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AP1G2 Gene

protein-coding   GIFtS: 53

GC14M023098
adaptor-related protein complex 1, gamma 2 subunit
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
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Aliases &
Descriptions
for AP1G2

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section
Aliases
G2AD 1, 2
G2ad 3
Gamma2-adaptin 3
gamma2-adaptin 2
Descriptions
adaptor-related protein complex 1, gamma 2 subunit 2
clathrin-associated/assembly/adaptor protein, large,
gamma-2 2
External Ids
HGNC: 5561
Entrez Gene: 89062
UniProtKB: O758433
Ensembl: ENSG000002139837
Search outside databases for aliases for AP1G2 gene

Previous GC identifers: GC14M021401 GC14M017817 GC14M022018

Summaries
for AP1G2

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

 EntrezGene summary for AP1G2:
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor
complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin
family of proteins is compsed of four classes of molecules named alpha, beta-, beta prime- and
gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric
complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and
vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the
adaptor complexes large subunits family. This protein along with the complex is thought to
function at some trafficking step in the complex pathways between the trans-Golgi network and the
cell surface. There are two alternatively spliced transcript variants of this gene encoding the
same protein. [provided by RefSeq]

UniProtKB/Swiss-Prot: AP1G2_HUMAN, O75843
Function: May function in protein sorting in late endosomes or multivesucular bodies (MVBs).
Involved in MVB-asssisted maturation of hepatitis B virus (HBV)

Gene Wiki entry for AP1G2

Genomic Location
for AP1G2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

 Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the AP1G2 gene  

Entrez Gene cytogenetic band: 14q11.2   Ensembl cytogenetic band:  14q11.2   HGNC cytogenetic band: 14q11.2

AP1G2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14M023098:     (about GC identifiers)

Start:
 23,098,616 bp from pter
End:
 23,107,119 bp from pter
Size:
 8,504 bases
Orientation:
 minus strand
RefSeq DNA sequence:
NC_000014.7  NT_026437.11  

Proteins
for AP1G2

(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

 UniProtKB/Swiss-Prot: AP1G2_HUMAN, O75843 (See protein sequence)
Recommended Name: AP-1 complex subunit gamma-like 2  
Size: 785 amino acids; 87117 Da
Subunit: Probably interacts with AP1S1/Sigma1A-adaptin AP1S2/Sigma1B-adaptin. Probably does not
interact with APB1. Interacts with HBV major surface antigen L. Interacts with HBV core protein C
in a ubiquitin-dependent manner. Binds ubiquitin
Subcellular location: Golgi apparatus membrane; Peripheral membrane protein. Cytoplasmic vesicle
membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note=Mainly
localized to perinuclear vesicular structures. Colocalizes with HBV major surface antigen L and
HBV core protein C in CD63-containing compartments. Colocalizes with HBV major surface antigen L
to cis-Golgi-like structures
Caution: Does not appear to be a subunit of the clathrin-associated adaptor protein complex 1
(AP-1)
Sequence caution: Sequence=AAC67390.1; Type=Frameshift; Positions=713;
PDB structures from and Proteopedia :
2E9G (3D)    
Secondary accessions: O75504

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


    • REFSEQ proteins (2 alternative transcripts): 
    NP_003908.1  NP_536806.1  


    ENSEMBL proteins: 
    ENSP00000312442 ENSP00000380309 


    Human Recombinant Proteins 
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    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (AP1G2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    5/11 Gene Ontology (GO) cellular component terms (links to tree view) (see all 11 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000139 Golgi membrane IEA--
    GO:0005737 cytoplasm IDA18029348
    GO:0005768 endosome IEA--
    GO:0005798 Golgi-associated vesicle IDA9762922
    GO:0005886 plasma membrane IDA18029348
    About this table

    Antibodies for AP1G2: 
    Browse Antibodies Central at Invitrogen
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    Sigma-Aldrich Antibodies for AP1G2
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    Monoclonal and Polyclonal Antibodies from Abnova (AP1G2)
    Novus Biologicals Antibody for AP1G2

    Assays for AP1G2: 
    Browse Invitrogen for biochemical assays
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    Browse biochemical assays available from Enzo Life Sciences

    Protein Domains/
    Families
    for AP1G2

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    5 InterPro domains/families:
     IPR017107 AP1_complex_gsu
     IPR008152 Clathrin_a/b/g-adaptin_app_Ig
     IPR011989 ARM-like
     IPR008153 Clathrin_g-adaptin_app
     IPR002553 Clathrin/coatomer_adapt-like_N


       GeneDecks  AP1G2 for the domains selected above  
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    Graphical View of Domain Structure for InterPro Entry O75843

    ProtoNet protein and cluster: O75843

    3 Blocks protein families:
    IPB002553 Adaptin
    IPB008152 Alpha/gamma adaptin
    IPB008153 Gamma-adaptin


    UniProtKB/Swiss-Prot: AP1G2_HUMAN, O75843
    Similarity: Belongs to the adaptor complexes large subunit family
    Similarity: Contains 1 GAE domain

    Gene Function
    for AP1G2

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section
    Inhib.
    RNA:    		Invitrogen RNAi Products for gene knock-down (AP1G2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (AP1G2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_080545

                  Applied Biosystems Silencer® siRNAs for AP1G2

                  Sigma-Aldrich siRNA for AP1G2  
                         Sigma-Aldrich shRNA for AP1G2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_003917
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_003917
                                     untagged cDNA clones in CMV expression vector (see all 3): AK092855 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_003917

    UniProtKB/Swiss-Prot: AP1G2_HUMAN, O75843
    Function: May function in protein sorting in late endosomes or multivesucular bodies (MVBs).
    Involved in MVB-asssisted maturation of hepatitis B virus (HBV)

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding ISS--
    GO:0008565 protein transporter activity IEA--
    About this table

    Pathways & Interactions
    for AP1G2

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for AP1G2
     Clathrin-dependent protein traffic

       GeneDecks  AP1G2 for the pathways selected above  
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    4 Sigma-Aldrich "Your Favorite Gene" Pathways for  AP1G2  (Your Favorite Gene powered by Ingenuity) 
     Virus Entry via Endocytic Pathways
     GABA Receptor Signaling
     CTLA4 Signaling in Cytotoxic T Lymphocytes
     Clathrin-mediated Endocytosis

       GeneDecks  AP1G2 for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  AP1G2 


    5/17 Interacting proteins for AP1G2 (O758431, 2 ENSP000003124423) via UniProtKB, MINT, and/or STRING (see all 17 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    XRN1Q8IZH21EBI-373637, EBI-372406
    RABGEF1 RABGEF1 (xeno)O189731EBI-373637, EBI-447376
    AP1S1ENSP000003366663STRING (score=.999)
    AP1S2ENSP000003696453STRING (score=.993)
    AP1GBP1ENSP000000052793STRING (score=.992)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006886 intracellular protein transport IEA--
    GO:0016192 vesicle-mediated transport NAS9733768
    GO:0044419 interspecies interaction between organisms IEA--
    About this table

    Drugs & Compounds
    for AP1G2

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for AP1G2

    Transcripts
    for AP1G2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene)
    About This Section
    Inhib.
    RNA:    		Invitrogen RNAi Products for gene knock-down (AP1G2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (AP1G2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_080545

                  Sigma-Aldrich siRNA for AP1G2  
                         Sigma-Aldrich shRNA for AP1G2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_003917  

    REFSEQ mRNAs for AP1G2 gene (2 alternative transcripts): 

    NM_003917.2   NM_080545.1   

    NM_003917  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_003917
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_003917
                                     untagged cDNA clones in CMV expression vector (see all 3): AK092855 

    Additional cDNA sequence: 

    AB015318.1 AF068706.1 AK096034.1 AK123354.1 AK226158.1 AK304682.1 AK307696.1 AK309103.1 
    AK310451.1 BC051833.1 BX161425.1 BX247963.1 CR594047.1 CR600746.1 CR610219.1 CR613895.1 
    CR614596.1 

    24/30 DOTS entries (see all 30 ):

    DT.95225309  DT.456303  DT.95141898  DT.411162  DT.92049578  DT.95318534  DT.97763153  DT.97848227 
    DT.100882759  DT.91890991  DT.100882744  DT.95225251  DT.120750746  DT.100841881  DT.100882738  DT.120750789 
    DT.451129  DT.91702166  DT.95161805  DT.100882741  DT.100882765  DT.100882770  DT.95225297  DT.100882750 

    24/328 AceView cDNA sequences (see all 328 ):

    AL048766 CR614596 BX400236 AW131744 CF455855 AI129936 AI733643 BQ049029 
    AI829725 AI936820 BX384652 CB130470 BG413503 CR615538 F07336 AI827402 
    AW961067 R84470 BM920107 BQ054727 BX247963 BG911312 CR611477 BG993984 

    highest scoring ESTs for AP1G2:

    AF068706 AB015318 BF873263 BG756732 BG759038 BI333758 BI334956 BI765745 BQ709146 BQ937186 

    Unigene Cluster for AP1G2:

    Adaptor-related protein complex 1, gamma 2 subunit
    Hs.343244  [show with all ESTs]
    Unigene Representative Sequence: AF068706


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000308724  ENST00000397120  

    Expression
    for AP1G2

    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    AP1G2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for AP1G2

    1 / 2 / 3

    6 probe-sets matching AP1G2 gene


    		Affymetrix
    probe-set    		 Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    38798_s_at2, 3 U95-A 1 1.00 1.00 0.97 1.00 AI741833 0.60 0.83 0.73 1
    38799_at2, 3 U95-A 1 1.00 1.00 0.98 1.00 AF068706 1.00 0.90 0.95 1

    		201613_s_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    		214341_at*2, 3 U133-A 1 1.00 1.00 -- -- AI741833 0.60 0.83 0.73 1

    		201613_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    		214341_at*2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  AP1G2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AGGGCTGCCA

    SOURCE GeneReport for Unigene cluster: Hs.343244

    Expression variation in blood from EXPOLDB for AP1G2

    UniProtKB/Swiss-Prot: AP1G2_HUMAN, O75843
    Tissue specificity: Expressed in all but one (skeletal muscle) tissues examined

    Orthologs
    for AP1G2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section
    Orthologs for AP1G2 gene from 4 species
    Organism Gene Locus Description Human
    Similarity    		 NCBI accessions
    dog
    (Canis familiaris)    		 AP1G21   -- adaptor-related protein complex 1, gamma 2 subunit 89.31(n)
    90.68(a)    		 480253  XM_537377.2  XP_537377.2 
    chimpanzee
    (Pan troglodytes)    		 AP1G21   -- adaptor-related protein complex 1, gamma 2 subunit 99.66(n)
    99.62(a)    		 467407  XM_001163798.1  XP_001163798.1 
    mouse
    (Mus musculus)    		 Ap1g21, 5 145
    		 adaptor protein complex AP-1, gamma 2 subunit1, 5 86.33(n)1
    87.26(a)1    		 117661  NM_007455.21  NP_031481.11 
     AA1852505  AF0687075  (see all 13)
    fruit fly
    (Drosophila melanogaster)    		 AP-1&ggr;3 8C9 non-selective vesicle coating 57(a)   --
    About this table        Species with no ortholog for AP1G2

    ENSEMBL Gene Tree for AP1G2

    Paralogs
    for AP1G2

    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for AP1G2 gene
    AP1G12  

    SNPs/Variants
    for AP1G2

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section

    		  --

    Disorders & Mutations
    for AP1G2

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    		 OMIM: 603534

    Medical News
    for AP1G2

    (Possibly Related Articles in Doctor's Guide)
    About This Section

    		   --

    Publications
    for AP1G2

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    		10/24 PubMed articles for AP1G2 gene (see all 24 ):

    Search for AP1G2

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing AP1G2

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section
    Entrez Gene: 8906 HGNC: 556 AceView: AP1G2 Ensembl:ENSG00000213983 euGenes: HUgn8906
    ECgene: AP1G2 H-InvDB: AP1G2

    Other Databases showing AP1G2

    (According to HUGE)
    About This Section

    		   --

    Specialized Databases showing AP1G2

    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    		   --

    Licensable Technologies
    for AP1G2

    (Available from WIS Yeda, Salk, Tufts)
    About This Section

    		  --

    Services
    for AP1G2

    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for AP1G2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
    		  Free SNP selection tool



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     Antibodies for AP1G2

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