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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ARHGAP25 Gene

protein-coding   GIFtS: 50
GCID: GC02P068906

Rho GTPase activating protein 25

 Explore 1 disease affiliated with
ARHGAP25 via our new
 Human Malady Compendium 
Biological research products
for ARHGAP25
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Rho GTPase Activating Protein 251 2
KIAA00531 3
Rho-Type GTPase-Activating Protein 252 3
KAIA00532 5
Rho GTPase-Activating Protein 252

External Ids:    HGNC: 289511   Entrez Gene: 99382   Ensembl: ENSG000001632197   OMIM: 6105875   UniProtKB: P423313   

Export aliases for ARHGAP25 gene to outside databases

Previous GC identifers: GC02P068936 GC02P068913 GC02P068698


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ARHGAP25:
ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in
actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 (PubMed 15254788)).(supplied by OMIM, Mar
2008)

UniProtKB/Swiss-Prot: RHG25_HUMAN, P42331
Function: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state (By similarity)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000002.11  NC_018913.1  NT_022184.15  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ARHGAP25 gene promoter:
         HOXA9B   HOXA9   CUTL1   Evi-1   PPAR-gamma1   PPAR-gamma2   Meis-1a   Pax-4a   Meis-1   ATF6   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): ARHGAP25 promoter sequence
   Search SABiosciences Chromatin IP Primers for ARHGAP25

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ARHGAP25


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2p13.3   Ensembl cytogenetic band:  2p13.3   HGNC cytogenetic band: 2p13.3

ARHGAP25 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ARHGAP25 gene location

GeneLoc information about chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P068906:  view genomic region     (about GC identifiers)

Start:
68,906,733 bp from pter      End:
69,053,965 bp from pter
Size:
147,233 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: RHG25_HUMAN, P42331 (See protein sequence)
Recommended Name: Rho GTPase-activating protein 25  
Size: 645 amino acids; 73435 Da
Sequence caution: Sequence=BAA06125.2; Type=Erroneous initiation;
1 PDB 3D structure from and Proteopedia for ARHGAP25:
1V89 (3D)    
Secondary accessions: A8K2Y1 B7Z498 E9PFQ7 Q8IXQ2
Alternative splicing: 5 isoforms:  P42331-1   P42331-2   P42331-3   P42331-4   P42331-5   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for ARHGAP25: NX_P42331

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_P42331

  • ARHGAP25 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (4 alternative transcripts): 
    NP_001007232.2  NP_001159748.1  NP_001159749.1  NP_055697.1  

    ENSEMBL proteins: 
     ENSP00000417283   ENSP00000386911   ENSP00000420583   ENSP00000420427   ENSP00000386863  
     ENSP00000386241   ENSP00000417425   ENSP00000417139   ENSP00000417818   ENSP00000417467  
     ENSP00000295381   ENSP00000439917  
    Reactome Protein details: P42331
    Human Recombinant Protein Products: 
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    Novus Biologicals ARHGAP25 Protein
    Novus Biologicals ARHGAP25 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for ARHGAP25

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005829cytosol TAS--


    ARHGAP25 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ARHGAP25 for domains           About GeneDecksing

    4 InterPro domains/families:
     IPR008936 Rho_GTPase_activation_prot
     IPR001849 Pleckstrin_homology
     IPR011993 PH_like_dom
     IPR000198 RhoGAP_dom

    Graphical View of Domain Structure for InterPro Entry P42331

    ProtoNet protein and cluster: P42331

    2 Blocks protein families:
    IPB000198 RhoGAP domain
    IPB001849 Pleckstrin-like


    UniProtKB/Swiss-Prot: RHG25_HUMAN, P42331
    Similarity: Contains 1 PH domain
    Similarity: Contains 1 Rho-GAP domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: RHG25_HUMAN, P42331
    Function: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state (By similarity)

    miRNA
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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005096GTPase activator activity IEA--
    GO:0005543phospholipid binding IEA--


