8 Reactome Pathways for ASNS (see all 8)

	Metabolism
	Amino acid synthesis and interconversion (transamination)
	Expression of Asparagine Synthetase
	PERK regulated gene expression
	Metabolism of proteins

3 Kegg Pathways (Kegg details for ASNS):

	Alanine, aspartate and glutamate metabolism
	Nitrogen metabolism
	Metabolic pathways

UniProtKB/Swiss-Prot: ASNS_HUMAN, P08243

Pathway: Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1

ASNS for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for ASNS

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/60 Interacting proteins for ASNS (P08243^{2, 3} ENSP00000175506⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 60)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
STRN4	Q9NRL3²^,³	MINT-65600 I2D: score=5
CTPS1	P17812³, ENSP00000361699⁴	I2D: score=2 STRING: ENSP00000361699
PWP1	Q13610³, ENSP00000258531⁴	I2D: score=2 STRING: ENSP00000258531
UBC	P0CG48³, ENSP00000344818⁴	I2D: score=2 STRING: ENSP00000344818
IKBKE	Q14164³, ENSP00000356087⁴	I2D: score=1 STRING: ENSP00000356087

About this table

Gene Ontology (GO): 5/21 biological process terms (GO ID links to tree view) (see all 21): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0001889	liver development	IEA	--
GO:0006520	cellular amino acid metabolic process	--	--
GO:0006529	asparagine biosynthetic process	IDA	2573597
GO:0006541	glutamine metabolic process	IEA	--
GO:0006987	activation of signaling protein activity involved in unfolded protein response	TAS	--

ASNS for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

ASNS for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for ASNS

10 HMDB Compounds for ASNS About this table

Compound	Synonyms	CAS #	PubMed Ids
Adenosine monophosphate	5'-AMP (see all 28)	61-19-8	--
Adenosine triphosphate	5'-(tetrahydrogen triphosphate) Adenosine (see all 24)	56-65-5	--
Famotidine	Amfamox (see all 83)	76824-35-6	--
L-Asparagine	(-)-Asparagine (see all 32)	70-47-3	--
L-Aspartic acid	(+)-Aspartate (see all 41)	56-84-8	--
L-Glutamic acid	(2S)-2-Aminopentanedioate (see all 49)	56-86-0	--
L-Glutamine	(2S)-2,5-diamino-5-oxopentanoic acid (see all 31)	56-85-9	--
Phosphoric acid	acide phosphorique (FRENCH) (see all 20)	7664-38-2	--
Pyrophosphate	(4-)Diphosphoric acid ion (see all 10)	14000-31-8	--
Water	Dihydrogen oxide (see all 2)	7732-18-5	--

5 DrugBank Compounds for ASNS About this table

Compound	Synonyms	CAS #	Type	Actions	PubMed Ids
L-Asparagine	(-)-Asparagine (see all 15)	70-47-3	target	--	14990305 17380207 12706338 15574844 16164412
L-Glutamine	(S)-2,5-diamino-5-oxopentanoic acid (see all 4)	56-85-9	enzyme	substrate	16931516 16164412 17329242 16870293 17397190
Adenosine triphosphate	5'-ATP (see all 8)	56-65-5	target	--	17139284 17016423
L-Aspartic Acid	(+)-Aspartic acid (see all 23)	56-84-8	target	--	17139284 17016423
L-Glutamic Acid	(2S)-2-Aminopentanedioic acid (see all 15)	56-86-0	target	--	17139284 17016423

10/13 Novoseek chemical compound relationships for ASNS gene (see all 13) About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
asparagine	94.6	187	16164412 (5), 1359839 (4), 16923354 (4), 1358303 (4) (see all 97)
albizziin	90.3	3	8105542 (2)
amino acid	69.8	11	11677247 (1), 12628003 (1), 10856289 (1), 18840095 (1) (see all 6)
glutamine	57.4	10	1359839 (3), 8102140 (3), 12805577 (1), 11551215 (1) (see all 6)
carbamoyl phosphate	43.5	2	11395405 (1), 12859215 (1)
aspartate	37.9	3	16164412 (1), 8102140 (1), 11551215 (1)
amide	30.4	2	8102140 (1), 12890666 (1)
glutamate	18.6	3	15385533 (1), 12859215 (1)
glucose	14.3	5	11677247 (1), 12628003 (1), 12881527 (1), 17409444 (1)
histidine	1.06	1	10948265 (1)

