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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

AP1AR Gene

protein-coding   GIFtS: 45
GCID: GC04P113153

adaptor-related protein complex 1 associated regulatory...

(Previous name: chromosome 4 open reading frame 16 )
(Previous symbol: C4orf16)
 Explore 2 diseases affiliated with
AP1AR via our new
 Human Malady Compendium 
Biological research products
for AP1AR
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Adaptor-Related Protein Complex 1 Associated Regulatory Protein1 2     PRO09711
C4orf161 2 3 5     Chromosome 4 Open Reading Frame 161
2C181 2 5     AP-1 Complex-Associated Regulatory Protein2
GBAR2 3 5     Gamma1-Adaptin Brefeldin A Resistance Protein, Gamma-BAR2
Gamma-BAR3     2c183
Adapter-Related Protein Complex 1-Associated Regulatory Protein2 3     Gamma-BAR3
Gamma-1-Adaptin Brefeldin A Resistance Protein2 3     

External Ids:    HGNC: 288081   Entrez Gene: 554352   Ensembl: ENSG000001386607   OMIM: 6108515   UniProtKB: Q63HQ03   

Export aliases for AP1AR gene to outside databases

Previous GC identifers: GC04P113373 GC04P108884


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: AP1AR_HUMAN, Q63HQ0
Function: Necessary for AP-1 dependent transport between the trans-Golgi network and endosomes. Regulates the membrane
association of AP1G1/Gamma1-adaptin, one of the subunits of the AP-1 adapter complex. The direct interaction with
AP1G1/Gamma1-adaptin attenuates the release of the AP-1 complex from membranes

Gene Wiki entry for AP1AR (C4orf16)


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000004.11  NC_018915.1  NT_016354.19  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for AP1AR
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidAP1AR promoter sequence
   Search SABiosciences Chromatin IP Primers for AP1AR

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat AP1AR


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 4q25   Ensembl cytogenetic band:  4q25   HGNC cytogenetic band: 4q25

AP1AR Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
AP1AR gene location

GeneLoc information about chromosome 4         GeneLoc Exon Structure

GeneLoc location for GC04P113153:  view genomic region     (about GC identifiers)

Start:
113,152,893 bp from pter      End:
113,191,211 bp from pter
Size:
38,319 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: AP1AR_HUMAN, Q63HQ0 (See protein sequence)
Recommended Name: AP-1 complex-associated regulatory protein  
Size: 302 amino acids; 34280 Da
Subunit: Interacts (via coiled-coil domain) with AP1G1 (via GAE domain)
Subcellular location: Golgi apparatus, trans-Golgi network. Late endosome. Early endosome. Note=Localizes to the
juxta-nuclear Golgi region and to tubular structures throughout the cytoplasm, which are highly mobile and cycle
between the juxta-nuclear area and the cell periphery
Sequence caution: Sequence=AAF71037.1; Type=Erroneous initiation;
Secondary accessions: Q96GG6 Q9H0V0 Q9P1L4
Alternative splicing: 2 isoforms:  Q63HQ0-1   Q63HQ0-2   

Explore the universe of human proteins at neXtProt for AP1AR: NX_Q63HQ0

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q63HQ0

  • AP1AR Protein expression data from MOPED and PaxDb:    About this image 
    AP1AR Protein Expression
    REFSEQ proteins (2 alternative transcripts): 
    NP_001121898.1  NP_061039.3  

    ENSEMBL proteins: 
     ENSP00000274000   ENSP00000309023   ENSP00000422529  

    Human Recombinant Protein Products for AP1AR: 
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    Uscn Proteins for AP1AR

    Gene Ontology (GO): 5/6 cellular component terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005730nucleolus IDA--
    GO:0005737cytoplasm IDA--
    GO:0005769early endosome IEA--
    GO:0005770late endosome IEA--
    GO:0005794Golgi apparatus IDA--

    AP1AR for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ProtoNet protein and cluster: Q63HQ0


