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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ANP32E Gene

protein-coding   GIFtS: 50
GCID: GC01M150190

acidic (leucine-rich) nuclear phosphoprotein 32 family,...

 Explore 6 diseases affiliated with
ANP32E via our new
 Human Malady Compendium 
Biological research products
for ANP32E
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Acidic (Leucine-Rich) Nuclear Phosphoprotein 32 Family, Member E1 2     MGC53501
LANP-L1 2 3     Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member E2
LANPL1 2 5     Leucine-Rich Acidic Nuclear Protein Like2
LANP-Like Protein2 3     

External Ids:    HGNC: 166731   Entrez Gene: 816112   Ensembl: ENSG000001434017   OMIM: 6096115   UniProtKB: Q9BTT03   

Export aliases for ANP32E gene to outside databases

Previous GC identifers: GC01M146965 GC01M147407 GC01M147003 GC01M148457 GC01M121570


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: AN32E_HUMAN, Q9BTT0
Function: Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role
in cerebellar development and synaptogenesis process by modulating PP2A activity (By similarity)

Gene Wiki entry for ANP32E


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000001.10  NC_018912.1  NT_004487.19  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ANP32E gene promoter:
         c-Fos   AP-1   MyoD   ZID   c-Jun   Evi-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidANP32E promoter sequence
   Search SABiosciences Chromatin IP Primers for ANP32E

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ANP32E


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1q21.2   Ensembl cytogenetic band:  1q21.2   HGNC cytogenetic band: 1q22

ANP32E Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ANP32E gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M150190:  view genomic region     (about GC identifiers)

Start:
150,190,717 bp from pter      End:
150,208,504 bp from pter
Size:
17,788 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: AN32E_HUMAN, Q9BTT0 (See protein sequence)
Recommended Name: Acidic leucine-rich nuclear phosphoprotein 32 family member E  
Size: 268 amino acids; 30692 Da
Subunit: Interacts with the importin alpha KPNA1 and KPNA2 (By similarity)
Subcellular location: Cytoplasm (By similarity). Nucleus (By similarity)
Secondary accessions: B4E0I6 E9PEA6 Q5TB18 Q8N1S4 Q8WWW9
Alternative splicing: 2 isoforms:  Q9BTT0-1   Q9BTT0-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for ANP32E: NX_Q9BTT0

Post-translational modifications:

  • Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent (By similarity)1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9BTT0

  • ANP32E Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (3 alternative transcripts): 
    NP_001129950.1  NP_001129951.1  NP_112182.1  

    ENSEMBL proteins: 
     ENSP00000324074   ENSP00000358115   ENSP00000393718   ENSP00000431434   ENSP00000358110  
     ENSP00000358111   ENSP00000435215   ENSP00000436529   ENSP00000432684   ENSP00000358112  

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    Uscn Proteins for ANP32E

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus ISS--
    GO:0005737cytoplasm ----
    GO:0016023cytoplasmic membrane-bounded vesicle ISS--


    ANP32E for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ANP32E for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR003603 U2A'_phosphoprotein32A_C
     IPR001611 Leu-rich_rpt
     IPR025875 Leu-rich_rpt_4

    Graphical View of Domain Structure for InterPro Entry Q9BTT0

    ProtoNet protein and cluster: Q9BTT0

    1 Blocks protein family: IPB003603 Leucine-rich-associated

    UniProtKB/Swiss-Prot: AN32E_HUMAN, Q9BTT0
    Similarity: Belongs to the ANP32 family
    Similarity: Contains 4 LRR (leucine-rich) repeats
    Similarity: Contains 1 LRRCT domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: AN32E_HUMAN, Q9BTT0
    Function: Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role
    in cerebellar development and synaptogenesis process by modulating PP2A activity (By similarity)

    miRNA
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    8/56 QIAGEN miScript miRNA Assays for microRNAs that regulate ANP32E (see all 56):
    hsa-miR-3685 hsa-miR-4272 hsa-miR-200a hsa-miR-25 hsa-miR-650 hsa-miR-548l hsa-miR-502-3p hsa-miR-141
    SwitchGear 3'UTR luciferase reporter plasmidANP32E 3' UTR sequence
    Inhib. RNA
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    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0019212phosphatase inhibitor activity ISS--