Search CenterWatch for drugs/clinical trials and news about ASNS

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for ASNS gene (6 alternative transcripts):

NM_001178075.1 NM_001178076.1 NM_001178077.1 NM_001673.4 NM_133436.3 NM_183356.3

Unigene Cluster for ASNS:

Asparagine synthetase (glutamine-hydrolyzing)

Hs.489207 [show with all ESTs]

Unigene Representative Sequence: NM_183356

17 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000394309(uc003uot.4 uc011kin.2) ENST00000454046 ENST00000437628

ENST00000394308 ENST00000422745 ENST00000455086 ENST00000444334 ENST00000487714

ENST00000462436 ENST00000442734 ENST00000495255 ENST00000437657 ENST00000448127

ENST00000453600 ENST00000451771 ENST00000414884 ENST00000175506(uc011kio.2 uc003uou.4 uc003uov.4 uc003uox.4)

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		Browse SwitchGear 3'UTR luciferase reporter plasmids

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for ASNS (see all 7) OriGene shRNA RFP: ASNS OriGene siRNA: ASNS
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		Sirion Biotech Custom design and validation of potent shRNA sequences against ASNS

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ASNS (see all 8) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ASNS (see all 6) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 6): ASNS (NM_183356)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for ASNS
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ASNS

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for ASNS Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat ASNS
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ASNS
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ASNS

Additional cDNA sequence:

AK000379.1 AK302189.1 AK302242.1 AK316224.1 BC008723.2 BC014621.2 BT007113.1 M15798.1

M27396.1

24/27 DOTS entries (see all 27):

DT.92457837 DT.452523 DT.100877448 DT.100877477 DT.99982693 DT.121050523 DT.100839795 DT.92457840

DT.97821209 DT.121050297 DT.99948035 DT.100030257 DT.95262520 DT.75125009 DT.95262541 DT.95262517

DT.101981674 DT.91894956 DT.100877476 DT.100877480 DT.100877482 DT.101958426 DT.121050382 DT.436578

24/637 AceView cDNA sequences (see all 637):

AA424926 BU178677 NM_183356 CR607694 BE271424 BM790241 AU125889 BQ448630

CA446777 AK125166 AL559639 BU675019 BX384188 CB134584 AI090292 AA894927

AI632334 BG118510 BU169447 BT007113 BI254975 BF797368 BE882357 BQ005112

GeneLoc Exon Structure

5/9 Alternative Splicing Database (ASD) splice patterns (SP) for ASNS (see all 9) About this scheme

ExUns:	1a	·	1b	·	1c	·	1d	^	2	^	3	^	4a	·	4b	·	4c	^	5a	·	5b	·	5c	^	6	^	7	^	8a	·	8b	^	9	^	10a	·	10b	^	11a	·	11b	^	12	^	13	^	14a	·	14b
SP1:																																					-		-
SP2:									-		-		-
SP3:									-		-		-		-		-
SP4:
SP5:									-		-

ECgene alternative splicing isoforms for ASNS

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

ASNS expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GTCTTGAACT

About this image

ASNS expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

6 LifeMap In Vivo Development Anatomical Compartments/Cells

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Eye

Inner Nuclear Layer

Mature Horizontal Cells

Horizontal, Retina

Neural Ectoderm

Brain

Eye

Neural Tube

Spinal Cord

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

Stem Cell Differentiation: 2 LifeMap Cells

Name

Category

PureStem™ mesenchymal progenitor 4D20.8 (Embryonic Progenitor Cell)

Lateral Plate Mesoderm, Neural Crest

PureStem™ mesenchymal progenitor E15 (Embryonic Progenitor Cell)

Adipose, Bone, Cartilage

See ASNS Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for ASNS

SOURCE GeneReport for Unigene cluster: Hs.489207

SABiosciences Expression via Pathway-Focused PCR Arrays including ASNS:

Amino Acid Metabolism II in human mouse rat

Molecular Toxicology PathwayFinder 384HT in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for ASNS Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat ASNS
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ASNS
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ASNS

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ASNS

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the last universal common ancestor (LUCA).