    ARHGAP25 for ontologies           About GeneDecksing


    Animal Models:
         2 MGI mutant phenotypes (inferred from 1 allele(MGI details for Arhgap25):
     pigmentation  vision/eye 

    ARHGAP25 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Cell death signalling via NRAGE, NRIF and NADE
    Signaling by Rho GTPases0.25
    Rho GTPase cycle0.25
    2Signaling by GPCR
    Signal Transduction0.56

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    3        Reactome Pathways for ARHGAP25
        Signaling by Rho GTPases
    Signal Transduction
    Rho GTPase cycle



    ARHGAP25 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ARHGAP25

    Gene Ontology (GO): 3 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007165signal transduction ----
    GO:0007264small GTPase mediated signal transduction TAS--
    GO:0051056regulation of small GTPase mediated signal transduction TAS--


    ARHGAP25 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ARHGAP25
    Search CenterWatch for drugs/clinical trials and news about ARHGAP25 / RHG25 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ARHGAP25 gene (4 alternative transcripts): 
    NM_001007231.2  NM_001166276.1  NM_001166277.1  NM_014882.2  

    Unigene Cluster for ARHGAP25:

    Rho GTPase activating protein 25
    Hs.531807  [show with all ESTs]
    Unigene Representative Sequence: NM_001007231
    18/19 Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 19):
    ENST00000463483(uc010yqk.2) ENST00000481684 ENST00000491237 ENST00000409202
    ENST00000456116 ENST00000467265 ENST00000485573 ENST00000488795 ENST00000463061
    ENST00000485700 ENST00000496266 ENST00000409030(uc002sew.3) ENST00000409220(uc002sex.3)
    ENST00000473986(uc010fdh.1) ENST00000497079(uc002sev.3) ENST00000497259
    ENST00000479844 ENST00000295381(uc010fdg.3 uc010yql.2)

    miRNA
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    2 QIAGEN miScript miRNA Assays for microRNAs that regulate ARHGAP25:
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    Inhib. RNA
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    Additional cDNA sequence: 

    AK290396.1 AK297056.1 AK297157.1 AK298669.1 AK303591.1 AK310742.1 AK311669.1 BC032222.1 
    BC039591.1 D29642.1 

    11 DOTS entries:

    DT.313185  DT.97822313  DT.97789708  DT.100776740  DT.102828731  DT.91855476  DT.100697288  DT.120988870 
    DT.120988886  DT.120988896  DT.120988909 

    24/80 AceView cDNA sequences (see all 80):

    AI612855 BQ067474 BQ073007 BI518793 BC039591 BU176833 AA811916 BM988983 
    CR607155 AI675462 AA280922 D29642 AA554388 AI567925 CR603706 BC032222 
    BI030380 AA280844 CR617270 BX101295 AI434357 T56421 BF914207 AW235472 

    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for ARHGAP25 (see all 6)    About this scheme

    ExUns: 1a · 1b · 1c · 1d · 1e · 1f ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8 ^ 9a · 9b ^ 10 ^ 11
    SP1:                                      -                                                           -               
    SP2:                                      -                                                                           
    SP3:                                                  -                                                               
    SP4:                                      -           -                                                               
    SP5:                                -                                                                                 


    ECgene alternative splicing isoforms for ARHGAP25

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ARHGAP25 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: --

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See ARHGAP25 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ARHGAP25

    SOURCE GeneReport for Unigene cluster: Hs.531807
        SABiosciences Custom PCR Arrays for ARHGAP25
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ARHGAP25

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for ARHGAP25 gene from 3/15 species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves ARHGAP251 Rho GTPase activating protein 25 68.77(n)
    65.7(a)
      419516  NM_001030883.1  NP_001026054.1 
    lizard
    (Anolis carolinensis)
    Reptilia ARHGAP256
    --
    45(a)
    1 ↔ 1
    AAWZ02040531(1406-5564)
    zebrafish
    (Danio rerio)
    Actinopterygii LOC5624681 novel protein similar to vertebrate Rho GTPase activating more 53.94(n)
    47.64(a)
      562468  NM_001126404.1  NP_001119876.1 


