ARHGAP8 Gene
protein-coding GIFtS: 47
GCID: GC22P045099
|
|
Rho GTPase activating protein 8
| |
Aliases
for ARHGAP8 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Rho GTPase Activating Protein 81 2 | | PP6102 | | BPGAP11 2 5 | | BCH Domain-Containing Cdc42GAP-Like Protein2 | | Rho-Type GTPase-Activating Protein 82 3 | | BNIP-2 And Cdc42GAP Homology Domain-Containing, Proline-Rich And Cdc42GAP-LikeProtein Subtype-12 | | FLJ201851 | | Rho GTPase-Activating Protein 82 |
Export aliases for ARHGAP8 gene to outside databasesPrevious GC identifers: GC22P041733 GC22P043341 GC22P043349 GC22P043393 GC22P043527 GC22P043451 GC22P045148 GC22P028096 |
Summaries
for ARHGAP8 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for ARHGAP8:
This gene encodes a member of the RHOGAP family. GAP (GTPase-activating) family proteins participate in signalingpathways that regulate cell processes involved in cytoskeletal changes. GAP proteins alternate between an active(GTP-bound) and inactive (GDP-bound) state based on the GTP:GDP ratio in the cell. This family member is a multidomainprotein that functions to promote Erk activation and cell motility. Alternative splicing results in multipletranscript variants. Read-through transcripts from the upstream proline rich 5, renal (PRR5) gene into this gene alsoexist, which led to the original description of PRR5 and ARHGAP8 being a single gene. (provided by RefSeq, Nov 2010)
UniProtKB/Swiss-Prot: RHG08_HUMAN, P85298Function: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state (By similarity)
Gene Wiki entry for ARHGAP8
|
Genomic Views
for ARHGAP8 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the ARHGAP8 gene promoter:
GR RP58 GR-beta HNF-3beta Roaz AREB6 HEN1 ZID GR-alpha Hlf
Other transcription factors
Search SABiosciences Chromatin IP Primers for ARHGAP8
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ARHGAP8 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 22q13.31 Ensembl cytogenetic band: 22q13.31 HGNC cytogenetic band: 22q13.3ARHGAP8 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 22 GeneLoc Exon Structure GeneLoc location for GC22P045099: view genomic region
(about GC identifiers)
Start:
|
45,098,355 bp from pter |
End:
|
45,258,665 bp from pter |
Size:
|
160,311 bases |
Orientation:
|
plus strand |
|
Proteins
for ARHGAP8 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: RHG08_HUMAN, P85298 (See
protein sequence)Recommended Name: Rho GTPase-activating protein 8 Size: 464 amino acids; 53484 Da
Secondary accessions: A6ZJ80 O75983 O95695 Q96RW1 Q96RW2 Q9HA49 Q9HC46 Q9NSG0 Q9NVX8 Q9NXL1 Q9UH20Alternative splicing: 4 isoforms: P85298-1, Q9NSG0-6 P85298-2, Q9NSG0-7 P85298-3 P85298-4 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for ARHGAP8: NX_P85298
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_P85298
ARHGAP8 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (3 alternative transcripts):
NP_001017526.1 NP_001185655.1 NP_851852.2
ENSEMBL proteins: ENSP00000374424 ENSP00000379425 ENSP00000403618 ENSP00000337287 ENSP00000348407 ENSP00000402775 ENSP00000374422 ENSP00000429240 ENSP00000374423 Reactome Protein details: P85298
Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table
ARHGAP8 for ontologies About GeneDecksing
ARHGAP8 Antibody Products:
Assay Products for ARHGAP8: |
Protein
Domains /
Families
for ARHGAP8 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
ARHGAP8 for domains About GeneDecksing
3 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry P85298ProtoNet protein and cluster: P85298 UniProtKB/Swiss-Prot: RHG08_HUMAN, P85298Similarity: Contains 1 CRAL-TRIO domainSimilarity: Contains 1 Rho-GAP domain |
Function
for ARHGAP8 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: RHG08_HUMAN, P85298Function: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state (By similarity) Genatlas biochemistry entry for ARHGAP8:Rho GTPase activating protein 8
Clone