Orthologs for ASNS gene from 10/31 species (see all 31) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Asns^{1 , 5}	asparagine synthetase^{1, 5}	87.76(n)¹ 93.94(a)¹		6 (3.31 cM)⁵ 27053¹ NM_012055.3¹ NP_036185.1¹ 7675169⁵
chicken (Gallus gallus)	Aves	ASNS¹	asparagine synthetase (glutamine-hydrolyzing)	77.02(n) 80.71(a)		420574 NM_001030977.1 NP_001026148.1
lizard (Anolis carolinensis)	Reptilia	ASNS⁶	--	80(a)	1 ↔ 1	6(23533921-23545920)
tropical clawed frog (Xenopus tropicalis)	Amphibia	Str.4783²	Transcribed sequence with moderate similarity to protein more	74.96(n)		BQ390084.1
zebrafish (Danio rerio)	Actinopterygii	zgc56605²	similar to asparagine synthetase	75.24(n)		394138 BC052127.1
fruit fly (Drosophila melanogaster)	Insecta	asparagine-synthetase⁶	asparagine synthetase	36(a)	1 ↔ 1	3L(21166218-21168073)
worm (Caenorhabditis elegans)	Secernentea	nrs-2³ asns-1¹	asparagine synthetase³ Protein ASNS-1¹	36(a)³ 50.92(n)¹ 40.45(a)¹		V(8548883-8551113)³ 184935¹ NM_072803.2¹ NP_505204.1¹
thale cress (Arabidopsis thaliana)	eudicotyledons	ASN2⁶ ASN3⁶ (see all 3)	asparagine synthetase 3 (see all 3)	38(a) 37(a) (see all 3)	possible ortholog possible ortholog (see all 3)	5(25969189-25972575) 5(3212688-3216466)
rice (Oryza sativa)	Liliopsida	Os.135²	Oryza sativa (japonica cultivar-group) mRNA for asparagine more	75.45(n)		AK100247.1
E. coli (Escherichia coli)	Gamma proteobacteria	asnB⁶	asparagine synthetase B	36(a)	1 ↔ 1	Chromosome(696736-698400)

ENSEMBL Gene Tree for ASNS (if available)
TreeFam Gene Tree for ASNS (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for ASNS gene

ASNS for paralogs About GeneDecksing

5/13 Pseudogenes.org Pseudogenes for ASNS (see all 13)
PGOHUM00000248482 PGOHUM00000234995 PGOHUM00000244189 PGOHUM00000244888 PGOHUM00000244900

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 7 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs7803055^1,2	C,F	--	97480989(+)	`TAAAAT/CATTTA`	6	--	ds500¹		5		NA EA	246
rs76666194^1,2		--	97480997(+)	`TTACTA/CTCTGG`	6	--	ds500¹		0	--	--	--	--
rs192526067^1,2		--	97481016(+)	`TAAAAA/GCAAAT`	6	--	ds500¹		0	--	--	--	--
rs114630405^1,2	F	--	97481195(+)	`GGCAGA/CACAAA`	6	--	ds500¹		1		WA	118
rs183104287^1,2		--	97481223(+)	`ATGACA/GTCCCT`	6	--	ds500¹		0	--	--	--	--
rs75618284^1,2	F	--	97481228(+)	`GTCCCT/AAAGAT`	6	--	ds500¹		2		WA EA	238
rs35190368^1,2	C,F	--	97481248(+)	`ACTGGA/TAGGAA`	6	--	ds500¹		7		NA CSA WA EA	368
rs187441560^1,2		--	97481261(+)	`CACACA/GGGCAC`	6	--	ds500¹		0	--	--	--	--
rs75198492^1,2	C,F	--	97481466(+)	`TTTTTC/AACACC`	6	--	ut3¹		1		EA	120
rs115270247^1,2	F	--	97481491(+)	`TTGACT/GCTCAT`	6	--	ut3¹		1		WA	118