    ENSEMBL Gene Tree for ARHGAP25 (if available)
    TreeFam Gene Tree for ARHGAP25 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ARHGAP25 gene
    SRGAP12  ARHGAP52  ARHGAP172  ARHGAP242  ARHGAP222  ARHGAP352  SRGAP32  ARHGAP442  
    SH3BP12  ARHGAP42  SRGAP22  
    8 SIMAP similar genes for ARHGAP25 using alignment to 7 protein entries:     RHG25_HUMAN (see all proteins):
    KIAA0053    ARHGAP22    ARHGAP24    FAM13A    CHN2    ARHGAP21
    ARHGAP31    FAM109A

    ARHGAP25 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/1819 NCBI SNPs in ARHGAP25 are shown (see all 1819    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 2 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1389969321,2
    --68959928(+) AATGCG/TCTTGC 2 -- us2k10--------
    rs1835635481,2
    --68959943(+) TTCTTC/TTCTCT 2 -- us2k10--------
    rs779404901,2
    --68959995(+) CACCAC/GTGTTC 2 -- us2k12Minor allele frequency- G:0.04CSA WA 120
    rs1869669481,2
    --68960010(+) AGCCTC/TGTTCT 2 -- us2k10--------
    rs746141201,2
    F,--68960037(+) GATACA/GTTTAG 2 -- us2k11Minor allele frequency- G:0.05WA 118
    rs1910549541,2
    --68960087(+) GATTTG/TGAGTT 2 -- us2k10--------
    rs351051751,2
    C,F,--68960335(+) CATGGA/GCAGAG 2 -- us2k16Minor allele frequency- G:0.10NA EA 248
    rs1430245461,2
    --68960364(+) GCTATC/GAAATG 2 -- us2k10--------
    rs1474714091,2
    --68960374(+) GTGTCC/TGACAC 2 -- us2k10--------
    rs758414011,2
    C,F,--68960485(+) AGGGGC/AAAAAA 2 -- us2k15Minor allele frequency- A:0.18CSA WA NA EA 362

    HapMap Linkage Disequilibrium report for ARHGAP25 (68906733 - 69053965 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for ARHGAP25: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ARHGAP25
    DNA2.0 Custom Variant and Variant Library Synthesis for ARHGAP25

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ARHGAP25 for disorders           About GeneDecksing

    OMIM gene information: 610587    OMIM disorders: --

    1 disease for ARHGAP25:    About MalaCards
    spasticity

    Human Genome Epidemiology (HuGE) Navigator: ARHGAP25 (1 document)

    Export disorders for ARHGAP25 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ARHGAP25 gene integrated from 9 sources:
    (articles sorted by number of sources associating them with ARHGAP25)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1. (PubMed id 7584044)1, 2, 3 Nomura N....Tabata S. (1994)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Identification and characterization of ARHGAP24 and ARHGAP25 genes in silico. (PubMed id 15254788)1, 9 Katoh M. and Katoh M. (2004)
    4. Mutation of ARHGAP9 in patients with coronary spastic angina. (PubMed id 19911011)1 Takefuji M....Kaibuchi K. (2010)
    5. Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. (PubMed id 19690332)2 Mayya V.... Han D.K. (2009)
    6. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)2 Hillier L.W....Wilson R.K. (2005)
    7. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (2004)
    8. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 9938 HGNC: 28951 AceView: ARHGAP25 Ensembl:ENSG00000163219 euGenes: HUgn9938
    ECgene: ARHGAP25 H-InvDB: ARHGAP25

    (According to HUGE)
    About This Section
    HUGE: KIAA0053

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for ARHGAP25 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for ARHGAP25 gene:
    Search GeneIP for patents involving ARHGAP25

    GeneCards and IP:
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