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ARF6 Gene

protein-coding   GIFtS: 67
GCID: GC14P050359

ADP-Ribosylation Factor 6

  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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This gene clusters with an RNA gene
Subcategory (RNA class): lncRNA

Quality score for the ORGUL clustered with this gene is 3.2

Aliases
ADP-Ribosylation Factor 61 2

External Ids:    HGNC: 6591   Entrez Gene: 3822   Ensembl: ENSG000001655277   OMIM: 6004645   UniProtKB: P623303   
ORGUL members:    fRNAdb10:FR208801 FR383455      
RNAdb15:RNZ11201 RNZ11202    

Export aliases for ARF6 gene to outside databases

Previous GC identifers: GC14P047681 GC14P044155 GC14P048349 GC14P049429 GC14P030484


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for ARF6 Gene:
This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes
encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera
toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is
localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and
signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. (provided
by RefSeq, Jul 2008)

GeneCards Summary for ARF6 Gene:
ARF6 (ADP-ribosylation factor 6) is a protein-coding gene. Diseases associated with ARF6 include familial infantile myoclonic epilepsy, and cholera. GO annotations related to this gene include GTP binding and thioesterase binding. An important paralog of this gene is ARL2.

UniProtKB/Swiss-Prot: ARF6_HUMAN, P62330
Function: GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton
remodeling. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the
actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the
Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of
dendritic branching and filopodia extension. Functions as an allosteric activator of the cholera toxin catalytic
subunit, an ADP-ribosyltransferase

Gene Wiki entry for ARF6 Gene

fRNAdb sequence ontology for ARF6:
nc_conserved_region - Non-coding region of sequence similarity by descent from a common ancestor.

View fRNAdb secondary structures for ARF6

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000014.9  NC_018925.2  NT_026437.13  
Regulatory elements:
   Regulatory transcription factor binding sites in the ARF6 gene promoter:
         HOXA9   AP-1   ATF-2   USF-1:USF-2   Nkx2-5   AREB6   USF-1   c-Jun   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidARF6 promoter sequence
   Search Chromatin IP Primers for ARF6

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ARF6


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 14q21.3   Ensembl cytogenetic band:  14q21.3   HGNC cytogenetic band: 14q21.3

ARF6 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ARF6 gene location

GeneLoc information about chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14P050359:  view genomic region     (about GC identifiers)

Start:
50,359,736 bp from pter      End:
50,363,772 bp from pter
Size:
4,037 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: ARF6_HUMAN, P62330 (See protein sequence)
Recommended Name: ADP-ribosylation factor 6  
Size: 175 amino acids; 20082 Da
Subunit: Interacts with ARHGAP21, ASAP2, ASAP3, HERC1, PIP5K1C and UACA. The interaction with ASAP3 is stabilized
by calcium ions. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3 and RAB11FIP4.
Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24. Interacts with
MICALL1. Interacts with KIF23, forming heterodimers and heterotetramers. Interacts with GGA1 and RAB11FIP4.
Interacts with SPAG9 homodimers, forming heterotetramers. Interacts with CYTH3. Interacts with EspG from
enteropathogenic E.coli. Identified in a complex with RAB1A and EspG from enteropathogenic E.coli. Interacts with
the V.cholerae enterotoxin subunit A1; this causes a conformation change so that the toxin can bind NAD and
catalyze the ADP-ribosylation of Gs alpha
Selected PDB 3D structures from and Proteopedia for ARF6 (see all 14):
1E0S (3D)        2A5D (3D)        2A5F (3D)        2A5G (3D)        2BAO (3D)        2BAU (3D)    
Secondary accessions: P26438 Q6FGZ2

Explore the universe of human proteins at neXtProt for ARF6: NX_P62330

Explore proteomics data for ARF6 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys58, Lys69
  • Modification sites at PhosphoSitePlus

  • See ARF6 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_001654.1  
    ENSEMBL proteins: 
     ENSP00000298316  

    ARF6 Human Recombinant Protein Products:

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    LSBio Antibodies in human, mouse, rat for ARF6

    ARF6 Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    GenScript Custom Assay Services for ARF6
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for ARF6
    Cloud-Clone Corp. CLIAs for ARF6


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    ARF: ADP-ribosylation factors

    4 InterPro protein domains:
     IPR005225 Small_GTP-bd_dom
     IPR027417 P-loop_NTPase
     IPR006689 Small_GTPase_ARF/SAR
     IPR024156 Small_GTPase_ARF

    Graphical View of Domain Structure for InterPro Entry P62330

    ProtoNet protein and cluster: P62330

    UniProtKB/Swiss-Prot: ARF6_HUMAN, P62330
    Similarity: Belongs to the small GTPase superfamily. Arf family


    ARF6 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ARF6_HUMAN, P62330
    Function: GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton
    remodeling. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the
    actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the
    Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of
    dendritic branching and filopodia extension. Functions as an allosteric activator of the cholera toxin catalytic
    subunit, an ADP-ribosyltransferase
    Enzyme regulation: Activation is generally mediated by a guanine exchange factor (GEF), while inactivation through
    hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). Activated by ASAP3. Inactivated by
    ACAP1 and ACAP2

         Genatlas biochemistry entry for ARF6:
    ADP ribosylation factor 6,class III,low molecular weight,widely expressed,involved in vesicular
    trafficking,activator of PIP5K1A

         Gene Ontology (GO): Selected molecular function terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003924GTPase activity TAS1993656
    GO:0004871signal transducer activity ----
    GO:0005515protein binding IPI16148947
    GO:0005525GTP binding TAS10913182
    GO:0019001guanyl nucleotide binding ----
         
    ARF6 for ontologies           About GeneDecksing


    Phenotypes:
         2 GenomeRNAi human phenotypes for ARF6:
     Increased Salmonella enterica   Increased Salmonella enterica  

         5 MGI mutant phenotypes (inferred from 1 allele(MGI details for Arf6):
     cardiovascular system  hematopoietic system  homeostasis/metabolism  liver/biliary system  mortality/aging 

    ARF6 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Arf6tm1Yaka for ARF6

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ARF6
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ARF6

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for ARF6
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for ARF6

    miRNA
    Products:
        
    miRTarBase miRNAs that target ARF6:
    hsa-mir-128-3p (MIRT021955), hsa-mir-132-3p (MIRT021729), hsa-mir-30a-5p (MIRT028421), hsa-mir-34a-5p (MIRT025265), hsa-mir-103a-3p (MIRT026984), hsa-mir-181a-5p (MIRT025065)

    Block miRNA regulation of human, mouse, rat ARF6 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate ARF6 (see all 33):
    hsa-miR-124* hsa-miR-323-3p hsa-miR-520f hsa-miR-139-5p hsa-miR-218 hsa-miR-500a hsa-miR-3161 hsa-miR-9
    SwitchGear 3'UTR luciferase reporter plasmidARF6 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for ARF6
    Predesigned siRNA for gene silencing in human, mouse, rat ARF6

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for ARF6

    Clone
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    OriGene clones in human, mouse for ARF6 (see all 7)
    OriGene ORF clones in mouse, rat for ARF6
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: ARF6 (NM_001663)
    Sino Biological Human cDNA Clone for ARF6
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ARF6
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ARF6

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for ARF6 
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ARF6


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    ARF6_HUMAN, P62330: Golgi apparatus. Cell membrane; Lipid-anchor. Endosome membrane; Lipid-anchor. Recycling
    endosome membrane; Lipid-anchor (Probable). Cell projection, filopodium membrane; Lipid-anchor. Midbody (By
    similarity). Cytoplasm (By similarity). Cleavage furrow (By similarity). Note=Distributed throughout the
    cytoplasm during metaphase. Transiently detected at the ingressing cleavage furrow during mitotic cytokinesis.
    Recruited to the midbody at later stages of cytokinesis; this requires interaction with KIF23 (By similarity).
    Recruited to the cell membrane in association with CYTH2 and ARL4C. Colocalizes with DAB2IP at the plasma
    membrane and endocytic vesicles
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    endosome5
    golgi apparatus5
    plasma membrane5
    cytosol3
    cytoskeleton2
    vacuole2
    endoplasmic reticulum1
    extracellular1
    lysosome1
    mitochondrion1

    Gene Ontology (GO): Selected cellular component terms (see all 17):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001726ruffle IDA9312003
    GO:0005622intracellular ----
    GO:0005634nucleus ----
    GO:0005737cytoplasm ----
    GO:0005768endosome TAS10913182

    ARF6 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for ARF6 About   (see all 19)  
    See pathways by source

    SuperPathContained pathways About
    1Paxillin-dependent events mediated by a4b1
    Paxillin-dependent events mediated by a4b10.58
    Paxillin-independent events mediated by a4b1 and a4b70.58
    2GPCR Pathway
    Paxillin Interactions0.73
    3Neuropathic Pain-Signaling in Dorsal Horn Neurons
    Cholera Infection0.40
    4Ras signaling pathway
    Ras signaling pathway0.36
    5RhoGDI Pathway
    Fc-GammaR-Mediated Phagocytosis in Macrophages0.33

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    4 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for ARF6
        Cholera Infection
    Paxillin Interactions
    Remodeling of Adherens Junctions
    Fc-GammaR-Mediated Phagocytosis in Macrophages

    2 Cell Signaling Technology (CST) Pathways for ARF6
        Cytoskeletal Signaling
    Protein Stability

    1 GeneGo (Thomson Reuters) Pathway for ARF6
        Immune response CD16 signaling in NK cells

    Selected BioSystems Pathways for ARF6 (see all 10)
        Insulin Signaling
    Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
    Arf6 signaling events
    Arf6 trafficking events
    E-cadherin signaling in the nascent adherens junction



    3 Kegg Pathways  (Kegg details for ARF6):
        Ras signaling pathway
    Endocytosis
    Fc gamma R-mediated phagocytosis


    ARF6 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including ARF6: 
              Adherens Junctions in human mouse rat
              Cell Motility in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for ARF6

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for ARF6 (P623301, 2, 3 ENSP000002983164) via UniProtKB, MINT, STRING, and/or I2D (see all 238)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SPAG9O602711, 2, 3, ENSP000002620134EBI-638181,EBI-1023301 MINT-7922407 MINT-7922423 MINT-7922498 MINT-7922449 MINT-7922462 MINT-7922481 MINT-7922515 MINT-7915283 I2D: score=3 STRING: ENSP00000262013
    KIF23Q022411, 2EBI-638181,EBI-306852 MINT-8397642 MINT-8398021 MINT-8397725 MINT-8397742 MINT-8397810 MINT-8397759 MINT-8397988 MINT-8397913 MINT-8397949 MINT-8395820 MINT-8397844 MINT-8397776 MINT-8398039 MINT-8397691 MINT-8397793 MINT-8397969 MINT-8397669 MINT-8397827 MINT-8398059 MINT-8397708 MINT-8398005 MINT-8397899 MINT-8400598
    ARFIP2P533651, 3, ENSP000002545844EBI-638181,EBI-638194 I2D: score=4 STRING: ENSP00000254584
    ATP6V1C1P212831, 3, ENSP000003792034EBI-638181,EBI-988663 I2D: score=3 STRING: ENSP00000379203
    ENSG00000230685O002993, ENSP000004080944I2D: score=1 STRING: ENSP00000408094
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 25):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001889liver development IEA--
    GO:0006184GTP catabolic process TAS1993656
    GO:0006886intracellular protein transport ----
    GO:0006915apoptotic process ----
    GO:0006928cellular component movement TAS10036235

