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APAF1 Gene

protein-coding GIFtS: 69

GC12P097541

apoptotic peptidase activating factor 1
(Previous names: apoptotic protease activating factor, apoptotic peptidase activating factor )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
APAF-1 ^¹, ²
Apaf-1 ^³
CED4 ^¹, ²
DKFZp781B1145 ^²
KIAA0413 ^³

Descriptions
apoptotic peptidase activating factor ^¹
apoptotic peptidase activating factor 1 ^²
apoptotic protease activating factor ^¹
apoptotic protease activating factor 1 ^²

External Ids
HGNC: 576¹
Entrez Gene: 317²
UniProtKB: O14727³
Ensembl: ENSG00000120868⁷

Search outside databases for aliases for APAF1 gene

Previous GC identifers: GC12P098489 GC12P099542 GC12P098971

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for APAF1:

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several

copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC).

Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome

binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase

9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative

splicing results in several transcript variants encoding different isoforms. [provided by RefSeq]

UniProtKB/Swiss-Prot: APAF_HUMAN, O14727

Function: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of

pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation

requires ATP. Isoform 6 is less effective in inducing apoptosis

Gene Wiki entry for APAF1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the APAF1 gene

Entrez Gene cytogenetic band: 12q23 Ensembl cytogenetic band: 12q23.1 HGNC cytogenetic band: 12q23

APAF1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12 GeneLoc Exon Structure

GeneLoc location for GC12P097541: (about GC identifiers)

Start:	97,563,209 bp from pter
End:	97,653,342 bp from pter
Size:	90,134 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000012.10 NT_019546.15

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: APAF_HUMAN, O14727 (See protein sequence)

Recommended Name: Apoptotic protease-activating factor 1

Size: 1248 amino acids; 141840 Da

Subunit: Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and

pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively

mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the

Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP

Subcellular location: Cytoplasm

Sequence caution: Sequence=AAK28401.1; Type=Frameshift; Positions=108;

PDB structures from and Proteopedia :

1C15 (3D)

1CWW (3D)

1CY5 (3D)

1Z6T (3D)

2P1H (3D)

2YGS (3D)

3YGS (3D)

Secondary accessions: O43297 Q7Z438 Q9BXZ6 Q9UBZ5 Q9UGN8 Q9UGN9 Q9UGP0 Q9UJ58 Q9UJ59 Q9UJ60 Q9UJ61

Q9UJ62 Q9UJ63 Q9UJ64 Q9UJ65 Q9UJ66 Q9UJ67 Q9UNC9

Alternative splicing: 6 isoforms: O14727-1 O14727-2 O14727-3 O14727-4 O14727-5 O14727-6

Post-translational modifications:

View phosphorylation sites using PhosphoSite²

REFSEQ proteins (5 alternative transcripts):

NP_001151.1 NP_037361.1 NP_863651.1 NP_863658.1 NP_863659.1

ENSEMBL proteins:

ENSP00000341830 ENSP00000349862 ENSP00000353059 ENSP00000334558

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (APAF1)
	Human Recombinant Proteins from Abnova (APAF1)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005622	intracellular	IEA	--
GO:0005737	cytoplasm	IEA	--
GO:0005829	cytosol	TAS	9267021

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Antibodies for APAF1:

	Browse Antibodies Central at Invitrogen
	Millipore Mono- and Polyclonal Antibodies for the study of APAF1
	Sigma-Aldrich Antibody Arrays and Antibodies for APAF1
	R&D Systems Antibodies for APAF1 (APAF-1)
	Cell Signaling Technology (CST) Antibodies for APAF1 (Apaf-1)
	Antibodies from Abcam (APAF1), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (APAF1)
	Novus Biologicals Antibodies for APAF1

Assays for APAF1:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry O14727

ProtoNet protein and cluster: O14727

4 Blocks protein families:

IPB000767 Disease resistance protein signature

IPB001315 CARD interaction domain

IPB001680 G-protein beta WD-40 repeat signature

IPB002182 NB-ARC domain

UniProtKB/Swiss-Prot: APAF_HUMAN, O14727

Domain: The CARD domain mediates interaction with APIP

Similarity: Contains 1 CARD domain

Similarity: Contains 1 NB-ARC domain

Similarity: Contains 13 WD repeats

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (APAF1)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (APAF1)

