Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



ARAF Gene

protein-coding   GIFtS: 66
GCID: GC0XP047429

V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog

(Previous name: v-raf murine sarcoma 3611 viral oncogene homolog 1)
(Previous symbol: ARAF1)
  See ARAF-related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog1 2     A-RAF2
ARAF11 2 3 5     A-Raf Proto-Oncogene Serine/Threonine-Protein Kinase2
PKS22 3 5     Oncogene ARAF12
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog 11 2     Ras-Binding Protein DA-Raf2
Proto-Oncogene A-Raf-12 3     Serine/Threonine-Protein Kinase A-Raf2
Proto-Oncogene Pks2 3     PKS3
EC 2.7.11.13 8     Proto-Oncogene A-Raf3
RAFA12 5     EC 2.7.118

External Ids:    HGNC: 6461   Entrez Gene: 3692   Ensembl: ENSG000000780617   OMIM: 3110105   UniProtKB: P103983   

Export aliases for ARAF gene to outside databases

Previous GC identifers: GC0XP047177 GC0XP047305 GC0XP045132


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for ARAF Gene:
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell
growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for
this gene.(provided by RefSeq, Jan 2012)

GeneCards Summary for ARAF Gene:
ARAF (v-raf murine sarcoma 3611 viral oncogene homolog) is a protein-coding gene. Diseases associated with ARAF include mycobacterium marinum. GO annotations related to this gene include protein serine/threonine kinase activity and MAP kinase kinase kinase activity. An important paralog of this gene is FPGT-TNNI3K.

UniProtKB/Swiss-Prot: ARAF_HUMAN, P10398
Function: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also
regulate the TOR signaling cascade
Function: Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the
expression of myogenin and other muscle-specific proteins, and myotube formation

summary for ARAF Gene:
Raf kinases, a family of three serine/threonine kinases, are part of the ras-MAPK signaling cascade and
phosphorylate MEK. Upon growth factor stimulation, Raf-1 (or c-Raf) is activated by GTP-bound Ras and
recruited to the cell membrane. This activation process is tightly regulated by a number of factors
including phosphatases (e.g. PP1, PP2A, PP5), kinases (e.g. Src, ERK, Akt, PKC) and proteins that bind
directly to Raf-1 (e.g. RKIP, 14-3-3zeta, KSR, Hsp90). Raf-1 is also thought to be able to dimerize with
wild type B-Raf in a Ras-dependent process. B-raf is commonly mutated and thereby activated in many human
cancers, the most frequent mutation being the V600E mutation of the kinase domain. Whilst wt b-Raf and Raf-1
are strongly activated by growth factor signals via Ras and Src, a-Raf is only modestly activated and has
low basal activity. All three isoforms of Raf are considered to be oncogenic.

Gene Wiki entry for ARAF Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence:
NC_000023.11  NT_079573.5  NC_018934.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the ARAF gene promoter:
         Elk-1   C/EBPbeta   AP-1   ATF-2   CREB   deltaCREB   Sox9   c-Jun   NF-kappaB1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidARAF promoter sequence
   Search Chromatin IP Primers for ARAF

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ARAF


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xp11.4-p11.2   Ensembl cytogenetic band:  Xp11.23   HGNC cytogenetic band: Xp11.3-p11.23

ARAF Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ARAF gene location

GeneLoc information about chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XP047429:  view genomic region     (about GC identifiers)

Start:
47,420,516 bp from pter      End:
47,431,320 bp from pter
Size:
10,805 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: ARAF_HUMAN, P10398 (See protein sequence)
Recommended Name: Serine/threonine-protein kinase A-Raf  
Size: 606 amino acids; 67585 Da
Cofactor: Binds 2 zinc ions per subunit (By similarity)
Subunit: Interacts with TH1L/NELFD
1 PDB 3D structure from and Proteopedia for ARAF:
1WXM (3D)    
Secondary accessions: P07557 Q5H9B2 Q5H9B3
Alternative splicing: 2 isoforms:  P10398-1   P10398-2   (Has a wider tissue distribution than isoform 1, and acts as dominant-negative antagonist)

