Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



ASAP2 Gene

protein-coding   GIFtS: 60
GCID: GC02P009346

ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 2

(Previous name: development and differentiation enhancing factor 2)
(Previous symbol: DDEF2)
  See ASAP2-related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
ArfGAP With SH3 Domain, Ankyrin Repeat And PH Domain 21 2     centaurin1
DDEF21 2 3 5     AMAP22
PAP2 3 5     CENTB32
Development And Differentiation Enhancing Factor 21 2     Pap-alpha2
Development And Differentiation-Enhancing Factor 22 3     SHAG12
Paxillin-Associated Protein With ARF GAP Activity 32 3     Arf-GAP With SH3 Domain, ANK Repeat And PH Domain-Containing Protein 22
Pyk2 C-Terminus-Associated Protein2 3     Centaurin, Beta 32
PAG32 3     PYK2 C Terminus-Associated Protein2
Beta 31     KIAA04003

External Ids:    HGNC: 27211   Entrez Gene: 88532   Ensembl: ENSG000001516937   OMIM: 6038175   UniProtKB: O431503   

Export aliases for ASAP2 gene to outside databases

Previous GC identifers: GC02P009264 GC02P009193


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for ASAP2 Gene:
This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif,
followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich
region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the
plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a
stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the
C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has
catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP
activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding
when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and
SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants
encoding different isoforms have been found for this gene. (provided by RefSeq, Sep 2008)

GeneCards Summary for ASAP2 Gene:
ASAP2 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 2) is a protein-coding gene. Diseases associated with ASAP2 include cri-du-chat syndrome. GO annotations related to this gene include phospholipid binding and ARF GTPase activator activity. An important paralog of this gene is AGAP5.

UniProtKB/Swiss-Prot: ASAP2_HUMAN, O43150
Function: Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and
modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN
recruitment to focal contacts and cell migration

Gene Wiki entry for ASAP2 (DDEF2) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000002.11  NT_005334.17  NC_018913.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the ASAP2 gene promoter:
         RFX1   FOXD3   CUTL1   STAT5A   POU3F2   NF-kappaB   GATA-1   POU2F1   POU2F1a   NF-kappaB1   
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search Chromatin IP Primers for ASAP2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ASAP2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2p25|2p24   Ensembl cytogenetic band:  2p25.1   HGNC cytogenetic band: 2p24

ASAP2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ASAP2 gene location

GeneLoc information about chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P009346:  view genomic region     (about GC identifiers)

Start:
9,346,894 bp from pter      End:
9,545,812 bp from pter
Size:
198,919 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: ASAP2_HUMAN, O43150 (See protein sequence)
Recommended Name: Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2  
Size: 1006 amino acids; 111651 Da
Subunit: Binds PXN, ARF1, ARF5, ARF6, PTK2B and SRC
Sequence caution: Sequence=BAA23696.2; Type=Erroneous initiation;
Secondary accessions: D6W4Y8
Alternative splicing: 2 isoforms:  O43150-1   O43150-2   

Explore the universe of human proteins at neXtProt for ASAP2: NX_O43150

Explore proteomics data for ASAP2 at MOPED

Post-translational modifications: 

  • Phosphorylated on tyrosine residues by SRC and PTK2B1
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See ASAP2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001128663.1  NP_003878.1  

    ENSEMBL proteins: 
     ENSP00000281419   ENSP00000316404  

    ASAP2 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for ASAP2
    OriGene Custom MassSpec
    OriGene Custom Protein Services for ASAP2
    GenScript Custom Purified and Recombinant Proteins Services for ASAP2
    Novus Biologicals ASAP2 Proteins
    Novus Biologicals ASAP2 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

     
    Search eBioscience for Proteins for ASAP2 

    ASAP2 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    Browse OriGene Antibodies
    OriGene Custom Antibody Services for ASAP2
    Novus Biologicals ASAP2 Antibodies
    Search for Antibodies for ASAP2 at Abcam
    Browse Antibodies at Cloud-Clone Corp.
    ThermoFisher Antibodies for ASAP2
    LSBio Antibodies in human, mouse, rat for ASAP2

    ASAP2 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for ASAP2
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for ASAP2
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.
    Search eBioscience for ELISAs for ASAP2 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    HGNC Gene Families:
    PLEKH: Pleckstrin homology (PH) domain containing
    ANKRD: Ankyrin repeat domain containing
    ArfGAP: ADP-ribosylation factor GTPase activating proteins

