Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 
or upload a file of gene symbols


Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ARHGEF4 Gene

protein-coding   GIFtS: 64
GCID: GC02P131595

Rho Guanine Nucleotide Exchange Factor (GEF) 4

  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
Rho Guanine Nucleotide Exchange Factor (GEF) 41 2     STM62
APC-Stimulated Guanine Nucleotide Exchange Factor 12 3     Rho Guanine Nucleotide Exchange Factor 42
ASEF2 5     Asef3
APC-Stimulated Guanine Nucleotide Exchange Factor1     Asef13
ASEF12     KIAA11123
GEF42     

External Ids:    HGNC: 6841   Entrez Gene: 506492   Ensembl: ENSG000001360027   OMIM: 6052165   UniProtKB: Q9NR803   

Export aliases for ARHGEF4 gene to outside databases

Previous GC identifers: GC02P129362 GC02P129196 GC02P131696 GC02P131884 GC02P131507 GC02P131390 GC02P123836


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ARHGEF4 Gene:
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli
that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins
and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different
isoforms have been found, but the full-length nature of some variants has not been determined. (provided by
RefSeq, Jun 2013)

GeneCards Summary for ARHGEF4 Gene: 
ARHGEF4 (Rho guanine nucleotide exchange factor (GEF) 4) is a protein-coding gene. Diseases associated with ARHGEF4 include familial adenomatous polyposis, and adenoma, and among its related super-pathways are Regulation of actin cytoskeleton and NRAGE signals death through JNK. GO annotations related to this gene include phospholipid binding and guanyl-nucleotide exchange factor activity. An important paralog of this gene is ARHGEF6.

UniProtKB/Swiss-Prot: ARHG4_HUMAN, Q9NR80
Function: Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may
activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in
E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in
colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and
tumor progression

Gene Wiki entry for ARHGEF4 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000002.11  NT_022135.16  NC_018913.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ARHGEF4 gene promoter:
         NF-1   NF-1/L   AP-1   NF-kappaB   HNF-4alpha1   E47   MZF-1   PPAR-gamma1   PPAR-gamma2   NF-kappaB1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidARHGEF4 promoter sequence
   Search SABiosciences Chromatin IP Primers for ARHGEF4

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ARHGEF4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2q22   Ensembl cytogenetic band:  2q21.1   HGNC cytogenetic band: 2q22

ARHGEF4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ARHGEF4 gene location

GeneLoc information about chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P131595:  view genomic region     (about GC identifiers)

Start:
131,594,489 bp from pter      End:
131,804,836 bp from pter
Size:
210,348 bases      Orientation:
plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: ARHG4_HUMAN, Q9NR80 (See protein sequence)
Recommended Name: Rho guanine nucleotide exchange factor 4  
Size: 690 amino acids; 79067 Da
Subunit: Isoform 3 interacts with RHOA and RAC1, and (via ABR domain) with APC. Found in a complex consisting of
ARHGEF4, APC and CTNNB1
Subcellular location: Isoform 3: Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein;
Cytoplasmic side (Probable). Note=Associated with membrane ruffles (Probable)
Sequence caution: Sequence=AAF79955.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
Sequence=AAF79955.1; Type=Frameshift; Positions=638, 643; Sequence=BAA83064.1; Type=Erroneous initiation;
Note=Translation N-terminally shortened;
4 PDB 3D structures from and Proteopedia for ARHGEF4:
2DX1 (3D)        2PZ1 (3D)        3NMX (3D)        3NMZ (3D)    
Secondary accessions: Q9HDC6 Q9UPP0
Alternative splicing: 3 isoforms:  Q9NR80-1   Q9NR80-3   Q9NR80-4   

Explore the universe of human proteins at neXtProt for ARHGEF4: NX_Q9NR80

Explore proteomics data for ARHGEF4 at MOPED 

Post-translational modifications:

  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_Q9NR80

  • ARHGEF4 Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    ARHGEF4 Protein Expression
    REFSEQ proteins (2 alternative transcripts): 
    NP_056135.2  NP_127462.1  

    ENSEMBL proteins: 
     ENSP00000386794   ENSP00000316845   ENSP00000389661   ENSP00000432267   ENSP00000387285  
     ENSP00000348017   ENSP00000432301   ENSP00000398455   ENSP00000376680  
    Reactome Protein details: Q9NR80
    Human Recombinant Protein Products for ARHGEF4: 
    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for ARHGEF4
    OriGene Custom MassSpec 
    OriGene Custom Protein Services for ARHGEF4
    GenScript Custom Purified and Recombinant Proteins Services for ARHGEF4
    Novus Biologicals ARHGEF4 Proteins
    Novus Biologicals ARHGEF4 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates 
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for ARHGEF4 

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005829cytosol TAS--
    GO:0032587ruffle membrane IEA--

    ARHGEF4 for ontologies           About GeneDecksing



    ARHGEF4 Antibody Products: 
    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    OriGene Antibodies for ARHGEF4
    OriGene Custom Antibody Services for ARHGEF4
    GenScript Custom Superior Antibodies Services for ARHGEF4
    Novus Biologicals ARHGEF4 Antibodies
    Abcam antibodies for ARHGEF4
    Cloud-Clone Corp. Antibodies for ARHGEF4 
    ThermoFisher Antibodies for ARHGEF4
    LSBio Antibodies in human, mouse, rat for ARHGEF4 

    Assay Products for ARHGEF4: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for ARHGEF4
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for ARHGEF4
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for ARHGEF4 
    Cloud-Clone Corp. CLIAs for ARHGEF4


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    HGNC Gene Families: 
    PLEKH: Pleckstrin homology (PH) domain containing
    ARHGEF: Rho guanine nucleotide exchange factors

    5 InterPro protein domains:
     IPR001331 GDS_CDC24_CS
     IPR000219 DH-domain
     IPR001849 Pleckstrin_homology
     IPR001452 SH3_domain
     IPR011993 PH_like_dom

    Graphical View of Domain Structure for InterPro Entry Q9NR80

    ProtoNet protein and cluster: Q9NR80

    3 Blocks protein domains:
    IPB000219 DH domain
    IPB001452 SH3 domain signature
    IPB001849 Pleckstrin-like


    UniProtKB/Swiss-Prot: ARHG4_HUMAN, Q9NR80
    Similarity: Contains 1 DH (DBL-homology) domain
    Similarity: Contains 1 PH domain
    Similarity: Contains 1 SH3 domain


    ARHGEF4 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ARHG4_HUMAN, Q9NR80
    Function: Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may
    activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in
    E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in
    colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and
    tumor progression

         Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005085guanyl-nucleotide exchange factor activity IMP17599059
    GO:0005089Rho guanyl-nucleotide exchange factor activity NAS--
    GO:0005515protein binding IPI17588722
    GO:0005543phospholipid binding IEA--
    GO:0019904protein domain specific binding IPI10947987
         
    ARHGEF4 for ontologies           About GeneDecksing


    Phenotypes:
         5 GenomeRNAi human phenotypes for ARHGEF4:
     Decreased G3BP1 protein expres  Decreased focal adhesion (FA)   Decreased viability  Elongated cells 
     Upregulation of Wnt/beta-caten 

         11 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Arhgef4):
     adipose tissue  behavior/neurological  cardiovascular system  cellular  hematopoietic system 
     homeostasis/metabolism  immune system  integument  other  tumorigenesis 
     vision/eye 

    ARHGEF4 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Arhgef4tm1Taki for ARHGEF4

       inGenious Targeting Laboratory - Custom generated mouse model solutions for ARHGEF4 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for ARHGEF4

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for ARHGEF4 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for ARHGEF4 

    miRNA
    Products:
        