    ANP32E for ontologies           About GeneDecksing


    2 GenomeRNAi human phenotypes for ANP32E:
     Altered focal adhesion (FA) or  Increased homologous recombina 

    Animal Models:
         Mouse knock-out Anp32etm1Mak for ANP32E
         4 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Anp32e):
     behavior/neurological  mortality/aging  normal  reproductive system 

    ANP32E for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ANP32E

    STRING Interaction Network Preview (showing 5 interactants - click image to see 6)

    5/15 Interacting proteins for ANP32E (Q9BTT03 ENSP000003240744) via UniProtKB, MINT, STRING, and/or I2D (see all 15)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MEPCEQ7L2J03, ENSP000003085464I2D: score=1 STRING: ENSP00000308546
    UCHL5Q9Y5K53, ENSP000003564254I2D: score=1 STRING: ENSP00000356425
    DDA1Q9BW613I2D: score=1 
    FOSP011003I2D: score=1 
    GBASO753233I2D: score=1 
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ANP32E
    Search CenterWatch for drugs/clinical trials and news about ANP32E / AN32E 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ANP32E gene (3 alternative transcripts): 
    NM_001136478.2  NM_001136479.1  NM_030920.3  

    Unigene Cluster for ANP32E:

    Acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
    Hs.656466  [show with all ESTs]
    Unigene Representative Sequence: NM_030920
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000314136(uc010pbt.2 uc010pbu.2 uc001etw.3 uc010pbv.2 uc001etv.4 uc010pbw.1)
    ENST00000369119 ENST00000436748 ENST00000534437 ENST00000369114 ENST00000369115
    ENST00000533654 ENST00000534220 ENST00000532744 ENST00000369116

    miRNA
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    8/56 QIAGEN miScript miRNA Assays for microRNAs that regulate ANP32E (see all 56):
    hsa-miR-3685 hsa-miR-4272 hsa-miR-200a hsa-miR-25 hsa-miR-650 hsa-miR-548l hsa-miR-502-3p hsa-miR-141
    SwitchGear 3'UTR luciferase reporter plasmidANP32E 3' UTR sequence
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    Additional cDNA sequence: 

    AK025624.1 AK092672.1 AK095228.1 AK223469.1 AK303333.1 AK303392.1 AL832664.1 AL832674.1 
    AL833660.1 AY057381.1 BC003380.1 

    12 DOTS entries:

    DT.441530  DT.100807531  DT.91921549  DT.95163066  DT.100037230  DT.100807528  DT.100807530  DT.121401359 
    DT.121401375  DT.40204756  DT.91758320  DT.91758322 

    24/305 AceView cDNA sequences (see all 305):

    BE543560 AW295647 CF136864 AL135007 AL710948 AA854203 AA167570 AA932422 
    CB159648 AA281374 AW504118 BM713617 CR596933 AW169554 BQ049070 AL833660 
    AI682989 AA308903 CK904095 BM974388 AA167571 CA449294 AW504258 AA356832 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for ANP32E    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6 ^ 7 ^ 8a · 8b
    SP1:                          -     -     -     -     -                     
    SP2:                                                                        
    SP3:              -                                                         
    SP4:                                                                        
    SP5:                    -                                                   


    ECgene alternative splicing isoforms for ANP32E

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ANP32E expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: ACAGTGTTAA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See ANP32E Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ANP32E

    SOURCE GeneReport for Unigene cluster: Hs.656466

    UniProtKB/Swiss-Prot: AN32E_HUMAN, Q9BTT0
    Tissue specificity: Expressed in peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen,
    skeletal muscle, liver and kidney

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for ANP32E gene from 8/24 species (see all 24)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves ANP32E1 acidic (leucine-rich) nuclear phosphoprotein 32 family, more 80.18(n)
    86.22(a)
      426109  NM_001006564.2  NP_001006564.2 
    lizard
    (Anolis carolinensis)
    Reptilia ANP32E6
    --
    78(a)
    1 ↔ 1
    GL343697.1(230435-241275)
    African clawed frog
    (Xenopus laevis)
    Amphibia anp32e-prov2 leucine-rich acidic nuclear protein like 77.81(n)    BC043985.1 
    zebrafish
    (Danio rerio)
    Actinopterygii BC056543.12   -- 74.84(n)    BC056543.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Mapmodulin6
    Mapmodulin
    26(a)
    1 → many
    2R(13753827-13759515)
    worm
    (Caenorhabditis elegans)
    Secernentea F33H2.31 Protein F33H2.3 51.12(n)
    45.19(a)
      173372  NM_061221.3  NP_493622.1 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT3G506906
    acidic leucine-rich nuclear phosphoprotein 32-rela...
    21(a)
    1 → many
    3(18834967-18837097)
    rice
    (Oryza sativa)
    Liliopsida --
    --
    acidic leucine-rich nuclear phosphoprotein 32-rela...
    acidic leucine-rich nuclear phosphoprotein 32-rela...
    28(a)
    17(a)
    many ↔ many
    many ↔ many
    3(26370745-26372962)
    7(24983636-24987375)