    Selected InterPro protein domains (see all 7):
     IPR027267 AH/BAR-dom
     IPR001849 Pleckstrin_homology
     IPR001164 ArfGAP
     IPR001452 SH3_domain
     IPR011993 PH_like_dom

    Graphical View of Domain Structure for InterPro Entry O43150

    ProtoNet protein and cluster: O43150

    4 Blocks protein domains:
    IPB001164 HIV Rev interacting protein signature
    IPB001452 SH3 domain signature
    IPB001849 Pleckstrin-like
    IPB002110 Ankyrin repeat signature


    UniProtKB/Swiss-Prot: ASAP2_HUMAN, O43150
    Domain: The conserved Arg-464 in the Arf-GAP domain probably becomes part of the active site of bound small
    GTPases and is necessary for GTP hydrolysis
    Similarity: Contains 2 ANK repeats
    Similarity: Contains 1 Arf-GAP domain
    Similarity: Contains 1 PH domain
    Similarity: Contains 1 SH3 domain


    Find genes that share domains with ASAP2           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ASAP2_HUMAN, O43150
    Function: Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and
    modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN
    recruitment to focal contacts and cell migration
    Induction: Up-regulated during monocytic maturation

         Gene Ontology (GO): 4 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI11716503
    GO:0008047enzyme activator activity TAS9819391
    GO:0008060ARF GTPase activator activity IEA--
    GO:0008270zinc ion binding IEA--
         
    Find genes that share ontologies with ASAP2           About GenesLikeMe


    Phenotypes:
         1 GenomeRNAi human phenotype for ASAP2:
     Synthetic lethal with c-Myc af 

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ASAP2
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ASAP2

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for ASAP2
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for ASAP2

    miRNA
    Products:
        
    miRTarBase miRNAs that target ASAP2:
    hsa-mir-375 (MIRT019707)

    Block miRNA regulation of human, mouse, rat ASAP2 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate ASAP2 (see all 48):
    hsa-miR-520f hsa-miR-300 hsa-miR-4328 hsa-miR-548k hsa-miR-199a-3p hsa-miR-485-3p hsa-miR-29a hsa-miR-371-5p
    SwitchGear 3'UTR luciferase reporter plasmidASAP2 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for ASAP2
    Predesigned siRNA for gene silencing in human, mouse, rat ASAP2

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for ASAP2

    Clone
    Products:
         
    OriGene clones in human, mouse for ASAP2 (see all 12)
    OriGene ORF clones in mouse, rat for ASAP2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): ASAP2 (NM_001135191)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ASAP2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ASAP2

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for ASAP2
    Browse ESI BIO Cell Lines and PureStem Progenitors for ASAP2 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ASAP2


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    ASAP2_HUMAN, O43150: Cytoplasm. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Cell
    membrane; Peripheral membrane protein. Note=Colocalizes with F-actin and ARF6 in phagocytic cups
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    golgi apparatus5
    plasma membrane5
    cytosol2
    nucleus2

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886plasma membrane IEA--
    GO:0032580Golgi cisterna membrane IEA--

    Find genes that share ontologies with ASAP2           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for ASAP2 About    
    See pathways by source

    SuperPathContained pathways About
    1Fc gamma R-mediated phagocytosis
    Fc gamma R-mediated phagocytosis
    2Endocytosis
    Endocytosis
    3Arf6 trafficking events
    Arf6 trafficking events


    Find genes that share SuperPaths with ASAP2           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 BioSystems Pathway for ASAP2
        Arf6 trafficking events


    2 Kegg Pathways  (Kegg details for ASAP2):
        Endocytosis
    Fc gamma R-mediated phagocytosis

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for ASAP2
    Interactions:

        GeneGlobe Interaction Network for ASAP2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for ASAP2 (O431501, 2, 3 ENSP000002814194) via UniProtKB, MINT, STRING, and/or I2D (see all 45)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PLCG1P191741, 2, 3, ENSP000002440074EBI-310968,EBI-79387 MINT-8112435 I2D: score=3 STRING: ENSP00000244007
    PIK3R1P279862, 3, ENSP000002743354MINT-8111811 I2D: score=3 STRING: ENSP00000274335
    SH3KBP1Q96B972, 3, ENSP000003809214MINT-4300058 I2D: score=3 STRING: ENSP00000380921
    CBLBQ131912, 3, ENSP000002641224MINT-4300041 I2D: score=1 STRING: ENSP00000264122
    UBCP0CG481, 2, 3, ENSP000003448184EBI-310968,EBI-3390054 MINT-4299964 MINT-4299995 I2D: score=1 STRING: ENSP00000344818
    About this table

