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ANTXR1 Gene

protein-coding   GIFtS: 59

GC02P069152
anthrax toxin receptor 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
2310008J16Rik 2
2810405N18Rik 2
ATR 1, 2, 3, 5
FLJ10601 1, 2
FLJ11298 2
FLJ21776 1, 2
TEM8 1, 2, 3, 5
Descriptions
Tumor endothelial marker 8 2, 3
anthrax toxin receptor 1 2
External Ids
HGNC: 210141
Entrez Gene: 841682
UniProtKB: Q9H6X23
Ensembl: ENSG000001696047
Search outside databases for aliases for ANTXR1 gene

Previous GC identifers: GC02P069198 GC02P069215

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for ANTXR1:
This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that
has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking
protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The
binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this
receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the
cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt
normal cellular processes. Three alternatively spliced variants that encode different protein
isoforms have been described. [provided by RefSeq]

UniProtKB/Swiss-Prot: ANTR1_HUMAN, Q9H6X2
Function: Cellular role is not yet known

Gene Wiki entry for ANTXR1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ANTXR1 gene  

Entrez Gene cytogenetic band: 2p13.1   Ensembl cytogenetic band:  2p14   HGNC cytogenetic band: 2p13.1

ANTXR1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P069152:     (about GC identifiers)

Start:
69,093,942 bp from pter
End:
69,329,806 bp from pter
Size:
235,865 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_022184.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: ANTR1_HUMAN, Q9H6X2 (See protein sequence)
Recommended Name: Anthrax toxin receptor 1 precursor  
Size: 564 amino acids; 62789 Da
Subunit: Binds to the protective antigen (PA) of Bacillus anthracis. Binding does not occur in the
presence of calcium
Subcellular location: Membrane; Single-pass type I membrane protein (Probable)
Secondary accessions: Q96P02 Q9NVP3
Alternative splicing: 4 isoforms:  Q9H6X2-1   Q9H6X2-2   Q9H6X2-3   Q9H6X2-4   (Experimental confirmation may be lacking for some isoforms)

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (3 alternative transcripts): 
    NP_060623.2  NP_115584.1  NP_444262.1  


    ENSEMBL proteins: 
    ENSP00000377852 ENSP00000310614 ENSP00000386494 ENSP00000301945 ENSP00000386855 ENSP00000387058 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (TEM8)
    Human Recombinant Proteins from Abnova (ANTXR1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016020 membrane IEA--
    GO:0016021 integral to membrane IEA--
    About this table

    Antibodies for ANTXR1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for ANTXR1
    R&D Systems Antibodies for ANTXR1 (TEM8)
    Antibodies from Abcam (TEM8), each with their AbpromiseSM
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    Novus Biologicals Antibodies for ANTXR1

    Assays for ANTXR1: 
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    Browse Kits and Assays available from Millipore
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    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR008400 Anthrax_toxin_rcpt_extracel
     IPR017360 Anthrax_toxin_rcpt_2
     IPR008399 Anthrax_toxin_rcpt_C
     IPR002035 VWF_A


       GeneDecks  ANTXR1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9H6X2

    ProtoNet protein and cluster: Q9H6X2

    2 Blocks protein families:
    IPB002035 Von Willebrand factor type A domain signature
    IPB008399 Anthrax receptor


    UniProtKB/Swiss-Prot: ANTR1_HUMAN, Q9H6X2
    Domain: Binding to PA seems to be effected through the VWA domain
    Similarity: Belongs to the ATR family
    Similarity: Contains 1 VWFA domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ANTXR1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (ANTXR1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_032208

                  Applied Biosystems Silencer® siRNAs for ANTXR1

                  Sigma-Aldrich siRNA and siRNA Panels for ANTXR1  
                         Sigma-Aldrich shRNA for ANTXR1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for ANTXR1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_018153
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_018153
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_018153 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_032208

    UniProtKB/Swiss-Prot: ANTR1_HUMAN, Q9H6X2
    Function: Cellular role is not yet known