Explore the universe of human proteins at neXtProt for ARAF: NX_P10398

Explore proteomics data for ARAF at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for ARAF (P10398) (see all 16)
     NILLFMG  GSVLWMA  FHQHCSS  RKTFFSL 


    See ARAF Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_001243125.1  NP_001243126.1  NP_001645.1  

    ENSEMBL proteins: 
     ENSP00000366244   ENSP00000366238   ENSP00000290277  

    ARAF Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for ARAF
    Browse OriGene Protein Over-expression Lysates
    OriGene MassSpec for ARAF
    OriGene Custom Protein Services for ARAF
    GenScript Custom Purified and Recombinant Proteins Services for ARAF
    Novus Biologicals ARAF Proteins
    Novus Biologicals ARAF Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for ARAF

     
    Search eBioscience for Proteins for ARAF 

    ARAF Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    R&D Systems Antibodies for ARAF (A-Raf)
    Cell Signaling Technology (CST) Antibodies for ARAF 
    OriGene Antibodies for ARAF
    OriGene Custom Antibody Services for ARAF
    Novus Biologicals ARAF Antibodies
    Search for Antibodies for ARAF at Abcam
    Cloud-Clone Corp. Antibodies for ARAF
    ThermoFisher Antibody for ARAF
    LSBio Antibodies in human, mouse, rat for ARAF

    ARAF Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for ARAF
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for ARAF
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for ARAF
    Cloud-Clone Corp. CLIAs for ARAF
    Search eBioscience for ELISAs for ARAF 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    IUPHAR Guide to PHARMACOLOGY protein family classification: v-raf murine sarcoma 3611 viral oncogene homolog
    RAF family

    Selected InterPro protein domains (see all 8):
     IPR017441 Protein_kinase_ATP_BS
     IPR020454 DAG/PE-bd
     IPR011009 Kinase-like_dom
     IPR008271 Ser/Thr_kinase_AS
     IPR000719 Prot_kinase_dom

    Graphical View of Domain Structure for InterPro Entry P10398

    ProtoNet protein and cluster: P10398

    2 Blocks protein domains:
    IPB002219 Protein kinase C
    IPB003116 Raf-like Ras-binding


    UniProtKB/Swiss-Prot: ARAF_HUMAN, P10398
    Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily
    Similarity: Contains 1 phorbol-ester/DAG-type zinc finger
    Similarity: Contains 1 protein kinase domain
    Similarity: Contains 1 RBD (Ras-binding) domain


    Find genes that share domains with ARAF           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ARAF_HUMAN, P10398
    Function: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also
    regulate the TOR signaling cascade
    Function: Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the
    expression of myogenin and other muscle-specific proteins, and myotube formation
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein

         Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112

         Gene Ontology (GO): Selected molecular function terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity TAS3029685
    GO:0004674protein serine/threonine kinase activity IDA19667065
    GO:0004709MAP kinase kinase kinase activity ----
    GO:0004713protein tyrosine kinase activity ----
    GO:0005057receptor signaling protein activity IEA--
         
    Find genes that share ontologies with ARAF           About GenesLikeMe


    Phenotypes:
         7 GenomeRNAi human phenotypes for ARAF:
     Decreased focal adhesion (FA)   Decreased substrate adherent c  G0/1 arrest  Increased G1 DNA content 
     Increased cell death HMECs cel  Increased viability with 4OH t  Synthetic lethal with Ras 

         7 MGI mutant phenotypes (inferred from 1 allele(MGI details for Araf):
     behavior/neurological  digestive/alimentary  growth/size/body  hematopoietic system  immune system 
     mortality/aging  muscle 

    Find genes that share phenotypes with ARAF           About GenesLikeMe

    Animal Models:
         MGI mouse knock-out Araftm1Mmc for ARAF

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ARAF
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ARAF

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for ARAF
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for ARAF

    miRNA
    Products:
        
    miRTarBase miRNAs that target ARAF:
    hsa-mir-331-3p (MIRT043358), hsa-mir-124-3p (MIRT004929)

    Block miRNA regulation of human, mouse, rat ARAF using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate ARAF (see all 20):
    hsa-miR-218 hsa-miR-3190 hsa-miR-520a-5p hsa-miR-124 hsa-miR-342-5p hsa-miR-199b-5p hsa-miR-506 hsa-miR-28-5p
    SwitchGear 3'UTR luciferase reporter plasmidARAF 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for ARAF
    Predesigned siRNA for gene silencing in human, mouse, rat ARAF