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: AP1AR_HUMAN, Q63HQ0
    Function: Necessary for AP-1 dependent transport between the trans-Golgi network and endosomes. Regulates the membrane
    association of AP1G1/Gamma1-adaptin, one of the subunits of the AP-1 adapter complex. The direct interaction with
    AP1G1/Gamma1-adaptin attenuates the release of the AP-1 complex from membranes

    Phenotypes:
         1 MGI mutant phenotype (inferred from 1 allele(MGI details for Ap1ar):

     no phenotypic analysis 

    AP1AR for phenotypes           About GeneDecksing

    Animal Models:
         Mouse knock-out Ap1artm1.1Vha for AP1AR
       inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for AP1AR 

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    SwitchGear 3'UTR luciferase reporter plasmidAP1AR 3' UTR sequence
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    In Situ Assay
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    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for AP1AR

    STRING Interaction Network Preview (showing 3 interactants - click image to see more details)

    5 Interacting proteins for AP1AR (Q63HQ03 ENSP000002740004) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    GGA1Q9UJY53, ENSP000003413444I2D: score=2 STRING: ENSP00000341344
    AP1G1O437473, ENSP000003771484I2D: score=1 STRING: ENSP00000377148
    RB1P064003I2D: score=1 
    ELAVL1ENSP000003852694STRING: ENSP00000385269
    --Q8IY973I2D: score=1 
    About this table

    Gene Ontology (GO): 5 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0015031protein transport IEA--
    GO:0034315regulation of Arp2/3 complex-mediated actin nucleation IEA--
    GO:0034613cellular protein localization IEA--
    GO:1900025negative regulation of substrate adhesion-dependent cell spreading IMP--
    GO:2000146negative regulation of cell motility IEA--

    AP1AR for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for AP1AR
    Search CenterWatch for drugs/clinical trials and news about AP1AR 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for AP1AR gene (2 alternative transcripts): 
    NM_001128426.1  NM_018569.4  

    Unigene Cluster for AP1AR:

    Adaptor-related protein complex 1 associated regulatory protein
    Hs.435991  [show with all ESTs]
    Unigene Representative Sequence: NM_018569
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000274000(uc003iaj.4 uc003iak.4) ENST00000510527(uc010imm.1)
    ENST00000502954 ENST00000309703 ENST00000506522 ENST00000512741

    miRNA
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    8/32 QIAGEN miScript miRNA Assays for microRNAs that regulate AP1AR (see all 32):
    hsa-miR-25 hsa-miR-488 hsa-miR-4325 hsa-miR-133a hsa-miR-3613-3p hsa-miR-92b hsa-miR-616 hsa-miR-181b
    SwitchGear 3'UTR luciferase reporter plasmidAP1AR 3' UTR sequence
    Inhib. RNA
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    Additional cDNA sequence: 

    AF116612.1 AK310787.1 AK315299.1 AL136628.1 BC009485.2 BX647702.1 

    4 DOTS entries:

    DT.211868  DT.100778810  DT.95163297  DT.121240087 

    24/64 AceView cDNA sequences (see all 64):

    BE894199 AI632191 BM823928 AI859013 BF115177 AW674667 AI686453 AA345339 
    AI285298 AI492921 BE503347 AW963236 CB131910 AF116612 AA256226 AL514472 
    BM468321 BM974015 AI570945 AA127683 AI065133 CB851616 BG110425 AI394199 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for AP1AR    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10
    SP1:                                -           -                                             
    SP2:                                -           -                 -                           
    SP3:                                -                                                         
    SP4:                                                                                          
    SP5:                                                                                          


    ECgene alternative splicing isoforms for AP1AR

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    AP1AR expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TGGCCCTTTC
    AP1AR Expression
    About this image
    See AP1AR Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for AP1AR

    SOURCE GeneReport for Unigene cluster: Hs.435991
        SABiosciences Custom PCR Arrays for AP1AR