    Gene Ontology (GO): 2 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0032312regulation of ARF GTPase activity IEA--
    GO:0043085positive regulation of catalytic activity TAS9819391

    Find genes that share ontologies with ASAP2           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ASAP2

    1 HMDB Compound for ASAP2    About this table
    CompoundSynonyms CAS #PubMed Ids
    Guanosine triphosphate5'-GTP (see all 10)86-01-1--



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for ASAP2 gene (2 alternative transcripts): 
    NM_001135191.1  NM_003887.2  

    Unigene Cluster for ASAP2:

    ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
    Hs.555902  [show with all ESTs]
    Unigene Representative Sequence: NM_003887
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000281419(uc002qzh.2) ENST00000471687 ENST00000491413 ENST00000484590
    ENST00000315273(uc002qzi.2)
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat ASAP2 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate ASAP2 (see all 48):
    hsa-miR-520f hsa-miR-300 hsa-miR-4328 hsa-miR-548k hsa-miR-199a-3p hsa-miR-485-3p hsa-miR-29a hsa-miR-371-5p
    SwitchGear 3'UTR luciferase reporter plasmidASAP2 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for ASAP2
    Predesigned siRNA for gene silencing in human, mouse, rat ASAP2
    Clone
    Products:
         
    OriGene clones in human, mouse for ASAP2 (see all 12)
    OriGene ORF clones in mouse, rat for ASAP2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): ASAP2 (NM_001135191)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ASAP2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ASAP2
    Primer
    Products:
        
    OriGene qSTAR qPCR primer pairs in human, mouse for ASAP2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ASAP2
      QuantiTect SYBR Green Assays in human, mouse, rat ASAP2
      QuantiFast Probe-based Assays in human, mouse, rat ASAP2

    Additional mRNA sequence: 

    AB007860.1 AK091121.1 AK123185.1 AK124900.1 BC063308.1 

    12 DOTS entries:

    DT.216312  DT.87007471  DT.100774013  DT.40213623  DT.91728245  DT.100774010  DT.92059657  DT.100774015 
    DT.121022109  DT.121022199  DT.70101858  DT.95206773 

    Selected AceView cDNA sequences (see all 186):

    F11743 T06154 BQ880084 F06551 BM980536 N62808 BU628454 BQ002724 
    BG925617 Z39513 BQ423127 F04059 AI143427 AI708030 BG120690 CB160529 
    BM676090 CD673250 BM978959 AW627522 AA343776 AA709068 F04352 BQ009344 

    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for ASAP2    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13a · 13b ^ 14a · 14b ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20 ^ 21 ^ 22 ^ 23 ^
    SP1:                                                                                -                 -                                                         
    SP2:                                                                                -                 -                                                     -   
    SP3:                                                                                                                                                            
    SP4:                                                                                                                                                            

    ExUns: 24 ^ 25a · 25b ^ 26 ^ 27 ^ 28
    SP1:        -                           
    SP2:        -                           
    SP3:                                    
    SP4:                                    


    ECgene alternative splicing isoforms for ASAP2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    ASAP2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    ASAP2 Expression
    About this image


    ASAP2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 4) fully expand
     
     Lung (Respiratory System)
             Basal Cells Trachea
     
     Limb (Muscoskeletal System)
             Prechondrocytic Mesenchymal Cells Zeugopod
     
     Bone (Muscoskeletal System)
             Prechondrocytic Mesenchymal Cells Zeugopod
     
     Cartilage (Muscoskeletal System)
             Prechondrocytic Mesenchymal Cells Zeugopod
    ASAP2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ASAP2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.555902

    UniProtKB/Swiss-Prot: ASAP2_HUMAN, O43150
    Tissue specificity: Detected in heart, brain, placenta, kidney, monocytes and pancreas