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat ARHGEF4
    8/24 QIAGEN miScript miRNA Assays for microRNAs that regulate ARHGEF4 (see all 24):
    hsa-miR-3910 hsa-miR-136 hsa-miR-301a hsa-miR-124 hsa-miR-629* hsa-miR-149 hsa-miR-506 hsa-miR-130b
    SwitchGear 3'UTR luciferase reporter plasmidARHGEF4 3' UTR sequence
    Inhib. RNA
    Products:
        
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for ARHGEF4
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat ARHGEF4

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for ARHGEF4
    Sirion Biotech Customized adenovirus for overexpression of ARHGEF4

    Clone
    Products:
         
    Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene clones in human, mouse for ARHGEF4 (see all 11)
    OriGene ORF clones in mouse, rat for ARHGEF4
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): ARHGEF4 (NM_015320)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ARHGEF4
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ARHGEF4
    Sirion Biotech Customized lentivirus for stable overexpression of ARHGEF4 
                         Customized lentivirus expression plasmids for stable overexpression of ARHGEF4 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for ARHGEF4
    Search LifeMap BioReagents cell lines for ARHGEF4
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ARHGEF4


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for ARHGEF4 About   (see all 21)                                                                                              See pathways by source

    SuperPathContained pathways About
    1Regulation of Actin Cytoskeleton
    Regulation of Actin Cytoskeleton0.57
    Regulation of actin cytoskeleton0.57
    2Cell death signalling via NRAGE, NRIF and NADE
    Cell death signalling via NRAGE, NRIF and NADE0.74
    p75 NTR receptor-mediated signalling0.73
    NRAGE signals death through JNK0.74
    G alpha (12/13) signalling events0.46
    3GPCR Pathway
    Ras Pathway0.73
    Pancreatic Adenocarcinoma0.59
    GPCR Pathway0.62
    Breast Cancer Regulation by Stathmin10.58
    4Signaling by GPCR
    Signaling by GPCR0.90
    Signal Transduction0.55
    GPCR downstream signaling0.90
    5G-AlphaQ Signaling
    G-AlphaQ Signaling0.47
    Thrombin Signaling0.40
    G-AlphaI Signaling0.47

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 EMD Millipore Pathway for ARHGEF4
        G-protein signaling Regulation of RAC1 activity

    5/21 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for ARHGEF4 (see all 21)
        RhoA Pathway
    Guidance Cues and Growth Cone Motility
    Molecular Mechanisms of Cancer
    Interferon Pathway
    G-AlphaI Signaling

    1 GeneGo (Thomson Reuters) Pathway for ARHGEF4
        G-protein signaling Regulation of RAC1 activity

    2 BioSystems Pathways for ARHGEF4
        Regulation of Actin Cytoskeleton
    Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)

    5/10        Reactome Pathways for ARHGEF4 (see all 10)
        GPCR downstream signaling
    Signaling by Rho GTPases
    G alpha (12/13) signalling events
    Cell death signalling via NRAGE, NRIF and NADE
    Signaling by GPCR


    1         Kegg Pathway  (Kegg details for ARHGEF4):
        Regulation of actin cytoskeleton


    ARHGEF4 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for ARHGEF4

    STRING Interaction Network Preview (showing 5 interactants - click image to see 10)

    5/84 Interacting proteins for ARHGEF4 (Q9NR802, 3 ENSP000003168454) via UniProtKB, MINT, STRING, and/or I2D (see all 84)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HLA-BP018893, ENSP000003991684I2D: score=3 STRING: ENSP00000399168
    APCP250543, ENSP000002574304I2D: score=2 STRING: ENSP00000257430
    RHOAP615863, ENSP000004001754I2D: score=2 STRING: ENSP00000400175
    RAC1P630003, ENSP000003484614I2D: score=3 STRING: ENSP00000348461
    NEDD8Q158432, ENSP000002504954MINT-6542674 STRING: ENSP00000250495
    About this table