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ARHGAP8 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005100 | Rho GTPase activator activity |
IDA | -- | | GO:0005515 | protein binding |
IPI | -- |
ARHGAP8 for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for ARHGAP8:
|
Pathways & Interactions
for ARHGAP8 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Cell death signalling via NRAGE, NRIF and NADE | | | 2 | Regulation of RhoA activity | | | 3 | Signaling by GPCR | |
Pathway sources
See GeneCards unified pathways
Show all pathways
1  BioSystems Pathway for ARHGAP8
3 
Reactome Pathways for ARHGAP8
ARHGAP8 for pathways About GeneDecksing
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for ARHGAP8
STRING Interaction
Network Preview (showing 5 interactants - click image to see 6)
5/8 Interacting proteins for ARHGAP8 (P852983 ENSP000003744244) via UniProtKB, MINT, STRING, and/or I2D (see all 8)About this table
Gene Ontology (GO): 5 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0007165 | signal transduction |
-- | -- | | GO:0007264 | small GTPase mediated signal transduction |
TAS | -- | | GO:0032321 | positive regulation of Rho GTPase activity |
IDA | -- | | GO:0051056 | regulation of small GTPase mediated signal transduction |
TAS | -- | | GO:0070374 | positive regulation of ERK1 and ERK2 cascade |
IDA | -- |
ARHGAP8 for ontologies About GeneDecksing
|
Drugs & Compounds
for ARHGAP8 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for ARHGAP8
Search CenterWatch for drugs/clinical trials and news about ARHGAP8 / RHG08
|
Transcripts
for ARHGAP8 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for ARHGAP8 gene (3 alternative transcripts): NM_001017526.1 NM_001198726.1 NM_181335.2 14 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000389774(uc010gzv.3 uc003bfj.3 uc003bfk.3) ENST00000460809(uc003bfi.3)
ENST00000396119 ENST00000447333 ENST00000336963 ENST00000356099 ENST00000412433
ENST00000469872 ENST00000389772(uc003bfl.3) ENST00000495219 ENST00000498310
ENST00000469342 ENST00000517296(uc003bfd.3) ENST00000389773(uc011aqi.2 uc011aqj.2)
Clone
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OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ARHGAP8 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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Synthesis Service for ARHGAP8 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ARHGAP8 |
24/276 AceView cDNA sequences (see all 276): CR622409 BX336422 NM_015366 R69153 BE887282 H55372 CR613509 BX096591 BC067824 AI589661 CA313656 CD559077 NM_181334 AI143284 CR594267 CR598211 BF724807 AU121932 CR618913 CR611486 AW673087 BX345142 BG170601 AF195969
GeneLoc Exon Structure
|
Expression
for ARHGAP8 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| ARHGAP8 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See ARHGAP8 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for ARHGAP8
UniProtKB/Swiss-Prot: RHG08_HUMAN, P85298Tissue specificity: Highly expressed in kidney and placenta. Also expressed in colon, skeletal muscle, small intestine,stomach, and testis. Not detected in brain, liver or spleen. Overexpressed in the majority of colorectal tumorsexamined
SABiosciences Custom PCR Arrays for ARHGAP8
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for ARHGAP8
Browse OriGene validated miRNA SYBR primer pairs
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Primer Assays in human, mouse, rat ARHGAP8 | | | Search QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat ARHGAP8 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ARHGAP8 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ARHGAP8 |
Orthologs
for ARHGAP8 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for ARHGAP8 gene from 5/18 species (see all 18) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
ARHGAP81 |
Rho GTPase activating protein 8 |
69.17(n)
75.65(a) |
|
418239 NM_001204389.1 NP_001191318.1 |
lizard
(Anolis carolinensis) |
Reptilia |
-- |
-- |
66(a) |
1 → many |
5(84941827-84966554) |
African clawed frog
