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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ARFIP1 Gene

protein-coding   GIFtS: 53
GCID: GC04P153701

ADP-ribosylation factor interacting protein 1

 Explore 4 diseases affiliated with
ARFIP1 via our new
 Human Malady Compendium 
Biological research products
for ARFIP1
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
ADP-Ribosylation Factor Interacting Protein 11 2
HSU525211 2
ADP-Ribosylation Factor-Interacting Protein 12 3
Arfaptin 12
Arfaptin-11

External Ids:    HGNC: 214961   Entrez Gene: 272362   Ensembl: ENSG000001641447   OMIM: 6059285   UniProtKB: P533673   

Export aliases for ARFIP1 gene to outside databases

Previous GC identifers: GC04P154167 GC04P154278 GC04P154058 GC04P153920 GC04P149427


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: ARFP1_HUMAN, P53367
Function: Putative target protein of ADP-ribosylation factor

Gene Wiki entry for ARFIP1


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000004.11  NC_018915.1  NT_016354.19  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ARFIP1 gene promoter:
         STAT1   STAT1beta   FOXD3   XBP-1   STAT1alpha   MEF-2A   HFH-1   HSF2   Chx10   aMEF-2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidARFIP1 promoter sequence
   Search SABiosciences Chromatin IP Primers for ARFIP1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ARFIP1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q31.3   Ensembl cytogenetic band:  4q31.3   HGNC cytogenetic band: 4q31.3

ARFIP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ARFIP1 gene location

GeneLoc information about chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P153701:  view genomic region     (about GC identifiers)

Start:
153,701,089 bp from pter      End:
153,839,615 bp from pter
Size:
138,527 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ARFP1_HUMAN, P53367 (See protein sequence)
Recommended Name: Arfaptin-1  
Size: 373 amino acids; 41738 Da
Subunit: Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower
affinity to ARF5 and with very little affinity to ARF6
Secondary accessions: Q2M2X4 Q3SYL4 Q9Y2X6
Alternative splicing: 2 isoforms:  P53367-1   P53367-2   

Explore the universe of human proteins at neXtProt for ARFIP1: NX_P53367

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_P53367

  • ARFIP1 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (3 alternative transcripts): 
    NP_001020764.1  NP_001020766.1  NP_055262.1  

    ENSEMBL proteins: 
     ENSP00000395083   ENSP00000396653   ENSP00000384189   ENSP00000348360   ENSP00000296557  

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    Novus Biologicals ARFIP1 Proteins
    Novus Biologicals ARFIP1 Lysates
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    Uscn Proteins for ARFIP1

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000139Golgi membrane IDA9038142
    GO:0005829cytosol IDA9038142


    ARFIP1 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ARFIP1 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR010504 AH_dom

    Graphical View of Domain Structure for InterPro Entry P53367

    ProtoNet protein and cluster: P53367

    1 Blocks protein family: IPB010504 Arfaptin-like

    UniProtKB/Swiss-Prot: ARFP1_HUMAN, P53367
    Similarity: Contains 1 AH domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: ARFP1_HUMAN, P53367
    Function: Putative target protein of ADP-ribosylation factor

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ARFIP1

    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003674molecular_function ND--
    GO:0019904protein domain specific binding IEA--
    GO:0042802identical protein binding IEA--


    ARFIP1 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for ARFIP1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 9)

    5/12 Interacting proteins for ARFIP1 (P533672, 3 ENSP000002965574) via UniProtKB, MINT, STRING, and/or I2D (see all 12)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ARF1P840773, ENSP000002721024I2D: score=2 STRING: ENSP00000272102
    ARF3P612043, ENSP000002566824I2D: score=2 STRING: ENSP00000256682
    ARF5P840853, ENSP000000002334I2D: score=1 STRING: ENSP00000000233
    EYA2O001673I2D: score=3 
    SLC2A4P146723I2D: score=3 
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006886intracellular protein transport IDA12606037
    GO:0050708regulation of protein secretion IDA12606037


    ARFIP1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
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    Browse Tocris compounds for ARFIP1

    1 HMDB Compound for ARFIP1    About this table
    CompoundSynonyms CAS #PubMed Ids
    Guanosine triphosphate5'-GTP (see all 10)86-01-1--
    Search CenterWatch for drugs/clinical trials and news about ARFIP1 / ARFP1 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ARFIP1 gene (3 alternative transcripts): 
    NM_001025593.1  NM_001025595.1  NM_014447.2  

    Unigene Cluster for ARFIP1:

    ADP-ribosylation factor interacting protein 1
    Hs.416089  [show with all ESTs]
    Unigene Representative Sequence: NM_001025595
    11 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000451320(uc003inc.3) ENST00000429148 ENST00000405727 ENST00000356064
    ENST00000510831 ENST00000509413 ENST00000511289 ENST00000513361 ENST00000514499
    ENST00000510497 ENST00000353617(uc003imz.3 uc003inb.3 uc003ina.3 uc011cij.2)


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    8/28 QIAGEN miScript miRNA Assays for microRNAs that regulate ARFIP1 (see all 28):
    hsa-miR-320a hsa-miR-148b hsa-miR-2110 hsa-miR-301a hsa-miR-373* hsa-miR-548l hsa-miR-223 hsa-miR-124
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    Inhib. RNA
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    Additional cDNA sequence: 

    AF124489.1 AK096509.1 AK290472.1 AK299600.1 AK304141.1 AK316125.1 BC103759.1 BC105114.1 
    BC105116.1 BC143675.1 BC143676.1 U52521.1 