    Gene Ontology (GO): 5/9 biological process terms (GO ID links to tree view) (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007264small GTPase mediated signal transduction TAS--
    GO:0030032lamellipodium assembly IMP17599059
    GO:0035023regulation of Rho protein signal transduction IEA--
    GO:0035556intracellular signal transduction NAS--
    GO:0043065positive regulation of apoptotic process TAS--

    ARHGEF4 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ARHGEF4 (ARHG4)

    3 HMDB Compounds for ARHGEF4    About this table
    CompoundSynonyms CAS #PubMed Ids
    Guanosine diphosphate5'-GDP (see all 10)146-91-8--
    Guanosine monophosphate5'-GMP (see all 14)85-32-5--
    Guanosine triphosphate5'-GTP (see all 10)86-01-1--

    Search CenterWatch for drugs/clinical trials and news about ARHGEF4 / ARHG4

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ARHGEF4 gene (2 alternative transcripts): 
    NM_015320.3  NM_032995.2  

    Unigene Cluster for ARHGEF4:

    Rho guanine nucleotide exchange factor (GEF) 4
    Hs.469935  [show with all ESTs]
    Unigene Representative Sequence: AK127124
    16 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000526381 ENST00000409359(uc002try.1 uc002trz.1 uc010fmw.1)
    ENST00000326016 ENST00000438985 ENST00000525839 ENST00000409303 ENST00000355771
    ENST00000496764 ENST00000439368 ENST00000528247 ENST00000490728(uc002tsc.1)
    ENST00000532720 ENST00000527365 ENST00000525092 ENST00000428230 ENST00000392953(uc002tsa.1 uc002tsb.1 uc010fmx.1)

    miRNA
    Products:
         
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat ARHGEF4
    8/24 QIAGEN miScript miRNA Assays for microRNAs that regulate ARHGEF4 (see all 24):
    hsa-miR-3910 hsa-miR-136 hsa-miR-301a hsa-miR-124 hsa-miR-629* hsa-miR-149 hsa-miR-506 hsa-miR-130b
    SwitchGear 3'UTR luciferase reporter plasmidARHGEF4 3' UTR sequence
    Inhib. RNA
    Products:
         
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for ARHGEF4
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat ARHGEF4
    Clone
    Products:
         
    OriGene clones in human, mouse for ARHGEF4 (see all 11)
    OriGene ORF clones in mouse, rat for ARHGEF4
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): ARHGEF4 (NM_015320)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ARHGEF4
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ARHGEF4
    Sirion Biotech Customized lentivirus for stable overexpression of ARHGEF4 
                         Customized lentivirus expression plasmids for stable overexpression of ARHGEF4 
    Primer
    Products:
        
    OriGene qSTAR qPCR primer pairs in human, mouse for ARHGEF4
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat ARHGEF4
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ARHGEF4
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ARHGEF4

    Additional mRNA sequence: 

    AB029035.1 AB042199.1 AF249745.1 AK127124.1 AL137289.1 BC036579.1 BC139742.1 DQ424905.1 

    8 DOTS entries:

    DT.100736562  DT.452051  DT.97765665  DT.213810  DT.91843041  DT.92027570  DT.40297726  DT.91808163 

    24/86 AceView cDNA sequences (see all 86):

    AA636021 T08962 F04706 BM710167 AL137289 CD673186 BF056255 T33534 
    AI696820 AI677831 AB042199 Z40248 BM671590 BU845201 BM998905 AB029035 
    BM924000 AK127124 T15412 AI634119 BQ689151 R38781 AW167377 NM_032995 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for ARHGEF4    About this scheme

    ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17a · 17b
    SP1:                                -           -           -                                                         
    SP2:                                -           -           -                                                         
    SP3:                                            -           -                                                         
    SP4:                                                        -                                                         
    SP5:                                                                                                                  


    ECgene alternative splicing isoforms for ARHGEF4

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ARHGEF4 expression in normal human tissues (normalized intensities)      ARHGEF4 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GACAGTCACT
    ARHGEF4 Expression
    About this image


    ARHGEF4 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database 
     5/2 selected tissues (see all 2) fully expand
     
     Brain (Nervous System)    fully expand to see all 3 entries
             caudate nucleus   
     
     Gut Tube (Gastrointestinal Tract)
             Definitive endoderm-like cells ( A scalable, suspension protocol for derivation of...