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for ARAF

    Clone
    Products:
         
    OriGene clones in human, mouse for ARAF (see all 9)
    OriGene ORF clones in mouse, rat for ARAF
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: ARAF (NM_001654)
    Sino Biological Human cDNA Clone for ARAF
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ARAF
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ARAF
    Addgene plasmids for ARAF 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for ARAF
    Browse ESI BIO Cell Lines and PureStem Progenitors for ARAF 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ARAF


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol3
    mitochondrion2
    nucleus2
    extracellular1
    plasma membrane1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005575cellular_component ND--
    GO:0005739mitochondrion IEA--
    GO:0005829cytosol ----

    Find genes that share ontologies with ARAF           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for ARAF About   (see all 32)  
    See pathways by source

    SuperPathContained pathways About
    1Glioma
    Glioma0.51
    Prostate cancer0.39
    Non-small cell lung cancer0.51
    Signaling Pathways in Glioblastoma0.36
    Melanoma0.43
    Bladder cancer0.31
    2ErbB signaling pathway
    ErbB signaling pathway0.52
    ErbB signaling pathway0.52
    3Sorafenib Pharmacodynamics
    Vemurafenib Pathway, Pharmacodynamics0.37
    VEGF Pathway0.36
    4IL-9 Signaling Pathways
    IL-9 Signaling Pathways0.90
    IL-2 Signaling Pathways0.88
    IL-7 Signaling Pathways0.90
    IL-15 Signaling Pathways0.88
    IL-21 Signaling Pathways0.90
    IL-4 Signaling Pathways0.84
    Common Cytokine Receptor Gamma-Chain Family Signaling Pathways0.90
    Adipocytokines and Insulin Signaling0.34
    5Endometrial cancer
    Endometrial cancer0.45
    Acute myeloid leukemia0.40
    Colorectal cancer0.42


    Find genes that share SuperPaths with ARAF           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    Selected R&D Systems Pathways for ARAF (see all 10)
        IL-21 Signaling Pathways
    IL-2 Signaling Pathways
    IL-4 Signaling Pathways
    Adipocytokines & Insulin Signaling
    IL-7 Signaling Pathways

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for ARAF (see all 48)
        FLT3 Signaling
    IL-4 Pathway
    eIF2 Pathway
    TRKA Signaling
    Aldosterone Signaling in Epithelial Cells

    1 Cell Signaling Technology (CST) Pathway for ARAF
        MAP Kinase Signaling

    3 Tocris Bioscience Pathways for ARAF
        Insulin Pathway
    VEGF Pathway
    MAPK Pathway

    3 GeneGo (Thomson Reuters) Pathways for ARAF
        G-protein signaling M-RAS regulation pathway
    G-protein signaling K-RAS regulation pathway
    G-protein signaling N-RAS regulation pathway

    Selected BioSystems Pathways for ARAF (see all 7)
        Focal Adhesion
    Integrin-mediated cell adhesion
    EGFR1 Signaling Pathway
    ErbB signaling pathway
    Integrated Breast Cancer Pathway


    1 PharmGKB Pathway for ARAF
        Vemurafenib Pathway, Pharmacodynamics

    Selected Kegg Pathways  (Kegg details for ARAF) (see all 25):
        ErbB signaling pathway
    FoxO signaling pathway
    Vascular smooth muscle contraction
    Natural killer cell mediated cytotoxicity
    Long-term potentiation

        Pathway & Disease-focused RT2 Profiler PCR Arrays including ARAF: 
              Insulin Signaling Pathway in human mouse rat
              EGF / PDGF Signaling Pathway in human mouse rat
              MAP Kinase (MAPK) Signaling Pathway in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for ARAF