    11 DOTS entries:

    DT.95164065  DT.86853897  DT.315763  DT.91764443  DT.121269321  DT.121269305  DT.121269311  DT.121269329 
    DT.40131068  DT.99976018  DT.99988510 

    24/155 AceView cDNA sequences (see all 155):

    AI753308 AI015024 AI498016 AA838781 AA345528 NM_014447 AI653822 BF221701 
    CB216446 CD643329 N22390 BE350335 BM769127 AA971915 AI360802 CR602975 
    AF124489 AA886668 U52521 AI097610 R27400 AA678866 BU166318 CB269796 

    GeneLoc Exon Structure

    5/14 Alternative Splicing Database (ASD) splice patterns (SP) for ARFIP1 (see all 14)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b · 8c ^ 9 ^ 10 ^ 11 ^ 12a · 12b · 12c · 12d ^ 13a · 13b · 13c · 13d · 13e ^ 14 ^ 15 ^
    SP1:                    -     -     -     -     -     -                 -     -                                                                             -   
    SP2:                    -     -     -     -     -     -                 -     -           -                                                                 -   
    SP3:                          -     -     -     -     -                 -     -           -                 -     -     -                                   -   
    SP4:                          -     -     -     -     -                 -     -                                         -     -                                 
    SP5:                          -     -     -     -     -                 -     -                                                                                 

    ExUns: 16 ^ 17 ^ 18a · 18b · 18c · 18d
    SP1:  -                                 
    SP2:  -                                 
    SP3:  -                                 
    SP4:                                    
    SP5:                                    


    ECgene alternative splicing isoforms for ARFIP1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ARFIP1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: AACTTTGTGG

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See ARFIP1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ARFIP1

    SOURCE GeneReport for Unigene cluster: Hs.416089

    UniProtKB/Swiss-Prot: ARFP1_HUMAN, P53367
    Tissue specificity: Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart

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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for ARFIP1 gene from 7/23 species (see all 23)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Arfip11 , 5 ADP-ribosylation factor interacting protein 11, 5 89.81(n)1
    92.49(a)1
      3 (37.70 cM)5
    998891  NM_001081093.11  NP_001074562.11 
     844960935 
    chicken
    (Gallus gallus)
    Aves ARFIP11 ADP-ribosylation factor interacting protein 1 84.19(n)
    90.03(a)
      770884  NM_001252398.1  NP_001239327.1 
    lizard
    (Anolis carolinensis)
    Reptilia ARFIP16
    --
    90(a)
    1 ↔ 1
    5(131439301-131471642)
    African clawed frog
    (Xenopus laevis)
    Amphibia loc60382-prov2 arfaptin 1 78.07(n)    BC045010.1 
    zebrafish
    (Danio rerio)
    Actinopterygii idibd20772 Danio rerio cDNA clone MGC66341 IMAGE5603861, complete more 77.43(n)    BC054687.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG171846
    --
    43(a)
    1 → many
    3R(7058151-7060907)
    worm
    (Caenorhabditis elegans)
    Secernentea F54C8.76
    Uncharacterized protein F54C8.7
    29(a)
    1 → many
    III(9451507-9453980)


    ENSEMBL Gene Tree for ARFIP1 (if available)
    TreeFam Gene Tree for ARFIP1 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ARFIP1 gene
    ARFIP22  
    1 SIMAP similar gene for ARFIP1 using alignment to 3 protein entries:     ARFP1_HUMAN (see all proteins):
    ARFIP2

    ARFIP1 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 2 variations for ARFIP1
         2 CNVs: 51275 51276
    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ARFIP1
    DNA2.0 Custom Variant and Variant Library Synthesis for ARFIP1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ARFIP1 for disorders           About GeneDecksing

    OMIM gene information: 605928    OMIM disorders: --

    4 diseases for ARFIP1:    About MalaCards
    intrahepatic cholangiocarcinoma    cholangiocarcinoma    fibrosarcoma    cholera

    1 disease from the University of Copenhagen DISEASES database for ARFIP1:
    Cholera

    Export disorders for ARFIP1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ARFIP1 gene, integrated from 9 sources (see all 23):
    (articles sorted by number of sources associating them with ARFIP1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Arfaptin 1 forms a complex with ADP-ribosylation factor and inhibits phospholipase D. (PubMed id 10413101)1, 2, 3, 9 Williger B.-T.... Exton J.H. (1999)
    2. Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes. (PubMed id 9038142)1, 2, 3 Kanoh H.... Exton J.H. (1997)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    5. Arfaptin 1 inhibits ADP-ribosylation factor-dependent matrix metalloproteinase-9 secretion induced by phorbol ester in HT 1080 fibrosarcoma cells. (PubMed id 12606037)1, 9 Ho W.T....Williger B.T. (2003)
    6. A high-throughput approach for measuring temporal chan ges in the interactome. (PubMed id 22863883)1 Kristensen A.R....Foster L.J. (2012)
    7. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    8. Activity-based chemical proteomics accelerates inhibi tor development for deubiquitylating enzymes. (PubMed id 22118674)1 Altun M....Kessler B.M. (2011)
    9. A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
    10. Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 27236 HGNC: 21496 AceView: ARFIP1 Ensembl:ENSG00000164144 euGenes: HUgn27236
    ECgene: ARFIP1 H-InvDB: ARFIP1

    (According to HUGE)
    About This Section
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
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    About This Section
    NameDescription
    PharmGKB entry for ARFIP1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
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    Patent Information for ARFIP1 gene:
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