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Aliases & Descriptions for ARF4
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| | | Descriptions |
|---|
| ADP-ribosylation factor 2 1, 2 | | ADP-ribosylation factor 4 2 |
|
| | Search outside databases for aliases for ARF4 genePrevious GC identifers: GC03M056428 GC03M056911 GC03M057411 GC03M057514 GC03M057507 |
Summaries for ARF4(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for ARF4: This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq] UniProtKB/Swiss-Prot: ARF4_HUMAN, P18085Function: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatusGene Wiki entry for ARF4 |
Genomic Location for ARF4
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the ARF4 gene 
Entrez Gene cytogenetic band: 3p21.2-p21.1 Ensembl cytogenetic band: 3p14.3 HGNC cytogenetic band: 3p21.2-p21.1ARF4 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 3 GeneLoc Exon Structure GeneLoc location for GC03M057532:
(about GC identifiers)
Start:
|
57,532,130 bp from pter |
End:
|
57,558,118 bp from pter |
Size:
|
25,989 bases |
Orientation:
|
minus strand |
RefSeq DNA sequence:- NC_000003.10 NT_022517.17
| Proteins for ARF4
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: ARF4_HUMAN, P18085 (See
protein sequence)Recommended Name: ADP-ribosylation factor 4 Size: 180 amino acids; 20511 Da
Subcellular location: Golgi apparatus
Caution: Was originally thought to be ARF2
PDB structures from and Proteopedia :1Z6X (3D)
 
Secondary accessions: B2R7J7 P21371REFSEQ proteins: NP_001651.1
ENSEMBL proteins: ENSP00000306010
Human Recombinant Proteins               OriGene Purified Recombinant Human Protein: ARF4 
2 Gene Ontology (GO) cellular component terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005622 | intracellular |
IEA | -- | | GO:0005794 | Golgi apparatus |
IEA | -- | About this table
Antibodies for ARF4: Assays for ARF4: | Protein
Domains/ Families for ARF4(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry P18085
ProtoNet protein and cluster: P18085 1 Blocks protein family: IPB006688 ADP-ribosylation factor
UniProtKB/Swiss-Prot: ARF4_HUMAN, P18085Similarity: Belongs to the small GTPase superfamily. Arf family | Gene Function for ARF4
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001660
Applied Biosystems Silencer® siRNAs for ARF4
Sigma-Aldrich siRNA for ARF4  Sigma-Aldrich shRNA for ARF4  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001660                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_001660                                  untagged cDNA clone in CMV expression vector: NM_001660 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_001660
UniProtKB/Swiss-Prot: ARF4_HUMAN, P18085Function: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatusGenatlas biochemistry entry for ARF4:ADP ribosylation factor 4,class II,20kDa,low molecular weight GTP binding protein,a guanine nucleotide dependent protein activator of cholera toxin,widely expressed,except in brain,involved in vesicular trafficking and activation of phospholipase D3 Gene Ontology (GO) molecular function terms (links to tree view): About this table | Pathways & Interactions for ARF4
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
| 1 Sigma-Aldrich "Your Favorite Gene" Pathway for ARF4 (Your Favorite Gene powered by Ingenuity) 
Gene Network CentralTM Interacting Genes and Proteins Network for ARF4 
5/13 Interacting proteins for ARF4 (ENSP000003060103) via UniProtKB, MINT, and/or STRING (see all 13
)About this table
3 Gene Ontology (GO) biological process terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0007264 | small GTPase mediated signal transduction |
IEA | -- | | GO:0015031 | protein transport |
IEA | -- | | GO:0016192 | vesicle-mediated transport |
IEA | -- | About this table
|
Drugs & Compounds for ARF4(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|  |
Enzo Life Sciences drugs & compounds for ARF4 |
Browse Tocris compounds for ARF4 2 PharmGKB drug compound relationships for ARF4 geneAbout this table
|
Transcripts for ARF4(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001660
Sigma-Aldrich siRNA for ARF4  Sigma-Aldrich shRNA for ARF4  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_001660 REFSEQ mRNAs for ARF4 gene: NM_001660.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_001660               OriGene GFP tagged cDNA clone in CMV expression vector: NM_001660                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_001660                                  untagged cDNA clone in CMV expression vector: NM_001660  Additional cDNA sequence: AF493883.1 AK313008.1 BC003364.1 BC008753.2 BC016325.1 BC022866.1 CR591127.1 CR591617.1 CR592933.1 CR595935.1 CR596585.1 CR597516.1 CR607207.1 CR607867.1 CR611196.1 CR611234.1 CR615713.1 CR618819.1 CR622582.1 CR625816.1 CR625897.1 M36341.1 24/31 DOTS entries (see all 31