(Xenopus laevis) |
Amphibia |
arhgap8-prov2 |
Rho GTPase activating protein 8 |
76.55(n) |
|
BC042252.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
RhoGAP68F3 |
Rho GTPase activator |
34(a) |
|
-- |
worm
(Caenorhabditis elegans) |
Secernentea |
W02B12.83 |
GTPase-activating (rhoGAP) like protein |
36(a) |
|
II(11464575-11466218) -- |
ENSEMBL Gene Tree for ARHGAP8 (if available)
TreeFam Gene Tree for ARHGAP8 (if available) |
Paralogs
for ARHGAP8 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for ARHGAP8 gene
- PRR5-ARHGAP81 2 PRUNE22 BNIP22 ATCAY2 ARHGAP12 BNIPL2
13 SIMAP similar genes for ARHGAP8 using alignment to 12 protein entries: RHG08_HUMAN (see all proteins):BPGAP1 LOC553158 PRR5-ARHGAP8 PRR5 ARHGAP1 ARHGAP12
FAM13A BNIP2 ARHGAP9 PRUNE2 ATCAY BNIPL
GMIP
ARHGAP8 for paralogs About GeneDecksing
|
Genomic Variants
for ARHGAP8 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 22 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for ARHGAP8 (45098355 - 45258665 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 2 variations for ARHGAP8
2 CNVs: 103191 4131 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ARHGAP8 |
|
Disorders
/ Diseases
for ARHGAP8 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
ARHGAP8 for disorders About GeneDecksing
OMIM gene information: 609405
OMIM disorders: --
6  diseases for ARHGAP8: About MalaCardscervix carcinoma hematopoiesis cervical cancer cervicitis
breast cancer carcinoma
Human Genome Epidemiology (HuGE) Navigator: ARHGAP8 (4 documents)
Export disorders for ARHGAP8 gene to outside databases
|
Publications
for ARHGAP8 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for ARHGAP8 gene, integrated from 9 sources (see all 19):
(articles sorted by number of sources associating them with ARHGAP8) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The DNA sequence of human chromosome 22. (PubMed id 10591208)1, 2, 3 Dunham I....O'Brien K.P. (1999)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- ARHGAP8 is a novel member of the RHOGAP family related to ARHGAP1/CDC42GAP/p50RHOGAP: mutation and expression analyses in colorectal and breast cancers. (PubMed id 15225876)1, 2 Johnstone C.N.... Rustgi A.K. (2004)
- Activation of EGF receptor endocytosis and ERK1/2 signaling by BPGAP1 requires direct interaction with EEN/endophilin II and a functional RhoGAP domain. (PubMed id 15944398)1, 9 Lua B.L. and Low B.C. (2005)
- Active Mek2 as a regulatory scaffold that promotes Pi n1 binding to BPGAP1 to suppress BPGAP1-induced acute Erk activation and cell m igration. (PubMed id 20179103)1, 9 Pan C.Q....Low B.C. (2010)
- Concerted regulation of cell dynamics by BNIP-2 and Cdc42GAP homology/Sec14p-like, proline-rich, and GTPase-activating protein domains of a novel Rho GTPase-activating protein, BPGAP1. (PubMed id 12944407)1, 9 Shang X.... Low B.C. (2003)
- Identification and characterization of a gene encoding a putative mouse Rho GTPase activating protein gene 8, Arhgap8. (PubMed id 12559566)1, 9 Shan Z....Popescu N.C. (2003)
- Family-based genome-wide association study of frontal theta oscillations identifies potassium channel gene KCNJ6. (PubMed id 22554406)1 Kang S.J....Porjesz B. (2012)
- Genome-wide meta-analyses of nonsyndromic cleft lip wi th or without cleft palate identify six new risk loci. (PubMed id 22863734)1 Ludwig K.U....Nothen M.M. (2012)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
|
External Searches for ARHGAP8 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing ARHGAP8 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing ARHGAP8 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing ARHGAP8 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for ARHGAP8 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for ARHGAP8 gene:
Search GeneIP for patents involving ARHGAP8
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for ARHGAP8 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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