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Aliases for ARHGEF2 Gene

Aliases for ARHGEF2 Gene

  • Rho/Rac Guanine Nucleotide Exchange Factor 2 2 3 5
  • Rho/Rac Guanine Nucleotide Exchange Factor (GEF) 2 2 3
  • Proliferating Cell Nucleolar Antigen P40 3 4
  • Guanine Nucleotide Exchange Factor H1 3 4
  • Microtubule-Regulated Rho-GEF 3 4
  • GEF-H1 3 4
  • LFP40 3 4
  • Rho Guanine Nucleotide Exchange Factor 2 3
  • KIAA0651 4
  • GEFH1 3
  • GEF 3
  • P40 3

External Ids for ARHGEF2 Gene

Previous GeneCards Identifiers for ARHGEF2 Gene

  • GC01M153779
  • GC01M151676
  • GC01M152694
  • GC01M153133
  • GC01M152729
  • GC01M154183
  • GC01M155916
  • GC01M127277

Summaries for ARHGEF2 Gene

Entrez Gene Summary for ARHGEF2 Gene

  • Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]

GeneCards Summary for ARHGEF2 Gene

ARHGEF2 (Rho/Rac Guanine Nucleotide Exchange Factor 2) is a Protein Coding gene. Diseases associated with ARHGEF2 include Unilateral Focal Polymicrogyria and Scott Syndrome. Among its related pathways are Signaling by GPCR and GPCR Pathway. GO annotations related to this gene include transcription factor binding and Rho guanyl-nucleotide exchange factor activity. An important paralog of this gene is AKAP13.

UniProtKB/Swiss-Prot for ARHGEF2 Gene

  • Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2.

Gene Wiki entry for ARHGEF2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ARHGEF2 Gene

Genomics for ARHGEF2 Gene

Regulatory Elements for ARHGEF2 Gene

Enhancers for ARHGEF2 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH01F155965 1.6 FANTOM5 ENCODE 27.6 +31.7 31694 19.0 CREB3L1 MLX ZFP64 FEZF1 DMAP1 SLC30A9 ZNF416 ZNF143 ZNF263 SP3 RIT1 ARHGEF2 PAQR6 KIAA0907 ASH1L RXFP4 SCARNA4 LAMTOR2 UBQLN4 SYT11
GH01F155937 1.8 FANTOM5 Ensembl ENCODE 15.1 +63.9 63851 10.9 HDGF PKNOX1 MLX WRNIP1 ZFP64 ARID4B SIN3A FEZF1 DMAP1 YY1 LOC101928120 RIT1 KIAA0907 ASH1L ARHGEF2 LOC107985210 SNORA80E UBQLN4 LAMTOR2 MSTO2P
GH01F156165 1 FANTOM5 ENCODE 24.5 -158.3 -158308 0.5 BCOR IKZF1 MLLT1 KDM1A SPI1 RELA ARHGEF2 ENSG00000252236 SLC25A44 RIT1 LOC107985210 LMNA MEX3A SEMA4A YY1AP1 MEF2D
GH01F156456 1.6 FANTOM5 ENCODE 11.9 -450.3 -450307 2.7 HDGF PKNOX1 CREB3L1 MLX WRNIP1 ARID4B SIN3A FEZF1 DMAP1 ZNF2 KIAA0907 RRNAD1 ENSG00000271267 LOC246784 SSR2 ENSG00000229961 DAP3P1 TSACC ARHGEF2 C1orf61
GH01F155958 1.1 Ensembl ENCODE 12.7 +45.9 45941 4.4 INSM2 KLF17 SIN3A RAD21 ZNF121 RCOR1 FOS DEK EGR2 SMARCC1 RIT1 LOC107985210 ARHGEF2 KIAA0907 SCARNA4 LAMTOR2 UBQLN4 LOC107985209 MIR6738
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around ARHGEF2 on UCSC Golden Path with GeneCards custom track

Genomic Location for ARHGEF2 Gene

Chromosome:
1
Start:
155,946,640 bp from pter
End:
156,007,070 bp from pter
Size:
60,431 bases
Orientation:
Minus strand

Genomic View for ARHGEF2 Gene

Genes around ARHGEF2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ARHGEF2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ARHGEF2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ARHGEF2 Gene

Proteins for ARHGEF2 Gene

  • Protein details for ARHGEF2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q92974-ARHG2_HUMAN
    Recommended name:
    Rho guanine nucleotide exchange factor 2
    Protein Accession:
    Q92974
    Secondary Accessions:
    • D3DVA6
    • O75142
    • Q15079
    • Q5VY92
    • Q8TDA3
    • Q8WUG4
    • Q9H023

    Protein attributes for ARHGEF2 Gene

    Size:
    986 amino acids
    Molecular mass:
    111543 Da
    Quaternary structure:
    • Found in a complex composed at least of ARHGEF2, NOD2 and RIPK2. Interacts with RIPK2; the interaction mediates tyrosine phosphorylation of RIPK2 by Src kinase CSK. Interacts with RIPK1 and RIPK3. Interacts with YWHAZ/14-3-3 zeta; when phosphorylated at Ser-886. Interacts with the kinases PAK4, AURKA and MAPK1. Interacts with RHOA and RAC1. Interacts with NOD1. Interacts (via the N-terminal zinc finger) with CAPN6 (via domain II) (By similarity).
    SequenceCaution:
    • Sequence=AAC97383.1; Type=Miscellaneous discrepancy; Note=Sequence differs at a large extent from the sequence shown in the paper.; Evidence={ECO:0000305}; Sequence=AAH20567.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAA31626.3; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAA33634.1; Type=Frameshift; Positions=887, 984; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for ARHGEF2 Gene