    See ARHGEF4 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ARHGEF4

    SOURCE GeneReport for Unigene cluster: Hs.469935

    UniProtKB/Swiss-Prot: ARHG4_HUMAN, Q9NR80
    Tissue specificity: Expressed at high levels in the brain, skeletal muscle and testis and at low levels in the
    kidney, lung, small intestine, ovary and prostate. Expression is aberrantly enhanced in most colorectal tumors

        SABiosciences Custom PCR Arrays for ARHGEF4
    Primer
    Products:
    OriGene qSTAR qPCR primer pairs in human, mouse for ARHGEF4
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat ARHGEF4
    QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ARHGEF4
    QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ARHGEF4
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ARHGEF4

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for ARHGEF4 gene from 7/15 species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Arhgef41 , 5 Rho guanine nucleotide exchange factor (GEF) 41, 5 88.41(n)1
    93.37(a)1
      1 (13.34 cM)5
    2269701  NM_183019.21  NP_898840.21 
     346781885 
    chicken
    (Gallus gallus)
    Aves ARHGEF41 Rho guanine nucleotide exchange factor (GEF) 4 77.14(n)
    80.33(a)
      424761  XM_422582.3  XP_422582.2 
    lizard
    (Anolis carolinensis)
    Reptilia ARHGEF46
    Rho guanine nucleotide exchange factor (GEF) 4
    75(a)
    1 ↔ 1
    3(37520341-37741634)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.136032 Transcribed sequence with moderate similarity to protein more 74.55(n)    BX732986.1 
    zebrafish
    (Danio rerio)
    Actinopterygii arhgef41 Rho guanine nucleotide exchange factor (GEF) 4 74.05(n)
    76.04(a)
      559017  XM_682312.4  XP_687404.2 
    fruit fly
    (Drosophila melanogaster)
    Insecta GEFmeso6
    Guanine nucleotide exchange factor in mesoderm
    6(a)
    1 → many
    2R(14441624-14499260)
    worm
    (Caenorhabditis elegans)
    Secernentea uig-16
    Protein UIG-1, isoform d
    9(a)
    1 → many
    V(13273567-13295913)


    ENSEMBL Gene Tree for ARHGEF4 (if available)
    TreeFam Gene Tree for ARHGEF4 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ARHGEF4 gene
    ARHGEF62  VAV12  PLEKHG22  PLEKHG32  PREX22  ARHGEF72  TIAM12  SPATA132  
    TIAM22  ARHGEF92  VAV32  VAV22  PREX12  PLEKHG12  
    6 SIMAP similar genes for ARHGEF4 using alignment to 8 protein entries:     ARHG4_HUMAN (see all proteins):
    DKFZp434G2016    SPATA13    ARHGEF9    ARHGEF7    PREX2    SH3GL2

    ARHGEF4 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/2683 SNPs in ARHGEF4 are shown (see all 2683)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 2 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0359714
    A breast cancer sample4--see VAR_0359712 T R mis40--------
    VAR_0359704
    A breast cancer sample4--see VAR_0359702 K R mis40--------
    rs1807414481,2
    --131672228(+) GTGAAA/CTGGGT 2 -- int10--------
    rs776380881,2
    --131672231(+) AACTGC/GGTCGG 2 -- int10--------
    rs67527241,2
    C,H--131672271(+) GGCACC/TGGTGC 2 -- int114Minor allele frequency- T:0.00NA WA CSA EA 377
    rs579470881,2
    C,F--131672292(+) AGGCCC/TACTGG 2 -- int11Minor allele frequency- T:0.03EA 120
    rs1166508371,2
    C,F--131672388(+) CCACTC/TGGATG 2 -- int11Minor allele frequency- T:0.01NA 120
    rs739603721,2
    C,F--131672630(+) ATGAAC/TGAGAT 2 -- int11Minor allele frequency- T:0.50WA 2
    rs794062691,2
    F--131672670(+) ACCCAC/TCCAGC 2 -- int11Minor allele frequency- T:0.02EA 120
    rs124641191,2
    C,F,H--131672950(+) ACCCAG/ACTCGC 2 -- int112Minor allele frequency- A:0.40NA WA CSA EA EU 953

    HapMap Linkage Disequilibrium report for ARHGEF4 (131594489 - 131804836 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 10/13 variations for ARHGEF4 (see all 13):    About this table     
    Variant IDTypeSubtypePubMed ID
    esv2670824CNV Deletion23128226
    dgv659e201CNV Deletion23290073
    dgv731e199CNV Deletion23128226
    esv1708111CNV Insertion17803354
    nsv428404CNV Loss18775914
    nsv875104CNV Loss21882294
    nsv524021CNV Loss19592680
    essv11772CNV CNV17122850
    dgv1247e1CNV Complex17122850
    dgv1253e1CNV Complex17122850


    Human Gene Mutation Database (HGMD): ARHGEF4
    SABiosciences Cancer Mutation PCR Assays
    SeqTarget long-range PCR primers for resequencing ARHGEF4
    DNA2.0 Custom Variant and Variant Library Synthesis for ARHGEF4

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    OMIM gene information: 605216    OMIM disorders: --

    6 diseases for ARHGEF4:    About MalaCards
    familial adenomatous polyposis    adenoma    adenocarcinoma    pancreatitis
    breast cancer    prostatitis


    ARHGEF4 for disorders           About GeneDecksing


    Export disorders for ARHGEF4 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ARHGEF4 gene, integrated from 9 sources (see all 25):
    (articles sorted by number of sources associating them with ARHGEF4)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Isolation of two novel human RhoGEFs, ARHGEF3 and ARHGEF4, in 3p13-21 and 2q22. (PubMed id 10873612)1, 2, 3, 9 Thiesen S.... Nothwang H.G. (2000)
    2. Identification and characterization of Asef2, a guanine-nucleotide exchange factor specific for Rac1 and Cdc42. (PubMed id 17599059)1, 2 Kawasaki Y....Akiyama T. (2007)
    3. Mutated APC and Asef are involved in the migration of colorectal tumour cells. (PubMed id 12598901)1, 2 Kawasaki Y.... Akiyama T. (2003)
    4. Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. (PubMed id 12168954)1, 2 Nakajima D.... Nagase T. (2002)
    5. Asef, a link between the tumor suppressor APC and G-protein signaling. (PubMed id 10947987)1, 2 Kawasaki Y.... Akiyama T. (2000)
    6. Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10470851)1, 2 Kikuno R....Ohara O. (1999)
    7. Comprehensive analysis of interactions between the Src -associated protein in mitosis of 68 kDa and the human Src-homology 3 proteome. (PubMed id 22745667)1 Asbach B....Wagner R. (2012)
    8. Small rare recurrent deletions and reciprocal duplicat ions in 2q21.1, including brain-specific ARHGEF4 and GPR148. (PubMed id 22543972)1 Dharmadhikari A.V....Stankiewicz P. (2012)
    9. Structural basis for the recognition of Asef by adenom atous polyposis coli. (PubMed id 21788986)1 Zhang Z....Wu G. (2012)
    10. A human MAP kinase interactome. (PubMed id 20936779)1 Bandyopadhyay S....Ideker T. (2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 50649 HGNC: 684 AceView: ARHGEF4 Ensembl:ENSG00000136002 euGenes: HUgn50649
    ECgene: ARHGEF4 Kegg: 50649 H-InvDB: ARHGEF4