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for AP1AR

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for AP1AR gene from 5/13 species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ap1ar1 , 5 adaptor-related protein complex 1 associated regulatory more1, 5 84(n)1
    91.28(a)1
      3 (56.54 cM)5
    2115561  NM_145964.21  NP_666076.21 
     1278072645 
    chicken
    (Gallus gallus)
    Aves AP1AR1 adaptor-related protein complex 1 associated regulatory more 81.83(n)
    83.95(a)
      422693  XM_420646.3  XP_420646.2 
    lizard
    (Anolis carolinensis)
    Reptilia AP1AR6
    --
    81(a)
    1 ↔ 1
    5(143164385-143176191)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.113772 Xenopus laevis transcribed sequences 80.17(n)    BX844011.1 
    zebrafish
    (Danio rerio)
    Actinopterygii LOC1003322651 AP-1 complex-associated regulatory protein-like 61.17(n)
    58.76(a)
      100332265  XM_002664306.2  XP_002664352.1 


    ENSEMBL Gene Tree for AP1AR (if available)
    TreeFam Gene Tree for AP1AR (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/541 NCBI SNPs in AP1AR are shown (see all 541    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 4 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1378763121,2
    --113150943(+) TTTTTA/GAGCAT 2 -- us2k10--------
    rs1494492911,2
    --113150990(+) TTCTCA/GGAATG 2 -- us2k10--------
    rs1830006551,2
    --113151122(+) ATGCAC/TTTTAA 2 -- us2k10--------
    rs119331821,2
    H--113151175(+) ATTTGA/TATTGC 2 -- us2k10--------
    rs1879691931,2
    --113151261(+) TTTAAC/TAACGG 2 -- us2k10--------
    rs1929936851,2
    --113151682(+) TTCCAA/TAGGAT 2 -- us2k10--------
    rs76994351,2
    C,F,A,H--113151732(+) AATGAC/TTATTT 2 -- us2k1 tfbs322Minor allele frequency- T:0.29NS EA NA WA CSA 2344
    rs777079671,2
    F--113151739(+) ATTTCA/GTTAAT 2 -- us2k12Minor allele frequency- G:0.11WA EA 238
    rs1823936071,2
    --113152133(+) TGTCTA/TGAGGC 2 -- us2k10--------
    rs1878834011,2
    --113152178(+) AGGGCC/TGCGGA 2 -- us2k10--------

    HapMap Linkage Disequilibrium report for AP1AR (113152893 - 113191211 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for AP1AR: --

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    AP1AR for disorders           About GeneDecksing

    OMIM gene information: 610851    OMIM disorders: --

    2 diseases for AP1AR:    About MalaCards
    alcohol dependence    alcoholism

    Human Genome Epidemiology (HuGE) Navigator: AP1AR (1 document)

    Export disorders for AP1AR gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for AP1AR gene, integrated from 9 sources (see all 21):
    (articles sorted by number of sources associating them with AP1AR)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking. (PubMed id 15775984)1, 2, 3 Neubrand V.E.... Simpson J.C. (2005)
    2. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)1, 2 Hillier L.W....Wilson R.K. (2005)
    3. Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry. (PubMed id 15144186)1, 2 Brill L.M....Peters E.C. (2004)
    4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    5. Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. (PubMed id 11230166)1, 2 Wiemann S.... Poustka A. (2001)
    6. Role of AP1 and Gadkin in the traffic of secretory en do-lysosomes. (PubMed id 21525240)1 Laulagnier K....Gruenberg J. (2011)
    7. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
    8. A systematic gene-based screen of chr4q22-q32 identif ies association of a novel susceptibility gene, DKK2, with the quantitative tra it of alcohol dependence symptom counts. (PubMed id 20332099)1 Kalsi G....Riley B.P. (2010)
    9. A novel subtype of AP-1-binding motif within the palm itoylated trans-Golgi network/endosomal accessory protein Gadkin/gamma-BAR. (PubMed id 19965873)1 Maritzen T....Haucke V. (2010)
    10. Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (2009)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 55435 HGNC: 28808 AceView: C4orf16 Ensembl:ENSG00000138660 euGenes: HUgn55435
    ECgene: AP1AR H-InvDB: AP1AR

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for AP1AR Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for AP1AR gene:
    Search GeneIP for patents involving AP1AR

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
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