    1 MGI phenotypic allele for Antxr1 (no phenotypes)


    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004872 receptor activity IEA--
    GO:0005515 protein binding IPI14507921 16564009
    GO:0046872 metal ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/10 Interacting proteins for ANTXR1 (ENSP000003019453 Q9H6X21) via UniProtKB, MINT, and/or STRING (see all 10 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    LRP6O755811STRING (score=.874) EBI-905643, EBI-910915
    pagA pagA (xeno)P134231EBI-905659, EBI-456868
    ANTXR2ENSP000003061853STRING (score=.905)
    PLXDC1ENSP000003239273STRING (score=.736)
    CD248ENSP000003081173STRING (score=.714)
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for ANTXR1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ANTXR1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (ANTXR1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_032208

                  Sigma-Aldrich siRNA and siRNA Panels for ANTXR1  
                         Sigma-Aldrich shRNA for ANTXR1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_018153  NM_032208  NM_053034  

    REFSEQ mRNAs for ANTXR1 gene (3 alternative transcripts): 

    NM_018153.3   NM_032208.2   NM_053034.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_018153  NM_032208  NM_053034  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_018153
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_018153
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_018153 

    Additional cDNA sequence: 

    AF090095.1 AF279145.2 AF421380.1 AK002160.1 AK025429.1 AK126783.1 AK223273.1 AK292113.1 
    AK309553.1 AL110155.1 BC012074.1 BC012475.1 CR601167.1 CR608949.1 

    23 DOTS entries:

    DT.100030836  DT.119004  DT.97775779  DT.101968313  DT.120969172  DT.309596  DT.120969196  DT.101976518 
    DT.120969200  DT.100821866  DT.100030845  DT.100821863  DT.120969213  DT.75107758  DT.95278535  DT.120969167 
    DT.120969219  DT.421219  DT.75179577  DT.91671089  DT.95125892  DT.100821867  DT.91726528 

    24/346 AceView cDNA sequences (see all 346 ):

    BC012074 Z39069 AW118038 BQ575871 AA424878 CB215475 AI356731 AW296332 
    AI400227 AI379167 AW515125 AI242331 AL542724 CN483575 BX474676 T30707 
    BF793282 CB149116 AW207263 CR608949 AA478681 AA235039 AI018522 CB145982 

    highest scoring ESTs for ANTXR1:

    AA146966 AA025124 AA025204 AA113076 AA126625 AA126731 AA127004 AA130039 AA130040 AA146967 

    Unigene Cluster for ANTXR1:

    Anthrax toxin receptor 1
    Hs.165859  [show with all ESTs]
    Unigene Representative Sequence: NM_032208


    GeneLoc Exon Structure

    5/7 Alternative Splicing Database (ASD) splice patterns (SP) for ANTXR1 (see all 7 )

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11a · 11b · 11c · 11d ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20 ^
    SP1:                                                              -     -                 -     -     -                 -                 -                     
    SP2:                                                              -     -                 -     -     -                 -                                       
    SP3:                                                              -     -                 -     -     -                                                         
    SP4:                                                              -     -                                                                                       
    SP5:                                                                                                                                                            

    ExUns: 21
    SP1:      
    SP2:      
    SP3:      
    SP4:      
    SP5:      

    About this scheme

    ECgene alternative splicing isoforms for ANTXR1

    6 Ensembl transcripts including schematic representations:
    ENST00000394317  ENST00000308181  ENST00000409349  ENST00000303714  ENST00000409608  ENST00000409829  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    ANTXR1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for ANTXR1

    1 / 2 / 3

    11 probe-sets matching ANTXR1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    77387_at2, 3 U95-D 1 1.00 1.00 0.73 1.10 C19059 0.80 1.00 0.91 1

    57718_at2, 3 U95-B 1 1.00 1.00 0.95 1.33 AA146966 1.00 1.00 1.00 1

    51033_at2, 3 U95-B 1 1.00 1.00 0.64 0.58 N20989 0.80 1.00 0.91 1

    224694_at2, 3 U133-B 1 1.00 1.00 -- -- AF279145 0.80 0.88 0.84 1

    220093_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018153 0.60 1.00 0.82 1

    220092_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018153 0.60 1.00 0.82 1

    227660_at2, 3 U133-B 1 1.00 1.00 -- -- AL569575 0.40 1.00 0.76 1

    224694_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    220093_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    220092_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    227660_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  ANTXR1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CTATAGGAGA