    ARF6 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ARF6

    1 HMDB Compound for ARF6    About this table
    CompoundSynonyms CAS #PubMed Ids
    Guanosine triphosphate5'-GTP (see all 10)86-01-1--

    3 DrugBank Compounds for ARF6    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    5'-Guanosine-Diphosphate-Monothiophosphate-- --target--17139284 17016423 10592235
    Guanosine-5'-Diphosphate-- 146-91-8target--17139284 17016423 10592235
    MYRISTIC ACID-- --target--10592235

    Selected Novoseek inferred chemical compound relationships for ARF6 gene (see all 28)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    grp-1 86.7 18 10480924 (6), 9832437 (1), 17409355 (1), 18042453 (1)
    gtp 77.1 68 10480924 (3), 9314528 (3), 10391955 (3), 16737952 (3) (see all 31)
    ac-ap 71.5 1 16280360 (1)
    gdp 68.6 22 16527809 (3), 15126638 (2), 11266366 (2), 10480924 (1) (see all 13)
    phosphatidylinositol-3,4,5-trisphosphate 58.5 2 10848997 (1), 10917667 (1)
    adp 56.9 23 12810696 (2), 11463641 (2), 15778454 (1), 18764822 (1) (see all 16)
    pip2 51.5 7 11535619 (3), 16413288 (2), 15817676 (1)
    phosphatidylinositol 51 13 10788515 (2), 17284522 (2), 11950936 (1), 16737952 (1) (see all 8)
    butanol 42 2 11516649 (1), 16280360 (1)
    ptdins(3)p 41.8 1 16413288 (1)



    ARF6 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    2 fRNAdb Secondary structures:


    REFSEQ mRNAs for ARF6 gene: 
    NM_001663.3  

    Unigene Cluster for ARF6:

    ADP-ribosylation factor 6
    Hs.525330  [show with all ESTs]
    Unigene Representative Sequence: NM_001663
    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000298316(uc001wxg.4)
    miRNA
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    Selected qRT-PCR Assays for microRNAs that regulate ARF6 (see all 33):
    hsa-miR-124* hsa-miR-323-3p hsa-miR-520f hsa-miR-139-5p hsa-miR-218 hsa-miR-500a hsa-miR-3161 hsa-miR-9
    SwitchGear 3'UTR luciferase reporter plasmidARF6 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat ARF6
    Clone
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    GenScript: all cDNA clones in your preferred vector: ARF6 (NM_001663)
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ARF6
    Primer
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    OriGene qPCR primer pairs and template standards for ARF6
    OriGene qSTAR qPCR primer pairs in human, mouse for ARF6
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ARF6
      QuantiTect SYBR Green Assays in human, mouse, rat ARF6
      QuantiFast Probe-based Assays in human, mouse, rat ARF6

    Additional mRNA sequence: 

    AF047432.1 AF493885.1 AK313790.1 AL117621.1 BC002952.1 BC008918.2 BC030291.1 BT019868.1 
    CR541939.1 CR541964.1 M57763.1 

    11 DOTS entries:

    DT.455400  DT.86609000  DT.100036242  DT.91769001  DT.100796741  DT.120773507  DT.91768995  DT.95190179 
    DT.100776347  DT.102824727  DT.97855842 

    Selected AceView cDNA sequences (see all 422):

    BG913708 BI091938 BM744596 AI332406 BE614131 C02006 CF457008 AA243143 
    BU661396 AA150941 AA505121 BE882032 BG831535 AA098905 BM541735 BQ315707 
    BE884071 CB111758 BM838197 BI962741 AW371856 CB163386 T32075 AW571594 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    ARF6 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CAGACTATGT
    ARF6 Expression
    About this image


    ARF6 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 7) fully expand
     
     Brain (Nervous System)    fully expand to see all 5 entries
             Cerebral Cortex
     
     Epithelial Cells
             Hepatoblasts Liver Bud
     
     Liver (Hepatobiliary System)
             Hepatoblasts Liver Bud
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Colon (Gastrointestinal Tract)
    ARF6 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ARF6 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.525330

    UniProtKB/Swiss-Prot: ARF6_HUMAN, P62330
    Tissue specificity: Ubiquitous, with higher levels in heart, substantia nigra, and kidney

        Pathway & Disease-focused RT2 Profiler PCR Arrays including ARF6: 
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals and fungi.