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 5): NM_013229

Applied Biosystems Silencer® siRNAs for APAF1

Sigma-Aldrich siRNA for APAF1

Sigma-Aldrich shRNA Panels and shRNA for APAF1

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clones in CMV expression vector (see all 5): NM_001160
Myc/DDK tagged cDNA clones in CMV expression vector (see all 5): NM_001160
untagged cDNA clones in CMV expression vector (see all 5): NM_001160

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_181861

UniProtKB/Swiss-Prot: APAF_HUMAN, O14727

Function: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of

pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation

requires ATP. Isoform 6 is less effective in inducing apoptosis

Induction: By E2F and p53 in apoptotic neurons

Genatlas biochemistry entry for APAF1:

cytoplasmic scaffolding apoptotic protease activating factor 1,89kDa,forming a ternary complex

ATP,datp dependent with cytochrome C1 and the initiator of apoptosis caspase 9,not inhibited by

binding to BCL2

15/19 MGI mutant phenotypes (inferred from 4 alleles) (MGI details for Apaf1) (see all 19 ):

	behavior/neurological	cellular	craniofacial	digestive/alimentary
endocrine/exocrine gland	hearing/vestibular/ear	immune system	lethality-postnatal	lethality-prenatal/perinatal
life span-post-weaning/aging	limbs/digits/tail	nervous system	normal	reproductive system

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000166	nucleotide binding	TAS	10383829
GO:0005515	protein binding	IPI	9267021 10949025
GO:0005524	ATP binding	IEA	--
GO:0008656	caspase activator activity	NAS	15009102

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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5/15 Sigma-Aldrich "Your Favorite Gene" Pathways for APAF1 (Your Favorite Gene powered by Ingenuity) (see all 15 )

p53 Signaling
Lymphotoxin &beta
Role of PKR in Interferon Induction and Antiviral Response
Type I Diabetes Mellitus Signaling
Docosahexaenoic Acid (DHA) Signaling

GeneDecks APAF1 for the pathways selected above

About GeneDecksing

Gene Network Central^TM Interacting Genes and Proteins Network for APAF1

5/80 Interacting proteins for APAF1 (ENSP00000353059³ O14727^{1, 2}) via UniProtKB, MINT, and/or STRING (see all 80 )

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
CYCS	P99999¹	STRING (score=.999) EBI-446492, EBI-446479
AVEN	Q9NQS1²	STRING (score=.894) MINT-17186 MINT-17187
CASP3	P42574²	STRING (score=.998) MINT-18036
CASP9	P55211¹^,²	STRING (score=.999) EBI-446492, EBI-516799 MINT-15372 MINT-15373 MINT-24935 MINT-15374 EBI-446492, EBI-516799 MINT-15372 MINT-15373 MINT-24935 MINT-15374
PPP1CA	P62136¹	EBI-446492, EBI-357253

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3 Gene Ontology (GO) biological process terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0007399	nervous system development	TAS	9753320
GO:0008635	activation of caspase activity by cytochrome c	IDA	9267021
GO:0042981	regulation of apoptosis	TAS	10441496

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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for APAF1
10/12 Novoseek chemical compound relationships for APAF1 gene (see all 12 )

Compound	Score	Articles	PubMed IDs for Articles with Shared Sentences (# sentences)
atp	45.61	59	10940292 (4), 11035811 (3), 10364241 (3), 15829969 (2) (see all 23)
noxa	44.89	1	15314285 (1)
crcs	35.95	5	17516584 (3), 17350821 (1), 15378005 (1)
steroid	4.30	1	16555999 (1)
p00001	2.21	2	15633635 (1), 16697107 (1)
calcium	1.50	3	17244527 (3)
lipid	1.13	2	15692060 (1)
oxygen	0.00	2	14598320 (1), 10749135 (1)
superoxide	0.00	1	16046141 (1)
nitric oxide	0.00	4	15492260 (1), 12136132 (1), 12605597 (1)

About this table

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (APAF1)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (APAF1)

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 5): NM_013229

Sigma-Aldrich siRNA for APAF1

Sigma-Aldrich shRNA Panels and shRNA for APAF1

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_001160 NM_013229 NM_181861 NM_181868 NM_181869

REFSEQ mRNAs for APAF1 gene (5 alternative transcripts):

NM_001160.2 NM_013229.2 NM_181861.1 NM_181868.1 NM_181869.1

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_001160 NM_013229 NM_181861 NM_181868 NM_181869

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 5): NM_001160
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 5): NM_001160
                                 untagged cDNA clones in CMV expression vector (see all 5): NM_001160

Additional cDNA sequence:

AB007873.2 AB103079.1 AF013263.1 AF134397.1 AF149794.1 AF248734.1 AJ243003.1 AJ243004.1

AJ243005.1 AJ243006.1 AJ243007.1 AJ243008.1 AJ243009.1 AJ243010.1 AJ243011.1 AJ243048.1

AK307509.1 BC136531.1 BC136532.1 BC150255.1 EF560718.1

10 DOTS entries:

DT.100781260 DT.91749992 DT.95198335 DT.91749981 DT.121196991 DT.91749989 DT.100736551 DT.121196775

DT.95360980 DT.92015250

24/86 AceView cDNA sequences (see all 86 ):

BM472675 AI089181 AJ243004 AF149794 AI017027 AJ243007 NM_181861 AJ243009

NM_181868 AW593134 N51014 CR603841 AI378749 AA285132 AA772429 NM_181869

AJ243006 AJ243003 AF134397 BX280771 T08308 AJ243008 NM_013229 AB103079

highest scoring ESTs for APAF1:

AA640121 AB007873 AF013263 AF134397 AF149794 AI743892 AJ243003 AJ243004 AJ243005 AJ243006

Unigene Cluster for APAF1:

Apoptotic peptidase activating factor 1

Hs.552567 [show with all ESTs]

Unigene Representative Sequence: NM_181861

GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for APAF1

ExUns:	1	^	2	^	3a	·	3b	^	4	^	5	^	6	^	7	^	8	^	9	^	10	^	11a	·	11b	^	12	^	13	^	14a	·	14b
SP1:							-										-		-		-		-		-		-		-
SP2:
SP3:
SP4:
SP5:

About this scheme

ECgene alternative splicing isoforms for APAF1

4 Ensembl transcripts including schematic representations:

ENST00000339433 ENST00000357310 ENST00000359972 ENST00000333991

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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APAF1 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for APAF1

¹ / ² / ³

6 probe-sets matching APAF1 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
211553_x_at²^{, 3}	U133-A	1	1.00	1.00	--	--	AF248734	0.60	1.00	0.82	1
211554_s_at²^{, 3}	U133-A	1	1.00	1.00	--	--	AF149794	0.80	1.00	0.91	1
204859_s_at²^{, 3}	U133-A	1	1.00	1.00	--	--	NM_013229	0.60	1.00	0.82	1
211553_x_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
211554_s_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
204859_s_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: CAGTCGGTCA

SOURCE GeneReport for Unigene cluster: Hs.552567
Expression variation in blood from EXPOLDB for APAF1
UniProtKB/Swiss-Prot: APAF_HUMAN, O14727
Tissue specificity: Ubiquitous. Highest levels of expression in adult spleen and peripheral blood
leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for APAF1 gene from 5/6 species (see all 6 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	APAF1¹	--	apoptotic peptidase activating factor 1	91.77(n) 91.03(a)	482620 XM_539737.2 XP_539737.2
chimpanzee (Pan troglodytes)	APAF1¹	--	apoptotic peptidase activating factor 1	99.63(n) 99.44(a)	452153 XM_509290.2 XP_509290.2
rat (Rattus norvegicus)	Apaf1¹	--	apoptotic peptidase activating factor 1	85.36(n) 87.42(a)	78963 NM_023979.1 NP_076469.1
mouse (Mus musculus)	Apaf1^{1, 5}	10 (48.00 cM)⁵	apoptotic peptidase activating factor 1^{1, 5}	86(n)¹ 87.42(a)¹	11783¹ NM_001042558.1¹ NP_001036023.1¹ AF064071⁵ AJ970309⁵ (see all 14)
chicken (Gallus gallus)	APAF1¹	--	apoptotic peptidase activating factor 1	72.48(n) 70.19(a)	417926 XM_416167.2 XP_416167.2