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for ARAF (P103981, 2, 3 ENSP000003662444) via UniProtKB, MINT, STRING, and/or I2D (see all 139)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RABGGTBP536111, 2, 3, ENSP000003174734EBI-365961,EBI-536715 MINT-19316 I2D: score=6 STRING: ENSP00000317473
    NELFCDQ8IXH71, 2, 3, ENSP000003423004EBI-365961,EBI-536725 MINT-19318 I2D: score=5 STRING: ENSP00000342300
    PRPF6O949061, 2, 3, ENSP000002660794EBI-365961,EBI-536755 MINT-19319 I2D: score=5 STRING: ENSP00000266079
    MAP2K2P365071, 3, ENSP000002629484EBI-365961,EBI-1056930 I2D: score=5 STRING: ENSP00000262948
    TIMM44O436151, 3, ENSP000002705384EBI-365961,EBI-861737 I2D: score=5 STRING: ENSP00000270538
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006464cellular protein modification process TAS3029685
    GO:0006468protein phosphorylation ----
    GO:0007165signal transduction ----
    GO:0032006regulation of TOR signaling IMP--
    GO:0032434regulation of proteasomal ubiquitin-dependent protein catabolic process IMP--

    Find genes that share ontologies with ARAF           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
    Selected ApexBio Compounds for ARAF (see all 13)     About this table
    CompoundAction CAS #
    AZ 628selective RAF inhibitor[878739-06-1]
    B-Raf inhibitoruseful in the treatment of cancer and proliferative diseases[1315330-11-0]
    BRAF inhibitora potent BRAF inhibitor[918505-61-0]
    CEP-32496Potent BRAF inhibitor[1188910-76-0]
    Dabrafenib Mesylate (GSK-2118436)selective BRAFV600E inhibitor[1195768-06-9]
    GDC-0879Potent and selective B-Raf inhibitor[905281-76-7]
    PF-04880594RAF inhibitor for BRAF/BRAFV599E and c-RAF[1111636-35-1]
    PLX-4720Potent and selective inhibitor of BRAF with V600E mutation[918505-84-7]
    RAF265Potent and selective Raf inhibitor, also inhibits VEGFR[927880-90-8]
    SorafenibMulti-target kinase inhibitor of Raf kinases and tyrosine kinases[284461-73-0]

    Compounds for ARAF available from Tocris Bioscience    About this table
    CompoundAction CAS #
    GDC 0879Potent B-Raf inhibitor[905281-76-7]
    ZM 336372 Potent, selective c-Raf inhibitor [208260-29-1]
    GW 5074 Potent, selective c-Raf1 kinase inhibitor [220904-83-6]
    SB 590885Potent B-Raf inhibitor[405554-55-4]
    L-779,450 Potent Raf kinase inhibitor[303727-31-3]

    Selected HMDB Compounds for ARAF (see all 845)    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    DG(14:0/14:0/0:0)DAG(14:0/14:0) (see all 9)----
    DG(14:0/14:1(9Z)/0:0)DAG(14:0/14:1) (see all 15)----
    DG(14:0/15:0/0:0)Diacylglycerol(29:0) (see all 9)----
    DG(14:0/16:0/0:0)DAG(30:0) (see all 9)----
    DG(14:0/16:1(9Z)/0:0)DG(30:1) (see all 15)----
    DG(14:0/18:0/0:0)DG(14:0/18:0) (see all 9)----
    DG(14:0/18:1(11Z)/0:0)Diacylglycerol (see all 15)----
    DG(14:0/18:1(9Z)/0:0)1-myristoyl-2-oleoyl-sn-glycerol (see all 15)----

    1 DrugBank Compound for ARAF    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Adenosine triphosphate5'-ATP (see all 8)56-65-5target--17139284 17016423

    Selected Novoseek inferred chemical compound relationships for ARAF gene (see all 38)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    6-deoxyerythronolide b 91.8 17 15885088 (1), 17328673 (1), 8652546 (1), 11939803 (1) (see all 13)
    pikromycin 90.1 10 10713461 (1), 17565713 (1), 18245260 (1), 18512859 (1) (see all 5)
    10-deoxymethynolide 89.2 1 11223265 (1)
    methymycin 86.6 1 17565713 (1)
    mycolactone 85.6 8 18840298 (2), 17031885 (2), 15758215 (1), 19383681 (1)
    narbonolide 84.1 1 11223265 (1)
    n-acetylcysteamine 82.4 1 11439032 (1)
    tetracenomycin 77.1 5 15260498 (4)
    candicidin 75.5 6 12664141 (3), 11782498 (1)
    vibriobactin 72.2 1 15366944 (1)



