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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ARHGEF26 Gene

protein-coding   GIFtS: 46
GCID: GC03P153839

Rho guanine nucleotide exchange factor (GEF) 26

 Explore 6 diseases affiliated with
ARHGEF26 via our new
 Human Malady Compendium 
Biological research products
for ARHGEF26
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Rho Guanine Nucleotide Exchange Factor (GEF) 261 2     CSGEF2
SGEF1 2 3     HMFN18642
SH3 Domain-Containing Guanine Exchange Factor2 3     Rho Guanine Nucleotide Exchange Factor 262
DKFZP434D1461     Src Homology 3 Domain-Containing Guanine Nucleotide Exchange Factor2

External Ids:    HGNC: 244901   Entrez Gene: 260842   Ensembl: ENSG000001147907   UniProtKB: Q96DR73   

Export aliases for ARHGEF26 gene to outside databases

Previous GC identifer: GC03P151230


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ARHGEF26:
This gene encodes a member of the Rho-guanine nucleotide exchange factor (Rho-GEF) family. These proteins regulate Rho
GTPases by catalyzing the exchange of GDP for GTP. The encoded protein specifically activates RhoG and plays a role in
the promotion of macropinocytosis. Underexpression of the encoded protein may be a predictive marker of chemoresistant
disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
(provided by RefSeq, Oct 2011)

UniProtKB/Swiss-Prot: ARHGQ_HUMAN, Q96DR7
Function: Activates RhoG GTPase by promoting the exchange of GDP by GTP. Required for the formation of membrane ruffles
during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of
leukocytes. In case of Salmonella enterica infection, activated by SopB, which induces cytoskeleton rearrangements and
promotes bacterial entry




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000003.11  NC_018914.1  NT_005612.16  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for ARHGEF26
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidARHGEF26 promoter sequence
   Search SABiosciences Chromatin IP Primers for ARHGEF26

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ARHGEF26


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3q25.2   Ensembl cytogenetic band:  3q25.2   HGNC cytogenetic band: 3q25.2

ARHGEF26 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ARHGEF26 gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P153839:  view genomic region     (about GC identifiers)

Start:
153,838,792 bp from pter      End:
153,975,616 bp from pter
Size:
136,825 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ARHGQ_HUMAN, Q96DR7 (See protein sequence)
Recommended Name: Rho guanine nucleotide exchange factor 26  
Size: 871 amino acids; 97346 Da
Subunit: Interacts with ICAM1 and RHOG
Subcellular location: Cell projection, ruffle
Sequence caution: Sequence=AAH16628.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
Sequence=AAL27002.1; Type=Miscellaneous discrepancy; Note=Intron retention; Sequence=BAB14159.1; Type=Erroneous
initiation; Note=Translation N-terminally extended; Sequence=BAB14292.1; Type=Erroneous initiation; Note=Translation
N-terminally extended;
Secondary accessions: B3KVP8 E9PBD0 Q68CL1 Q6AZ96 Q6Q8Q8 Q96AW8 Q96DR6 Q9H9D7 Q9H9R2 Q9UFW5
Alternative splicing: 3 isoforms:  Q96DR7-1   Q96DR7-3   Q96DR7-4   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for ARHGEF26: NX_Q96DR7

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q96DR7

  • ARHGEF26 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (3 alternative transcripts): 
    NP_001238891.1  NP_001238892.1  NP_056410.3  

    ENSEMBL proteins: 
     ENSP00000348828   ENSP00000423418   ENSP00000424446   ENSP00000423295  

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    Uscn Proteins for ARHGEF26

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001726ruffle IEA--
    GO:0005622intracellular IEA--


    ARHGEF26 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ARHGEF26 for domains           About GeneDecksing

    4 InterPro domains/families:
     IPR000219 DH-domain
     IPR001849 Pleckstrin_homology
     IPR001452 SH3_domain
     IPR011993 PH_like_dom