        Custom PCR Arrays for ASAP2
    Primer
    Products:
    OriGene qSTAR qPCR primer pairs in human, mouse for ASAP2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ASAP2
    QuantiTect SYBR Green Assays in human, mouse, rat ASAP2
    QuantiFast Probe-based Assays in human, mouse, rat ASAP2
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ASAP2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for ASAP2 gene from Selected species (see all 16)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Asap21 , 5 ArfGAP with SH3 domain, ankyrin repeat and PH domain more1, 5 87.5(n)1
    95.09(a)1
      12 (8.29 cM)5
    2119141  NM_001135192.11  NP_001128664.11 
     211117485 
    chicken
    (Gallus gallus)
    Aves ASAP21 ArfGAP with SH3 domain, ankyrin repeat and PH domain more 78.6(n)
    89.51(a)
      768599  XM_001231631.3  XP_001231632.3 
    lizard
    (Anolis carolinensis)
    Reptilia ASAP26
    ArfGAP with SH3 domain, ankyrin repeat and PH doma...
    85(a)
    1 ↔ 1
    1(148510641-148655460)
    African clawed frog
    (Xenopus laevis)
    Amphibia BG554458.12   -- 73.93(n)    BG554458.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.239322 Transcribed sequence with moderate similarity to protein more 72.17(n)    CD760178.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Asap16
    ArfGAP with SH3 domain, ankyrin repeat and PH doma...
    38(a)
    1 → many
    2R(4001791-4010357)


    ENSEMBL Gene Tree for ASAP2 (if available)
    TreeFam Gene Tree for ASAP2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for ASAP2 gene
    AGAP52  AGAP62  ASAP12  ARAP32  ACAP22  ACAP32  ARAP12  AGAP32  
    ARAP22  AGAP22  AGAP42  AGAP12  AGAP82  ACAP12  AGAP72  AGAP102  
    AGAP92  ASAP32  
    4 SIMAP similar genes for ASAP2 using alignment to 1 protein entry:     ASAP2_HUMAN:
    DDEF2    ASAP1    ASAP3    ARFGAP1

    Find genes that share paralogs with ASAP2           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for ASAP2 (see all 4365)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 2 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1136001351,2
    C,F--9344991(+) GTAGGC/AAAGTT 2 -- us2k11Minor allele frequency- A:0.50CSA 2
    rs598623281,2
    C,F--9349129(+) ATACCC/-TTTCT 2 -- int13Minor allele frequency- -:0.33NA CSA 6
    rs1925571731,2
    --9349203(+) TTACCC/GTAGAG 2 -- int10--------
    rs1845345531,2
    --9349283(+) CTGCCA/GTCTAA 2 -- int10--------
    rs1869048401,2
    --9349365(+) TCTACA/TTACAT 2 -- int10--------
    rs1913652981,2
    --9349385(+) ATGGTA/CTGTGT 2 -- int10--------
    rs1850794501,2
    --9349431(+) ACTTTC/GTGAGG 2 -- int10--------
    rs1439310721,2
    --9349444(+) AATCAA/GTGTGG 2 -- int10--------
    rs1467627421,2
    C--9349528(+) TTAATA/CACAAT 2 -- int10--------
    rs1894585001,2
    C--9349631(+) AAATAA/CATAAA 2 -- int10--------

    HapMap Linkage Disequilibrium report for ASAP2 (9346894 - 9545812 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 7 variations for ASAP2:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2478125CNV Deletion19546169
    esv2662547CNV Deletion23128226
    nsv2601CNV Insertion18451855
    esv2604725CNV Insertion19546169
    esv272973CNV Insertion20981092
    esv25422CNV Loss19812545
    esv29614CNV Gain19812545

    Human Gene Mutation Database (HGMD): ASAP2
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing ASAP2
    DNA2.0 Custom Variant and Variant Library Synthesis for ASAP2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 603817    OMIM disorders: --

    1 disease for ASAP2:    
    About MalaCards
    cri-du-chat syndrome

    1 disease from the University of Copenhagen DISEASES database for ASAP2:
    Cri-Du-Chat syndrome

    Find genes that share disorders with ASAP2           About GenesLikeMe

    Genetic Association Database (GAD): ASAP2
    Human Genome Epidemiology (HuGE) Navigator: ASAP2 (1 document)