): DT.120191 DT.92465044 DT.95278541 DT.92389272 DT.100883817 DT.92039146 DT.100883824 DT.100678387 DT.120912848 DT.120912819 DT.100883821 DT.120912851 DT.100032418 DT.92465093 DT.100883809 DT.100883810 DT.100883812 DT.92377942 DT.92465055 DT.100039128 DT.120912903 DT.120912924 DT.121494332 DT.95322144 24/1234 AceView cDNA sequences (see all 1234
):CR607207 AA385193 BX391951 BU679960 R58940 CR591127 BU683333 AA330315 T27653 AL555133 BC003364 BU155868 BF593865 AW262738 AA248202 BM802529 BU632851 CB158141 BE897537 W25530 CB145784 AV722290 CB055283 AA131305
highest scoring ESTs for ARF4:M36341 AA058455 AA081519 AA133436 AA161518 AA205553 AA296296 AA304113 AA305135 AA307503 Unigene Cluster for ARF4: ADP-ribosylation factor 4 Hs.695943 [show with all ESTs]Unigene Representative Sequence: NM_001660
GeneLoc Exon Structure
5/7 Alternative Splicing Database (ASD) splice patterns (SP) for ARF4 (see all 7
)
| ExUns: | 1a | · | 1b | · | 1c | · | 1d | · | 1e | ^ | 2a | · | 2b | ^ | 3 | ^ | 4 | ^ | 5a | · | 5b | · | 5c | · | 5d | ^ | 6 | ^ | 7a | · | 7b | ^ | 8a | · | 8b | · | 8c | · | 8d | |
| SP1: | |   | |   | |   | |   | |   | |   | |   | - |   | |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP2: | |   | |   | |   | |   | |   | |   | |   | - |   | |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP3: | |   | |   | |   | |   | |   | |   | |   | - |   | |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP4: | |   | |   | |   | |   | |   | |   | |   | - |   | |   | - |   | - |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |
| SP5: | |   | |   | |   | |   | |   | |   | |   | - |   | - |   | - |   | - |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   |
About this scheme
ECgene alternative splicing isoforms for ARF4
1 Ensembl transcript including schematic representation: ENST00000303436
|
Expression for ARF4
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| ARF4 expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for ARF4
1 / 2 / 3 5 probe-sets matching ARF4 gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: TTGCTTTTGT
SOURCE GeneReport for Unigene cluster: Hs.695943
Expression variation in blood from EXPOLDB for ARF4 |
Orthologs for ARF4
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for ARF4 gene from 5/11 species (see all 11
)
About this table Species with no ortholog for ARF4
ENSEMBL Gene Tree for ARF4 | Paralogs for ARF4(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Paralogs for ARF4 gene
- ARF52
Pseudogenes
- 4 related pseudogenes
|
SNPs/Variants for ARF4(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for ARF4 (up to first 250kb)
|
Disorders & Mutations for ARF4
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 601177
1 Novoseek disease relationship for ARF4 gene
| Disease |
Score |
Articles |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| cholera |
38.16 |
2 |
10524252 (1) |
About this table
1 PharmGKB disease relationship for ARF4 geneAbout this table
|
Medical News for ARF4(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for ARF4 (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/30 PubMed articles for ARF4 gene (see all 30
):- Selective amplification of an mRNA and related pseudogene for a human ADP-ribosylation factor, a guanine nucleotide-dependent protein activator of cholera toxin. (PubMed id 2107548)2, 3, 4 Monaco L....Vaughan M. (1990)
- Cloning and characterization of the human ADP-ribosylation factor 4 gene. (PubMed id 10524252)1, 3, 4 Lebeda R.A. and Haun R.S. (1999)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)3, 4 Gerhard D.S....Malek J. (2004)
- Human ADP-ribosylation factors. A functionally conserved family of GTP-binding proteins. (PubMed id 1899243)3, 4 Kahn R.A.... Rulka C. (1991)
- Identification of ADP-ribosylation factor 4 as a suppressor of N-(4-hydroxyphenyl)retinamide-induced cell death. (PubMed id 19041174)1, 3 Woo I.S....Seo H.G. (2009)
- Human ADP-ribosylation factor 4 (ARF4) gene. Map position 3p14.1. (PubMed id 10099884)1, 3 Kim H.S. (1998)
- Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)3 Kimura K.... Sugano S. (2006)
- Role of ARF4L in recycling between endosomes and the plasma membrane. (PubMed id 15049518)3 Katayama T....Tohyama M. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)4 Ota T....Sugano S. (2004)
- Treatment-specific changes in gene expression discriminate in vivo drug response in human leukemia cells. (PubMed id 12704389)7 Cheok M.H....Evans W.E. (2003)
|
Search for ARF4
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing ARF4
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing ARF4
(According to HUGE)
About This Section
| -- |
Specialized Databases showing ARF4(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for ARF4(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for ARF4:

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Incremental update: 13 Oct 2009
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