    Alternative splice isoforms for ARHGEF2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ARHGEF2 Gene

Post-translational modifications for ARHGEF2 Gene

  • Phosphorylation of Ser-886 by PAK1 induces binding to protein YWHAZ, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by AURKA and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules. Phosphorylated on serine, threonine and tyrosine residues in a RIPK2-dependent manner.
  • Ubiquitination at Lys 148 and Lys 438
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for ARHGEF2 Gene

Domains & Families for ARHGEF2 Gene

Protein Domains for ARHGEF2 Gene

Graphical View of Domain Structure for InterPro Entry

Q92974

UniProtKB/Swiss-Prot:

ARHG2_HUMAN :
  • The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA. Promotes tyrosine phosphorylation of RIPK2.
  • Contains 1 phorbol-ester/DAG-type zinc finger.
Domain:
  • The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA. Promotes tyrosine phosphorylation of RIPK2.
  • The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions.
  • Contains 1 DH (DBL-homology) domain.
  • Contains 1 PH domain.
Similarity:
  • Contains 1 phorbol-ester/DAG-type zinc finger.
genes like me logo Genes that share domains with ARHGEF2: view

Function for ARHGEF2 Gene

Molecular function for ARHGEF2 Gene

UniProtKB/Swiss-Prot Function:
Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2.
UniProtKB/Swiss-Prot Induction:
Up-regulated by bacterial peptidoglycans stimulation, such as muramyl dipeptide and in biopsies from inflamed mucosal areas of Crohns disease patients.

Gene Ontology (GO) - Molecular Function for ARHGEF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005085 guanyl-nucleotide exchange factor activity TAS --
GO:0005089 Rho guanyl-nucleotide exchange factor activity IEA,TAS --
GO:0005515 protein binding IPI 14970201
GO:0008017 microtubule binding IDA 9857026
GO:0008134 transcription factor binding ISS --
genes like me logo Genes that share ontologies with ARHGEF2: view
genes like me logo Genes that share phenotypes with ARHGEF2: view

Animal Model Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for ARHGEF2 Gene

Localization for ARHGEF2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ARHGEF2 Gene

Cytoplasm. Cell junction, tight junction. Golgi apparatus. Cytoplasm, cytoskeleton, spindle. Cell projection, ruffle membrane. Cytoplasmic vesicle. Note=Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization. Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells. Colocalized with NOD2 and RIPK2 in vesicles and with the cytoskeleton.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ARHGEF2 gene
Compartment Confidence
plasma membrane 5
cytoskeleton 5
cytosol 5
nucleus 4
golgi apparatus 3

Gene Ontology (GO) - Cellular Components for ARHGEF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002102 podosome IEA --
GO:0005622 intracellular IEA --
GO:0005737 cytoplasm IEA,IDA 21887730
GO:0005794 Golgi apparatus IEA --
GO:0005819 spindle IEA --
genes like me logo Genes that share ontologies with ARHGEF2: view

Pathways & Interactions for ARHGEF2 Gene

genes like me logo Genes that share pathways with ARHGEF2: view

SIGNOR curated interactions for ARHGEF2 Gene

Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for ARHGEF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000132 establishment of mitotic spindle orientation IEA --
GO:0000902 cell morphogenesis IMP 11912491
GO:0002376 immune system process IEA --
GO:0006886 intracellular protein transport NAS 9857026
GO:0007015 actin filament organization IMP 9857026
genes like me logo Genes that share ontologies with ARHGEF2: view

Drugs & Compounds for ARHGEF2 Gene

(15) Drugs for ARHGEF2 Gene - From: ApexBio, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Guanosine triphosphate Experimental Pharma 0
NSC 23766 Pharma Selective inhibitor of Rac1-GEF interaction., Selective inhibitor of Rac1-GEF interaction; antioncogenic 0

(13) Additional Compounds for ARHGEF2 Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Diglycerides Group A
  • 1,2-Dimyristoyl-rac-glycerol
  • DAG(14:0/14:0)
  • DAG(28:0)
  • DG(14:0/14:0)
  • DG(28:0)
Diglycerides Group B
  • 1-Palmitoleoyl-2-arachidonyl-sn-glycerol
  • DAG(16:1/20:0)
  • DAG(16:1n7/20:0)
  • DAG(16:1w7/20:0)
  • DAG(36:1)
Diglycerides Group C
  • 1-Myristoyl-3-stearoyl-sn-glycerol
  • 1-Tetradecanoyl-3-octadecanoyl-sn-glycerol
  • DAG(14:0/0:0/18:0)
  • DAG(32:0)
  • DG(32:0)
Diglycerides Group D
  • 1,2-Didocosahexaenoyl-rac-glycerol
  • DAG(22:6/22:6)
  • DAG(22:6n3/22:6n3)
  • DAG(22:6w3/22:6w3)
  • DAG(44:12)

(1) ApexBio Compounds for ARHGEF2 Gene

Compound Action Cas Number
NSC 23766 Selective inhibitor of Rac1-GEF interaction. 1177865-17-6
genes like me logo Genes that share compounds with ARHGEF2: view

Transcripts for ARHGEF2 Gene

Unigene Clusters for ARHGEF2 Gene

Rho/Rac guanine nucleotide exchange factor (GEF) 2:
Representative Sequences: