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ARHGEF35 Gene

protein-coding   GIFtS: 30
GCID: GC07M143883

Rho Guanine Nucleotide Exchange Factor (GEF) 35

(Previous name: Rho guanine nucleotide exchange factor (GEF) 5-like)
(Previous symbol: ARHGEF5L)
  Search for ARHGEF35
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Rho Guanine Nucleotide Exchange Factor (GEF) 351 2
ARHGEF5L1 2 3
Rho Guanine Nucleotide Exchange Factor 5-Like Protein2 3
Rho Guanine Nucleotide Exchange Factor (GEF) 5-Like1
Rho Guanine Nucleotide Exchange Factor 352

External Ids:    HGNC: 338461   Entrez Gene: 4453282   Ensembl: ENSG000002132147   UniProtKB: A5YM693   

Export aliases for ARHGEF35 gene to outside databases

Previous GC identifer: GC07M138082


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for ARHGEF35 Gene:
ARHGEF35 (Rho guanine nucleotide exchange factor (GEF) 35) is a protein-coding gene.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000007.14  NC_018918.2  NT_007933.16  
Regulatory elements:
   Search for regulatory transcription factor binding sites for ARHGEF35
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search Chromatin IP Primers for ARHGEF35

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ARHGEF35


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7q35   Ensembl cytogenetic band:  7q35   HGNC cytogenetic band: 7q35

ARHGEF35 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ARHGEF35 gene location

GeneLoc information about chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07M143883:  view genomic region     (about GC identifiers)

Start:
143,883,176 bp from pter      End:
143,991,230 bp from pter
Size:
108,055 bases      Orientation:
minus strand

1 alternative location:
Chr7-,CRA_TCAG 143,221,434-143,231,049     

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: ARG35_HUMAN, A5YM69 (See protein sequence)
Recommended Name: Rho guanine nucleotide exchange factor 35  
Size: 484 amino acids; 53287 Da
Secondary accessions: Q6ZUI2

Explore the universe of human proteins at neXtProt for ARHGEF35: NX_A5YM69

Explore proteomics data for ARHGEF35 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See ARHGEF35 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_001003702.2  
    ENSEMBL proteins: 
     ENSP00000367355   ENSP00000438502  

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    ARHGEF: Rho guanine nucleotide exchange factors

    ProtoNet protein and cluster: A5YM69


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Animal Models:
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus3
    cytosol2

    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for ARHGEF35
    Interactions:

        Search GeneGlobe Interaction Network for ARHGEF35

    STRING Interaction Network Preview (showing 2 interactants - click image to see more details)

    2 Interacting proteins for ARHGEF35 (ENSP000003673554) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ELAVL1ENSP000003852694STRING: ENSP00000385269
    UBCENSP000003448184STRING: ENSP00000344818
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ARHGEF35 (ARG35)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for ARHGEF35 gene: 
    NM_001003702.2  

    Unigene Cluster for ARHGEF35:

    Rho guanine nucleotide exchange factor (GEF) 35
    Hs.534621  [show with all ESTs]
    Unigene Representative Sequence: NM_001003702
    2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000378115(uc003wdz.2) ENST00000543357
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    Additional mRNA sequence: 

    AK125680.1 BC136868.1 BC136878.1 DQ786206.1 EF560743.1 

    5 DOTS entries:

    DT.91743124  DT.97806985  DT.95088786  DT.121037396  DT.100649037 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    ARHGEF35 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    ARHGEF35 Expression
    About this image

    ARHGEF35 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ARHGEF35 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.534621
        Custom PCR Arrays for ARHGEF35
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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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      --

    ENSEMBL Gene Tree for ARHGEF35 (if available)
    TreeFam Gene Tree for ARHGEF35 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for ARHGEF35 gene
    2 SIMAP similar genes for ARHGEF35 using alignment to 1 protein entry:     ARG35_HUMAN:
    ARHGEF5    DKFZp686N1969

    ARHGEF35 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for ARHGEF35 (see all 86)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 7 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs2008953651,2
    C--143883762(+) GCTGAC/TGAAAC 1 -- ut310--------
    rs2004052441,2
    C--143884022(+) ACATAG/TCAAAG 2 * C --0--------
    rs1136544341,2
    C--143884028(+) CAAAGT/AACTGA 2 /V syn11Minor allele frequency- A:0.50CSA 2
    rs2011206341,2
    C--143884034(+) ACTGAT/GAGAAG 2 /L syn11Minor allele frequency- G:0.00EU 859
    rs47266761,2
    C,F,H--143884103(+) TGAGAG/ACATCT 2 /C syn18Minor allele frequency- A:0.09NS EA NA 4534
    rs17313481,2
    C,F--143884106(+) GAGCAA/C/TCTGAT 3 R S syn1 mis12MN EU 1298
    rs29513131,2
    C,F--143884122(+) CTAGCA/GAGGGA 2 S L mis11Minor allele frequency- G:0.00EU 1261
    rs2014643551,2
    C--143884139(+) GCTGCA/GGGGGA 2 P syn10--------
    rs1442760541,2
    C,F--143884150(+) CAGTTC/TCTCAG 2 K E mis11Minor allele frequency- T:0.00NA 3734
    rs2007779661,2
    C,F--143884216(+) AAACAG/CGTCAC 2 /L /V mis11Minor allele frequency- C:0.00EU 869

    HapMap Linkage Disequilibrium report for ARHGEF35 (143883176 - 143991230 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for ARHGEF35 (see all 28):    About this table    
    Variant IDTypeSubtypePubMed ID
    dgv24n31CNV Duplication19718026
    nsv471454CNV Duplication19718026
    nsv821609CNV Duplication20802225
    nsv819701CNV Insertion19587683
    nsv831174CNV Loss17160897
    nsv831175CNV Loss17160897
    dgv7525n71CNV Loss21882294
    nsv831176CNV Loss17160897
    nsv482116CNV Gain20164927
    esv33253CNV Gain17666407

    Site Specific Mutation Identification with PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for ARHGEF35 gene integrated from 10 sources:
    (articles sorted by number of sources associating them with ARHGEF35)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Systematic and quantitative assessment of the ubiquitin-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (Mol. Cell 2011)
    2. Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (EMBO J. 2009)
    3. A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)2 Dephoure N.... Gygi S.P. (Proc. Natl. Acad. Sci. U.S.A. 2008)
    4. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (Genome Res. 2006)
    5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    6. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (Nat. Genet. 2004)
    7. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
    8. (PubMed id )2 

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 445328 HGNC: 33846 Ensembl:ENSG00000213214 euGenes: HUgn445328 ECgene: ARHGEF35
    H-InvDB: ARHGEF35

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for ARHGEF35 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for ARHGEF35 gene:
    Search GeneIP for patents involving ARHGEF35

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ARHGEF35
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