HapMap Linkage Disequilibrium report for ASNS (97481429 - 97501854 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for ASNS
1 CNV: 3691
Human Gene Mutation Database (HGMD): ASNS

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ASNS

DNA2.0 Custom Variant and Variant Library Synthesis for ASNS

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

ASNS for disorders           About GeneDecksing

OMIM gene information: 108370    OMIM disorders: --

20/21 diseases for ASNS (see all 21):    About MalaCards

non-hodgkin lymphoma lymphoblastic leukemia hodgkin's lymphoma acute lymphoblastic leukemia

acute lymphocytic leukemia lymphocytic leukemia bipolar disorder leukemia

ovarian cancer hepatocellular carcinoma pancreatic cancer breast cancer

pancreatitis sarcoma hypoxia schizophrenia

tuberculosis carcinoma malaria prostatitis

8 Novoseek disease relationships for ASNS gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
lymphoblastic leukemia acute childhood	64.5	5	16923354 (2), 15718422 (1), 16497975 (1), 19035175 (1)
lymphoblastic leukemia acute	62.9	4	18541474 (2), 16023613 (1), 19035175 (1)
t-all	58.7	4	16923354 (4)
leukemia	58.5	13	17185229 (2), 11485552 (2), 1976872 (2), 18541474 (1) (see all 10)
ovarian cancer	29.8	6	17088436 (2), 18852115 (1), 19209300 (1), 16648555 (1)
tumors	0	3	12894236 (1), 12802930 (1), 8093451 (1)
lymphoma	0	1	16156856 (1)
cancer	0	1	17088436 (1)

Human Genome Epidemiology (HuGE) Navigator: ASNS (1 document)

Export disorders for ASNS gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for ASNS gene, integrated from 9 sources (see all 145):
(articles sorted by number of sources associating them with ASNS)

Utopia: connect your pdf to the dynamic
world of online information

Mesenchymal cells regulate the response of acute lymphoblastic leukemia cells to asparaginase. (PubMed id 17380207)^{1, 7} Iwamoto S....Campana D. (2007)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
The DNA sequence of human chromosome 7. (PubMed id 12853948)^{1, 2} Hillier L.W.... Wilson R.K. (2003)
Human chromosome 7: DNA sequence and biology. (PubMed id 12690205)^{1, 2} Scherer S.W.... Tsui L.-C. (2003)
Organization and expression of the cell cycle gene, ts11, that encodes asparagine synthetase. (PubMed id 2569668)^{1, 2} Greco A.... Basilico C. (1989)
Expression of human asparagine synthetase in Escherichia coli. (PubMed id 2564390)^{1, 2} van Heeke G. and Schuster S.M. (1989)
Molecular structure of the human asparagine synthetase gene. (PubMed id 2565875)^{1, 2} Zhang Y.P....Andrulis I.L. (1989)
The N-terminal cysteine of human asparagine synthetase is essential for glutamine-dependent activity. (PubMed id 2573597)^{1, 2} van Heeke G. and Schuster S.M. (1989)
Isolation of human cDNAs for asparagine synthetase and expression in Jensen rat sarcoma cells. (PubMed id 2886907)^{1, 2} Andrulis I.L.... Ray P.N. (1987)
Molecular cloning of a gene that is necessary for G1 progression in mammalian cells. (PubMed id 3470743)^{1, 2} Greco A.... Basilico C. (1987)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
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(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 440	HGNC: 753	AceView: ASNS	Ensembl:ENSG00000070669	euGenes: HUgn440
ECgene: ASNS	Kegg: 440	H-InvDB: ASNS

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for ASNS	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for ASNS	Genetics and Cytogenetics in Oncology and Haematology

Intellectual Property for ASNS gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for ASNS gene:
Search GeneIP for patents involving ASNS

GeneCards and IP:
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asparagine synthetase (glutamine-hydrolyzing)

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