    Find genes that share compounds with ARAF           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for ARAF gene (3 alternative transcripts): 
    NM_001256196.1  NM_001256197.1  NM_001654.4  

    Unigene Cluster for ARAF:

    V-raf murine sarcoma 3611 viral oncogene homolog
    Hs.446641  [show with all ESTs]
    Unigene Representative Sequence: NM_001256196
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000377045(uc011mln.2 uc011mlo.2 uc004dic.1 uc011mlp.2)
    ENST00000377039 ENST00000489496 ENST00000469505 ENST00000470206 ENST00000290277

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat ARAF using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate ARAF (see all 20):
    hsa-miR-218 hsa-miR-3190 hsa-miR-520a-5p hsa-miR-124 hsa-miR-342-5p hsa-miR-199b-5p hsa-miR-506 hsa-miR-28-5p
    SwitchGear 3'UTR luciferase reporter plasmidARAF 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for ARAF
    Predesigned siRNA for gene silencing in human, mouse, rat ARAF
    Clone
    Products:
         
    OriGene clones in human, mouse for ARAF (see all 9)
    OriGene ORF clones in mouse, rat for ARAF
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: ARAF (NM_001654)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ARAF
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ARAF
    Addgene plasmids for ARAF 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for ARAF
    OriGene qSTAR qPCR primer pairs in human, mouse for ARAF
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ARAF
      QuantiTect SYBR Green Assays in human, mouse, rat ARAF
      QuantiFast Probe-based Assays in human, mouse, rat ARAF

    Additional mRNA sequence: 

    AB158254.1 AF052136.1 AK130043.1 AK297453.1 AK300975.1 AK307747.1 BC002466.2 BC007514.2 
    BT019864.1 M13829.1 X04790.1 

    23 DOTS entries:

    DT.102836980  DT.100812399  DT.100658177  DT.100812408  DT.448494  DT.121285030  DT.100812402  DT.121285068 
    DT.100812404  DT.92058039  DT.121285038  DT.97862004  DT.100812395  DT.95108590  DT.95245717  DT.121285071 
    DT.100812400  DT.121285049  DT.121285078  DT.91640785  DT.91684943  DT.100835282  DT.99996839 

    Selected AceView cDNA sequences (see all 323):

    BG392456 BU180722 CD515482 BP346188 BM759611 AK130043 BX355442 BF931458 
    AW674355 AA332774 CA424869 CA392044 F13363 BC007514 BM713862 BE264900 
    BQ961820 BE378713 X04790 BQ929597 BU733087 BQ880855 AU122046 BM921828 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for ARAF (see all 9)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7a · 7b · 7c ^ 8a · 8b ^ 9a · 9b · 9c · 9d ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^
    SP1:                          -     -                 -     -     -                       -           -     -                                                   
    SP2:                                                  -     -     -                       -           -     -                                                   
    SP3:                          -     -                 -     -     -                       -           -                                                         
    SP4:                          -     -                 -     -     -                       -                                                                     
    SP5:                          -     -                 -           -                                                                                             

    ExUns: 15 ^ 16 ^ 17 ^ 18
    SP1:                        
    SP2:                        
    SP3:                        
    SP4:                        
    SP5:                        


    ECgene alternative splicing isoforms for ARAF

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    ARAF expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TGGATTTTGG
    ARAF Expression
    About this image

    ARAF Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ARAF Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.446641

    UniProtKB/Swiss-Prot: ARAF_HUMAN, P10398
    Tissue specificity: Predominantly in urogenital tissues

        Pathway & Disease-focused RT2 Profiler PCR Arrays including ARAF: 
              Insulin Signaling Pathway in human mouse rat
              EGF / PDGF Signaling Pathway in human mouse rat
              MAP Kinase (MAPK) Signaling Pathway in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for ARAF
    OriGene qSTAR qPCR primer pairs in human, mouse for ARAF
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ARAF
    QuantiTect SYBR Green Assays in human, mouse, rat ARAF
    QuantiFast Probe-based Assays in human, mouse, rat ARAF
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ARAF