    Orthologs for ARF6 gene from Selected species (see all 23)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Arf61 , 5 ADP-ribosylation factor 61, 5 96(n)1
    100(a)1
      12 (28.81 cM)5
    118451  NM_007481.31  NP_031507.11 
     693721505 
    chicken
    (Gallus gallus)
    Aves ARF61 ADP-ribosylation factor 6 88.76(n)
    99.43(a)
      428927  NM_001081705.1  NP_001075174.1 
    lizard
    (Anolis carolinensis)
    Reptilia ARF66
    ADP-ribosylation factor 6
    99(a)
    1 ↔ 1
    GL343627.1(322866-323393)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.9632 Xenopus laevis Arf6 mRNA, complete cds 83.55(n)    U31469.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wufj46d092 wufj46d09 81.23(n)   336063  BC064293.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Arf51F1 , 3 protein amino acid ADP-ribosylation GTP
    binding3
    ADP ribosylation factor 51F1
    96(a)3
    76.63(n)1
    97.13(a)1
      2 51F63
    366991  NM_166091.11  NP_725455.11 
    worm
    (Caenorhabditis elegans)
    Secernentea arf-61 , 3 arf-61 88(a)
    (best of 2)3
    73.95(n)1
    88.51(a)1
      IV(16947228-16949812)3
    1916081  NM_070610.41  NP_503011.11 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes ARF3(YOR094W)4
    ARF31
    Glucose-repressible ADP-ribosylation factor, GTPase more4
    ARF31
    56.98(n)1
    60.47(a)1
      15(502795-503346)4
    8542611, 4  NP_014737.11, 4 


    ENSEMBL Gene Tree for ARF6 (if available)
    TreeFam Gene Tree for ARF6 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for ARF6 gene
    ARL22  ARL5B2  ENSG000002730032  ARL5C2  ARF42  ENSG000002728222  ARF52  ARF12  
    ARL32  ARL5A2  ARF32  TRIM232  
    Selected SIMAP similar genes for ARF6 using alignment to 2 protein entries:     ARF6_HUMAN (see all proteins) (see all similar genes):
    ARF5    ARF1    ARF4    ARF3    ARL17A    ARL17B
    TRIM23    ARL1    ARL14    DKFZp434B2017    ARL2    ARL5A
    ARL4C    ARL7    ARL3    ARL5B    ARL5C    SAR1B

    ARF6 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for ARF6
    PGOHUM00000232934


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for ARF6 (see all 157)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 14 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1408199001,2
    --50357762(+) CCTGGA/GCAACA 1 -- us2k10--------
    rs31009091,2
    C,F,A--50357912(-) aagtgT/Atctgc 1 -- us2k1 tfbs37Minor allele frequency- A:0.13NA WA CSA 131
    rs1928569741,2
    --50357941(+) CCAGTC/GTGGCC 1 -- us2k10--------
    rs1501309701,2
    --50357992(+) GTCAGA/GCGTGG 1 -- us2k10--------
    rs1158923181,2
    C,F--50358101(+) GCACTG/CCAGCC 1 -- us2k11Minor allele frequency- C:0.04WA 118
    rs1834946011,2
    --50358408(+) AAAGAC/TACCCA 1 -- us2k10--------
    rs1887852571,2
    --50358411(+) GATACC/TCATTT 1 -- us2k10--------
    rs1928550081,2
    --50358528(+) ACGCCC/TGTAAT 1 -- us2k10--------
    rs1128589961,2
    C,F--50358544(+) CATTTC/TGGGGG 1 -- us2k11Minor allele frequency- T:0.50WA 2
    rs1493108501,2
    --50358700(+) ACTGCC/TTGAAC 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for ARF6 (50359736 - 50363772 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for ARF6:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv28926CNV Gain+Loss19812545