    SOURCE GeneReport for Unigene cluster: Hs.165859

    Expression variation in blood from EXPOLDB for ANTXR1

    UniProtKB/Swiss-Prot: ANTR1_HUMAN, Q9H6X2
    Tissue specificity: Highly expressed in tumor endothelial cells but not in normal endothelial cells

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for ANTXR1 gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    ANTXR11   -- anthrax toxin receptor 1 88.54(n)
    95.7(a)
    612601  XM_850334.1  XP_855427.1 
    chimpanzee
    (Pan troglodytes)
    ANTXR11   -- anthrax toxin receptor 1 99.53(n)
    99.82(a)
    470393  XM_525774.2  XP_525774.2 
    cow
    (Bos taurus)
    ANTXR11   -- anthrax toxin receptor 1 91.96(n)
    97.52(a)
    616010  XM_867970.2  XP_873063.2 
    rat
    (Rattus norvegicus)
    Antxr11   -- anthrax toxin receptor 1 86.57(n)
    97.12(a)
    362393  XM_342718.3  XP_342719.3 
    mouse
    (Mus musculus)
    Antxr11, 5 65
    anthrax toxin receptor 11, 5 87.23(n)1
    96.76(a)1
    695381  NM_054041.21  NP_473382.11 
     AA0324075  AF3787625  (see all 12)
    About this table        Species with no ortholog for ANTXR1

    ENSEMBL Gene Tree for ANTXR1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for ANTXR1 gene
    ANTXRL2  ANTXR22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/1200 NCBI SNPs in ANTXR1 are shown (see all 1200 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 408)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs76038501,2
    A,C,F,H69093131(+) GAATCC/TCTAAA 3 -- ng516Minor allele frequency- T:0.13MN EA EU WA 1666
    rs75771321,2
    A,C,F,H69092887(+) CATGCA/GTATGC 3 -- ng514Minor allele frequency- G:0.17EU EA WA 418
    rs75769181,2
    A,C,F,H69092757(+) CACTAA/GGATTC 3 -- ng5112Minor allele frequency- G:0.16EU EA WA NA 1052
    rs102217921,2
    C,F,H69226237(+) CACTTT/GATTGG 3 -- int1 ut314Minor allele frequency- G:0.01EU EA WA 418
    rs48544731,2
    C,F,H69227462(+) TAGTTC/TCAGAG 3 -- ng31 int17Minor allele frequency- T:0.01EU EA WA NA 560
    --
    rs101663701,2
    A,F,H69092373(+) aggatG/Ctcagg 3 -- ng514Minor allele frequency- C:0.13EU EA WA 416
    --
    rs101787841,2
    A,F,H69092372(+) caggaT/Ggtcag 3 -- ng514Minor allele frequency- G:0.14EU EA WA 418
    rs75773981,2
    C,F,H69093099(+) TTCACA/CGGCTG 3 -- ng516Minor allele frequency- C:0.12MN EA EU WA 1650
    rs101899311,2
    F,H69328334(+) CCATCA/GCAACT 1 -- ut31 ese34Minor allele frequency- G:0.01EU EA WA 418
    rs133908121,2
    F,H69093847(+) GGCGCG/TGCCTC 3 -- ut514Minor allele frequency- T:0.06EU EA WA 406
    About this table

    HapMap Linkage Disequilibrium images for ANTXR1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 606410   disorders: 602089  

    3 Novoseek disease relationships for ANTXR1 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    anthrax 88.84 15 12551953 (2), 16285529 (1), 16141341 (1), 17251181 (1) (see all 12)
    edema 39.86 1 17251181 (1)
    tumors 7.75 8 17251181 (3), 14871805 (1), 18931684 (1), 15044490 (1) (see all 6)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/38 PubMed articles for ANTXR1 gene (see all 38 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 84168 HGNC: 21014 AceView: ANTXR1 Ensembl:ENSG00000169604 euGenes: HUgn84168
    ECgene: ANTXR1 H-InvDB: ANTXR1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for ANTXR1 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for ANTXR1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for ANTXR1 Antibodies for ANTXR1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for ANTXR1 Browse small molecules at Sigma-Aldrich
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