    Export disorders for ASAP2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for ASAP2 gene, integrated from 10 sources (see all 32):
    (articles sorted by number of sources associating them with ASAP2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification of a new Pyk2 target protein with Arf-GAP activity. (PubMed id 10022920)1, 2, 3, 9 Andreev J.... Schlessinger J. (Mol. Cell. Biol. 1999)
    2. Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 9455477)1, 2, 3 Ishikawa K.... Ohara O. (DNA Res. 1997)
    3. A new paxillin-binding protein, PAG3/Papalpha/KIAA0400, bearing an ADP-ribosylation factor GTPase-activating protein activity, is involved in paxillin recruitment to focal adhesions and cell migration. (PubMed id 10749932)1, 2, 9 Kondo A.... Sabe H. (Mol. Biol. Cell 2000)
    4. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)1, 2 Olsen J.V....Mann M. (Cell 2006)
    5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    6. PAG3/Papalpha/KIAA0400, a GTPase-activating protein for ADP- ribosylation factor (ARF), regulates ARF6 in Fcgamma receptor-mediated phagocytosis of macrophages. (PubMed id 11304556)1, 2 Uchida H.... Sabe H. (J. Exp. Med. 2001)
    7. A novel mode of action of an ArfGAP, AMAP2/PAG3/Papa lpha, in Arf6 function. (PubMed id 15231847)1, 9 Hashimoto S....Sabe H. (J. Biol. Chem. 2004)
    8. The ASAP2 gene is a primary target of 1,25-dihydroxyvitamin D3 in human monocytes and macrophages. (PubMed id 23999061)1 Seuter S....Carlberg C. (J. Steroid Biochem. Mol. Biol. 2013)
    9. Autophagy negatively regulates cancer cell proliferation via selectively targeting VPRBP. (PubMed id 22963397)1 Wang B.S....Wang M.R. (Clin. Sci. 2013)
    10. Integrative analysis of the ubiquitin proteome isolated using Tandem Ubiquitin Binding Entities (TUBEs). (PubMed id 22178446)1 Lopitz-Otsoa F....Rodriguez M.S. (J Proteomics 2012)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 8853 HGNC: 2721 AceView: DDEF2 Ensembl:ENSG00000151693 euGenes: HUgn8853
    ECgene: ASAP2 Kegg: 8853 H-InvDB: ASAP2

    (According to HUGE)
    About This Section

    TryGeneCards Plus
    HUGE: KIAA0400

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for ASAP2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for ASAP2 gene:
    Search GeneIP for patents involving ASAP2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     
     EMD Millipore genomic analysis products

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     Browse OriGene Antibodies   OriGene RNAi products in human, mouse, rat for ASAP2  
     Browse OriGene qPCR primer pairs and template standards   OriGene Protein Over-expression Lysate for ASAP2  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for ASAP2  
     OriGene qSTAR qPCR primer pairs in human, mouse for ASAP2   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for ASAP2   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for ASAP2   OriGene Custom Protein Services for ASAP2  

     
     
     Block miRNA regulation of human, mouse, rat ASAP2 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing ASAP2
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ASAP2 Predesigned siRNA for gene silencing in human, mouse, rat ASAP2
     QuantiFast Probe-based Assays in human, mouse, rat ASAP2 QuantiTect SYBR Green Assays in human, mouse, rat ASAP2
     Custom PCR Arrays for ASAP2 Search Chromatin IP Primers for ASAP2
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ASAP2  GeneGlobe Interaction Network for ASAP2
     Regulatory tfbs in ASAP2 promoter
     GenScript Custom Purified and Recombinant Proteins Services for ASAP2 GenScript cDNA clones with any tag delivered in your preferred vector for ASAP2
     GenScript Custom Assay Services for ASAP2 GenScript Custom overexpressing Cell Line Services for ASAP2
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Search Tocris compounds for ASAP2
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     ASAP2 antibodies
     ASAP2 proteins
     ASAP2 lysates
     Search for Antibodies for ASAP2 at Abcam
     See all of Abcam's Antibodies, Kits and Proteins for ASAP2
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Browse Proteins at Cloud-Clone Corp.
     Browse Antibodies at Cloud-Clone Corp.
     Browse ELISAs at Cloud-Clone Corp.
     Browse CLIAs at Cloud-Clone Corp.



     Browse ESI BIO Cell Lines and PureStem Progenitors for ASAP2
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ASAP2
     SwitchGear 3'UTR luciferase reporter plasmids for ASAP2
     Browse SwitchGear Promoter luciferase reporter plasmids for ASAP2
     ThermoFisher Antibodies for ASAP2
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ASAP2
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ASAP2
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ASAP2
     LSBio Antibodies in human, mouse, rat for ASAP2
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
     Search Addgene for plasmids for ASAP2
      Search eBioscience for proteins for ASAP2
      Search eBioscience for elisas for ASAP2
      eBioscience FlowRNA Probe Sets
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      ASAP2 gene at Home site.
    Version: 3.12.224 13 Oct 2014
    hostname: 356977-web1.xennexinc.com index build: 128 solr: 1.4