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing ARF6
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 600464OMIM information:   
    17 diseases for ARF6:    
    About MalaCards
    familial infantile myoclonic epilepsy    cholera    hemophagocytic lymphohistiocytosis    herpes simplex
    hiv-1    breast cancer    lung cancer    cervicitis
    esophagitis    endotheliitis    prostate cancer    prostatitis
    malaria    cerebritis    melanoma    neuronitis
    pancreatitis

    1 disease from the University of Copenhagen DISEASES database for ARF6:
    Cholera

    ARF6 for disorders           About GeneDecksing

    6 Novoseek inferred disease relationships for ARF6 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    glioblastoma 28 5 19642173 (2), 11226253 (1)
    cholera 19.5 3 11106366 (1), 1447192 (1), 16552056 (1)
    invasive cancer 7.62 5 15087504 (2), 16636290 (1)
    tumors 5.14 14 16413265 (5), 19896381 (4), 15210957 (2), 18094045 (1)
    breast cancer 0 20 15087504 (4), 18084281 (4), 16636290 (1), 19416474 (1) (see all 6)
    cancer 0 2 14607973 (2)


    Export disorders for ARF6 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for ARF6 gene, integrated from 10 sources (see all 327):
    (articles sorted by number of sources associating them with ARF6)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular identification of ADP-ribosylation factor mRNAs and their expression in mammalian cells. (PubMed id 1993656)1, 2, 3 Tsuchiya M.... Vaughan M. (J. Biol. Chem. 1991)
    2. Sequence, genomic organization, and expression of the human ADP- ribosylation factor 6 (ARF6) gene: a class III ARF. (PubMed id 14659046)1, 2, 9 Lebeda R.A.... Haun R.S. (DNA Cell Biol. 2003)
    3. Proteomic identification and functional characterization of a novel ARF6 GTPase-activating protein, ACAP4. (PubMed id 16737952)1, 2, 9 Fang Z....Yao X. (amp 2006)
    4. Rab11-FIP3 and FIP4 interact with Arf6 and the exocyst to control membrane traffic in cytokinesis. (PubMed id 16148947)1, 2, 9 Fielding A.B.... Gould G.W. (EMBO J. 2005)
    5. The TBC (Tre-2/Bub2/Cdc16) domain protein TRE17 regulates plasma membrane-endosomal trafficking through activation of Arf6. (PubMed id 15509780)1, 2, 9 Martinu L.... Chou M.M. (Mol. Cell. Biol. 2004)
    6. Myristoylation is required for the intracellular localization and endocytic function of ARF6. (PubMed id 7589240)1, 2, 9 D'Souza-Schorey C. and Stahl P.D. (Exp. Cell Res. 1995)
    7. Molecular characterization of Rab11-FIP3 binding to ARF GTPases. (PubMed id 17628206)1, 2, 9 Schonteich E.... Prekeris R. (Eur. J. Cell Biol. 2007)
    8. The structural basis of Arf effector specificity: the crystal structure of ARF6 in a complex with JIP4. (PubMed id 19644450)1, 2, 9 Isabet T....MAcnActrey J. (EMBO J. 2009)
    9. The Arl4 family of small G proteins can recruit the cytohesin Arf6 exchange factors to the plasma membrane. (PubMed id 17398095)1, 2, 9 Hofmann I.... Munro S. (Curr. Biol. 2007)
    10. The structural GDP/GTP cycle of human Arf6. (PubMed id 11266366)1, 2, 9 Pasqualato S.... Cherfils J. (EMBO Rep. 2001)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 382 HGNC: 659 AceView: ARF6 Ensembl:ENSG00000165527 euGenes: HUgn382
    ECgene: ARF6 Kegg: 382 H-InvDB: ARF6

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for ARF6 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for ARF6 gene:
    Search GeneIP for patents involving ARF6

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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