    Graphical View of Domain Structure for InterPro Entry Q96DR7

    ProtoNet protein and cluster: Q96DR7

    3 Blocks protein families:
    IPB000219 DH domain
    IPB001452 SH3 domain signature
    IPB001849 Pleckstrin-like


    UniProtKB/Swiss-Prot: ARHGQ_HUMAN, Q96DR7
    Similarity: Contains 1 DH (DBL-homology) domain
    Similarity: Contains 1 PH domain
    Similarity: Contains 1 SH3 domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: ARHGQ_HUMAN, Q96DR7
    Function: Activates RhoG GTPase by promoting the exchange of GDP by GTP. Required for the formation of membrane ruffles
    during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of
    leukocytes. In case of Salmonella enterica infection, activated by SopB, which induces cytoskeleton rearrangements and
    promotes bacterial entry
    Induction: Certain mRNA species appear to be up-regulated by androgens in prostate cancer cells

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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005089Rho guanyl-nucleotide exchange factor activity IEA--
    GO:0005543phospholipid binding IEA--


    ARHGEF26 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for ARHGEF26:
     Increased gamma-H2AX phosphory 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Bacterial invasion of epithelial cells
    Bacterial invasion of epithelial cells1.00

    Pathway sources
    See GeneCards unified pathways
    Show all pathways



    1         Kegg Pathway  (Kegg details for ARHGEF26):
        Bacterial invasion of epithelial cells


    ARHGEF26 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ARHGEF26

    STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

    1 Interacting protein for ARHGEF26 (ENSP000003488284) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RHOGENSP000003394674STRING: ENSP00000339467
    About this table

    Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0035023regulation of Rho protein signal transduction IEA--


    ARHGEF26 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ARHGEF26
    Search CenterWatch for drugs/clinical trials and news about ARHGEF26 / ARHGQ 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ARHGEF26 gene (3 alternative transcripts): 
    NM_001251962.1  NM_001251963.1  NM_015595.3  

    Unigene Cluster for ARHGEF26:

    Rho guanine nucleotide exchange factor (GEF) 26
    Hs.240845  [show with all ESTs]
    Unigene Representative Sequence: NM_001251962
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000356448(uc021xgc.1 uc011bog.1 uc011boh.1) ENST00000465093
    ENST00000496710 ENST00000465817 ENST00000483068(uc011boi.1)

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    hsa-miR-513a-5p hsa-miR-3616-5p hsa-miR-301a hsa-miR-3167 hsa-miR-374a* hsa-miR-374c hsa-miR-3148 hsa-miR-130b
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    Additional cDNA sequence: 

    AB073386.1 AF415175.1 AF415176.1 AK022655.1 AK022884.1 AK123040.1 AK127282.1 AL117429.1 
    AY552599.1 BC016628.1 BC078655.1 

    8 DOTS entries:

    DT.407924  DT.100748311  DT.95322803  DT.101981041  DT.97800976  DT.120912783  DT.97780247  DT.102824403 

    24/113 AceView cDNA sequences (see all 113):

    BX503267 BM987736 BM729068 BM793807 AU125271 CB122093 AA364836 AA425141 
    W96189 BQ446856 AW235054 AW663096 N66657 BX093031 BX502615 BC016628 
    AK127282 BF431051 AF415175 AV725197 AA890400 BI603959 CB047697 BM682002 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ARHGEF26 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CATCTTATGA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See ARHGEF26 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ARHGEF26

    SOURCE GeneReport for Unigene cluster: Hs.240845

    UniProtKB/Swiss-Prot: ARHGQ_HUMAN, Q96DR7
    Tissue specificity: Isoform 1 is broadly expressed, with highest levels in liver (at protein level). Certain mRNA
    species appear to be specifically expressed in prostate and liver

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for ARHGEF26 gene from 5/15 species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Arhgef261 , 5 Rho guanine nucleotide exchange factor (GEF) 261, 5 84.64(n)1
    87.18(a)1
      3 (29.97 cM)5
    6224341  NM_001081295.11  NP_001074764.11 
     623383445 
    chicken
    (Gallus gallus)
    Aves ARHGEF261 Rho guanine nucleotide exchange factor (GEF) 26 76.38(n)
    82.71(a)
      429163  XM_426718.3  XP_426718.3 
    lizard
    (Anolis carolinensis)
    Reptilia ARHGEF266
    --
    73(a)
    1 ↔ 1
    3(16704689-16782830)
    fruit fly
    (Drosophila melanogaster)
    Insecta Exn6
    Ephexin
    10(a)
    1 → many
    3L(16963113-16987488)
    worm
    (Caenorhabditis elegans)
    Secernentea tag-2186
    Temporarily Assigned Gene name family member (tag-...
    19(a)
    1 → many
    II(4004892-4016080)