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for ARAF gene from Selected species (see all 12)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Araf1 , 5 v-raf murine sarcoma 3611 viral oncogene homolog1, 5 90.29(n)1
    95.53(a)1
      X (16.30 cM)5
    118361  NM_009703.21  NP_033833.11 
     207978145 
    lizard
    (Anolis carolinensis)
    Reptilia ARAF6
    v-raf murine sarcoma 3611 viral oncogene homolog
    63(a)
    1 ↔ 1
    2(68096241-68132063)
    zebrafish
    (Danio rerio)
    Actinopterygii araf1 v-raf murine sarcoma 3611 viral oncogene homolog 65.62(n)
    71.01(a)
      403063  NM_205743.1  NP_991306.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta phl6
    pole hole
    35(a)
    1 → many
    X(2189917-2237903)
    worm
    (Caenorhabditis elegans)
    Secernentea lin-456
    Protein LIN-45, isoform b
    30(a)
    1 → many
    IV(6742538-6748424) WBGene00003030


    ENSEMBL Gene Tree for ARAF (if available)
    TreeFam Gene Tree for ARAF (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for ARAF gene
    FPGT-TNNI3K2  MAP3K122  MAP3K112  KIAA18042  RAF12  KSR22  KSR12  MOS2  
    BRAF2  MAP3K92  MAP3K102  MAP3K72  TNNI3K2  ZAK2  MAP3K132  ILK2  
    4 SIMAP similar genes for ARAF using alignment to 3 protein entries:     ARAF_HUMAN (see all proteins):
    BRAF    RAF1    CDK4    SRGAP3

    Find genes that share paralogs with ARAF           About GenesLikeMe


    3 Pseudogenes.org Pseudogenes for ARAF
    PGOHUM00000232787 PGOHUM00000232806 PGOHUM00000233315


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for ARAF (see all 329)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr X posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0403764
    A colorectal adenocarcinoma sample4--see VAR_0403762 G C mis40--------
    rs1999783541,2
    --47338368(+) GTTGA-/GGCCCC 3 -- us2k10--------
    rs616652401,2
    C--47338369(+) TTGAGC/GCCCCC 3 -- us2k14Minor allele frequency- G:0.17WA NA CSA 6
    rs2010035111,2
    --47338369(+) TGAGG-/CCCCCC 3 -- us2k10--------
    rs732061251,2
    C,F--47338525(+) AATGAG/ATCCTC 3 -- us2k11Minor allele frequency- A:0.00NA 1
    rs1833748291,2
    --47338819(+) GATCCA/GCCCGC 3 -- us2k10--------
    rs1879090301,2
    --47339124(+) AATTGC/TCTTTA 3 -- us2k10--------
    rs1912964081,2
    --47339125(+) ATTGCC/TTTTAT 3 -- us2k10--------
    rs734943991,2
    C--47339151(+) AACTCG/ATATCT 3 -- us2k11Minor allele frequency- A:0.50WA 2
    rs1826629161,2
    --47339163(+) TATGAA/CAAGCA 3 -- us2k10--------

    HapMap Linkage Disequilibrium report for ARAF (47420516 - 47431320 bp)

    Structural Variations
          Database of Genomic Variants (DGV) variations for ARAF: --
    Locus Specific Mutation Databases (LSDB): ARAF

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing ARAF
    DNA2.0 Custom Variant and Variant Library Synthesis for ARAF

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 311010    OMIM disorders: --

    1 disease for ARAF:    
    About MalaCards
    mycobacterium marinum


    Find genes that share disorders with ARAF           About GenesLikeMe

    4 Novoseek inferred disease relationships for ARAF gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    cancer 2.69 2 20145135 (2)
    aids 0 1 11251290 (1)
    ovarian cancer 0 2 16332724 (2)
    tuberculosis 0 1 15042094 (1)