About this table Species with no ortholog for APAF1

ENSEMBL Gene Tree for APAF1

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
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Paralogs for APAF1 gene

SMU1²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/449 NCBI SNPs in APAF1 are shown (see all 449 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 119)

AB	SNP ID	Valid	Chr 12 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
--	rs6538882^1,2	A,C	97652133(+)	`tctcaC/Tcctct`	5	--	ut3¹ trp³		0	--	--	--	--
--	rs6538881^1,2	A,C	97652132(+)	`ctctcA/Ttcctc`	5	--	ut3¹		0	--	--	--	--
--	rs4450213^1,2	H	97651306(+)	`aagtgC/Atgaga`	5	--	ut3¹		3		EA WA	296
	rs11613534^1,2	H	97589574(+)	`GCAAGC/ATAAGC`	5	D/A	mis¹ int¹		4		EU EA WA	420
	rs2288727^1,2	A,C,F,H,O	97607930(-)	`AATACT/ATGAGT`	5	--	int¹		13		EA NA EU WA MN CSAM	2404
	rs1439124^1,2	A,C,F,H,O	97644201(-)	`TTTCTA/CATCTT`	5	--	int¹		11		NA EU EA WA	976
	rs1439123^1,2	A,C,F,H,O	97600447(-)	`ccctaT/Ctactg`	5	--	int¹		12		MN EU EA WA NA	1164
	rs2840025^1,2	A,C,F,H,O	97603020(-)	`TATTTA/GGAAAT`	5	--	int¹		7		EU EA WA NA	558
	rs2288714^1,2	A,C,F,H,O	97632419(-)	`gaAAAT/CTGTGG`	5	--	int¹		4		EU EA WA	414
	rs2288726^1,2	A,C,F,H,O	97608518(-)	`TTCTTG/TAACAT`	5	--	int¹		17		EA NA EU WA	2950

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HapMap Linkage Disequilibrium images for APAF1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 602233

10/47 Novoseek disease relationships for APAF1 gene (see all 47 )

Disease	Score	Articles	PubMed IDs for Articles with Shared Sentences (# sentences)
plant diseases	46.19	5	11607846 (1), 18362948 (1), 10066601 (1)
metastatic melanoma	44.11	21	11196646 (3), 15009102 (2), 15649154 (2), 15305193 (2) (see all 9)
cancer	41.17	19	11410874 (3), 17317823 (2), 11346470 (2), 18559599 (1) (see all 12)
melanoma	40.95	74	16420245 (7), 15649154 (7), 15009102 (6), 15305193 (5) (see all 20)
tumors	37.83	111	16315248 (10), 11896617 (6), 17541304 (4), 17882496 (4) (see all 38)
allelic imbalance	32.69	5	15378005 (2), 15026369 (1), 15205316 (1)
blast crisis	31.75	3	17603723 (1), 17361096 (1)
ovarian carcinoma	24.25	1	11830553 (1)
necrosis	21.92	6	16532269 (1), 9890966 (1), 10728603 (1), 11872042 (1) (see all 6)
myeloid leukemia	20.67	1	9788601 (1)

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Human Gene Mutation Database: APAF1
Genetic Association Database: APAF1
Human Genome Epidemiology Navigator: APAF1 (6 documents)

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10/414 PubMed articles for APAF1 gene (see all 414 ):

Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. (PubMed id 9267021)^{1, 2, 3, 4} Zou H.... Wang X. (1997)
Three new types of Apaf-1 in mammalian cells. (PubMed id 10441496)^{1, 3, 4} Hahn C.... Stein H. (1999)
Allelic imbalance of 12q22-23 associated with APAF-1 locus correlates with poor disease outcome in cutaneous melanoma. (PubMed id 15026369)^{1, 3, 6} Fujimoto A....Hoon D.S. (2004)
APAF-1-ALT, a novel alternative splicing form of APAF-1, potentially causes impeded ability of undergoing DNA damage-induced apoptosis in the LNCaP human prostate cancer cell line. (PubMed id 12804598)^{1, 3, 4} Ogawa T.... Furukawa T. (2003)
Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation. (PubMed id 10364241)^{1, 3, 4} Saleh A.... Alnemri E.S. (1999)
Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis. (PubMed id 10393175)^{1, 3, 4} Hu Y.... Nunez G. (1999)
Apaf-1 is a transcriptional target for E2F and p53. (PubMed id 11389439)^{1, 3, 4} Moroni M.C.... Helin K. (2001)
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)^{3, 4} Olsen J.V....Mann M. (2006)
Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein. (PubMed id 15262985)^{3, 4} Cho D.H....Jung Y.K. (2004)
Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. (PubMed id 12168954)^{3, 4} Nakajima D.... Nagase T. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
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(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 317	HGNC: 576	AceView: APAF1	Ensembl:ENSG00000120868	euGenes: HUgn317
ECgene: APAF1	H-InvDB: APAF1

(According to HUGE)
About This Section

HUGE: KIAA0413

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
ATLAS Chromosomes in Cancer entry for APAF1	Genetics and Cytogenetics in Oncology and Haematology

Licensable Technologies for APAF1
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for APAF1:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for APAF1-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for APAF1		Antibodies for APAF1
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for APAF1		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

	Recombinant Proteins (APAF1)
	Antibodies (APAF1)
	Chimera RNAi (APAF1)
	Custom Service for Mouse Mab
	Custom Service for Rabbit Pab from Full-length Protein

Antibodies & Assays for APAF1 (Apaf-1)

	Recombinant Proteins (APAF1)
	Antibodies (APAF1)

Search OriGene for APAF1

Search Tocris compounds for APAF1

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Antibodies for APAF1

GeneCards Homepage - Last full update: 2 Jul 2009 Incremental update: 13 Oct 2009

Random Gene From GIFtS :

about GIFtS

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Developed at the Crown Human Genome Center & Weizmann Institute of Science

The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
APAF1 Gene at Home site.
Version 2.41.1
server4.xennexinc.com

APAF1 Gene

protein-coding GIFtS: 69

Aliases &
Descriptions
for APAF1

Summaries
for APAF1

Genomic Location
for APAF1

Proteins
for APAF1

Protein Domains/
Families
for APAF1

Gene Function
for APAF1

Pathways & Interactions
for APAF1

Drugs & Compounds
for APAF1

Transcripts
for APAF1

Expression
for APAF1

Orthologs
for APAF1

Paralogs
for APAF1

SNPs/Variants
for APAF1

Disorders & Mutations
for APAF1

Medical News
for APAF1

Publications
for APAF1

Search for APAF1

Genome Databases showing APAF1

Other Databases showing APAF1

Specialized Databases showing APAF1

Licensable Technologies
for APAF1

Services
for APAF1

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

APAF1 Gene

protein-coding GIFtS: 69

Aliases & Descriptionsfor APAF1

Summariesfor APAF1

Genomic Locationfor APAF1

Proteinsfor APAF1

Protein Domains/Familiesfor APAF1

Gene Functionfor APAF1

Pathways & Interactionsfor APAF1

Drugs & Compoundsfor APAF1

Transcriptsfor APAF1

Expression for APAF1

Orthologsfor APAF1

Paralogsfor APAF1

SNPs/Variantsfor APAF1

Disorders & Mutationsfor APAF1

Medical Newsfor APAF1

Publicationsfor APAF1

Search for APAF1

Genome Databases showing APAF1

Other Databases showing APAF1

Specialized Databases showing APAF1

Licensable Technologiesfor APAF1

Servicesfor APAF1

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for APAF1

Summaries
for APAF1

Genomic Location
for APAF1

Proteins
for APAF1

Protein Domains/
Families
for APAF1

Gene Function
for APAF1

Pathways & Interactions
for APAF1

Drugs & Compounds
for APAF1

Transcripts
for APAF1

Expression
for APAF1

Orthologs
for APAF1

Paralogs
for APAF1

SNPs/Variants
for APAF1

Disorders & Mutations
for APAF1

Medical News
for APAF1

Publications
for APAF1

Licensable Technologies
for APAF1

Services
for APAF1