    ENSEMBL Gene Tree for ANP32E (if available)
    TreeFam Gene Tree for ANP32E (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ANP32E gene
    ANP32D2  ANP32A2  ANP32B2  
    4 SIMAP similar genes for ANP32E using alignment to 8 protein entries:     AN32E_HUMAN (see all proteins):
    ANP32A    ANP32D    ANP32B    ANP32C

    ANP32E for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/279 NCBI SNPs in ANP32E are shown (see all 279    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1496536761,2
    --150190332(+) CCCAGC/TACTTT 3 -- int10--------
    rs1861455851,2
    --150190539(+) CGCCAC/TTGCAC 3 -- int10--------
    rs1454706121,2
    --150190675(+) AAGCCA/GCTAAT 3 -- int10--------
    rs127435681,2
    C,H--150190803(+) AGGGAA/CCAGTC 3 -- ut31 ese35Minor allele frequency- C:0.00NS EA NA 418
    rs1889893391,2
    --150191137(+) GATGAC/TTGATA 3 -- ut310--------
    rs1814047141,2
    --150191214(+) GAATTA/GTTAGG 3 -- ut310--------
    rs1863217951,2
    --150191326(+) GGTCAC/GTTGTA 3 -- ut310--------
    rs1148116411,2
    C,F,--150191364(+) CATCAT/AAATTT 3 -- ut311Minor allele frequency- A:0.06WA 118
    rs1906885811,2
    --150191502(+) AAAACC/TTACAA 3 -- ut310--------
    rs1828167721,2
    --150191531(+) AAAAAA/TTTTAG 3 -- ut310--------

    HapMap Linkage Disequilibrium report for ANP32E (150190717 - 150208504 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for ANP32E: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ANP32E
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ANP32E for disorders           About GeneDecksing

    OMIM gene information: 609611    OMIM disorders: --

    6 diseases for ANP32E:    About MalaCards
    intrahepatic cholangiocarcinoma    cholangiocarcinoma    hepatocellular carcinoma    carcinoma
    malaria    prostatitis


    Export disorders for ANP32E gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ANP32E gene, integrated from 9 sources (see all 36):
    (articles sorted by number of sources associating them with ANP32E)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular cloning and characterization of a novel human gene (ANP32E alias LANPL) from human fetal brain. (PubMed id 12438741)1, 2, 3, 9 Jiang M....Mao Y. (2002)
    2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
    5. Host cell interactome of HIV-1 Rev includes RNA helica ses involved in multiple facets of virus production. (PubMed id 22174317)1 Naji S....Gerace L. (2012)
    6. Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
    7. The interaction between ubiquitin C-terminal hydrolas e 37 and glucose-regulated protein 78 in hepatocellular carcinoma. (PubMed id 21800051)1 Fang Y....Shen X. (2012)
    8. A high-throughput approach for measuring temporal chan ges in the interactome. (PubMed id 22863883)1 Kristensen A.R....Foster L.J. (2012)
    9. Proteome-wide identification of ubiquitylation sites b y conjugation of engineered lysine-less ubiquitin. (PubMed id 22053931)1 Oshikawa K....Nakayama K.I. (2012)
    10. A catalogue of putative HIV-1 protease host cell subst rates. (PubMed id 22944692)1 Impens F....Gevaert K. (2012)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 81611 HGNC: 16673 AceView: ANP32E Ensembl:ENSG00000143401 euGenes: HUgn81611
    ECgene: ANP32E H-InvDB: ANP32E

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for ANP32E Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for ANP32E gene:
    Search GeneIP for patents involving ANP32E

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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