    Export disorders for ARAF gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for ARAF gene, integrated from 10 sources (see all 204):
    (articles sorted by number of sources associating them with ARAF)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1). (PubMed id 8020955)1, 2, 9 Lee J.-E.... Rapp U.R. (Genomics 1994)
    2. Identification of TH1 as an interaction partner of A-Raf kinase. (PubMed id 11952167)1, 2, 9 Yin X.L.... Gu J.X. (Mol. Cell. Biochem. 2002)
    3. DA-Raf1, a competent intrinsic dominant-negative antagonist of the Ras-ERK pathway, is required for myogenic differentiation. (PubMed id 17535970)1, 2 Yokoyama T.... Endo T. (J. Cell Biol. 2007)
    4. A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)1, 2 Beausoleil S.A.... Gygi S.P. (Nat. Biotechnol. 2006)
    5. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)1, 2 Olsen J.V....Mann M. (Cell 2006)
    6. The DNA sequence of the human X chromosome. (PubMed id 15772651)1, 2 Ross M.T.... Bentley D.R. (Nature 2005)
    7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    8. The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus. (PubMed id 3029685)1, 2 Beck T.W.... Rapp U.R. (Nucleic Acids Res. 1987)
    9. Pks, a raf-related sequence in humans. (PubMed id 3529082)1, 2 Mark G.E.... Mountz J.D. (Proc. Natl. Acad. Sci. U.S.A. 1986)
    10. Heterogeneous nuclear ribonucleoprotein H blocks MST2-mediated apoptosis in cancer cells by regulating A-Raf transcription. (PubMed id 20145135)1, 9 Rauch J....Gires O. (Cancer Res. 2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 369 HGNC: 646 AceView: ARAF1 Ensembl:ENSG00000078061 euGenes: HUgn369
    ECgene: ARAF Kegg: 369 H-InvDB: ARAF

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for ARAF Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for ARAF Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for ARAF gene:
    Search GeneIP for patents involving ARAF

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     
     EMD Millipore genomic analysis products

      
     Antibodies for ARAF (A-Raf)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for ARAF   OriGene RNAi products in human, mouse, rat for ARAF  
     OriGene qPCR primer pairs and template standards for ARAF   Browse OriGene Protein Over-expression Lysates  
     OriGene MassSpec something-or-other for ARAF   OriGene clones in human, mouse for ARAF  
     OriGene qSTAR qPCR primer pairs in human, mouse for ARAF   OriGene Purified Protein for ARAF  
     OriGene ORF clones in mouse, rat for ARAF   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for ARAF   OriGene Custom Protein Services for ARAF  

     
     
     Block miRNA regulation of human, mouse, rat ARAF using miScript Target Protectors SeqTarget long-range PCR primers for resequencing ARAF
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ARAF Predesigned siRNA for gene silencing in human, mouse, rat ARAF
     QuantiFast Probe-based Assays in human, mouse, rat ARAF QuantiTect SYBR Green Assays in human, mouse, rat ARAF
     PCR Arrays including human, mouse, rat ARAF Search Chromatin IP Primers for ARAF
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ARAF  GeneGlobe Interaction Network for ARAF
     Regulatory tfbs in ARAF promoter
     GenScript Custom Purified and Recombinant Proteins Services for ARAF GenScript cDNA clones with any tag delivered in your preferred vector for ARAF
     GenScript Custom Assay Services for ARAF GenScript Custom overexpressing Cell Line Services for ARAF
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Antibodies & Assays for ARAF 

     Tocris compounds for ARAF
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     cDNA Clones for ARAF
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     ARAF antibodies
     ARAF proteins
     ARAF lysates
     Search for Antibodies for ARAF at Abcam
     See all of Abcam's Antibodies, Kits and Proteins for ARAF
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for ARAF
     Antibodies for ARAF
     ELISAs for ARAF
     CLIAs for ARAF



     Browse ESI BIO Cell Lines and PureStem Progenitors for ARAF
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ARAF
     SwitchGear 3'UTR luciferase reporter plasmids for ARAF
     SwitchGear Promoter luciferase reporter plasmids for ARAF
     ThermoFisher Antibody for ARAF
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ARAF
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ARAF
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ARAF
     LSBio Antibodies in human, mouse, rat for ARAF
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     ApexBio compounds for ARAF
     Addgene plasmids for ARAF
      Search eBioscience for proteins for ARAF
      Search eBioscience for elisas for ARAF
      eBioscience FlowRNA Probe Sets
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      ARAF gene at Home site.
    Version: 3.12.202 14 Sep 2014
    hostname: 356980-web2.xennexinc.com index build: 128 solr: 1.4