    (According to HUGE)
    About This Section
    HUGE: KIAA1112

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for ARHGEF4 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for ARHGEF4 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for ARHGEF4 gene:
    Search GeneIP for patents involving ARHGEF4

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

     
     EMD Millipore Custom Antibody & Bulk Services
     EMD Millipore Preclinical / Clinical Development Services
     EMD Millipore Immunoassay Services
     EMD Millipore Target Screening & Profiling Services

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Kinase Activity Assays/Reagents  
     Browse ELISpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Multiplex/Array Assay Kits/Reagents  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Protease Activity Assays and Reagents   Browse Recombinant/Natural Proteins  
     Browse Stem Cell Products   Browse Tocris Biochemicals & Compounds  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for ARHGEF4   OriGene RNAi products in human, mouse, rat for ARHGEF4  
     Browse OriGene qPCR primer pairs and template standards   OriGene Protein Over-expression Lysate for ARHGEF4  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for ARHGEF4  
     OriGene qSTAR qPCR primer pairs in human, mouse for ARHGEF4   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for ARHGEF4   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for ARHGEF4   OriGene Custom Protein Services for ARHGEF4  

     
     
     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat ARHGEF4
     QIAGEN SeqTarget long-range PCR primers for resequencing ARHGEF4
     QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ARHGEF4
     QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat ARHGEF4
     QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ARHGEF4
     QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ARHGEF4
     GenScript Custom Purified and Recombinant Proteins Services for ARHGEF4 GenScript cDNA clones with any tag delivered in your preferred vector for ARHGEF4
     GenScript Custom Assay Services for ARHGEF4 GenScript Custom Superior Antibodies Services for ARHGEF4
     GenScript Custom overexpressing Cell Line Services for ARHGEF4 CloneReady with Over 120,000 Genes
     Gene Synthesis: Any Gene in Any Vector Vector-based siRNA and miRNA, Ready for Transfection
     Gene Mutant Library, Variants up to 10^11 Plasmid Preparation
     Custom Peptide Services
     Search for Antibodies & Assays

     Regulatory tfbs in ARHGEF4 promoter
     Search Chromatin IP Primers for ARHGEF4
     RT2 qPCR Primer Assay in human, mouse, rat ARHGEF4
     GNC Network for ARHGEF4
     SABiosciences PCR Arrays including human, mouse, rat ARHGEF4
     Search Tocris compounds for ARHGEF4 (ARHG4)
     Browse Sino Biological Proteins and Antibodies
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
     Novus Tissue Slides
     ARHGEF4 antibodies
     ARHGEF4 proteins
     ARHGEF4 lysates
     Antibodies for ARHGEF4
     See all of Abcam's Antibodies, Kits and Proteins for ARHGEF4
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins




     Proteins for ARHGEF4
     Antibodies for ARHGEF4
     ELISAs for ARHGEF4
     CLIAs for ARHGEF4
     Search LifeMap BioReagents cell lines for ARHGEF4
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ARHGEF4
     SwitchGear 3'UTR luciferase reporter plasmids for ARHGEF4
     SwitchGear Promoter luciferase reporter plasmids for ARHGEF4
     ThermoFisher Antibodies for ARHGEF4
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ARHGEF4
     inGenious Targeting Laboratory - Custom generated mouse model solutions for ARHGEF4
     inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for ARHGEF4
    Customized:
     lentivirus for stable overexpression of ARHGEF4
     lentivirus expression plasmids for stable overexpression of ARHGEF4
     adenovirus for overexpression of ARHGEF4
     LSBio Antibodies in human, mouse, rat for ARHGEF4
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
           
    GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 4 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

    View Random Gene

    Category
    (GIFtS: 73)
    transforming growth factor, beta 1
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      ARHGEF4 gene at Home site.
    hostname: 356980-web2.xennexinc.com index build: 106 solr: 1.4