    ENSEMBL Gene Tree for ARHGEF26 (if available)
    TreeFam Gene Tree for ARHGEF26 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ARHGEF26 gene
    ARHGEF192  ARHGEF152  ARHGEF162  NGEF2  ARHGEF52  
    3 SIMAP similar genes for ARHGEF26 using alignment to 1 protein entry:     ARHGQ_HUMAN:
    ARHGEF16    LOC728377    NGEF

    ARHGEF26 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/2290 NCBI SNPs in ARHGEF26 are shown (see all 2290    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs117206091,2
    H--151228503(+) AGATAA/CCAATA 1 -- us2k1 tfbs30--------
    rs67963921,2
    C,--151230620(+) CTAGCC/TCCAAA 3 -- us2k1 ut51 ese30--------
    rs760170201,2
    F,--151230624(+) CCCCAA/CAACGC 3 -- us2k1 ut511Minor allele frequency- C:0.03WA 118
    rs355256431,2
    C,F,--151230872(+) GGTCCC/TGGACC 3 -- ut51 int12Minor allele frequency- T:0.50NA 4
    rs761300871,2
    C,--151232234(+) GACTCC/TTCAAG 6 L F mis10--------
    rs124948021,2
    H--151232622(+) GATTGA/CTAGTG 3 -- int10--------
    rs730106051,2
    C,--151232781(+) GAGTGA/GGTCTT 3 -- int12Minor allele frequency- G:0.02WA 120
    rs738753221,2
    C,--151232869(+) TTGCTT/CAGAGG 3 -- int12Minor allele frequency- C:0.10WA 120
    rs2012929941,2
    C--151233339(-) AAAGAA/CTCTGC 3 -- int10--------
    rs1147887661,2
    C,F,--151233579(+) TTGAGG/AATTCT 3 -- int11Minor allele frequency- A:0.02WA 118

    HapMap Linkage Disequilibrium report for ARHGEF26 (153838792 - 153975616 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for ARHGEF26: --

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ARHGEF26 for disorders           About GeneDecksing

    6 diseases for ARHGEF26:    About MalaCards
    typhoid fever    hepatoblastoma    ovarian carcinoma    prostate cancer
    prostatitis    carcinoma


    Export disorders for ARHGEF26 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ARHGEF26 gene, integrated from 9 sources (see all 15):
    (articles sorted by number of sources associating them with ARHGEF26)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Isolation of the novel human guanine nucleotide exchange factor Src homology 3 domain-containing guanine nucleotide exchange factor (SGEF) and of C-terminal SGEF, an N-terminally truncated form of SGEF, the expression of which is regulated by androgen in prostate cancer cells. (PubMed id 12697679)1, 2, 3, 9 Qi H.... Labrie C. (2003)
    2. SGEF, a RhoG guanine nucleotide exchange factor that stimulates macropinocytosis. (PubMed id 15133129)1, 2, 3 Ellerbroek S.M.... Burridge K. (2004)
    3. Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas. (PubMed id 15221005)1, 2 Yamada S....Nakagawara A. (2004)
    4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    5. Differential activation and function of Rho GTPases during Salmonella-host cell interactions. (PubMed id 17074883)2, 9 Patel J.C. and Galan J.E. (2006)
    6. The invasive capacity of HPV transformed cells require s the hDlg-dependent enhancement of SGEF/RhoG activity. (PubMed id 22383878)1 Krishna Subbaiah V....Banks L. (2012)
    7. System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. (PubMed id 21406692)2 Rigbolt K.T....Blagoev B. (2011)
    8. Comparative proteomic analysis of advanced serous epi thelial ovarian carcinoma: possible predictors of chemoresistant disease. (PubMed id 21332407)1 Kim S.W....Kim Y.T. (2011)
    9. Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. (PubMed id 19690332)2 Mayya V.... Han D.K. (2009)
    10. RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration. (PubMed id 17875742)2 van Buul J.D....Burridge K. (2007)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 26084 HGNC: 24490 AceView: SGEF Ensembl:ENSG00000114790 euGenes: HUgn26084
    ECgene: ARHGEF26 Kegg: 26084 H-InvDB: ARHGEF26

    (According to HUGE)
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    Patent Information for ARHGEF26 gene:
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